Rh5CG278700

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003521
Physical Location & Seq
Reverse (-)
30339979 .. 30341741
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG278700.1

Sequence Viewer

Length: 828 bp
ATGGACCACCACCGCCACCACCACCACCACGACTACAGCCACTCCCACCACCACCACAGCCAGAGCAGCAGCCACCACCATCACCACCGGCACAAGTCCCACTCGGACGGCAACCTCTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGCGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTGCTCCCGCCTCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTCTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCGATTCGGTTGAAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCTGACAGCAATGAGAAAAATGTGGATCAGAAGACCAAGGCTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGATCAAGTAGCAGTTGAGCGAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTCTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTCGAAGTGCGTAAGAAACCTCCTGCAGATTCAGGTCCTCGTGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTTGAAGCAGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

275

Amino Acids

31.11

Weight (kDa)

9.57

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 90 - 200 9e-25 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 198
AccII CGCG 2 cut(s) 239, 332
AciI CCGC 4 cut(s) 13, 192, 254, 619
AclWI GGATC 4 cut(s) 175, 211, 510, 600
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 3 cut(s) 560, 689, 705
AcyI GRCGYC 2 cut(s) 195, 262
AfiI CCNNNNNNNGG 1 cut(s) 419
AgsI TTSAA 5 cut(s) 343, 373, 380, 526, 815
AjnI CCWGG 3 cut(s) 310, 433, 795
AjuI GAANNNNNNNTTGG 4 cut(s) 506, 538, 662, 694
AluBI AGCT 5 cut(s) 368, 399, 551, 574, 689
AluI AGCT 5 cut(s) 368, 399, 551, 574, 689
Alw21I GWGCWC 1 cut(s) 645
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 4 cut(s) 175, 211, 510, 600
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 4 cut(s) 66, 69, 247, 695
ApoI RAATTY 1 cut(s) 612
AspLEI GCGC 2 cut(s) 264, 332
AspS9I GGNCC 3 cut(s) 4, 147, 734
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 2 cut(s) 74, 556
AvaII GGWCC 3 cut(s) 4, 147, 734
BanI GGYRCC 1 cut(s) 261
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 2 cut(s) 156, 515
Bbv12I GWGCWC 1 cut(s) 645
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 4 cut(s) 78, 81, 234, 682
BccI CCATC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BciT130I CCWGG 3 cut(s) 312, 435, 797
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 659
BfmI CTRYAG 3 cut(s) 34, 185, 723
BfoI RGCGCY 1 cut(s) 265
BisI GCNGC 5 cut(s) 67, 70, 193, 248, 696
BlsI GCNGC 5 cut(s) 68, 71, 194, 249, 697
Bme1390I CCNGG 4 cut(s) 312, 417, 435, 797
Bme18I GGWCC 3 cut(s) 4, 147, 734
BmgT120I GGNCC 3 cut(s) 4, 147, 734
BmiI GGNNCC 4 cut(s) 148, 149, 263, 269
BmrFI CCNGG 4 cut(s) 312, 417, 435, 797
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 2 cut(s) 156, 515
BpmI CTGGAG 1 cut(s) 417
Bpu10I CCTNAGC 2 cut(s) 570, 690
BpuMI CCSGG 1 cut(s) 417
BsaHI GRCGYC 2 cut(s) 195, 262
BsaJI CCNNGG 3 cut(s) 257, 311, 513
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse118I RCCGGY 2 cut(s) 87, 264
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 3 cut(s) 312, 435, 797
BseDI CCNNGG 3 cut(s) 257, 311, 513
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 3 cut(s) 191, 561, 681
BseXI GCAGC 4 cut(s) 78, 81, 234, 682
BsgI GTGCAG 2 cut(s) 372, 681
Bsh1236I CGCG 2 cut(s) 239, 332
BshFI GGCC 1 cut(s) 756
BshNI GGYRCC 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 645
BsiSI CCGG 4 cut(s) 88, 265, 271, 416
BslFI GGGAC 2 cut(s) 82, 133
BslI CCNNNNNNNGG 1 cut(s) 419
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 82, 133
BsnI GGCC 1 cut(s) 756
Bsp1286I GDGCHC 1 cut(s) 645
Bsp143I GATC 5 cut(s) 180, 203, 242, 502, 592
Bsp68I TCGCGA 1 cut(s) 239
BspACI CCGC 4 cut(s) 13, 192, 254, 619
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 3 cut(s) 190, 562, 682
BspFNI CGCG 2 cut(s) 239, 332
BspLI GGNNCC 4 cut(s) 148, 149, 263, 269
BspMAI CTGCAG 1 cut(s) 727
BspPI GGATC 4 cut(s) 175, 211, 510, 600
BspQI GCTCTTC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 2 cut(s) 87, 264
BssAI RCCGGY 2 cut(s) 87, 264
BssECI CCNNGG 3 cut(s) 257, 311, 513
BssMI GATC 5 cut(s) 180, 203, 242, 502, 592
BssNI GRCGYC 2 cut(s) 195, 262
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 3 cut(s) 312, 435, 797
Bst4CI ACNGT 3 cut(s) 226, 350, 457
Bst6I CTCTTC 4 cut(s) 122, 175, 320, 665
BstACI GRCGYC 2 cut(s) 195, 262
BstC8I GCNNGC 2 cut(s) 266, 286
BstDEI CTNAG 4 cut(s) 177, 519, 570, 690
BstFNI CGCG 2 cut(s) 239, 332
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 2 cut(s) 264, 332
BstKTI GATC 5 cut(s) 183, 206, 245, 505, 595
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 5 cut(s) 180, 203, 242, 502, 592
BstMWI GCNNNNNNNGC 4 cut(s) 66, 253, 616, 695
BstNI CCWGG 3 cut(s) 312, 435, 797
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 4 cut(s) 310, 415, 433, 795
BstSFI CTRYAG 3 cut(s) 34, 185, 723
BstUI CGCG 2 cut(s) 239, 332
BstV1I GCAGC 4 cut(s) 78, 81, 234, 682
BstV2I GAAGAC 2 cut(s) 156, 515
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 756
BtuMI TCGCGA 1 cut(s) 239
Cac8I GCNNGC 2 cut(s) 266, 286
CfoI GCGC 2 cut(s) 264, 332
Cfr10I RCCGGY 2 cut(s) 87, 264
Cfr13I GGNCC 3 cut(s) 4, 147, 734
CseI GACGC 2 cut(s) 184, 398
CviAII CATG 2 cut(s) 362, 406
DdeI CTNAG 4 cut(s) 177, 519, 570, 690
DinI GGCGCC 1 cut(s) 263
DpnI GATC 5 cut(s) 182, 205, 244, 504, 594
DpnII GATC 5 cut(s) 180, 203, 242, 502, 592
Eam1104I CTCTTC 4 cut(s) 122, 175, 320, 665
EarI CTCTTC 4 cut(s) 122, 175, 320, 665
Eco130I CCWWGG 1 cut(s) 513
Eco147I AGGCCT 1 cut(s) 756
Eco47I GGWCC 3 cut(s) 4, 147, 734
Eco57I CTGAAG 3 cut(s) 560, 689, 705
EcoO109I RGGNCCY 2 cut(s) 147, 734
EcoRII CCWGG 3 cut(s) 310, 433, 795
EcoT14I CCWWGG 1 cut(s) 513
EgeI GGCGCC 1 cut(s) 263
EheI GGCGCC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 365, 409
FaiI YATR 6 cut(s) 306, 363, 407, 446, 533, 564
FaqI GGGAC 2 cut(s) 82, 133
FatI CATG 2 cut(s) 361, 405
FauI CCCGC 2 cut(s) 261, 612
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 5 cut(s) 67, 70, 193, 248, 696
Fsp4HI GCNGC 5 cut(s) 67, 70, 193, 248, 696
FspBI CTAG 1 cut(s) 659
GlaI GCGC 2 cut(s) 263, 331
GluI GCNGC 5 cut(s) 67, 70, 193, 248, 696
GsuI CTGGAG 1 cut(s) 417
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 1 cut(s) 756
HapII CCGG 4 cut(s) 88, 265, 271, 416
HgaI GACGC 2 cut(s) 184, 398
HhaI GCGC 2 cut(s) 264, 332
Hin1I GRCGYC 2 cut(s) 195, 262
Hin1II CATG 2 cut(s) 365, 409
Hin6I GCGC 2 cut(s) 262, 330
HinP1I GCGC 2 cut(s) 262, 330
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 4 cut(s) 175, 334, 535, 728
HpaII CCGG 4 cut(s) 88, 265, 271, 416
HphI GGTGA 2 cut(s) 74, 556
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 4 cut(s) 106, 180, 507, 540
Hpy188III TCNNGA 4 cut(s) 238, 569, 659, 740
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 5 cut(s) 160, 163, 200, 203, 239
HpyAV CCTTC 4 cut(s) 535, 746, 769, 777
HpyCH4III ACNGT 3 cut(s) 226, 350, 457
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 4 cut(s) 389, 638, 698, 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 253, 616, 695
HpyF3I CTNAG 4 cut(s) 177, 519, 570, 690
HpySE526I ACGT 1 cut(s) 155
Hsp92I GRCGYC 2 cut(s) 195, 262
Hsp92II CATG 2 cut(s) 365, 409
HspAI GCGC 2 cut(s) 262, 330
KasI GGCGCC 1 cut(s) 261
KflI GGGWCCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 264
KroNI GCCGGC 1 cut(s) 266
Kzo9I GATC 5 cut(s) 180, 203, 242, 502, 592
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 5 cut(s) 255, 273, 436, 571, 778
Lsp1109I GCAGC 4 cut(s) 78, 81, 234, 682
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 659
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 401
MalI GATC 5 cut(s) 182, 205, 244, 504, 594
MbiI CCGCTC 1 cut(s) 192
MboI GATC 5 cut(s) 180, 203, 242, 502, 592
MboII GAAGA 7 cut(s) 109, 156, 162, 337, 355, 520, 682
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 645
MluCI AATT 3 cut(s) 447, 528, 612
Mly113I GGCGCC 1 cut(s) 262
MlyI GAGTC 2 cut(s) 169, 544
MnlI CCTC 8 cut(s) 125, 178, 267, 423, 666, 685, 729, 747
MroNI GCCGGC 1 cut(s) 264
MseI TTAA 1 cut(s) 826
MspI CCGG 4 cut(s) 88, 265, 271, 416
MspR9I CCNGG 4 cut(s) 312, 417, 435, 797
MvaI CCWGG 3 cut(s) 312, 435, 797
MvnI CGCG 2 cut(s) 239, 332
MwoI GCNNNNNNNGC 4 cut(s) 66, 253, 616, 695
NaeI GCCGGC 1 cut(s) 266
NarI GGCGCC 1 cut(s) 262
NciI CCSGG 1 cut(s) 417
NdeII GATC 5 cut(s) 180, 203, 242, 502, 592
NgoMIV GCCGGC 1 cut(s) 264
NlaIII CATG 2 cut(s) 365, 409
NlaIV GGNNCC 4 cut(s) 148, 149, 263, 269
NmeAIII GCCGAG 2 cut(s) 238, 781
NruI TCGCGA 1 cut(s) 239
NspI RCATGY 1 cut(s) 409
PceI AGGCCT 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PdiI GCCGGC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 334, 728
PfoI TCCNGGA 1 cut(s) 433
PkrI GCNGC 5 cut(s) 68, 71, 194, 249, 697
PleI GAGTC 2 cut(s) 169, 543
PluTI GGCGCC 1 cut(s) 265
PpsI GAGTC 2 cut(s) 169, 543
PpuMI RGGWCCY 2 cut(s) 147, 734
Psp5II RGGWCCY 2 cut(s) 147, 734
Psp6I CCWGG 3 cut(s) 310, 433, 795
PspGI CCWGG 3 cut(s) 310, 433, 795
PspN4I GGNNCC 4 cut(s) 148, 149, 263, 269
PspPI GGNCC 3 cut(s) 4, 147, 734
PspPPI RGGWCCY 2 cut(s) 147, 734
PstI CTGCAG 1 cut(s) 727
PsuI RGATCY 1 cut(s) 180
RruI TCGCGA 1 cut(s) 239
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 826
SatI GCNGC 5 cut(s) 67, 70, 193, 248, 696
Sau3AI GATC 5 cut(s) 180, 203, 242, 502, 592
Sau96I GGNCC 3 cut(s) 4, 147, 734
SchI GAGTC 2 cut(s) 169, 544
ScrFI CCNGG 4 cut(s) 312, 417, 435, 797
SduI GDGCHC 1 cut(s) 645
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 3 cut(s) 34, 185, 723
SfoI GGCGCC 1 cut(s) 263
SgrDI CGTCGACG 1 cut(s) 197
SinI GGWCC 3 cut(s) 4, 147, 734
Sse9I AATT 3 cut(s) 447, 528, 612
SseBI AGGCCT 1 cut(s) 756
SsiI CCGC 4 cut(s) 13, 192, 254, 619
SspDI GGCGCC 1 cut(s) 261
SspMI CTAG 1 cut(s) 659
StuI AGGCCT 1 cut(s) 756
StyD4I CCNGG 4 cut(s) 310, 415, 433, 795
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 3 cut(s) 226, 350, 457
TaiI ACGT 1 cut(s) 158
TaqI TCGA 6 cut(s) 173, 198, 206, 212, 276, 702
TasI AATT 3 cut(s) 447, 528, 612
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 2 cut(s) 334, 728
Tru1I TTAA 1 cut(s) 826
Tru9I TTAA 1 cut(s) 826
TseI GCWGC 4 cut(s) 66, 69, 247, 695
TspDTI ATGAA 2 cut(s) 321, 783
TspGWI ACGGA 4 cut(s) 120, 216, 223, 572
VpaK11BI GGWCC 3 cut(s) 4, 147, 734
XapI RAATTY 1 cut(s) 612
XbaI TCTAGA 1 cut(s) 658
XceI RCATGY 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.