RLG00000033673

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003521
Physical Location & Seq
Reverse (-)
30581311 .. 30583206
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033673

Sequence Viewer

Length: 978 bp
ATGTCCTCCACACCCTGGCAACTCACAAGTTGCAACTACCCTGAAGAAAATTCTGAGAAAGAAGAAGAAGAAGAAGAGAAATTCAATCACCGAGTTTGTTATAGAGCTCGATTTCAATGGTGTAAAGCCGCCACGTGTCAGATTCCGGCGAAGAATATAATCATGGACCACCACCACCACGACTACAGCCACTCCCGCCACCACCACCGCCACCACAGCCAGAGCAGCAGCCACCACCGGCACAAGTCCCACTCCGACGGCAACCTCTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGCGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCAGTCGCGAGATCGCTGCTCCCGCCTCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTCTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCAATTCGGTTGAAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCTGACAGCAATGATAAAAATGTGGATCAGAAGACCAAGGTTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGATCAAGTAGCAGTTGAGCAAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTTTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTCGAAGTGCGTAAGAAACCTCCCGCGGATTCTGGTCCTCGTGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTTGAAGCAGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

326

Amino Acids

37.01

Weight (kDa)

9.14

Isoelectric Point (pI)

58.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 140 - 250 1.7e-24 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 15
AccBSI CCGCTC 1 cut(s) 342
AccI GTMKAC 1 cut(s) 348
AccII CGCG 2 cut(s) 389, 875
AciI CCGC 8 cut(s) 129, 196, 208, 342, 404, 769, 873, 875
AclWI GGATC 4 cut(s) 325, 361, 660, 750
AcsI RAATTY 3 cut(s) 49, 80, 762
AcuI CTGAAG 4 cut(s) 63, 710, 839, 855
AcvI CACGTG 1 cut(s) 135
AcyI GRCGYC 2 cut(s) 345, 412
AfiI CCNNNNNNNGG 2 cut(s) 15, 569
AflIII ACRYGT 1 cut(s) 134
AgsI TTSAA 7 cut(s) 85, 116, 493, 523, 530, 676, 965
AjnI CCWGG 4 cut(s) 14, 460, 583, 945
AjuI GAANNNNNNNTTGG 4 cut(s) 656, 688, 812, 844
AluBI AGCT 6 cut(s) 107, 518, 549, 701, 724, 839
AluI AGCT 6 cut(s) 107, 518, 549, 701, 724, 839
Alw21I GWGCWC 2 cut(s) 109, 795
Alw26I GTCTC 1 cut(s) 513
AlwI GGATC 4 cut(s) 325, 361, 660, 750
AoxI GGCC 1 cut(s) 904
ApeKI GCWGC 4 cut(s) 225, 228, 397, 845
ApoI RAATTY 3 cut(s) 49, 80, 762
AspLEI GCGC 2 cut(s) 414, 482
AspS9I GGNCC 3 cut(s) 166, 297, 884
AsuC2I CCSGG 1 cut(s) 567
AsuHPI GGTGA 2 cut(s) 80, 706
AvaII GGWCC 3 cut(s) 166, 297, 884
BanI GGYRCC 1 cut(s) 411
BanII GRGCYC 1 cut(s) 109
BauI CACGAG 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 135
BbsI GAAGAC 2 cut(s) 306, 665
Bbv12I GWGCWC 2 cut(s) 109, 795
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 4 cut(s) 237, 240, 384, 832
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 379, 413
BciT130I CCWGG 4 cut(s) 16, 462, 585, 947
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 513
BfmI CTRYAG 2 cut(s) 184, 335
BfoI RGCGCY 1 cut(s) 415
BisI GCNGC 6 cut(s) 129, 226, 229, 343, 398, 846
BlsI GCNGC 6 cut(s) 130, 227, 230, 344, 399, 847
Bme1390I CCNGG 5 cut(s) 16, 462, 567, 585, 947
Bme18I GGWCC 3 cut(s) 166, 297, 884
BmgT120I GGNCC 3 cut(s) 166, 297, 884
BmiI GGNNCC 4 cut(s) 298, 299, 413, 419
BmrFI CCNGG 5 cut(s) 16, 462, 567, 585, 947
BmsI GCATC 1 cut(s) 775
BpiI GAAGAC 2 cut(s) 306, 665
BpmI CTGGAG 1 cut(s) 567
Bpu10I CCTNAGC 2 cut(s) 720, 840
BpuMI CCSGG 1 cut(s) 567
BsaAI YACGTR 1 cut(s) 135
BsaHI GRCGYC 2 cut(s) 345, 412
BsaJI CCNNGG 5 cut(s) 14, 407, 461, 663, 873
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 569
Bse118I RCCGGY 2 cut(s) 237, 414
Bse3DI GCAATG 1 cut(s) 643
BseBI CCWGG 4 cut(s) 16, 462, 585, 947
BseDI CCNNGG 5 cut(s) 14, 407, 461, 663, 873
BseLI CCNNNNNNNGG 2 cut(s) 15, 569
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 4 cut(s) 45, 341, 711, 831
BseXI GCAGC 4 cut(s) 237, 240, 384, 832
BsgI GTGCAG 2 cut(s) 522, 831
Bsh1236I CGCG 2 cut(s) 389, 875
BshFI GGCC 1 cut(s) 906
BshNI GGYRCC 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 109, 795
BsiSI CCGG 5 cut(s) 146, 238, 415, 421, 566
BslFI GGGAC 2 cut(s) 232, 283
BslI CCNNNNNNNGG 2 cut(s) 15, 569
BsmAI GTCTC 1 cut(s) 513
BsmFI GGGAC 2 cut(s) 232, 283
BsnI GGCC 1 cut(s) 906
Bsp1286I GDGCHC 2 cut(s) 109, 795
Bsp143I GATC 5 cut(s) 330, 353, 392, 652, 742
Bsp68I TCGCGA 1 cut(s) 389
BspACI CCGC 8 cut(s) 129, 196, 208, 342, 404, 769, 873, 875
BspANI GGCC 1 cut(s) 906
BspCNI CTCAG 4 cut(s) 46, 340, 712, 832
BspFNI CGCG 2 cut(s) 389, 875
BspLI GGNNCC 4 cut(s) 298, 299, 413, 419
BspPI GGATC 4 cut(s) 325, 361, 660, 750
BspQI GCTCTTC 1 cut(s) 470
BspT107I GGYRCC 1 cut(s) 411
BsrBI CCGCTC 1 cut(s) 342
BsrDI GCAATG 1 cut(s) 643
BsrFI RCCGGY 2 cut(s) 237, 414
BssAI RCCGGY 2 cut(s) 237, 414
BssECI CCNNGG 5 cut(s) 14, 407, 461, 663, 873
BssMI GATC 5 cut(s) 330, 353, 392, 652, 742
BssNI GRCGYC 2 cut(s) 345, 412
BssSI CACGAG 1 cut(s) 888
BssT1I CCWWGG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 4 cut(s) 16, 462, 585, 947
Bst4CI ACNGT 3 cut(s) 376, 500, 607
Bst6I CTCTTC 5 cut(s) 69, 272, 325, 470, 815
BstACI GRCGYC 2 cut(s) 345, 412
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 416, 436
BstDEI CTNAG 4 cut(s) 54, 327, 720, 840
BstDSI CCRYGG 1 cut(s) 873
BstFNI CGCG 2 cut(s) 389, 875
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 414, 482
BstKTI GATC 5 cut(s) 333, 356, 395, 655, 745
BstMAI GTCTC 1 cut(s) 513
BstMBI GATC 5 cut(s) 330, 353, 392, 652, 742
BstMWI GCNNNNNNNGC 6 cut(s) 195, 216, 225, 403, 766, 845
BstNI CCWGG 4 cut(s) 16, 462, 585, 947
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 5 cut(s) 14, 460, 565, 583, 945
BstSFI CTRYAG 2 cut(s) 184, 335
BstUI CGCG 2 cut(s) 389, 875
BstV1I GCAGC 4 cut(s) 237, 240, 384, 832
BstV2I GAAGAC 2 cut(s) 306, 665
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 1 cut(s) 906
BtgI CCRYGG 1 cut(s) 873
BtuMI TCGCGA 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 416, 436
CfoI GCGC 2 cut(s) 414, 482
Cfr10I RCCGGY 2 cut(s) 237, 414
Cfr13I GGNCC 3 cut(s) 166, 297, 884
Cfr42I CCGCGG 1 cut(s) 876
CseI GACGC 2 cut(s) 334, 548
CviAII CATG 3 cut(s) 163, 512, 556
DdeI CTNAG 4 cut(s) 54, 327, 720, 840
DinI GGCGCC 1 cut(s) 413
DpnI GATC 5 cut(s) 332, 355, 394, 654, 744
DpnII GATC 5 cut(s) 330, 353, 392, 652, 742
Eam1104I CTCTTC 5 cut(s) 69, 272, 325, 470, 815
EarI CTCTTC 5 cut(s) 69, 272, 325, 470, 815
Ecl136II GAGCTC 1 cut(s) 107
Eco130I CCWWGG 1 cut(s) 663
Eco147I AGGCCT 1 cut(s) 906
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 3 cut(s) 166, 297, 884
Eco53kI GAGCTC 1 cut(s) 107
Eco57I CTGAAG 4 cut(s) 63, 710, 839, 855
Eco72I CACGTG 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 297
EcoRII CCWGG 4 cut(s) 14, 460, 583, 945
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 109
EgeI GGCGCC 1 cut(s) 413
EheI GGCGCC 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 3 cut(s) 166, 515, 559
FaiI YATR 9 cut(s) 102, 158, 164, 456, 513, 557, 596, 683, 714
FaqI GGGAC 2 cut(s) 232, 283
FatI CATG 3 cut(s) 162, 511, 555
FauI CCCGC 4 cut(s) 203, 411, 762, 880
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 6 cut(s) 129, 226, 229, 343, 398, 846
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 6 cut(s) 129, 226, 229, 343, 398, 846
GlaI GCGC 2 cut(s) 413, 481
GluI GCNGC 6 cut(s) 129, 226, 229, 343, 398, 846
GsuI CTGGAG 1 cut(s) 567
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 1 cut(s) 906
HapII CCGG 5 cut(s) 146, 238, 415, 421, 566
HgaI GACGC 2 cut(s) 334, 548
HhaI GCGC 2 cut(s) 414, 482
Hin1I GRCGYC 2 cut(s) 345, 412
Hin1II CATG 3 cut(s) 166, 515, 559
Hin6I GCGC 2 cut(s) 412, 480
HinP1I GCGC 2 cut(s) 412, 480
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 4 cut(s) 142, 325, 685, 878
HpaII CCGG 5 cut(s) 146, 238, 415, 421, 566
HphI GGTGA 2 cut(s) 80, 706
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 6 cut(s) 55, 141, 256, 330, 657, 690
Hpy188III TCNNGA 3 cut(s) 388, 719, 890
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 5 cut(s) 260, 310, 313, 350, 353
HpyAV CCTTC 4 cut(s) 685, 896, 919, 927
HpyCH4III ACNGT 3 cut(s) 376, 500, 607
HpyCH4IV ACGT 2 cut(s) 134, 305
HpyCH4V TGCA 4 cut(s) 33, 539, 788, 848
HpyF10VI GCNNNNNNNGC 6 cut(s) 195, 216, 225, 403, 766, 845
HpyF3I CTNAG 4 cut(s) 54, 327, 720, 840
HpySE526I ACGT 2 cut(s) 134, 305
Hsp92I GRCGYC 2 cut(s) 345, 412
Hsp92II CATG 3 cut(s) 166, 515, 559
HspAI GCGC 2 cut(s) 412, 480
KasI GGCGCC 1 cut(s) 411
KflI GGGWCCC 1 cut(s) 297
KroI GCCGGC 1 cut(s) 414
KroNI GCCGGC 1 cut(s) 416
KspI CCGCGG 1 cut(s) 876
Kzo9I GATC 5 cut(s) 330, 353, 392, 652, 742
LguI GCTCTTC 1 cut(s) 470
LmnI GCTCC 5 cut(s) 405, 423, 586, 721, 928
Lsp1109I GCAGC 4 cut(s) 237, 240, 384, 832
LweI GCATC 1 cut(s) 775
MaeII ACGT 2 cut(s) 134, 305
MaeIII GTNAC 1 cut(s) 551
MalI GATC 5 cut(s) 332, 355, 394, 654, 744
MbiI CCGCTC 1 cut(s) 342
MboI GATC 5 cut(s) 330, 353, 392, 652, 742
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 2 cut(s) 109, 795
MluCI AATT 6 cut(s) 49, 80, 483, 597, 678, 762
Mly113I GGCGCC 1 cut(s) 412
MlyI GAGTC 2 cut(s) 319, 694
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 9 cut(s) 16, 275, 328, 417, 573, 816, 835, 879, 897
MroNI GCCGGC 1 cut(s) 414
MseI TTAA 2 cut(s) 669, 976
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 5 cut(s) 146, 238, 415, 421, 566
MspR9I CCNGG 5 cut(s) 16, 462, 567, 585, 947
MvaI CCWGG 4 cut(s) 16, 462, 585, 947
MvnI CGCG 2 cut(s) 389, 875
MwoI GCNNNNNNNGC 6 cut(s) 195, 216, 225, 403, 766, 845
NaeI GCCGGC 1 cut(s) 416
NarI GGCGCC 1 cut(s) 412
NciI CCSGG 1 cut(s) 567
NdeII GATC 5 cut(s) 330, 353, 392, 652, 742
NgoMIV GCCGGC 1 cut(s) 414
NlaIII CATG 3 cut(s) 166, 515, 559
NlaIV GGNNCC 4 cut(s) 298, 299, 413, 419
NmeAIII GCCGAG 2 cut(s) 388, 931
NruI TCGCGA 1 cut(s) 389
NspI RCATGY 1 cut(s) 559
PceI AGGCCT 1 cut(s) 906
PciSI GCTCTTC 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 386
PdiI GCCGGC 1 cut(s) 416
PfeI GAWTC 2 cut(s) 142, 878
PflMI CCANNNNNTGG 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 583
PkrI GCNGC 6 cut(s) 130, 227, 230, 344, 399, 847
PleI GAGTC 2 cut(s) 319, 693
PluTI GGCGCC 1 cut(s) 415
PmaCI CACGTG 1 cut(s) 135
PmlI CACGTG 1 cut(s) 135
PpsI GAGTC 2 cut(s) 319, 693
Ppu21I YACGTR 1 cut(s) 135
PpuMI RGGWCCY 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 297
Psp6I CCWGG 4 cut(s) 14, 460, 583, 945
PspCI CACGTG 1 cut(s) 135
PspGI CCWGG 4 cut(s) 14, 460, 583, 945
PspN4I GGNNCC 4 cut(s) 298, 299, 413, 419
PspPI GGNCC 3 cut(s) 166, 297, 884
PspPPI RGGWCCY 1 cut(s) 297
PsuI RGATCY 1 cut(s) 330
RruI TCGCGA 1 cut(s) 389
SacI GAGCTC 1 cut(s) 109
SacII CCGCGG 1 cut(s) 876
SalI GTCGAC 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 470
SaqAI TTAA 2 cut(s) 669, 976
SatI GCNGC 6 cut(s) 129, 226, 229, 343, 398, 846
Sau3AI GATC 5 cut(s) 330, 353, 392, 652, 742
Sau96I GGNCC 3 cut(s) 166, 297, 884
SchI GAGTC 2 cut(s) 319, 694
ScrFI CCNGG 5 cut(s) 16, 462, 567, 585, 947
SduI GDGCHC 2 cut(s) 109, 795
SfaNI GCATC 1 cut(s) 775
SfcI CTRYAG 2 cut(s) 184, 335
SfoI GGCGCC 1 cut(s) 413
Sfr303I CCGCGG 1 cut(s) 876
SgrBI CCGCGG 1 cut(s) 876
SgrDI CGTCGACG 1 cut(s) 347
SinI GGWCC 3 cut(s) 166, 297, 884
Sse9I AATT 6 cut(s) 49, 80, 483, 597, 678, 762
SseBI AGGCCT 1 cut(s) 906
SsiI CCGC 8 cut(s) 129, 196, 208, 342, 404, 769, 873, 875
SspDI GGCGCC 1 cut(s) 411
SstI GAGCTC 1 cut(s) 109
StuI AGGCCT 1 cut(s) 906
StyD4I CCNGG 5 cut(s) 14, 460, 565, 583, 945
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 3 cut(s) 376, 500, 607
TaiI ACGT 2 cut(s) 137, 308
TaqI TCGA 7 cut(s) 109, 323, 348, 356, 362, 426, 852
TasI AATT 6 cut(s) 49, 80, 483, 597, 678, 762
TauI GCSGC 2 cut(s) 131, 345
TfiI GAWTC 2 cut(s) 142, 878
Tru1I TTAA 2 cut(s) 669, 976
Tru9I TTAA 2 cut(s) 669, 976
TseI GCWGC 4 cut(s) 225, 228, 397, 845
TspDTI ATGAA 2 cut(s) 471, 933
TspGWI ACGGA 3 cut(s) 270, 366, 722
Van91I CCANNNNNTGG 1 cut(s) 15
VpaK11BI GGWCC 3 cut(s) 166, 297, 884
XapI RAATTY 3 cut(s) 49, 80, 762
XceI RCATGY 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.