Rorug02G0490400

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003521
Physical Location & Seq
Forward (+)
61938543 .. 61939351
809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0490400.1

Sequence Viewer

Length: 597 bp
ATGGAGCTACAAACCCAGCAACAAATCGAGGATGGAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACAGATTTCTGGCGAAGCCGTGCCAGGTGGGTTCCTACTCCAGATCAAATAAGGATCCTCAGGGATCTTTACTACGACAAGGGAGTTAAGACCCCAACTACAGAGCAGATTCACGAGATCTGTCTCCAGCTGCAACAGTATGGACAGGTTGAGGGCAAGAACATTTATTTTTGGTTCCAGAATGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGAGGACAATCTTCCATGGAACAACGAGGAGGAGTTCGCCAGGAGTTTGAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTATGGTAACCCGAAGACTACTTCCGAGGAAGGTAGCGCATATGATTACTATTTCGGTGGCGCAGGCGGTTACAACCGTGGATCTCCAGTTTCTCTTGAGCTCAGCCTCAACCCATTCGGAGCTACTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

198

Amino Acids

21.76

Weight (kDa)

5.11

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 4.1e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
AciI CCGC 1 cut(s) 531
AclWI GGATC 6 cut(s) 118, 131, 141, 330, 343, 553
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 2 cut(s) 329, 452
AgsI TTSAA 1 cut(s) 428
AhlI ACTAGT 1 cut(s) 314
AjnI CCWGG 3 cut(s) 92, 322, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
AluBI AGCT 4 cut(s) 7, 199, 565, 587
AluI AGCT 4 cut(s) 7, 199, 565, 587
Alw21I GWGCWC 2 cut(s) 453, 567
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 6 cut(s) 118, 131, 141, 330, 343, 553
Ama87I CYCGRG 1 cut(s) 261
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 3 cut(s) 50, 199, 293
Asp700I GAANNNNTTC 2 cut(s) 316, 432
AspLEI GCGC 2 cut(s) 503, 527
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 357
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 306, 453
BamHI GGATCC 2 cut(s) 123, 335
BanII GRGCYC 2 cut(s) 262, 567
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 2 cut(s) 453, 567
BbvI GCAGC 3 cut(s) 62, 186, 280
BccI CCATC 1 cut(s) 26
BceAI ACGGC 2 cut(s) 72, 469
BciT130I CCWGG 3 cut(s) 94, 324, 419
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 168
BglII AGATCT 1 cut(s) 186
BisI GCNGC 3 cut(s) 51, 200, 294
BlpI GCTNAGC 1 cut(s) 566
BlsI GCNGC 3 cut(s) 52, 201, 295
Bme1390I CCNGG 4 cut(s) 55, 94, 324, 419
Bme18I GGWCC 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 5 cut(s) 102, 125, 245, 337, 358
BmrFI CCNGG 4 cut(s) 55, 94, 324, 419
BpiI GAAGAC 1 cut(s) 485
BpmI CTGGAG 4 cut(s) 93, 179, 399, 534
Bpu1102I GCTNAGC 1 cut(s) 566
BpuEI CTTGAG 2 cut(s) 282, 581
BpuMI CCSGG 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 462
BsaJI CCNNGG 4 cut(s) 306, 393, 489, 541
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 452
Bse1I ACTGG 4 cut(s) 334, 367, 376, 551
Bse21I CCTNAGG 1 cut(s) 128
BseBI CCWGG 3 cut(s) 94, 324, 419
BseDI CCNNGG 4 cut(s) 306, 393, 489, 541
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 2 cut(s) 329, 452
BseMII CTCAG 2 cut(s) 142, 580
BseNI ACTGG 4 cut(s) 334, 367, 376, 551
BseRI GAGGAG 2 cut(s) 420, 423
BseSI GKGCMC 2 cut(s) 306, 453
BseXI GCAGC 3 cut(s) 62, 186, 280
BseYI CCCAGC 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 453, 567
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 329, 452
BsmAI GTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 4 cut(s) 262, 306, 453, 567
Bsp143I GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
Bsp1720I GCTNAGC 1 cut(s) 566
Bsp19I CCATGG 2 cut(s) 306, 393
BspACI CCGC 1 cut(s) 531
BspCNI CTCAG 2 cut(s) 141, 579
BspLI GGNNCC 5 cut(s) 102, 125, 245, 337, 358
BspPI GGATC 6 cut(s) 118, 131, 141, 330, 343, 553
BsrI ACTGG 4 cut(s) 334, 367, 376, 551
BssECI CCNNGG 4 cut(s) 306, 393, 489, 541
BssMI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
BssNI GRCGYC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 306, 393
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 3 cut(s) 94, 324, 419
Bst4CI ACNGT 2 cut(s) 207, 542
Bst6I CTCTTC 2 cut(s) 272, 438
BstACI GRCGYC 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 2 cut(s) 128, 566
BstDSI CCRYGG 3 cut(s) 306, 393, 541
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 1 cut(s) 37
BstHHI GCGC 2 cut(s) 503, 527
BstKTI GATC 7 cut(s) 115, 126, 136, 189, 338, 346, 548
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
BstNI CCWGG 3 cut(s) 94, 324, 419
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 4 cut(s) 53, 92, 322, 417
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 2 cut(s) 306, 453
BstV1I GCAGC 3 cut(s) 62, 186, 280
BstV2I GAAGAC 1 cut(s) 485
BstX2I RGATCY 5 cut(s) 123, 133, 186, 335, 545
BstYI RGATCY 5 cut(s) 123, 133, 186, 335, 545
Bsu36I CCTNAGG 1 cut(s) 128
BtgI CCRYGG 3 cut(s) 306, 393, 541
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 529
CfoI GCGC 2 cut(s) 503, 527
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 1 cut(s) 327
CviAII CATG 2 cut(s) 307, 394
CviJI RGCY 9 cut(s) 7, 53, 87, 199, 260, 293, 565, 570, 587
CviKI_1 RGCY 9 cut(s) 7, 53, 87, 199, 260, 293, 565, 570, 587
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 2 cut(s) 128, 566
DpnI GATC 7 cut(s) 114, 125, 135, 188, 337, 345, 547
DpnII GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
Eam1104I CTCTTC 2 cut(s) 272, 438
EarI CTCTTC 2 cut(s) 272, 438
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 2 cut(s) 306, 393
Eco24I GRGCYC 2 cut(s) 262, 567
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 565
Eco81I CCTNAGG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 565
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 3 cut(s) 92, 322, 417
EcoT14I CCWWGG 2 cut(s) 306, 393
EcoT38I GRGCYC 2 cut(s) 262, 567
ErhI CCWWGG 2 cut(s) 306, 393
FaeI CATG 2 cut(s) 310, 397
FaiI YATR 6 cut(s) 210, 308, 395, 468, 505, 507
FatI CATG 2 cut(s) 306, 393
FauNDI CATATG 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 51, 200, 294
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 2 cut(s) 262, 567
Fsp4HI GCNGC 3 cut(s) 51, 200, 294
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 502, 526
GluI GCNGC 3 cut(s) 51, 200, 294
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 4 cut(s) 93, 179, 399, 534
HapII CCGG 1 cut(s) 54
HhaI GCGC 2 cut(s) 503, 527
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 2 cut(s) 310, 397
Hin6I GCGC 2 cut(s) 501, 525
HinP1I GCGC 2 cut(s) 501, 525
HinfI GANTC 1 cut(s) 178
HpaII CCGG 1 cut(s) 54
Hpy166II GTNNAC 2 cut(s) 360, 451
Hpy188I TCNGA 2 cut(s) 490, 584
Hpy188III TCNNGA 5 cut(s) 110, 182, 247, 263, 560
Hpy8I GTNNAC 2 cut(s) 360, 451
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 2 cut(s) 207, 542
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 3 cut(s) 202, 285, 451
HpyF3I CTNAG 2 cut(s) 128, 566
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 2 cut(s) 310, 397
HspAI GCGC 2 cut(s) 501, 525
Kzo9I GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
LmnI GCTCC 2 cut(s) 4, 584
Lsp1109I GCAGC 3 cut(s) 62, 186, 280
MaeI CTAG 1 cut(s) 315
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 2 cut(s) 470, 533
MalI GATC 7 cut(s) 114, 125, 135, 188, 337, 345, 547
MboI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
MboII GAAGA 4 cut(s) 289, 381, 425, 490
MflI RGATCY 5 cut(s) 123, 133, 186, 335, 545
MhlI GDGCHC 4 cut(s) 262, 306, 453, 567
MluCI AATT 1 cut(s) 349
MroXI GAANNNNTTC 2 cut(s) 316, 432
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 4 cut(s) 55, 94, 324, 419
MvaI CCWGG 3 cut(s) 94, 324, 419
NciI CCSGG 1 cut(s) 55
NcoI CCATGG 2 cut(s) 306, 393
NdeI CATATG 1 cut(s) 505
NdeII GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
NlaIII CATG 2 cut(s) 310, 397
NlaIV GGNNCC 5 cut(s) 102, 125, 245, 337, 358
PaeR7I CTCGAG 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdmI GAANNNNTTC 2 cut(s) 316, 432
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 3 cut(s) 52, 201, 295
Psp124BI GAGCTC 1 cut(s) 567
Psp6I CCWGG 3 cut(s) 92, 322, 417
PspEI GGTNACC 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 3 cut(s) 92, 322, 417
PspN4I GGNNCC 5 cut(s) 102, 125, 245, 337, 358
PspPI GGNCC 1 cut(s) 357
PsuI RGATCY 5 cut(s) 123, 133, 186, 335, 545
PvuII CAGCTG 1 cut(s) 199
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
SacI GAGCTC 1 cut(s) 567
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 3 cut(s) 51, 200, 294
Sau3AI GATC 7 cut(s) 112, 123, 133, 186, 335, 343, 545
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 4 cut(s) 55, 94, 324, 419
SduI GDGCHC 4 cut(s) 262, 306, 453, 567
SetI ASST 9 cut(s) 9, 98, 201, 219, 284, 465, 499, 567, 589
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 357
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 3 cut(s) 261, 297, 560
SmoI CTYRAG 3 cut(s) 261, 297, 560
SpeI ACTAGT 1 cut(s) 314
Sse9I AATT 1 cut(s) 349
SsiI CCGC 1 cut(s) 531
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 4 cut(s) 53, 92, 322, 417
StyI CCWWGG 2 cut(s) 306, 393
TaaI ACNGT 2 cut(s) 207, 542
TaiI ACGT 1 cut(s) 465
TaqI TCGA 2 cut(s) 27, 262
TasI AATT 1 cut(s) 349
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 1 cut(s) 367
TseI GCWGC 3 cut(s) 50, 199, 293
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 1 cut(s) 367
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 357
XhoI CTCGAG 1 cut(s) 261
XmnI GAANNNNTTC 2 cut(s) 316, 432
XspI CTAG 1 cut(s) 315
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.