MD09G1096700.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003521
Physical Location & Seq
Reverse (-)
7022070 .. 7025087
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1096700.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGCTGTGGAGGCTGAGAAATCTGGGCCTGACGGGAAGCTTTGGAGTCTGTTCAAAATACCGTTTTGGCAGTCCGGAAACGCCTCTTCGTCTTCCTCTTCGTCATCGTCCTCGTCCTTGTCTTCTATGCATCATAATGTTCACCACCAGCTGGGTCACGAGTCTGCGGCTGAGCGTACGGCTCTTCATTCTGCCAGTACGGTGTCGTATGTGACGAAGTCTTTGCTTCCTGCTCGGAGAAGGCTGCGTCTTGATCCTCCAAATAAGCTATTTTTTCCATATGAACCTGGTAAACAGGTCAGGAGTGCAATTGGAATAAAGAACACAAGCAAAACTATTGTAGCTTTCAAGTTCCAAACAACTGCACCAAAGAGTTGTTACATGCGTCCTCCTGGGGGTATACTTGCTCCTGGTGAAGGTCTTATTGCAACTGTTTTCAAGTTTGTGGAACCTCCTGAGAACGATGAGAAACCAGCTGTAGACCAGAAGAGCAGGGTTAAGTTCAAGATCATGAGCTTAAAAGTGAAAGGAGATATGGACTATGTGCCAGAACTGTTTGATGAGCAGAGGGACCAAGTCGTGGTTGAGCACGTTTTGCGTGTTGTTTTTCTTGACCCTGAGCGCCCTACCCCTGCAATGGAAAAACTTGAGCGGCAATTGGCCGAGGCTGAGGCTGCCCTTGAATCACGAAAAAAGCCTCCAGAAGAAGCAGGTCCCCGGATTGTAGGGGAAGGACTTGTCATAGATGAATGGAAAGAGCGCAGGGAAAGATACCTAGCGCAGCAGCAGGTTGAAGGGGTTGATTCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

271

Amino Acids

30.09

Weight (kDa)

8.51

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 82 - 191 3.2e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 701, 778
AccB7I CCANNNNNTGG 2 cut(s) 151, 580
AccBSI CCGCTC 1 cut(s) 652
AccI GTMKAC 2 cut(s) 400, 480
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 2 cut(s) 167, 652
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 1 cut(s) 660
AfaI GTAC 2 cut(s) 178, 199
AfiI CCNNNNNNNGG 5 cut(s) 151, 395, 416, 580, 637
AgsI TTSAA 6 cut(s) 55, 349, 439, 505, 683, 794
AjnI CCWGG 3 cut(s) 286, 391, 409
AluBI AGCT 6 cut(s) 40, 151, 268, 344, 476, 516
AluI AGCT 6 cut(s) 40, 151, 268, 344, 476, 516
Alw21I GWGCWC 1 cut(s) 591
AlwI GGATC 1 cut(s) 248
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 2 cut(s) 26, 660
ApeKI GCWGC 4 cut(s) 244, 674, 781, 784
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
AspLEI GCGC 3 cut(s) 624, 762, 781
AspS9I GGNCC 3 cut(s) 26, 571, 713
AsuC2I CCSGG 1 cut(s) 718
AsuHPI GGTGA 2 cut(s) 134, 425
AvaII GGWCC 2 cut(s) 571, 713
BauI CACGAG 1 cut(s) 158
BbsI GAAGAC 2 cut(s) 84, 114
Bbv12I GWGCWC 1 cut(s) 591
BbvCI CCTCAGC 1 cut(s) 669
BbvI GCAGC 4 cut(s) 231, 661, 793, 796
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 3 cut(s) 288, 393, 411
BcnI CCSGG 1 cut(s) 718
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 625
BfuAI ACCTGC 2 cut(s) 701, 778
BisI GCNGC 6 cut(s) 168, 245, 653, 675, 782, 785
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 6 cut(s) 169, 246, 654, 676, 783, 786
Bme1390I CCNGG 4 cut(s) 288, 393, 411, 718
Bme18I GGWCC 2 cut(s) 571, 713
BmgT120I GGNCC 3 cut(s) 26, 571, 713
BmiI GGNNCC 3 cut(s) 450, 572, 715
BmrFI CCNGG 4 cut(s) 288, 393, 411, 718
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 84, 114
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 2 cut(s) 618, 669
Bpu1102I GCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 718
BsaJI CCNNGG 3 cut(s) 392, 663, 716
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 395, 416, 580, 637
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 3 cut(s) 288, 393, 411
BseDI CCNNGG 3 cut(s) 392, 663, 716
BseLI CCNNNNNNNGG 5 cut(s) 151, 395, 416, 580, 637
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 5 cut(s) 6, 162, 447, 609, 660
BseNI ACTGG 1 cut(s) 195
BseXI GCAGC 4 cut(s) 231, 661, 793, 796
BseYI CCCAGC 1 cut(s) 151
BsgI GTGCAG 1 cut(s) 348
BshFI GGCC 2 cut(s) 28, 662
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 2 cut(s) 75, 718
BsiWI CGTACG 1 cut(s) 176
BslFI GGGAC 2 cut(s) 584, 699
BslI CCNNNNNNNGG 5 cut(s) 151, 395, 416, 580, 637
BsmFI GGGAC 2 cut(s) 584, 699
BsnI GGCC 2 cut(s) 28, 662
Bsp1286I GDGCHC 1 cut(s) 591
Bsp13I TCCGGA 1 cut(s) 74
Bsp143I GATC 2 cut(s) 253, 507
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 2 cut(s) 167, 652
BspANI GGCC 2 cut(s) 28, 662
BspCNI CTCAG 5 cut(s) 7, 163, 448, 610, 661
BspEI TCCGGA 1 cut(s) 74
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 3 cut(s) 450, 572, 715
BspMI ACCTGC 2 cut(s) 701, 778
BspPI GGATC 1 cut(s) 248
BspQI GCTCTTC 2 cut(s) 189, 482
BsrBI CCGCTC 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 642
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 3 cut(s) 392, 663, 716
BssMI GATC 2 cut(s) 253, 507
BssNAI GTATAC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 158
Bst1107I GTATAC 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 158
Bst2UI CCWGG 3 cut(s) 288, 393, 411
Bst4CI ACNGT 4 cut(s) 63, 202, 433, 555
Bst6I CTCTTC 4 cut(s) 91, 103, 189, 482
BstAPI GCANNNNNTGC 1 cut(s) 595
BstDEI CTNAG 5 cut(s) 15, 171, 456, 618, 669
BstENI CCTNNNNNAGG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 625
BstHHI GCGC 3 cut(s) 624, 762, 781
BstKTI GATC 2 cut(s) 256, 510
BstMBI GATC 2 cut(s) 253, 507
BstMWI GCNNNNNNNGC 3 cut(s) 11, 595, 674
BstNI CCWGG 3 cut(s) 288, 393, 411
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 4 cut(s) 286, 391, 409, 716
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 4 cut(s) 231, 661, 793, 796
BstV2I GAAGAC 2 cut(s) 84, 114
BstZ17I GTATAC 1 cut(s) 401
BsuRI GGCC 2 cut(s) 28, 662
BtsIMutI CAGTG 1 cut(s) 813
BveI ACCTGC 2 cut(s) 701, 778
CciI TCATGA 1 cut(s) 510
CfoI GCGC 3 cut(s) 624, 762, 781
Cfr13I GGNCC 3 cut(s) 26, 571, 713
CseI GACGC 2 cut(s) 236, 374
CsiI ACCWGGT 1 cut(s) 286
Csp6I GTAC 2 cut(s) 177, 198
CviAII CATG 2 cut(s) 382, 511
CviQI GTAC 2 cut(s) 177, 198
DdeI CTNAG 5 cut(s) 15, 171, 456, 618, 669
DpnI GATC 2 cut(s) 255, 509
DpnII GATC 2 cut(s) 253, 507
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 4 cut(s) 91, 103, 189, 482
EarI CTCTTC 4 cut(s) 91, 103, 189, 482
Eco47I GGWCC 2 cut(s) 571, 713
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 3 cut(s) 286, 391, 409
EcoT22I ATGCAT 1 cut(s) 132
FaeI CATG 2 cut(s) 385, 514
FaqI GGGAC 2 cut(s) 584, 699
FatI CATG 2 cut(s) 381, 510
FauNDI CATATG 1 cut(s) 280
FblI GTMKAC 2 cut(s) 400, 480
Fnu4HI GCNGC 6 cut(s) 168, 245, 653, 675, 782, 785
Fsp4HI GCNGC 6 cut(s) 168, 245, 653, 675, 782, 785
FspBI CTAG 1 cut(s) 776
GlaI GCGC 3 cut(s) 623, 761, 780
GluI GCNGC 6 cut(s) 168, 245, 653, 675, 782, 785
GsaI CCCAGC 1 cut(s) 155
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 625
HaeIII GGCC 2 cut(s) 28, 662
HapII CCGG 2 cut(s) 75, 718
HgaI GACGC 2 cut(s) 236, 374
HhaI GCGC 3 cut(s) 624, 762, 781
Hin1II CATG 2 cut(s) 385, 514
Hin6I GCGC 3 cut(s) 622, 760, 779
HinP1I GCGC 3 cut(s) 622, 760, 779
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 4 cut(s) 46, 161, 683, 803
HpaII CCGG 2 cut(s) 75, 718
HphI GGTGA 2 cut(s) 134, 425
Hpy166II GTNNAC 4 cut(s) 142, 293, 401, 481
Hpy188I TCNGA 1 cut(s) 237
Hpy8I GTNNAC 4 cut(s) 142, 293, 401, 481
HpyAV CCTTC 4 cut(s) 234, 410, 725, 788
HpyCH4III ACNGT 4 cut(s) 63, 202, 433, 555
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 5 cut(s) 130, 308, 365, 428, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 595, 674
HpyF3I CTNAG 5 cut(s) 15, 171, 456, 618, 669
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 2 cut(s) 385, 514
HspAI GCGC 3 cut(s) 622, 760, 779
Kpn2I TCCGGA 1 cut(s) 74
Kzo9I GATC 2 cut(s) 253, 507
LguI GCTCTTC 2 cut(s) 189, 482
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 4 cut(s) 231, 661, 793, 796
LweI GCATC 1 cut(s) 139
MabI ACCWGGT 1 cut(s) 286
MaeI CTAG 1 cut(s) 776
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 3 cut(s) 155, 211, 377
MalI GATC 2 cut(s) 255, 509
MbiI CCGCTC 1 cut(s) 652
MboI GATC 2 cut(s) 253, 507
MboII GAAGA 7 cut(s) 78, 84, 90, 114, 176, 499, 716
MfeI CAATTG 2 cut(s) 309, 656
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 2 cut(s) 309, 656
MlyI GAGTC 2 cut(s) 55, 170
Mph1103I ATGCAT 1 cut(s) 132
MroI TCCGGA 1 cut(s) 74
MseI TTAA 2 cut(s) 498, 518
MslI CAYNNNNRTG 1 cut(s) 135
MspA1I CMGCKG 2 cut(s) 151, 476
MspI CCGG 2 cut(s) 75, 718
MspR9I CCNGG 4 cut(s) 288, 393, 411, 718
MunI CAATTG 2 cut(s) 309, 656
MvaI CCWGG 3 cut(s) 288, 393, 411
MwoI GCNNNNNNNGC 3 cut(s) 11, 595, 674
NciI CCSGG 1 cut(s) 718
NdeI CATATG 1 cut(s) 280
NdeII GATC 2 cut(s) 253, 507
NlaIII CATG 2 cut(s) 385, 514
NlaIV GGNNCC 3 cut(s) 450, 572, 715
NmeAIII GCCGAG 1 cut(s) 688
NmuCI GTSAC 2 cut(s) 155, 211
NsiI ATGCAT 1 cut(s) 132
NspI RCATGY 1 cut(s) 385
PagI TCATGA 1 cut(s) 510
PciSI GCTCTTC 2 cut(s) 189, 482
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PfeI GAWTC 2 cut(s) 683, 803
Pfl23II CGTACG 1 cut(s) 176
PflFI GACNNNGTC 2 cut(s) 217, 575
PflMI CCANNNNNTGG 2 cut(s) 151, 580
PkrI GCNGC 6 cut(s) 169, 246, 654, 676, 783, 786
PleI GAGTC 2 cut(s) 54, 169
PpsI GAGTC 2 cut(s) 54, 169
PpuMI RGGWCCY 1 cut(s) 713
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 3 cut(s) 286, 391, 409
PspFI CCCAGC 1 cut(s) 151
PspGI CCWGG 3 cut(s) 286, 391, 409
PspLI CGTACG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 450, 572, 715
PspPI GGNCC 3 cut(s) 26, 571, 713
PspPPI RGGWCCY 1 cut(s) 713
PsyI GACNNNGTC 2 cut(s) 217, 575
PvuII CAGCTG 2 cut(s) 151, 476
RsaI GTAC 2 cut(s) 178, 199
RsaNI GTAC 2 cut(s) 177, 198
RseI CAYNNNNRTG 1 cut(s) 135
SapI GCTCTTC 2 cut(s) 189, 482
SaqAI TTAA 2 cut(s) 498, 518
SatI GCNGC 6 cut(s) 168, 245, 653, 675, 782, 785
Sau3AI GATC 2 cut(s) 253, 507
Sau96I GGNCC 3 cut(s) 26, 571, 713
SchI GAGTC 2 cut(s) 55, 170
ScrFI CCNGG 4 cut(s) 288, 393, 411, 718
SduI GDGCHC 1 cut(s) 591
SexAI ACCWGGT 1 cut(s) 286
SfaNI GCATC 1 cut(s) 139
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 2 cut(s) 571, 713
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
Sse9I AATT 2 cut(s) 309, 656
SsiI CCGC 2 cut(s) 167, 652
SspMI CTAG 1 cut(s) 776
StyD4I CCNGG 4 cut(s) 286, 391, 409, 716
TaaI ACNGT 4 cut(s) 63, 202, 433, 555
TaiI ACGT 1 cut(s) 594
TasI AATT 2 cut(s) 309, 656
TauI GCSGC 2 cut(s) 170, 655
TfiI GAWTC 2 cut(s) 683, 803
Tru1I TTAA 2 cut(s) 498, 518
Tru9I TTAA 2 cut(s) 498, 518
TscAI CASTG 1 cut(s) 813
TseFI GTSAC 2 cut(s) 155, 211
TseI GCWGC 4 cut(s) 244, 674, 781, 784
Tsp45I GTSAC 2 cut(s) 155, 211
TspDTI ATGAA 3 cut(s) 176, 297, 762
TspRI CASTG 1 cut(s) 813
Tth111I GACNNNGTC 2 cut(s) 217, 575
Van91I CCANNNNNTGG 2 cut(s) 151, 580
VpaK11BI GGWCC 2 cut(s) 571, 713
XagI CCTNNNNNAGG 1 cut(s) 414
XceI RCATGY 1 cut(s) 385
XmiI GTMKAC 2 cut(s) 400, 480
XspI CTAG 1 cut(s) 776
Zsp2I ATGCAT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.