Rroxscaffold_1G00044610

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003521
Physical Location & Seq
Forward (+)
63613547 .. 63615882
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044610.1

Sequence Viewer

Length: 1155 bp
ATGTCCTTCACACCCTGGCAACCCACAAGTTGCAACCACCCTGAAGAAAATTCTGAGAGAGAAGAAGAAGAAGAAGGAGAAGAGAAATTCAATCACCGAGTTTGTTATAGAGCTCGATTTCAATGGTGTAAAGCCGCCACGTGTCAGATTCCGGCGAAGAATTCAAGCATGGACCAACACCGCCAGCACCACCACGACTACAGCCACTCCCACCACCACCACCGCCACCACAGCCAGAGCAGCAGCCACCACCATCACCACCGGCACAAGTCCCACTCCGACGGCAACCTGTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGCGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTGCTCCCGCCCCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTCTACTTCCCCTGTACGTCGTCGTTTCTCAAGTTCAACGCCTCCGTTGACCTTTATGAACCTGGGAAACAAGTGAAGAGCGCGATTCGGTTGAAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCTGACAGCAATGAGAAAAATGTGGATCAGAAGACCAAGGTTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACTGACTATGCTCCTGAGCTGGTAAATATAATGCTCTTCCCTCTTTATGCTCTCTGCGGCTTGTTAATTGTCCGTGCATACACTTTCGGGAAATTTGACTGCCAGTTTCCAATTGATCCTTTGTTGAGACAGTTTGACGAGCAAAAGGATCAAGTAGCAGTTGAGCGAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTCTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTCGAAGTGCGTAAGAAACCTCCCGCAGATTCAGGTCCTCGTGATGTTGGAGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTTGAAGCAGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

384

Amino Acids

43.82

Weight (kDa)

9.07

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 167 - 264 2.2e-19 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 435
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 1 cut(s) 372
AccII CGCG 2 cut(s) 413, 548
AciI CCGC 8 cut(s) 135, 181, 223, 366, 428, 828, 946, 1050
AclWI GGATC 5 cut(s) 349, 385, 726, 881, 927
AcsI RAATTY 5 cut(s) 49, 86, 160, 863, 939
AcuI CTGAAG 4 cut(s) 63, 776, 1016, 1032
AcvI CACGTG 1 cut(s) 141
AcyI GRCGYC 2 cut(s) 369, 436
AfaI GTAC 1 cut(s) 481
AfiI CCNNNNNNNGG 2 cut(s) 438, 635
AflIII ACRYGT 1 cut(s) 140
AgsI TTSAA 9 cut(s) 91, 122, 165, 502, 559, 589, 596, 742, 1142
AjnI CCWGG 4 cut(s) 14, 526, 649, 1122
AjuI GAANNNNNNNTTGG 4 cut(s) 722, 754, 989, 1021
AluBI AGCT 6 cut(s) 113, 584, 615, 767, 790, 1016
AluI AGCT 6 cut(s) 113, 584, 615, 767, 790, 1016
Alw21I GWGCWC 2 cut(s) 115, 972
Alw26I GTCTC 2 cut(s) 579, 892
AlwI GGATC 5 cut(s) 349, 385, 726, 881, 927
AoxI GGCC 1 cut(s) 1081
ApeKI GCWGC 4 cut(s) 240, 243, 421, 1022
ApoI RAATTY 5 cut(s) 49, 86, 160, 863, 939
AspLEI GCGC 2 cut(s) 438, 548
AspS9I GGNCC 3 cut(s) 172, 321, 1061
AsuC2I CCSGG 2 cut(s) 433, 633
AsuHPI GGTGA 3 cut(s) 86, 248, 772
AvaII GGWCC 3 cut(s) 172, 321, 1061
BanI GGYRCC 1 cut(s) 435
BanII GRGCYC 1 cut(s) 115
BauI CACGAG 1 cut(s) 1065
BbrPI CACGTG 1 cut(s) 141
BbsI GAAGAC 2 cut(s) 330, 731
Bbv12I GWGCWC 2 cut(s) 115, 972
BbvCI CCTCAGC 1 cut(s) 1017
BbvI GCAGC 4 cut(s) 252, 255, 408, 1009
BccI CCATC 1 cut(s) 261
BceAI ACGGC 1 cut(s) 298
BcgI CGANNNNNNTGC 2 cut(s) 403, 437
BciT130I CCWGG 4 cut(s) 16, 528, 651, 1124
BcnI CCSGG 2 cut(s) 433, 633
BcoDI GTCTC 2 cut(s) 579, 892
BfaI CTAG 1 cut(s) 986
BfmI CTRYAG 2 cut(s) 199, 359
BfoI RGCGCY 1 cut(s) 439
BisI GCNGC 7 cut(s) 135, 241, 244, 367, 422, 829, 1023
BlsI GCNGC 7 cut(s) 136, 242, 245, 368, 423, 830, 1024
Bme1390I CCNGG 6 cut(s) 16, 433, 528, 633, 651, 1124
Bme18I GGWCC 3 cut(s) 172, 321, 1061
BmgT120I GGNCC 3 cut(s) 172, 321, 1061
BmiI GGNNCC 4 cut(s) 322, 323, 437, 443
BmrFI CCNGG 6 cut(s) 16, 433, 528, 633, 651, 1124
BmsI GCATC 1 cut(s) 952
BpiI GAAGAC 2 cut(s) 330, 731
BpmI CTGGAG 1 cut(s) 633
Bpu10I CCTNAGC 2 cut(s) 786, 1017
BpuEI CTTGAG 1 cut(s) 479
BpuMI CCSGG 2 cut(s) 433, 633
BsaAI YACGTR 1 cut(s) 141
BsaHI GRCGYC 2 cut(s) 369, 436
BsaJI CCNNGG 4 cut(s) 14, 431, 527, 729
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 635
Bse118I RCCGGY 2 cut(s) 261, 438
Bse1I ACTGG 1 cut(s) 874
Bse3DI GCAATG 1 cut(s) 709
BseBI CCWGG 4 cut(s) 16, 528, 651, 1124
BseDI CCNNGG 4 cut(s) 14, 431, 527, 729
BseLI CCNNNNNNNGG 2 cut(s) 438, 635
BseMI GCAATG 1 cut(s) 709
BseMII CTCAG 4 cut(s) 45, 365, 777, 1008
BseNI ACTGG 1 cut(s) 874
BseXI GCAGC 4 cut(s) 252, 255, 408, 1009
BsgI GTGCAG 2 cut(s) 588, 1008
Bsh1236I CGCG 2 cut(s) 413, 548
BshFI GGCC 1 cut(s) 1083
BshNI GGYRCC 1 cut(s) 435
BsiHKAI GWGCWC 2 cut(s) 115, 972
BsiSI CCGG 6 cut(s) 152, 262, 433, 439, 445, 632
BslFI GGGAC 2 cut(s) 256, 307
BslI CCNNNNNNNGG 2 cut(s) 438, 635
BsmAI GTCTC 2 cut(s) 579, 892
BsmFI GGGAC 2 cut(s) 256, 307
BsnI GGCC 1 cut(s) 1083
Bsp1286I GDGCHC 2 cut(s) 115, 972
Bsp143I GATC 6 cut(s) 354, 377, 416, 718, 886, 919
Bsp68I TCGCGA 1 cut(s) 413
BspACI CCGC 8 cut(s) 135, 181, 223, 366, 428, 828, 946, 1050
BspANI GGCC 1 cut(s) 1083
BspCNI CTCAG 4 cut(s) 46, 364, 778, 1009
BspFNI CGCG 2 cut(s) 413, 548
BspLI GGNNCC 4 cut(s) 322, 323, 437, 443
BspPI GGATC 5 cut(s) 349, 385, 726, 881, 927
BspQI GCTCTTC 2 cut(s) 536, 812
BspT107I GGYRCC 1 cut(s) 435
BsrBI CCGCTC 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 709
BsrFI RCCGGY 2 cut(s) 261, 438
BsrI ACTGG 1 cut(s) 874
BssAI RCCGGY 2 cut(s) 261, 438
BssECI CCNNGG 4 cut(s) 14, 431, 527, 729
BssMI GATC 6 cut(s) 354, 377, 416, 718, 886, 919
BssNI GRCGYC 2 cut(s) 369, 436
BssSI CACGAG 1 cut(s) 1065
BssT1I CCWWGG 1 cut(s) 729
Bst2BI CACGAG 1 cut(s) 1065
Bst2UI CCWGG 4 cut(s) 16, 528, 651, 1124
Bst4CI ACNGT 4 cut(s) 400, 566, 673, 903
Bst6I CTCTTC 5 cut(s) 75, 349, 536, 812, 992
BstACI GRCGYC 2 cut(s) 369, 436
BstBAI YACGTR 1 cut(s) 141
BstC8I GCNNGC 3 cut(s) 185, 440, 460
BstDEI CTNAG 4 cut(s) 54, 351, 786, 1017
BstFNI CGCG 2 cut(s) 413, 548
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 2 cut(s) 438, 548
BstKTI GATC 6 cut(s) 357, 380, 419, 721, 889, 922
BstMAI GTCTC 2 cut(s) 579, 892
BstMBI GATC 6 cut(s) 354, 377, 416, 718, 886, 919
BstMWI GCNNNNNNNGC 5 cut(s) 231, 240, 427, 943, 1022
BstNI CCWGG 4 cut(s) 16, 528, 651, 1124
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 6 cut(s) 14, 431, 526, 631, 649, 1122
BstSFI CTRYAG 2 cut(s) 199, 359
BstUI CGCG 2 cut(s) 413, 548
BstV1I GCAGC 4 cut(s) 252, 255, 408, 1009
BstV2I GAAGAC 2 cut(s) 330, 731
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuRI GGCC 1 cut(s) 1083
BtuMI TCGCGA 1 cut(s) 413
Cac8I GCNNGC 3 cut(s) 185, 440, 460
CfoI GCGC 2 cut(s) 438, 548
Cfr10I RCCGGY 2 cut(s) 261, 438
Cfr13I GGNCC 3 cut(s) 172, 321, 1061
CseI GACGC 2 cut(s) 358, 614
Csp6I GTAC 1 cut(s) 480
CviAII CATG 3 cut(s) 169, 578, 622
CviQI GTAC 1 cut(s) 480
DdeI CTNAG 4 cut(s) 54, 351, 786, 1017
DinI GGCGCC 1 cut(s) 437
DpnI GATC 6 cut(s) 356, 379, 418, 720, 888, 921
DpnII GATC 6 cut(s) 354, 377, 416, 718, 886, 919
Eam1104I CTCTTC 5 cut(s) 75, 349, 536, 812, 992
EarI CTCTTC 5 cut(s) 75, 349, 536, 812, 992
Ecl136II GAGCTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 729
Eco147I AGGCCT 1 cut(s) 1083
Eco24I GRGCYC 1 cut(s) 115
Eco47I GGWCC 3 cut(s) 172, 321, 1061
Eco53kI GAGCTC 1 cut(s) 113
Eco57I CTGAAG 4 cut(s) 63, 776, 1016, 1032
Eco72I CACGTG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 113
EcoO109I RGGNCCY 2 cut(s) 321, 1061
EcoRI GAATTC 1 cut(s) 160
EcoRII CCWGG 4 cut(s) 14, 526, 649, 1122
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 115
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 3 cut(s) 172, 581, 625
FaqI GGGAC 2 cut(s) 256, 307
FatI CATG 3 cut(s) 168, 577, 621
FauI CCCGC 3 cut(s) 435, 939, 1057
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 7 cut(s) 135, 241, 244, 367, 422, 829, 1023
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 7 cut(s) 135, 241, 244, 367, 422, 829, 1023
FspBI CTAG 1 cut(s) 986
GlaI GCGC 2 cut(s) 437, 547
GluI GCNGC 7 cut(s) 135, 241, 244, 367, 422, 829, 1023
GsuI CTGGAG 1 cut(s) 633
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 1083
HapII CCGG 6 cut(s) 152, 262, 433, 439, 445, 632
HgaI GACGC 2 cut(s) 358, 614
HhaI GCGC 2 cut(s) 438, 548
Hin1I GRCGYC 2 cut(s) 369, 436
Hin1II CATG 3 cut(s) 172, 581, 625
Hin6I GCGC 2 cut(s) 436, 546
HinP1I GCGC 2 cut(s) 436, 546
HincII GTYRAC 2 cut(s) 373, 514
HindII GTYRAC 2 cut(s) 373, 514
HinfI GANTC 5 cut(s) 148, 349, 550, 751, 1055
HpaII CCGG 6 cut(s) 152, 262, 433, 439, 445, 632
HphI GGTGA 3 cut(s) 86, 248, 772
Hpy166II GTNNAC 2 cut(s) 373, 514
Hpy188I TCNGA 6 cut(s) 55, 147, 280, 354, 723, 756
Hpy188III TCNNGA 5 cut(s) 412, 785, 859, 986, 1067
Hpy8I GTNNAC 2 cut(s) 373, 514
Hpy99I CGWCG 8 cut(s) 284, 334, 337, 374, 377, 413, 487, 490
HpyAV CCTTC 6 cut(s) 16, 68, 751, 1073, 1096, 1104
HpyCH4III ACNGT 4 cut(s) 400, 566, 673, 903
HpyCH4IV ACGT 3 cut(s) 140, 329, 482
HpyCH4V TGCA 5 cut(s) 33, 605, 848, 965, 1025
HpyF10VI GCNNNNNNNGC 5 cut(s) 231, 240, 427, 943, 1022
HpyF3I CTNAG 4 cut(s) 54, 351, 786, 1017
HpySE526I ACGT 3 cut(s) 140, 329, 482
Hsp92I GRCGYC 2 cut(s) 369, 436
Hsp92II CATG 3 cut(s) 172, 581, 625
HspAI GCGC 2 cut(s) 436, 546
KasI GGCGCC 1 cut(s) 435
KflI GGGWCCC 1 cut(s) 321
KroI GCCGGC 1 cut(s) 438
KroNI GCCGGC 1 cut(s) 440
Kzo9I GATC 6 cut(s) 354, 377, 416, 718, 886, 919
LguI GCTCTTC 2 cut(s) 536, 812
LmnI GCTCC 5 cut(s) 429, 447, 652, 787, 1105
Lsp1109I GCAGC 4 cut(s) 252, 255, 408, 1009
LweI GCATC 1 cut(s) 952
MaeI CTAG 1 cut(s) 986
MaeII ACGT 3 cut(s) 140, 329, 482
MaeIII GTNAC 1 cut(s) 617
MalI GATC 6 cut(s) 356, 379, 418, 720, 888, 921
MbiI CCGCTC 1 cut(s) 366
MboI GATC 6 cut(s) 354, 377, 416, 718, 886, 919
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 2 cut(s) 115, 972
MluCI AATT 9 cut(s) 49, 86, 160, 663, 744, 837, 863, 882, 939
Mly113I GGCGCC 1 cut(s) 436
MlyI GAGTC 2 cut(s) 343, 760
MmeI TCCRAC 2 cut(s) 303, 1054
MnlI CCTC 8 cut(s) 352, 517, 639, 822, 993, 1012, 1056, 1074
MroNI GCCGGC 1 cut(s) 438
MseI TTAA 3 cut(s) 735, 836, 1153
MspI CCGG 6 cut(s) 152, 262, 433, 439, 445, 632
MspR9I CCNGG 6 cut(s) 16, 433, 528, 633, 651, 1124
MunI CAATTG 1 cut(s) 882
MvaI CCWGG 4 cut(s) 16, 528, 651, 1124
MvnI CGCG 2 cut(s) 413, 548
MwoI GCNNNNNNNGC 5 cut(s) 231, 240, 427, 943, 1022
NaeI GCCGGC 1 cut(s) 440
NarI GGCGCC 1 cut(s) 436
NciI CCSGG 2 cut(s) 433, 633
NdeII GATC 6 cut(s) 354, 377, 416, 718, 886, 919
NgoMIV GCCGGC 1 cut(s) 438
NlaIII CATG 3 cut(s) 172, 581, 625
NlaIV GGNNCC 4 cut(s) 322, 323, 437, 443
NmeAIII GCCGAG 1 cut(s) 1108
NruI TCGCGA 1 cut(s) 413
NspI RCATGY 1 cut(s) 625
PceI AGGCCT 1 cut(s) 1083
PciSI GCTCTTC 2 cut(s) 536, 812
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PdiI GCCGGC 1 cut(s) 440
PfeI GAWTC 3 cut(s) 148, 550, 1055
PfoI TCCNGGA 1 cut(s) 649
PkrI GCNGC 7 cut(s) 136, 242, 245, 368, 423, 830, 1024
PleI GAGTC 2 cut(s) 343, 759
PluTI GGCGCC 1 cut(s) 439
PmaCI CACGTG 1 cut(s) 141
PmlI CACGTG 1 cut(s) 141
PpsI GAGTC 2 cut(s) 343, 759
Ppu21I YACGTR 1 cut(s) 141
PpuMI RGGWCCY 2 cut(s) 321, 1061
Psp124BI GAGCTC 1 cut(s) 115
Psp5II RGGWCCY 2 cut(s) 321, 1061
Psp6I CCWGG 4 cut(s) 14, 526, 649, 1122
PspCI CACGTG 1 cut(s) 141
PspGI CCWGG 4 cut(s) 14, 526, 649, 1122
PspN4I GGNNCC 4 cut(s) 322, 323, 437, 443
PspPI GGNCC 3 cut(s) 172, 321, 1061
PspPPI RGGWCCY 2 cut(s) 321, 1061
PsuI RGATCY 1 cut(s) 354
RruI TCGCGA 1 cut(s) 413
RsaI GTAC 1 cut(s) 481
RsaNI GTAC 1 cut(s) 480
SacI GAGCTC 1 cut(s) 115
SalI GTCGAC 1 cut(s) 371
SapI GCTCTTC 2 cut(s) 536, 812
SaqAI TTAA 3 cut(s) 735, 836, 1153
SatI GCNGC 7 cut(s) 135, 241, 244, 367, 422, 829, 1023
Sau3AI GATC 6 cut(s) 354, 377, 416, 718, 886, 919
Sau96I GGNCC 3 cut(s) 172, 321, 1061
SchI GAGTC 2 cut(s) 343, 760
ScrFI CCNGG 6 cut(s) 16, 433, 528, 633, 651, 1124
SduI GDGCHC 2 cut(s) 115, 972
SfaNI GCATC 1 cut(s) 952
SfcI CTRYAG 2 cut(s) 199, 359
SfoI GGCGCC 1 cut(s) 437
SgrDI CGTCGACG 1 cut(s) 371
SinI GGWCC 3 cut(s) 172, 321, 1061
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 9 cut(s) 49, 86, 160, 663, 744, 837, 863, 882, 939
SseBI AGGCCT 1 cut(s) 1083
SsiI CCGC 8 cut(s) 135, 181, 223, 366, 428, 828, 946, 1050
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 1 cut(s) 986
SstI GAGCTC 1 cut(s) 115
StuI AGGCCT 1 cut(s) 1083
StyD4I CCNGG 6 cut(s) 14, 431, 526, 631, 649, 1122
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 4 cut(s) 400, 566, 673, 903
TaiI ACGT 3 cut(s) 143, 332, 485
TaqI TCGA 7 cut(s) 115, 347, 372, 380, 386, 450, 1029
TasI AATT 9 cut(s) 49, 86, 160, 663, 744, 837, 863, 882, 939
TauI GCSGC 3 cut(s) 137, 369, 831
TfiI GAWTC 3 cut(s) 148, 550, 1055
Tru1I TTAA 3 cut(s) 735, 836, 1153
Tru9I TTAA 3 cut(s) 735, 836, 1153
TseI GCWGC 4 cut(s) 240, 243, 421, 1022
TspDTI ATGAA 2 cut(s) 537, 1110
TspGWI ACGGA 5 cut(s) 294, 390, 397, 499, 833
VpaK11BI GGWCC 3 cut(s) 172, 321, 1061
XapI RAATTY 5 cut(s) 49, 86, 160, 863, 939
XbaI TCTAGA 1 cut(s) 985
XceI RCATGY 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.