FvH4_6g44480

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003521
Physical Location & Seq
Forward (+)
34261253 .. 34264990
3738 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44480.t2

Sequence Viewer

Length: 849 bp
ATGGCTGTGGAGGCTGAGAAATTTGGGTCAGACGGGAAGCTGTGGAATCTGTGCAAAAGGCCATTCTGGCATTCACGAAACGCTGCTCCTCCTCCTTATTCTTCGTCTTCTTCTTCGTCGTCCAATTTGTCTTCTATGCATAATGGTCACCAGCTTAGTCGGATTCACGAGTCTGTGGAGCGTCCGGCGCTTTATTCTGGCAGTACGGTGTCGTATGTGGCCAAGTCTCTGCTTCCCGCTAGGAGAAGGCTCCGTCTTGATCCTCCTAATAAGCTCTTCTTCCCATATGAACCTGGAAAGCAGGTTAAGAGTGCTGTTGGGATCAAAAACACTTCCAAGTCTCATGTTGCTTTCAAGTTCCAAACGACTGCACCAAAGAGTTGTTACATGCGTCCTCCAGGTGGAATACTTGCTCCTGGTGAAAGTCTTGTTGCAACTGTATTCAAGTTTGTGGAACCTCCGGAGAACAATGAAAAACCAGTAGACCAGAAAAGCAGGGTTAAGTTCAAGATCATGAGCTTGAAAGTTAAAGGAGAAATGGACTATGTTCCAGAACTGTTTGAGGAGCAGAAGGAGCAAGTGGTTGTTGAGCAGGTTTTACGTGTTGTTTTCCTTGACCCCGAACGCCCTGCCCTCGCAATGGAAAAACTCAAGCGGCAATTGGCTGAGGCCGAGGCTGCTCTTGACTCACGAAAGAAGCCTCCAGAAGAAGCGGGGCTGCGAATTGTAGGAGAAGGACTGGTCATAGATGAATGGAAAGAACGAAGGGAAAGATACCTAGCGCAGCAGCAGGTCGAAGGGGTTGTCGATTCAATGTTTAGAACTTCTGAAGGAAACCTACCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

283

Amino Acids

31.7

Weight (kDa)

9.34

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 84 - 193 5.5e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 292, 583, 781
AccI GTMKAC 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 3 cut(s) 237, 655, 713
AclWI GGATC 2 cut(s) 254, 329
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 1 cut(s) 205
AfiI CCNNNNNNNGG 2 cut(s) 401, 640
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 5 cut(s) 355, 445, 508, 523, 813
AjnI CCWGG 3 cut(s) 292, 397, 415
AluBI AGCT 4 cut(s) 40, 154, 274, 519
AluI AGCT 4 cut(s) 40, 154, 274, 519
Alw26I GTCTC 2 cut(s) 231, 345
AlwI GGATC 2 cut(s) 254, 329
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 3 cut(s) 59, 219, 669
ApeKI GCWGC 5 cut(s) 83, 677, 718, 784, 787
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 789, 821
AspLEI GCGC 2 cut(s) 190, 784
AsuHPI GGTGA 2 cut(s) 140, 431
BalI TGGCCA 1 cut(s) 221
BarI GAAGNNNNNNTAC 1 cut(s) 822
BauI CACGAG 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 99, 123
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 5 cut(s) 70, 664, 705, 796, 799
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 3 cut(s) 294, 399, 417
BcoDI GTCTC 2 cut(s) 231, 345
BfaI CTAG 2 cut(s) 240, 779
BfoI RGCGCY 1 cut(s) 191
BfuAI ACCTGC 3 cut(s) 292, 583, 781
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 6 cut(s) 84, 656, 678, 719, 785, 788
BlsI GCNGC 6 cut(s) 85, 657, 679, 720, 786, 789
Bme1390I CCNGG 3 cut(s) 294, 399, 417
BmiI GGNNCC 2 cut(s) 251, 456
BmrFI CCNGG 3 cut(s) 294, 399, 417
BpiI GAAGAC 2 cut(s) 99, 123
BpmI CTGGAG 2 cut(s) 381, 687
Bpu10I CCTNAGC 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 672
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 640
Bse1I ACTGG 2 cut(s) 479, 744
Bse3DI GCAATG 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 3 cut(s) 294, 399, 417
BseDI CCNNGG 1 cut(s) 672
BseLI CCNNNNNNNGG 2 cut(s) 401, 640
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 6, 657
BseNI ACTGG 2 cut(s) 479, 744
BseRI GAGGAG 3 cut(s) 78, 81, 578
BseXI GCAGC 5 cut(s) 70, 664, 705, 796, 799
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 3 cut(s) 61, 221, 671
BsiSI CCGG 2 cut(s) 185, 461
BslI CCNNNNNNNGG 2 cut(s) 401, 640
BsmAI GTCTC 2 cut(s) 231, 345
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 3 cut(s) 61, 221, 671
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 3 cut(s) 259, 321, 510
BspACI CCGC 3 cut(s) 237, 655, 713
BspANI GGCC 3 cut(s) 61, 221, 671
BspCNI CTCAG 2 cut(s) 7, 658
BspEI TCCGGA 1 cut(s) 460
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 2 cut(s) 251, 456
BspMI ACCTGC 3 cut(s) 292, 583, 781
BspPI GGATC 2 cut(s) 254, 329
BspQI GCTCTTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 2 cut(s) 479, 744
BssECI CCNNGG 1 cut(s) 672
BssMI GATC 3 cut(s) 259, 321, 510
BssSI CACGAG 1 cut(s) 167
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 3 cut(s) 294, 399, 417
Bst4CI ACNGT 3 cut(s) 208, 439, 558
Bst6I CTCTTC 1 cut(s) 281
BstBAI YACGTR 1 cut(s) 602
BstDEI CTNAG 3 cut(s) 15, 155, 666
BstEII GGTNACC 1 cut(s) 146
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 190, 784
BstKTI GATC 3 cut(s) 262, 324, 513
BstMAI GTCTC 2 cut(s) 231, 345
BstMBI GATC 3 cut(s) 259, 321, 510
BstMWI GCNNNNNNNGC 5 cut(s) 11, 67, 187, 574, 677
BstNI CCWGG 3 cut(s) 294, 399, 417
BstNSI RCATGY 1 cut(s) 391
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 3 cut(s) 292, 397, 415
BstV1I GCAGC 5 cut(s) 70, 664, 705, 796, 799
BstV2I GAAGAC 2 cut(s) 99, 123
BsuRI GGCC 3 cut(s) 61, 221, 671
BveI ACCTGC 3 cut(s) 292, 583, 781
CciI TCATGA 1 cut(s) 513
CfoI GCGC 2 cut(s) 190, 784
CseI GACGC 2 cut(s) 170, 380
Csp6I GTAC 1 cut(s) 204
CviAII CATG 3 cut(s) 344, 388, 514
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 3 cut(s) 15, 155, 666
DpnI GATC 3 cut(s) 261, 323, 512
DpnII GATC 3 cut(s) 259, 321, 510
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 849
Eco91I GGTNACC 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 292, 397, 415
EcoT22I ATGCAT 1 cut(s) 141
FaeI CATG 3 cut(s) 347, 391, 517
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 3 cut(s) 343, 387, 513
FauI CCCGC 2 cut(s) 244, 706
FauNDI CATATG 1 cut(s) 286
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 6 cut(s) 84, 656, 678, 719, 785, 788
Fsp4HI GCNGC 6 cut(s) 84, 656, 678, 719, 785, 788
FspBI CTAG 2 cut(s) 240, 779
GlaI GCGC 2 cut(s) 189, 783
GluI GCNGC 6 cut(s) 84, 656, 678, 719, 785, 788
GsuI CTGGAG 2 cut(s) 381, 687
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 3 cut(s) 61, 221, 671
HapII CCGG 2 cut(s) 185, 461
HgaI GACGC 2 cut(s) 170, 380
HhaI GCGC 2 cut(s) 190, 784
Hin1II CATG 3 cut(s) 347, 391, 517
Hin6I GCGC 2 cut(s) 188, 782
HinP1I GCGC 2 cut(s) 188, 782
HinfI GANTC 5 cut(s) 46, 163, 170, 686, 809
HpaII CCGG 2 cut(s) 185, 461
HphI GGTGA 2 cut(s) 140, 431
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 3 cut(s) 31, 162, 829
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 6 cut(s) 240, 565, 728, 759, 791, 824
HpyCH4III ACNGT 3 cut(s) 208, 439, 558
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 4 cut(s) 54, 139, 371, 434
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 67, 187, 574, 677
HpyF3I CTNAG 3 cut(s) 15, 155, 666
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 3 cut(s) 347, 391, 517
HspAI GCGC 2 cut(s) 188, 782
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 3 cut(s) 259, 321, 510
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 6 cut(s) 91, 178, 255, 418, 565, 574
Lsp1109I GCAGC 5 cut(s) 70, 664, 705, 796, 799
MaeI CTAG 2 cut(s) 240, 779
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 2 cut(s) 146, 383
MalI GATC 3 cut(s) 261, 323, 512
MboI GATC 3 cut(s) 259, 321, 510
MboII GAAGA 8 cut(s) 93, 99, 102, 105, 123, 268, 271, 719
MfeI CAATTG 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 4 cut(s) 20, 124, 659, 723
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 2 cut(s) 179, 680
MmeI TCCRAC 1 cut(s) 140
Mox20I TGGCCA 1 cut(s) 221
Mph1103I ATGCAT 1 cut(s) 141
MroI TCCGGA 1 cut(s) 460
MscI TGGCCA 1 cut(s) 221
MseI TTAA 4 cut(s) 306, 501, 528, 847
Msp20I TGGCCA 1 cut(s) 221
MspI CCGG 2 cut(s) 185, 461
MspR9I CCNGG 3 cut(s) 294, 399, 417
MunI CAATTG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 3 cut(s) 294, 399, 417
MwoI GCNNNNNNNGC 5 cut(s) 11, 67, 187, 574, 677
NdeI CATATG 1 cut(s) 286
NdeII GATC 3 cut(s) 259, 321, 510
NlaIII CATG 3 cut(s) 347, 391, 517
NlaIV GGNNCC 2 cut(s) 251, 456
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 146
NsiI ATGCAT 1 cut(s) 141
NspI RCATGY 1 cut(s) 391
PagI TCATGA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 281
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 3 cut(s) 46, 163, 809
PkrI GCNGC 6 cut(s) 85, 657, 679, 720, 786, 789
PleI GAGTC 2 cut(s) 178, 680
PpsI GAGTC 2 cut(s) 178, 680
Ppu21I YACGTR 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 292, 397, 415
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 3 cut(s) 292, 397, 415
PspN4I GGNNCC 2 cut(s) 251, 456
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 4 cut(s) 306, 501, 528, 847
SatI GCNGC 6 cut(s) 84, 656, 678, 719, 785, 788
Sau3AI GATC 3 cut(s) 259, 321, 510
SchI GAGTC 2 cut(s) 179, 680
ScrFI CCNGG 3 cut(s) 294, 399, 417
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 4 cut(s) 20, 124, 659, 723
SsiI CCGC 3 cut(s) 237, 655, 713
SspMI CTAG 2 cut(s) 240, 779
StyD4I CCNGG 3 cut(s) 292, 397, 415
TaaI ACNGT 3 cut(s) 208, 439, 558
TaiI ACGT 1 cut(s) 604
TaqI TCGA 2 cut(s) 795, 807
TasI AATT 4 cut(s) 20, 124, 659, 723
TauI GCSGC 1 cut(s) 658
TfiI GAWTC 3 cut(s) 46, 163, 809
Tru1I TTAA 4 cut(s) 306, 501, 528, 847
Tru9I TTAA 4 cut(s) 306, 501, 528, 847
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 5 cut(s) 83, 677, 718, 784, 787
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 3 cut(s) 303, 486, 765
TspGWI ACGGA 1 cut(s) 242
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 2 cut(s) 240, 779
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.