Rw2G046070

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003521
Physical Location & Seq
Forward (+)
73235896 .. 73239442
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046070.1

Sequence Viewer

Length: 819 bp
ATGGCTGTGGAGGCTGAGAAATTTGGGTCCGACGGGAAGCTTTGGAGTCTGTGCAAAAGGCCATTTTGGCAGTCAAGAAATGCTGCTCCTCCTTCTTACTCTTCATCTTCTTCTTCGTCTTCGAATTTGTCTTCTAGGCATAATGCTCAGCAGCTTAGTCGGATTCACGAGTCTGTGGAGCGTCCGACGCTTTATTCTGCCAGTACGGTGTCGTATGTGGCCAAGTCTTTGCTTCCCGCTAGGAGAAGGCTCCGTCTTGATCCTCCTAATAAGCTCTTCTTCCCATATGAACCTGGAAAGCAGGTTAAGAGTGCTGTTGGGATTAAAAACACTTCCAAGTCTCATGTAGCTTTCAAGTTCCAAACAACTGCACCAAAGAGTTGTTACATGCGTCCTCCTGGGGGAATACTTGCTCCTGGTGAAAGTCTTGTTGCAACTGTATTCAAGTTCGTGGAACCTCCGGAGAACAATGAAAAACCAGTAGACCAGAAAAGCAAGGTTAAGTTCAAGATCATGAGCTTAAAAGTGAAAGGAGAAATGGACTATGTTCCAGAATTGTTTGAGGAGCAGAAGGAACAAGTCGTTGTTGAGCAGGTTTTACGTGTTGTTTTCCTTGACCCGGAACGCCCTACCCTTGCAATGGAAAAACTCAAGCGGCAATTGGCTGAGGCCGAGGCTGCTCTTGAGTCACGAAAGAAGCCTCCAGAAGAAGCAGGGCCCCGAGTTGTAGGAGAAGGACTGGTCATAGATGAATGGAAAGAACGGAGGGAAAGATACCTAGCACAGCAGCAAGTTGAAGGAGTAGTTGATTCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

272

Amino Acids

30.62

Weight (kDa)

9.41

Isoelectric Point (pI)

56.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 84 - 193 4.4e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 292, 583
AccI GTMKAC 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 2 cut(s) 237, 655
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 2 cut(s) 20, 124
AfaI GTAC 1 cut(s) 205
AfiI CCNNNNNNNGG 3 cut(s) 401, 619, 640
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 5 cut(s) 355, 445, 508, 797, 813
AjnI CCWGG 3 cut(s) 292, 397, 415
AluBI AGCT 5 cut(s) 40, 154, 274, 350, 519
AluI AGCT 5 cut(s) 40, 154, 274, 350, 519
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 720
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 4 cut(s) 59, 219, 669, 716
ApaI GGGCCC 1 cut(s) 720
ApeKI GCWGC 4 cut(s) 83, 151, 677, 787
ApoI RAATTY 2 cut(s) 20, 124
AspS9I GGNCC 3 cut(s) 27, 716, 717
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 1 cut(s) 431
AsuII TTCGAA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 720
AvaII GGWCC 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 720
BalI TGGCCA 1 cut(s) 221
BanII GRGCYC 1 cut(s) 720
BauI CACGAG 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 111, 123
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 4 cut(s) 70, 163, 664, 799
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 3 cut(s) 294, 399, 417
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 3 cut(s) 135, 240, 779
BfuAI ACCTGC 2 cut(s) 292, 583
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 5 cut(s) 84, 152, 656, 678, 788
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 5 cut(s) 85, 153, 657, 679, 789
Bme1390I CCNGG 4 cut(s) 294, 399, 417, 620
Bme18I GGWCC 1 cut(s) 27
BmeT110I CYCGRG 1 cut(s) 720
BmgT120I GGNCC 3 cut(s) 27, 716, 717
BmiI GGNNCC 5 cut(s) 28, 251, 456, 718, 719
BmrFI CCNGG 4 cut(s) 294, 399, 417, 620
BpiI GAAGAC 2 cut(s) 111, 123
BpmI CTGGAG 1 cut(s) 687
Bpu10I CCTNAGC 1 cut(s) 666
Bpu1102I GCTNAGC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 2 cut(s) 635, 704
BpuMI CCSGG 1 cut(s) 620
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 398, 672
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 619, 640
Bse1I ACTGG 3 cut(s) 201, 479, 744
Bse3DI GCAATG 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 3 cut(s) 294, 399, 417
BseDI CCNNGG 2 cut(s) 398, 672
BseLI CCNNNNNNNGG 3 cut(s) 401, 619, 640
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 3 cut(s) 6, 161, 657
BseNI ACTGG 3 cut(s) 201, 479, 744
BseRI GAGGAG 2 cut(s) 78, 578
BseSI GKGCMC 1 cut(s) 720
BseXI GCAGC 4 cut(s) 70, 163, 664, 799
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 4 cut(s) 61, 221, 671, 718
BsiHKCI CYCGRG 1 cut(s) 720
BsiSI CCGG 2 cut(s) 461, 620
BslI CCNNNNNNNGG 3 cut(s) 401, 619, 640
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 4 cut(s) 61, 221, 671, 718
BsoBI CYCGRG 1 cut(s) 720
Bsp119I TTCGAA 1 cut(s) 122
Bsp120I GGGCCC 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 720
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 2 cut(s) 259, 510
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 2 cut(s) 237, 655
BspANI GGCC 4 cut(s) 61, 221, 671, 718
BspCNI CTCAG 3 cut(s) 7, 160, 658
BspEI TCCGGA 1 cut(s) 460
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 5 cut(s) 28, 251, 456, 718, 719
BspMI ACCTGC 2 cut(s) 292, 583
BspPI GGATC 1 cut(s) 254
BspQI GCTCTTC 1 cut(s) 281
BspT104I TTCGAA 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 3 cut(s) 201, 479, 744
BssECI CCNNGG 2 cut(s) 398, 672
BssMI GATC 2 cut(s) 259, 510
BssSI CACGAG 1 cut(s) 167
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 3 cut(s) 294, 399, 417
Bst4CI ACNGT 2 cut(s) 208, 439
Bst6I CTCTTC 2 cut(s) 106, 281
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 122
BstDEI CTNAG 4 cut(s) 15, 147, 155, 666
BstKTI GATC 2 cut(s) 262, 513
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 259, 510
BstMWI GCNNNNNNNGC 4 cut(s) 11, 67, 187, 677
BstNI CCWGG 3 cut(s) 294, 399, 417
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 4 cut(s) 292, 397, 415, 618
BstSLI GKGCMC 1 cut(s) 720
BstV1I GCAGC 4 cut(s) 70, 163, 664, 799
BstV2I GAAGAC 2 cut(s) 111, 123
BsuRI GGCC 4 cut(s) 61, 221, 671, 718
BveI ACCTGC 2 cut(s) 292, 583
CciI TCATGA 1 cut(s) 513
Cfr13I GGNCC 3 cut(s) 27, 716, 717
CseI GACGC 3 cut(s) 170, 196, 380
Csp6I GTAC 1 cut(s) 204
CviAII CATG 3 cut(s) 344, 388, 514
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 4 cut(s) 15, 147, 155, 666
DpnI GATC 2 cut(s) 261, 512
DpnII GATC 2 cut(s) 259, 510
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 106, 281
EarI CTCTTC 2 cut(s) 106, 281
Eco24I GRGCYC 1 cut(s) 720
Eco47I GGWCC 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 720
EcoO109I RGGNCCY 2 cut(s) 716, 717
EcoRII CCWGG 3 cut(s) 292, 397, 415
EcoT38I GRGCYC 1 cut(s) 720
FaeI CATG 3 cut(s) 347, 391, 517
FaiI YATR 9 cut(s) 141, 216, 286, 288, 345, 389, 515, 546, 746
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 3 cut(s) 343, 387, 513
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 1 cut(s) 286
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 5 cut(s) 84, 152, 656, 678, 788
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 5 cut(s) 84, 152, 656, 678, 788
FspBI CTAG 3 cut(s) 135, 240, 779
GluI GCNGC 5 cut(s) 84, 152, 656, 678, 788
GsuI CTGGAG 1 cut(s) 687
HaeIII GGCC 4 cut(s) 61, 221, 671, 718
HapII CCGG 2 cut(s) 461, 620
HgaI GACGC 3 cut(s) 170, 196, 380
Hin1II CATG 3 cut(s) 347, 391, 517
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 5 cut(s) 46, 163, 170, 686, 809
HpaII CCGG 2 cut(s) 461, 620
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 3 cut(s) 31, 162, 186
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 2 cut(s) 35, 190
HpyAV CCTTC 5 cut(s) 102, 240, 565, 728, 791
HpyCH4III ACNGT 2 cut(s) 208, 439
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 4 cut(s) 54, 371, 434, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 67, 187, 677
HpyF3I CTNAG 4 cut(s) 15, 147, 155, 666
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 3 cut(s) 347, 391, 517
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 259, 510
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 5 cut(s) 91, 178, 255, 418, 565
Lsp1109I GCAGC 4 cut(s) 70, 163, 664, 799
MaeI CTAG 3 cut(s) 135, 240, 779
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 2 cut(s) 383, 687
MalI GATC 2 cut(s) 261, 512
MboI GATC 2 cut(s) 259, 510
MboII GAAGA 9 cut(s) 93, 99, 102, 105, 111, 123, 268, 271, 719
MfeI CAATTG 1 cut(s) 659
MhlI GDGCHC 1 cut(s) 720
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 4 cut(s) 20, 124, 554, 659
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 3 cut(s) 55, 179, 695
MmeI TCCRAC 3 cut(s) 54, 140, 209
Mox20I TGGCCA 1 cut(s) 221
MroI TCCGGA 1 cut(s) 460
MscI TGGCCA 1 cut(s) 221
MseI TTAA 4 cut(s) 306, 324, 501, 521
Msp20I TGGCCA 1 cut(s) 221
MspI CCGG 2 cut(s) 461, 620
MspR9I CCNGG 4 cut(s) 294, 399, 417, 620
MunI CAATTG 1 cut(s) 659
MvaI CCWGG 3 cut(s) 294, 399, 417
MwoI GCNNNNNNNGC 4 cut(s) 11, 67, 187, 677
NciI CCSGG 1 cut(s) 620
NdeI CATATG 1 cut(s) 286
NdeII GATC 2 cut(s) 259, 510
NlaIII CATG 3 cut(s) 347, 391, 517
NlaIV GGNNCC 5 cut(s) 28, 251, 456, 718, 719
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 687
NspI RCATGY 1 cut(s) 391
NspV TTCGAA 1 cut(s) 122
PagI TCATGA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 163, 809
PkrI GCNGC 5 cut(s) 85, 153, 657, 679, 789
PleI GAGTC 3 cut(s) 54, 178, 694
PpsI GAGTC 3 cut(s) 54, 178, 694
Ppu21I YACGTR 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 292, 397, 415
PspGI CCWGG 3 cut(s) 292, 397, 415
PspN4I GGNNCC 5 cut(s) 28, 251, 456, 718, 719
PspOMI GGGCCC 1 cut(s) 716
PspPI GGNCC 3 cut(s) 27, 716, 717
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 4 cut(s) 306, 324, 501, 521
SatI GCNGC 5 cut(s) 84, 152, 656, 678, 788
Sau3AI GATC 2 cut(s) 259, 510
Sau96I GGNCC 3 cut(s) 27, 716, 717
SchI GAGTC 3 cut(s) 55, 179, 695
ScrFI CCNGG 4 cut(s) 294, 399, 417, 620
SduI GDGCHC 1 cut(s) 720
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 650, 683
SmoI CTYRAG 2 cut(s) 650, 683
Sse9I AATT 4 cut(s) 20, 124, 554, 659
SsiI CCGC 2 cut(s) 237, 655
SspMI CTAG 3 cut(s) 135, 240, 779
StyD4I CCNGG 4 cut(s) 292, 397, 415, 618
TaaI ACNGT 2 cut(s) 208, 439
TaiI ACGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 122
TasI AATT 4 cut(s) 20, 124, 554, 659
TauI GCSGC 1 cut(s) 658
TfiI GAWTC 2 cut(s) 163, 809
Tru1I TTAA 4 cut(s) 306, 324, 501, 521
Tru9I TTAA 4 cut(s) 306, 324, 501, 521
TseFI GTSAC 1 cut(s) 687
TseI GCWGC 4 cut(s) 83, 151, 677, 787
Tsp45I GTSAC 1 cut(s) 687
TspDTI ATGAA 4 cut(s) 93, 303, 486, 765
TspGWI ACGGA 2 cut(s) 242, 778
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 2 cut(s) 20, 124
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 3 cut(s) 135, 240, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.