Rh5AG247300

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003521
Physical Location & Seq
Reverse (-)
30612161 .. 30613911
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG247300.1

Sequence Viewer

Length: 819 bp
ATGGACCACCACGACTACAGCCACTCCCACCACCACCACCACCACAGCCAGAGCAGCAGCCACCACCACCATCACCACCGGCACAAGTCCCACTCCGACGGCAACCTCTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGCGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTGCTCCCGCCCCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTCTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCAATTCGGTTGAAGAACAATAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCTGACAGCAATGAGAAAAATGTGGATCAGAAGGCCAAGGTTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGATCAAGTAGCAGTTGAGCGAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTCTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAACCTCCCGCAGATTCAGGTCCTCGTGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTCGAAGCAGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

272

Amino Acids

30.71

Weight (kDa)

9.5

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 87 - 197 4.3e-25 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 252
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 189
AccII CGCG 1 cut(s) 230
AciI CCGC 4 cut(s) 183, 245, 610, 714
AclWI GGATC 4 cut(s) 166, 202, 501, 591
AcsI RAATTY 1 cut(s) 603
AcuI CTGAAG 3 cut(s) 551, 680, 696
AcyI GRCGYC 2 cut(s) 186, 253
AfiI CCNNNNNNNGG 2 cut(s) 255, 410
AgsI TTSAA 5 cut(s) 334, 364, 371, 517, 695
AjnI CCWGG 3 cut(s) 301, 424, 786
AjuI GAANNNNNNNTTGG 4 cut(s) 497, 529, 653, 685
AluBI AGCT 5 cut(s) 359, 390, 542, 565, 680
AluI AGCT 5 cut(s) 359, 390, 542, 565, 680
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 4 cut(s) 166, 202, 501, 591
AoxI GGCC 2 cut(s) 501, 745
ApeKI GCWGC 4 cut(s) 54, 57, 238, 686
ApoI RAATTY 1 cut(s) 603
AspLEI GCGC 2 cut(s) 255, 323
AspS9I GGNCC 3 cut(s) 4, 138, 725
AsuC2I CCSGG 2 cut(s) 250, 408
AsuHPI GGTGA 2 cut(s) 65, 547
AvaII GGWCC 3 cut(s) 4, 138, 725
BanI GGYRCC 1 cut(s) 252
BauI CACGAG 1 cut(s) 729
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 636
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 4 cut(s) 66, 69, 225, 673
BccI CCATC 1 cut(s) 78
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 3 cut(s) 303, 426, 788
BcnI CCSGG 2 cut(s) 250, 408
BcoDI GTCTC 1 cut(s) 354
BfaI CTAG 1 cut(s) 650
BfmI CTRYAG 2 cut(s) 16, 176
BfoI RGCGCY 1 cut(s) 256
BisI GCNGC 5 cut(s) 55, 58, 184, 239, 687
BlsI GCNGC 5 cut(s) 56, 59, 185, 240, 688
Bme1390I CCNGG 5 cut(s) 250, 303, 408, 426, 788
Bme18I GGWCC 3 cut(s) 4, 138, 725
BmgT120I GGNCC 3 cut(s) 4, 138, 725
BmiI GGNNCC 4 cut(s) 139, 140, 254, 260
BmrFI CCNGG 5 cut(s) 250, 303, 408, 426, 788
BmsI GCATC 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 408
Bpu10I CCTNAGC 2 cut(s) 561, 681
BpuMI CCSGG 2 cut(s) 250, 408
BsaHI GRCGYC 2 cut(s) 186, 253
BsaJI CCNNGG 3 cut(s) 248, 302, 504
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 410
Bse118I RCCGGY 2 cut(s) 78, 255
Bse3DI GCAATG 1 cut(s) 484
BseBI CCWGG 3 cut(s) 303, 426, 788
BseDI CCNNGG 3 cut(s) 248, 302, 504
BseLI CCNNNNNNNGG 2 cut(s) 255, 410
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 3 cut(s) 182, 552, 672
BseXI GCAGC 4 cut(s) 66, 69, 225, 673
BsgI GTGCAG 2 cut(s) 363, 672
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 503, 747
BshNI GGYRCC 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 5 cut(s) 79, 250, 256, 262, 407
BslFI GGGAC 2 cut(s) 73, 124
BslI CCNNNNNNNGG 2 cut(s) 255, 410
BsmAI GTCTC 1 cut(s) 354
BsmFI GGGAC 2 cut(s) 73, 124
BsnI GGCC 2 cut(s) 503, 747
Bsp1286I GDGCHC 1 cut(s) 636
Bsp143I GATC 5 cut(s) 171, 194, 233, 493, 583
Bsp68I TCGCGA 1 cut(s) 230
BspACI CCGC 4 cut(s) 183, 245, 610, 714
BspANI GGCC 2 cut(s) 503, 747
BspCNI CTCAG 3 cut(s) 181, 553, 673
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 4 cut(s) 139, 140, 254, 260
BspPI GGATC 4 cut(s) 166, 202, 501, 591
BspQI GCTCTTC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 252
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 484
BsrFI RCCGGY 2 cut(s) 78, 255
BssAI RCCGGY 2 cut(s) 78, 255
BssECI CCNNGG 3 cut(s) 248, 302, 504
BssMI GATC 5 cut(s) 171, 194, 233, 493, 583
BssNI GRCGYC 2 cut(s) 186, 253
BssSI CACGAG 1 cut(s) 729
BssT1I CCWWGG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 729
Bst2UI CCWGG 3 cut(s) 303, 426, 788
Bst4CI ACNGT 2 cut(s) 217, 448
Bst6I CTCTTC 4 cut(s) 113, 166, 311, 656
BstACI GRCGYC 2 cut(s) 186, 253
BstC8I GCNNGC 2 cut(s) 257, 277
BstDEI CTNAG 3 cut(s) 168, 561, 681
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 2 cut(s) 255, 323
BstKTI GATC 5 cut(s) 174, 197, 236, 496, 586
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 5 cut(s) 171, 194, 233, 493, 583
BstMWI GCNNNNNNNGC 4 cut(s) 54, 244, 607, 686
BstNI CCWGG 3 cut(s) 303, 426, 788
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 5 cut(s) 248, 301, 406, 424, 786
BstSFI CTRYAG 2 cut(s) 16, 176
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 4 cut(s) 66, 69, 225, 673
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
BsuRI GGCC 2 cut(s) 503, 747
BtuMI TCGCGA 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 257, 277
CfoI GCGC 2 cut(s) 255, 323
Cfr10I RCCGGY 2 cut(s) 78, 255
Cfr13I GGNCC 3 cut(s) 4, 138, 725
CseI GACGC 2 cut(s) 175, 389
CviAII CATG 2 cut(s) 353, 397
DdeI CTNAG 3 cut(s) 168, 561, 681
DinI GGCGCC 1 cut(s) 254
DpnI GATC 5 cut(s) 173, 196, 235, 495, 585
DpnII GATC 5 cut(s) 171, 194, 233, 493, 583
Eam1104I CTCTTC 4 cut(s) 113, 166, 311, 656
EarI CTCTTC 4 cut(s) 113, 166, 311, 656
Eco130I CCWWGG 1 cut(s) 504
Eco147I AGGCCT 1 cut(s) 747
Eco47I GGWCC 3 cut(s) 4, 138, 725
Eco57I CTGAAG 3 cut(s) 551, 680, 696
EcoO109I RGGNCCY 2 cut(s) 138, 725
EcoRII CCWGG 3 cut(s) 301, 424, 786
EcoT14I CCWWGG 1 cut(s) 504
EgeI GGCGCC 1 cut(s) 254
EheI GGCGCC 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 504
FaeI CATG 2 cut(s) 356, 400
FaiI YATR 6 cut(s) 297, 354, 398, 437, 524, 555
FaqI GGGAC 2 cut(s) 73, 124
FatI CATG 2 cut(s) 352, 396
FauI CCCGC 3 cut(s) 252, 603, 721
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 5 cut(s) 55, 58, 184, 239, 687
Fsp4HI GCNGC 5 cut(s) 55, 58, 184, 239, 687
FspBI CTAG 1 cut(s) 650
GlaI GCGC 2 cut(s) 254, 322
GluI GCNGC 5 cut(s) 55, 58, 184, 239, 687
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 256
HaeIII GGCC 2 cut(s) 503, 747
HapII CCGG 5 cut(s) 79, 250, 256, 262, 407
HgaI GACGC 2 cut(s) 175, 389
HhaI GCGC 2 cut(s) 255, 323
Hin1I GRCGYC 2 cut(s) 186, 253
Hin1II CATG 2 cut(s) 356, 400
Hin6I GCGC 2 cut(s) 253, 321
HinP1I GCGC 2 cut(s) 253, 321
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 3 cut(s) 166, 526, 719
HpaII CCGG 5 cut(s) 79, 250, 256, 262, 407
HphI GGTGA 2 cut(s) 65, 547
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 4 cut(s) 97, 171, 498, 531
Hpy188III TCNNGA 4 cut(s) 229, 560, 650, 731
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 6 cut(s) 101, 151, 154, 191, 194, 230
HpyAV CCTTC 5 cut(s) 493, 526, 737, 760, 768
HpyCH4III ACNGT 2 cut(s) 217, 448
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 380, 629, 689
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 244, 607, 686
HpyF3I CTNAG 3 cut(s) 168, 561, 681
HpySE526I ACGT 1 cut(s) 146
Hsp92I GRCGYC 2 cut(s) 186, 253
Hsp92II CATG 2 cut(s) 356, 400
HspAI GCGC 2 cut(s) 253, 321
KasI GGCGCC 1 cut(s) 252
KflI GGGWCCC 1 cut(s) 138
KroI GCCGGC 1 cut(s) 255
KroNI GCCGGC 1 cut(s) 257
Kzo9I GATC 5 cut(s) 171, 194, 233, 493, 583
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 5 cut(s) 246, 264, 427, 562, 769
Lsp1109I GCAGC 4 cut(s) 66, 69, 225, 673
LweI GCATC 1 cut(s) 616
MaeI CTAG 1 cut(s) 650
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 1 cut(s) 392
MalI GATC 5 cut(s) 173, 196, 235, 495, 585
MbiI CCGCTC 1 cut(s) 183
MboI GATC 5 cut(s) 171, 194, 233, 493, 583
MboII GAAGA 6 cut(s) 100, 147, 153, 328, 346, 673
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 1 cut(s) 636
MluCI AATT 4 cut(s) 324, 438, 519, 603
Mly113I GGCGCC 1 cut(s) 253
MlyI GAGTC 2 cut(s) 160, 535
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 7 cut(s) 116, 169, 414, 657, 676, 720, 738
MroNI GCCGGC 1 cut(s) 255
MseI TTAA 2 cut(s) 510, 817
MspI CCGG 5 cut(s) 79, 250, 256, 262, 407
MspR9I CCNGG 5 cut(s) 250, 303, 408, 426, 788
MvaI CCWGG 3 cut(s) 303, 426, 788
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 4 cut(s) 54, 244, 607, 686
NaeI GCCGGC 1 cut(s) 257
NarI GGCGCC 1 cut(s) 253
NciI CCSGG 2 cut(s) 250, 408
NdeII GATC 5 cut(s) 171, 194, 233, 493, 583
NgoMIV GCCGGC 1 cut(s) 255
NlaIII CATG 2 cut(s) 356, 400
NlaIV GGNNCC 4 cut(s) 139, 140, 254, 260
NmeAIII GCCGAG 1 cut(s) 772
NruI TCGCGA 1 cut(s) 230
NspI RCATGY 1 cut(s) 400
PceI AGGCCT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PdiI GCCGGC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 719
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 5 cut(s) 56, 59, 185, 240, 688
PleI GAGTC 2 cut(s) 160, 534
PluTI GGCGCC 1 cut(s) 256
PpsI GAGTC 2 cut(s) 160, 534
PpuMI RGGWCCY 2 cut(s) 138, 725
Psp5II RGGWCCY 2 cut(s) 138, 725
Psp6I CCWGG 3 cut(s) 301, 424, 786
PspGI CCWGG 3 cut(s) 301, 424, 786
PspN4I GGNNCC 4 cut(s) 139, 140, 254, 260
PspPI GGNCC 3 cut(s) 4, 138, 725
PspPPI RGGWCCY 2 cut(s) 138, 725
PsuI RGATCY 1 cut(s) 171
RruI TCGCGA 1 cut(s) 230
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 311
SaqAI TTAA 2 cut(s) 510, 817
SatI GCNGC 5 cut(s) 55, 58, 184, 239, 687
Sau3AI GATC 5 cut(s) 171, 194, 233, 493, 583
Sau96I GGNCC 3 cut(s) 4, 138, 725
SchI GAGTC 2 cut(s) 160, 535
ScrFI CCNGG 5 cut(s) 250, 303, 408, 426, 788
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 1 cut(s) 616
SfcI CTRYAG 2 cut(s) 16, 176
SfoI GGCGCC 1 cut(s) 254
SgrDI CGTCGACG 1 cut(s) 188
SinI GGWCC 3 cut(s) 4, 138, 725
Sse9I AATT 4 cut(s) 324, 438, 519, 603
SseBI AGGCCT 1 cut(s) 747
SsiI CCGC 4 cut(s) 183, 245, 610, 714
SspDI GGCGCC 1 cut(s) 252
SspMI CTAG 1 cut(s) 650
StuI AGGCCT 1 cut(s) 747
StyD4I CCNGG 5 cut(s) 248, 301, 406, 424, 786
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 217, 448
TaiI ACGT 1 cut(s) 149
TaqI TCGA 6 cut(s) 164, 189, 197, 203, 267, 804
TasI AATT 4 cut(s) 324, 438, 519, 603
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 1 cut(s) 719
Tru1I TTAA 2 cut(s) 510, 817
Tru9I TTAA 2 cut(s) 510, 817
TseI GCWGC 4 cut(s) 54, 57, 238, 686
TspDTI ATGAA 2 cut(s) 312, 774
TspGWI ACGGA 4 cut(s) 111, 207, 214, 563
VpaK11BI GGWCC 3 cut(s) 4, 138, 725
XapI RAATTY 1 cut(s) 603
XbaI TCTAGA 1 cut(s) 649
XceI RCATGY 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.