Rh5DG256100

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003521
Physical Location & Seq
Reverse (-)
30125653 .. 30127368
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG256100.1

Sequence Viewer

Length: 825 bp
ATGGACCACCACCACGACTACAGCCAGAGCAGCAGCCACCACCACCACCACAGCCAGAGCAGCAGCCACCACCACCATCACCACCGGCACAAGTCCCACTCCGACGGCAACCTCTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGCGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTGCTCCCGCCCCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTCTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCGATTCGGTTGCAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCTGACAGCAATGAGAAAAATGTGGATCAGAAGGCCAAGGTTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGATCAAGTAGCAGTTGAGCGAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTCTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAACCTCCCGCAGATGCAGGTCCTCGTGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTTGAAGCAGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

274

Amino Acids

30.83

Weight (kDa)

9.44

Isoelectric Point (pI)

55.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 89 - 199 2e-24 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 189
AccI GTMKAC 1 cut(s) 195
AccII CGCG 2 cut(s) 236, 329
AciI CCGC 4 cut(s) 189, 251, 616, 720
AclWI GGATC 4 cut(s) 172, 208, 507, 597
AcsI RAATTY 1 cut(s) 609
AcuI CTGAAG 3 cut(s) 557, 686, 702
AcyI GRCGYC 2 cut(s) 192, 259
AfiI CCNNNNNNNGG 2 cut(s) 261, 416
AgsI TTSAA 5 cut(s) 370, 377, 523, 701, 812
AjnI CCWGG 3 cut(s) 307, 430, 792
AjuI GAANNNNNNNTTGG 4 cut(s) 503, 535, 659, 691
AluBI AGCT 5 cut(s) 365, 396, 548, 571, 686
AluI AGCT 5 cut(s) 365, 396, 548, 571, 686
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 1 cut(s) 360
AlwI GGATC 4 cut(s) 172, 208, 507, 597
AoxI GGCC 2 cut(s) 507, 751
ApeKI GCWGC 6 cut(s) 30, 33, 60, 63, 244, 692
ApoI RAATTY 1 cut(s) 609
AspLEI GCGC 2 cut(s) 261, 329
AspS9I GGNCC 3 cut(s) 4, 144, 731
AsuC2I CCSGG 2 cut(s) 256, 414
AsuHPI GGTGA 2 cut(s) 71, 553
AvaII GGWCC 3 cut(s) 4, 144, 731
BanI GGYRCC 1 cut(s) 258
BauI CACGAG 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 642
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 6 cut(s) 42, 45, 72, 75, 231, 679
BccI CCATC 1 cut(s) 84
BceAI ACGGC 1 cut(s) 121
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 319, 353
BciT130I CCWGG 3 cut(s) 309, 432, 794
BcnI CCSGG 2 cut(s) 256, 414
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 1 cut(s) 656
BfmI CTRYAG 2 cut(s) 19, 182
BfoI RGCGCY 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 719
BisI GCNGC 7 cut(s) 31, 34, 61, 64, 190, 245, 693
BlsI GCNGC 7 cut(s) 32, 35, 62, 65, 191, 246, 694
Bme1390I CCNGG 5 cut(s) 256, 309, 414, 432, 794
Bme18I GGWCC 3 cut(s) 4, 144, 731
BmgT120I GGNCC 3 cut(s) 4, 144, 731
BmiI GGNNCC 4 cut(s) 145, 146, 260, 266
BmrFI CCNGG 5 cut(s) 256, 309, 414, 432, 794
BmsI GCATC 2 cut(s) 622, 715
BpiI GAAGAC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 414
Bpu10I CCTNAGC 2 cut(s) 567, 687
BpuMI CCSGG 2 cut(s) 256, 414
BsaHI GRCGYC 2 cut(s) 192, 259
BsaJI CCNNGG 3 cut(s) 254, 308, 510
Bsc4I CCNNNNNNNGG 2 cut(s) 261, 416
Bse118I RCCGGY 2 cut(s) 84, 261
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 3 cut(s) 309, 432, 794
BseDI CCNNGG 3 cut(s) 254, 308, 510
BseLI CCNNNNNNNGG 2 cut(s) 261, 416
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 188, 558, 678
BseXI GCAGC 6 cut(s) 42, 45, 72, 75, 231, 679
BsgI GTGCAG 2 cut(s) 369, 678
Bsh1236I CGCG 2 cut(s) 236, 329
BshFI GGCC 2 cut(s) 509, 753
BshNI GGYRCC 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 5 cut(s) 85, 256, 262, 268, 413
BslFI GGGAC 2 cut(s) 79, 130
BslI CCNNNNNNNGG 2 cut(s) 261, 416
BsmAI GTCTC 1 cut(s) 360
BsmFI GGGAC 2 cut(s) 79, 130
BsnI GGCC 2 cut(s) 509, 753
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 5 cut(s) 177, 200, 239, 499, 589
Bsp68I TCGCGA 1 cut(s) 236
BspACI CCGC 4 cut(s) 189, 251, 616, 720
BspANI GGCC 2 cut(s) 509, 753
BspCNI CTCAG 3 cut(s) 187, 559, 679
BspFNI CGCG 2 cut(s) 236, 329
BspLI GGNNCC 4 cut(s) 145, 146, 260, 266
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 4 cut(s) 172, 208, 507, 597
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 1 cut(s) 258
BsrBI CCGCTC 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 490
BsrFI RCCGGY 2 cut(s) 84, 261
BssAI RCCGGY 2 cut(s) 84, 261
BssECI CCNNGG 3 cut(s) 254, 308, 510
BssMI GATC 5 cut(s) 177, 200, 239, 499, 589
BssNI GRCGYC 2 cut(s) 192, 259
BssSI CACGAG 1 cut(s) 735
BssT1I CCWWGG 1 cut(s) 510
Bst2BI CACGAG 1 cut(s) 735
Bst2UI CCWGG 3 cut(s) 309, 432, 794
Bst4CI ACNGT 3 cut(s) 223, 347, 454
Bst6I CTCTTC 4 cut(s) 119, 172, 317, 662
BstACI GRCGYC 2 cut(s) 192, 259
BstC8I GCNNGC 2 cut(s) 263, 283
BstDEI CTNAG 3 cut(s) 174, 567, 687
BstFNI CGCG 2 cut(s) 236, 329
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 2 cut(s) 261, 329
BstKTI GATC 5 cut(s) 180, 203, 242, 502, 592
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 5 cut(s) 177, 200, 239, 499, 589
BstMWI GCNNNNNNNGC 5 cut(s) 30, 60, 250, 613, 692
BstNI CCWGG 3 cut(s) 309, 432, 794
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 5 cut(s) 254, 307, 412, 430, 792
BstSFI CTRYAG 2 cut(s) 19, 182
BstUI CGCG 2 cut(s) 236, 329
BstV1I GCAGC 6 cut(s) 42, 45, 72, 75, 231, 679
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 2 cut(s) 509, 753
BtuMI TCGCGA 1 cut(s) 236
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 2 cut(s) 263, 283
CfoI GCGC 2 cut(s) 261, 329
Cfr10I RCCGGY 2 cut(s) 84, 261
Cfr13I GGNCC 3 cut(s) 4, 144, 731
CseI GACGC 2 cut(s) 181, 395
CviAII CATG 2 cut(s) 359, 403
DdeI CTNAG 3 cut(s) 174, 567, 687
DinI GGCGCC 1 cut(s) 260
DpnI GATC 5 cut(s) 179, 202, 241, 501, 591
DpnII GATC 5 cut(s) 177, 200, 239, 499, 589
Eam1104I CTCTTC 4 cut(s) 119, 172, 317, 662
EarI CTCTTC 4 cut(s) 119, 172, 317, 662
Eco130I CCWWGG 1 cut(s) 510
Eco147I AGGCCT 1 cut(s) 753
Eco47I GGWCC 3 cut(s) 4, 144, 731
Eco57I CTGAAG 3 cut(s) 557, 686, 702
EcoO109I RGGNCCY 2 cut(s) 144, 731
EcoRII CCWGG 3 cut(s) 307, 430, 792
EcoT14I CCWWGG 1 cut(s) 510
EgeI GGCGCC 1 cut(s) 260
EheI GGCGCC 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 2 cut(s) 362, 406
FaiI YATR 6 cut(s) 303, 360, 404, 443, 530, 561
FaqI GGGAC 2 cut(s) 79, 130
FatI CATG 2 cut(s) 358, 402
FauI CCCGC 3 cut(s) 258, 609, 727
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 7 cut(s) 31, 34, 61, 64, 190, 245, 693
Fsp4HI GCNGC 7 cut(s) 31, 34, 61, 64, 190, 245, 693
FspBI CTAG 1 cut(s) 656
GlaI GCGC 2 cut(s) 260, 328
GluI GCNGC 7 cut(s) 31, 34, 61, 64, 190, 245, 693
GsuI CTGGAG 1 cut(s) 414
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 2 cut(s) 509, 753
HapII CCGG 5 cut(s) 85, 256, 262, 268, 413
HgaI GACGC 2 cut(s) 181, 395
HhaI GCGC 2 cut(s) 261, 329
Hin1I GRCGYC 2 cut(s) 192, 259
Hin1II CATG 2 cut(s) 362, 406
Hin6I GCGC 2 cut(s) 259, 327
HinP1I GCGC 2 cut(s) 259, 327
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 3 cut(s) 172, 331, 532
HpaII CCGG 5 cut(s) 85, 256, 262, 268, 413
HphI GGTGA 2 cut(s) 71, 553
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 4 cut(s) 103, 177, 504, 537
Hpy188III TCNNGA 4 cut(s) 235, 566, 656, 737
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 6 cut(s) 107, 157, 160, 197, 200, 236
HpyAV CCTTC 5 cut(s) 499, 532, 743, 766, 774
HpyCH4III ACNGT 3 cut(s) 223, 347, 454
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 5 cut(s) 340, 386, 635, 695, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 60, 250, 613, 692
HpyF3I CTNAG 3 cut(s) 174, 567, 687
HpySE526I ACGT 1 cut(s) 152
Hsp92I GRCGYC 2 cut(s) 192, 259
Hsp92II CATG 2 cut(s) 362, 406
HspAI GCGC 2 cut(s) 259, 327
KasI GGCGCC 1 cut(s) 258
KflI GGGWCCC 1 cut(s) 144
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 5 cut(s) 177, 200, 239, 499, 589
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 5 cut(s) 252, 270, 433, 568, 775
Lsp1109I GCAGC 6 cut(s) 42, 45, 72, 75, 231, 679
LweI GCATC 2 cut(s) 622, 715
MaeI CTAG 1 cut(s) 656
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 398
MalI GATC 5 cut(s) 179, 202, 241, 501, 591
MbiI CCGCTC 1 cut(s) 189
MboI GATC 5 cut(s) 177, 200, 239, 499, 589
MboII GAAGA 5 cut(s) 106, 153, 159, 334, 679
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 3 cut(s) 444, 525, 609
Mly113I GGCGCC 1 cut(s) 259
MlyI GAGTC 2 cut(s) 166, 541
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 7 cut(s) 122, 175, 420, 663, 682, 726, 744
MroNI GCCGGC 1 cut(s) 261
MseI TTAA 2 cut(s) 516, 823
MspI CCGG 5 cut(s) 85, 256, 262, 268, 413
MspR9I CCNGG 5 cut(s) 256, 309, 414, 432, 794
MvaI CCWGG 3 cut(s) 309, 432, 794
MvnI CGCG 2 cut(s) 236, 329
MwoI GCNNNNNNNGC 5 cut(s) 30, 60, 250, 613, 692
NaeI GCCGGC 1 cut(s) 263
NarI GGCGCC 1 cut(s) 259
NciI CCSGG 2 cut(s) 256, 414
NdeII GATC 5 cut(s) 177, 200, 239, 499, 589
NgoMIV GCCGGC 1 cut(s) 261
NlaIII CATG 2 cut(s) 362, 406
NlaIV GGNNCC 4 cut(s) 145, 146, 260, 266
NmeAIII GCCGAG 1 cut(s) 778
NruI TCGCGA 1 cut(s) 236
NspI RCATGY 1 cut(s) 406
PceI AGGCCT 1 cut(s) 753
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PdiI GCCGGC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 430
PkrI GCNGC 7 cut(s) 32, 35, 62, 65, 191, 246, 694
PleI GAGTC 2 cut(s) 166, 540
PluTI GGCGCC 1 cut(s) 262
PpsI GAGTC 2 cut(s) 166, 540
PpuMI RGGWCCY 2 cut(s) 144, 731
Psp5II RGGWCCY 2 cut(s) 144, 731
Psp6I CCWGG 3 cut(s) 307, 430, 792
PspGI CCWGG 3 cut(s) 307, 430, 792
PspN4I GGNNCC 4 cut(s) 145, 146, 260, 266
PspPI GGNCC 3 cut(s) 4, 144, 731
PspPPI RGGWCCY 2 cut(s) 144, 731
PsuI RGATCY 1 cut(s) 177
RruI TCGCGA 1 cut(s) 236
SalI GTCGAC 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 516, 823
SatI GCNGC 7 cut(s) 31, 34, 61, 64, 190, 245, 693
Sau3AI GATC 5 cut(s) 177, 200, 239, 499, 589
Sau96I GGNCC 3 cut(s) 4, 144, 731
SchI GAGTC 2 cut(s) 166, 541
ScrFI CCNGG 5 cut(s) 256, 309, 414, 432, 794
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 2 cut(s) 622, 715
SfcI CTRYAG 2 cut(s) 19, 182
SfoI GGCGCC 1 cut(s) 260
SgrDI CGTCGACG 1 cut(s) 194
SinI GGWCC 3 cut(s) 4, 144, 731
Sse9I AATT 3 cut(s) 444, 525, 609
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 4 cut(s) 189, 251, 616, 720
SspDI GGCGCC 1 cut(s) 258
SspMI CTAG 1 cut(s) 656
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 5 cut(s) 254, 307, 412, 430, 792
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 3 cut(s) 223, 347, 454
TaiI ACGT 1 cut(s) 155
TaqI TCGA 5 cut(s) 170, 195, 203, 209, 273
TasI AATT 3 cut(s) 444, 525, 609
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 2 cut(s) 516, 823
Tru9I TTAA 2 cut(s) 516, 823
TseI GCWGC 6 cut(s) 30, 33, 60, 63, 244, 692
TspDTI ATGAA 2 cut(s) 318, 780
TspGWI ACGGA 4 cut(s) 117, 213, 220, 569
VpaK11BI GGWCC 3 cut(s) 4, 144, 731
XapI RAATTY 1 cut(s) 609
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 1 cut(s) 406
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.