FvH4_1g12190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003134
Physical Location & Seq
Forward (+)
6664074 .. 6666608
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12190.t1

Sequence Viewer

Length: 594 bp
ATGGCCACCGATCCAAGCAAAGACGACGACAACAACAACAACAACCAGAATCCGGTAAAACTTTCTAAGAAGGAGAAGGGTAAATCATGCAAAGGTACCCTTTACTATTCCTCGGCCCTCCAATCCAAAGCCAAGAACCCTCGCTGCATCGGCATTCCCCGCTCTCTTCCCCAAGTGCCTAACTCAATAAAAGATGAGCAGCAGCAGTTTCATGATTTTTACTATGCCTGTGCCGGTTACTCGGTCTACTTGGATCAAAAATCTGGAAAGGAATCTTCGGGTGATAAGCAAACGCCAGCAAAATTACCTGTTTGTGTCGGCTTGGAGCTTCTAGTTGGCAGAAAAGCTCCTGCTAGTGCACCTGCTGTCACTCCTACCGCGGCTCATGCTCTCAACAAAGAGGATGCTCGTGAAGTCTGTCAACCTCAGAGACCTAAACCCATTCAACCTGCAGGGAATGATTTCTTGACTAGGTTCTCAAGAAATGCAGATCTAGTCGCATTAGGGGTTTGGAAGAACATGGGTAAAGTTGGGAACTACTTGAAAGCCAGTGTTTCTGATATTTTGTACCCCTACAGAGGGCGACCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.58

Weight (kDa)

9.5

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 26 - 111 7.1e-31 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 370
Acc36I ACCTGC 2 cut(s) 370, 457
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 246
AccII CGCG 1 cut(s) 380
AciI CCGC 3 cut(s) 160, 378, 380
AclWI GGATC 2 cut(s) 5, 261
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 2 cut(s) 97, 569
AfiI CCNNNNNNNGG 3 cut(s) 52, 578, 579
AgsI TTSAA 2 cut(s) 446, 544
AluBI AGCT 2 cut(s) 328, 347
AluI AGCT 2 cut(s) 328, 347
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 1 cut(s) 424
Alw44I GTGCAC 1 cut(s) 357
AlwI GGATC 2 cut(s) 5, 261
AoxI GGCC 2 cut(s) 3, 114
ApaLI GTGCAC 1 cut(s) 357
ApeKI GCWGC 3 cut(s) 144, 199, 202
Asp700I GAANNNNTTC 1 cut(s) 461
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 361
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 95
BauI CACGAG 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 3 cut(s) 131, 211, 214
BcoDI GTCTC 1 cut(s) 424
BfaI CTAG 4 cut(s) 332, 354, 471, 494
BfmI CTRYAG 2 cut(s) 450, 574
BfuAI ACCTGC 2 cut(s) 370, 457
BglII AGATCT 1 cut(s) 490
BisI GCNGC 4 cut(s) 145, 200, 203, 381
BlsI GCNGC 4 cut(s) 146, 201, 204, 382
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 1 cut(s) 97
BmsI GCATC 2 cut(s) 156, 394
BpuEI CTTGAG 1 cut(s) 463
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 2 cut(s) 111, 378
BsaWI WCCGGW 1 cut(s) 52
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 578, 579
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 111, 378
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 3 cut(s) 52, 578, 579
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 549
BseSI GKGCMC 1 cut(s) 361
BseXI GCAGC 3 cut(s) 131, 211, 214
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 2 cut(s) 5, 116
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 361
BsiSI CCGG 2 cut(s) 53, 234
BslI CCNNNNNNNGG 3 cut(s) 52, 578, 579
BsmAI GTCTC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 153
BsnI GGCC 2 cut(s) 5, 116
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 3 cut(s) 10, 253, 490
BspACI CCGC 3 cut(s) 160, 378, 380
BspANI GGCC 2 cut(s) 5, 116
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 454
BspMI ACCTGC 2 cut(s) 370, 457
BspPI GGATC 2 cut(s) 5, 261
BspT107I GGYRCC 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 1 cut(s) 549
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 111, 378
BssMI GATC 3 cut(s) 10, 253, 490
BssSI CACGAG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 408
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 66, 426
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 409
BstFNI CGCG 1 cut(s) 380
BstKTI GATC 3 cut(s) 13, 256, 493
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 3 cut(s) 10, 253, 490
BstMWI GCNNNNNNNGC 3 cut(s) 150, 159, 386
BstSFI CTRYAG 2 cut(s) 450, 574
BstSLI GKGCMC 1 cut(s) 361
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 3 cut(s) 131, 211, 214
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BsuRI GGCC 2 cut(s) 5, 116
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 409
BtsIMutI CAGTG 1 cut(s) 556
BveI ACCTGC 2 cut(s) 370, 457
Cac8I GCNNGC 1 cut(s) 297
CciI TCATGA 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 115
Cfr42I CCGCGG 1 cut(s) 381
Csp6I GTAC 2 cut(s) 96, 568
CviAII CATG 4 cut(s) 87, 212, 386, 520
CviJI RGCY 8 cut(s) 5, 116, 131, 321, 328, 347, 383, 548
CviKI_1 RGCY 8 cut(s) 5, 116, 131, 321, 328, 347, 383, 548
CviQI GTAC 2 cut(s) 96, 568
DdeI CTNAG 2 cut(s) 66, 426
DpnI GATC 3 cut(s) 12, 255, 492
DpnII GATC 3 cut(s) 10, 253, 490
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 424
FaeI CATG 4 cut(s) 90, 215, 389, 523
FaiI YATR 5 cut(s) 88, 213, 225, 387, 521
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 4 cut(s) 86, 211, 385, 519
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 145, 200, 203, 381
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 4 cut(s) 145, 200, 203, 381
FspBI CTAG 4 cut(s) 332, 354, 471, 494
GluI GCNGC 4 cut(s) 145, 200, 203, 381
HaeIII GGCC 2 cut(s) 5, 116
HapII CCGG 2 cut(s) 53, 234
Hin1II CATG 4 cut(s) 90, 215, 389, 523
HincII GTYRAC 1 cut(s) 422
HindII GTYRAC 1 cut(s) 422
HinfI GANTC 2 cut(s) 49, 272
HpaII CCGG 2 cut(s) 53, 234
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 3 cut(s) 247, 359, 422
Hpy188I TCNGA 2 cut(s) 429, 559
Hpy188III TCNNGA 5 cut(s) 212, 264, 410, 466, 480
Hpy8I GTNNAC 3 cut(s) 247, 359, 422
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 64, 70
HpyCH4V TGCA 5 cut(s) 90, 147, 359, 452, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 159, 386
HpyF3I CTNAG 2 cut(s) 66, 426
Hsp92II CATG 4 cut(s) 90, 215, 389, 523
KpnI GGTACC 1 cut(s) 99
KspI CCGCGG 1 cut(s) 381
Kzo9I GATC 3 cut(s) 10, 253, 490
LmnI GCTCC 2 cut(s) 325, 352
Lsp1109I GCAGC 3 cut(s) 131, 211, 214
LweI GCATC 2 cut(s) 156, 394
MaeI CTAG 4 cut(s) 332, 354, 471, 494
MaeIII GTNAC 2 cut(s) 236, 367
MalI GATC 3 cut(s) 12, 255, 492
MbiI CCGCTC 1 cut(s) 162
MboI GATC 3 cut(s) 10, 253, 490
MboII GAAGA 3 cut(s) 158, 267, 526
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 121, 128, 150, 394, 435, 572
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 2 cut(s) 53, 234
Mva1269I GAATGC 1 cut(s) 153
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 3 cut(s) 150, 159, 386
NdeII GATC 3 cut(s) 10, 253, 490
NlaIII CATG 4 cut(s) 90, 215, 389, 523
NlaIV GGNNCC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 367
PagI TCATGA 1 cut(s) 211
PaqCI CACCTGC 1 cut(s) 370
PctI GAATGC 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 49, 272
PkrI GCNGC 4 cut(s) 146, 201, 204, 382
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 490
RsaI GTAC 2 cut(s) 97, 569
RsaNI GTAC 2 cut(s) 96, 568
SacII CCGCGG 1 cut(s) 381
SatI GCNGC 4 cut(s) 145, 200, 203, 381
Sau3AI GATC 3 cut(s) 10, 253, 490
Sau96I GGNCC 1 cut(s) 115
SbfI CCTGCAGG 1 cut(s) 454
SdaI CCTGCAGG 1 cut(s) 454
SduI GDGCHC 1 cut(s) 361
SetI ASST 9 cut(s) 97, 310, 330, 349, 364, 427, 436, 451, 476
SfaNI GCATC 2 cut(s) 156, 394
SfcI CTRYAG 2 cut(s) 450, 574
Sfr303I CCGCGG 1 cut(s) 381
SgrBI CCGCGG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse8387I CCTGCAGG 1 cut(s) 454
Sse9I AATT 1 cut(s) 302
SsiI CCGC 3 cut(s) 160, 378, 380
SspMI CTAG 4 cut(s) 332, 354, 471, 494
TaqII GACCGA 1 cut(s) 232
TasI AATT 1 cut(s) 302
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 2 cut(s) 49, 272
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 144, 199, 202
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 200
TspRI CASTG 1 cut(s) 556
VneI GTGCAC 1 cut(s) 357
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 4 cut(s) 332, 354, 471, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.