MD03G1217600.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003521
Physical Location & Seq
Forward (+)
30222191 .. 30224712
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1217600.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGCCATCGTCGACAGCCACCACCACCACCGGCACAAGTCGCACTCCGACACGAAGCTCTTCACCCTCTGCCCGTTCTGGCAGTCGGGGGGCCAAACGTCGTCGTCTTCCTCTTCCACGCAGAACCTCCACGGCGATGGATTCAAATCCAAGCCCAAAACGGTATCGTCCGTCGCGAGATCCCTTCTCCCTCCTCGTCGTCGACTCCGACTCGACCCCGCCAACAAACTCTACTTCCCTTATGAACCTGGGAAACAGGTGAAAAGTGCGATCCGATTGAAGAACAGTAGCAGGTCACATGTAGCTTTTAAGTTTCAAACTACTGCGCCGAAAAGTTGCTATATGCGTCCTCCGGGAGGAATACTTGCTCCGGGAGAAAGCTTAATTGCGACAGTGTTTAGGTTTGTGGAGCAACCCGAGAACAATGAGAAACATTTGGATCAGAAGAACAAGGTTAAGTTCAAGATTATGAGTCTCAAGGTGAACGCAGGGATCGACTATGTGCCTGAGCTGTTTGAGGAACAAAAGGATCAAGTGACAGTCGAGCGAATTTTGCGTGTTGTATTTTTGGATGCAGAGCACCCTAGTCCTGCTCTGGAAAAACTGAACAGACAATTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAGCCGCCTCCAGTGGACTCAGGCCCTCGGGATGTTGGGGAAGGTCTTGTAATAGATGAATGGAAGGAGCGGAGGGAGAAATACTTGGCTCGGCAACAGGTTGAAGCAGTAGATTCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

258

Amino Acids

29.05

Weight (kDa)

9.55

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 69 - 178 4.2e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 721
AccI GTMKAC 2 cut(s) 12, 202
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 219, 656, 721
AclWI GGATC 5 cut(s) 174, 265, 447, 500, 537
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 549
AcuI CTGAAG 1 cut(s) 642
AfiI CCNNNNNNNGG 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 6 cut(s) 145, 280, 317, 463, 641, 755
AjnI CCWGG 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AluBI AGCT 5 cut(s) 58, 305, 381, 511, 626
AluI AGCT 5 cut(s) 58, 305, 381, 511, 626
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 5 cut(s) 174, 265, 447, 500, 537
Ama87I CYCGRG 2 cut(s) 416, 678
AoxI GGCC 3 cut(s) 3, 91, 673
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 2 cut(s) 91, 674
AsuC2I CCSGG 2 cut(s) 354, 372
AsuHPI GGTGA 3 cut(s) 55, 271, 493
AvaI CYCGRG 2 cut(s) 416, 678
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 627
BbvI GCAGC 1 cut(s) 619
BccI CCATC 2 cut(s) 14, 131
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 249
BcnI CCSGG 2 cut(s) 354, 372
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 282
BisI GCNGC 2 cut(s) 633, 656
BlsI GCNGC 2 cut(s) 634, 657
Bme1390I CCNGG 3 cut(s) 249, 354, 372
BmeT110I CYCGRG 2 cut(s) 416, 678
BmgT120I GGNCC 2 cut(s) 91, 674
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 3 cut(s) 249, 354, 372
BmsI GCATC 1 cut(s) 562
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 645
Bpu10I CCTNAGC 2 cut(s) 507, 627
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 2 cut(s) 354, 372
BsaBI GATNNNNATC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 130, 248, 677
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 662
Bse8I GATNNNNATC 1 cut(s) 145
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 130, 248, 677
BseGI GGATG 2 cut(s) 577, 688
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 3 cut(s) 498, 618, 684
BseNI ACTGG 1 cut(s) 662
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 1 cut(s) 619
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 3 cut(s) 5, 93, 675
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 2 cut(s) 416, 678
BsiSI CCGG 3 cut(s) 31, 353, 371
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 479
BsnI GGCC 3 cut(s) 5, 93, 675
BsoBI CYCGRG 2 cut(s) 416, 678
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 5 cut(s) 179, 270, 439, 492, 529
Bsp68I TCGCGA 1 cut(s) 176
BspACI CCGC 3 cut(s) 219, 656, 721
BspANI GGCC 3 cut(s) 5, 93, 675
BspCNI CTCAG 3 cut(s) 499, 619, 683
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 92
BspMI ACCTGC 1 cut(s) 282
BspPI GGATC 5 cut(s) 174, 265, 447, 500, 537
BspQI GCTCTTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 721
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 1 cut(s) 662
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 3 cut(s) 130, 248, 677
BssMI GATC 5 cut(s) 179, 270, 439, 492, 529
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 4 cut(s) 163, 287, 394, 541
Bst6I CTCTTC 2 cut(s) 65, 118
BstDEI CTNAG 3 cut(s) 507, 627, 670
BstDSI CCRYGG 1 cut(s) 130
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 577, 688
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 5 cut(s) 182, 273, 442, 495, 532
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 5 cut(s) 179, 270, 439, 492, 529
BstMWI GCNNNNNNNGC 4 cut(s) 40, 553, 632, 652
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 3 cut(s) 247, 352, 370
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 619
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 179
BstXI CCANNNNNNTGG 1 cut(s) 137
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 3 cut(s) 5, 93, 675
BtgI CCRYGG 1 cut(s) 130
BtgZI GCGATG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 577, 688
BtsIMutI CAGTG 3 cut(s) 399, 669, 774
BtuMI TCGCGA 1 cut(s) 176
BveI ACCTGC 1 cut(s) 282
CfoI GCGC 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 91, 674
CseI GACGC 1 cut(s) 335
CviAII CATG 1 cut(s) 299
DdeI CTNAG 3 cut(s) 507, 627, 670
DpnI GATC 5 cut(s) 181, 272, 441, 494, 531
DpnII GATC 5 cut(s) 179, 270, 439, 492, 529
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 65, 118
EarI CTCTTC 2 cut(s) 65, 118
Eco57I CTGAAG 1 cut(s) 642
Eco88I CYCGRG 2 cut(s) 416, 678
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 1 cut(s) 302
FaiI YATR 6 cut(s) 243, 300, 342, 344, 470, 501
FatI CATG 1 cut(s) 298
FauI CCCGC 1 cut(s) 226
FblI GTMKAC 2 cut(s) 12, 202
Fnu4HI GCNGC 2 cut(s) 633, 656
FokI GGATG 2 cut(s) 584, 695
Fsp4HI GCNGC 2 cut(s) 633, 656
FspBI CTAG 1 cut(s) 585
GlaI GCGC 1 cut(s) 327
GluI GCNGC 2 cut(s) 633, 656
GsuI CTGGAG 1 cut(s) 645
HaeIII GGCC 3 cut(s) 5, 93, 675
HapII CCGG 3 cut(s) 31, 353, 371
HgaI GACGC 1 cut(s) 335
HhaI GCGC 1 cut(s) 328
Hin1II CATG 1 cut(s) 302
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 2 cut(s) 13, 203
HindII GTYRAC 2 cut(s) 13, 203
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 6 cut(s) 141, 204, 210, 472, 668, 764
HpaII CCGG 3 cut(s) 31, 353, 371
HphI GGTGA 3 cut(s) 55, 271, 493
Hpy166II GTNNAC 4 cut(s) 13, 203, 484, 667
Hpy188I TCNGA 4 cut(s) 49, 209, 275, 444
Hpy188III TCNNGA 4 cut(s) 175, 463, 596, 680
Hpy8I GTNNAC 4 cut(s) 13, 203, 484, 667
Hpy99I CGWCG 6 cut(s) 14, 103, 106, 176, 201, 204
HpyAV CCTTC 3 cut(s) 194, 686, 709
HpyCH4III ACNGT 4 cut(s) 163, 287, 394, 541
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 2 cut(s) 575, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 553, 632, 652
HpyF3I CTNAG 3 cut(s) 507, 627, 670
HpySE526I ACGT 1 cut(s) 98
Hsp92II CATG 1 cut(s) 302
HspAI GCGC 1 cut(s) 326
Kzo9I GATC 5 cut(s) 179, 270, 439, 492, 529
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 3 cut(s) 373, 409, 718
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 1 cut(s) 562
MaeI CTAG 1 cut(s) 585
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 294, 535
MalI GATC 5 cut(s) 181, 272, 441, 494, 531
MbiI CCGCTC 1 cut(s) 721
MboI GATC 5 cut(s) 179, 270, 439, 492, 529
MboII GAAGA 5 cut(s) 52, 99, 105, 292, 457
MfeI CAATTG 1 cut(s) 614
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 582
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 384, 549, 614
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 198, 204, 481, 662
MmeI TCCRAC 2 cut(s) 72, 232
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 309, 383, 456
MslI CAYNNNNRTG 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 31, 353, 371
MspR9I CCNGG 3 cut(s) 249, 354, 372
MunI CAATTG 1 cut(s) 614
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 4 cut(s) 40, 553, 632, 652
NciI CCSGG 2 cut(s) 354, 372
NdeII GATC 5 cut(s) 179, 270, 439, 492, 529
NlaIII CATG 1 cut(s) 302
NlaIV GGNNCC 1 cut(s) 92
NmeAIII GCCGAG 1 cut(s) 721
NmuCI GTSAC 2 cut(s) 294, 535
NruI TCGCGA 1 cut(s) 176
NspI RCATGY 1 cut(s) 302
PciI ACATGT 1 cut(s) 298
PciSI GCTCTTC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 3 cut(s) 173, 205, 492
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 141, 764
PfoI TCCNGGA 2 cut(s) 352, 370
PkrI GCNGC 2 cut(s) 634, 657
PleI GAGTC 4 cut(s) 198, 204, 480, 662
PpsI GAGTC 4 cut(s) 198, 204, 480, 662
PscI ACATGT 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 91, 674
PsuI RGATCY 1 cut(s) 179
RruI TCGCGA 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 135
SalI GTCGAC 2 cut(s) 11, 201
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 3 cut(s) 309, 383, 456
SatI GCNGC 2 cut(s) 633, 656
Sau3AI GATC 5 cut(s) 179, 270, 439, 492, 529
Sau96I GGNCC 2 cut(s) 91, 674
SchI GAGTC 4 cut(s) 198, 204, 481, 662
ScrFI CCNGG 3 cut(s) 249, 354, 372
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 3 cut(s) 384, 549, 614
SsiI CCGC 3 cut(s) 219, 656, 721
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 247, 352, 370
TaaI ACNGT 4 cut(s) 163, 287, 394, 541
TaiI ACGT 1 cut(s) 101
TaqI TCGA 5 cut(s) 12, 202, 213, 495, 543
TasI AATT 3 cut(s) 384, 549, 614
TauI GCSGC 1 cut(s) 658
TfiI GAWTC 2 cut(s) 141, 764
Tru1I TTAA 3 cut(s) 309, 383, 456
Tru9I TTAA 3 cut(s) 309, 383, 456
TscAI CASTG 3 cut(s) 399, 669, 774
TseFI GTSAC 2 cut(s) 294, 535
TseI GCWGC 1 cut(s) 632
Tsp45I GTSAC 2 cut(s) 294, 535
TspDTI ATGAA 2 cut(s) 258, 723
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 3 cut(s) 399, 669, 774
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 1 cut(s) 549
XceI RCATGY 1 cut(s) 302
XmiI GTMKAC 2 cut(s) 12, 202
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.