RLG00000013996

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003134
Physical Location & Seq
Forward (+)
45293808 .. 45298788
4981 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013996

Sequence Viewer

Length: 591 bp
ATGGATAAGGAAGGCGAAGACGGAGACGAACGATTTCAAAACCAAAACCCAAACCCTAATCAGAGCAGAGGTTCAACGAGGGGAAGATCCTGCAAGGGTTGCCTCTATTACACTTCTCTACACAAATCCAAATCCAAGTACCCCACCTGCGTTGGCTTCTACAGAACTCTCAATCAAGTACCCAGCTACATTGTGGGAGAAACGGAGTTGGAAGCTTCAAAAGCCGATCGCAGCCTTACGGATTTTAAGTACGGGTGTGTAGGTTACTCGGTGTTCTTAGACAGGAAAGATTCAAGTGATCCACATAAAAAGCAGGCGGAATTGCCATTTTGTGTTGGTCTTGAGATATTATATGACGAAAGACCCGCAGACCAGGCTCATGCTCCTACTCCTGCTCATAAAAGTGGAGACAATGTTCGACCTCTACCTCAACCTCGAAGCTATAAACCACATTCAACCGGAGATGAGTACCTGACCAGGTTTAAGAGGAATGCTGGCCTAGTAGCATCAGGTGTTGCTAGGAATTTAAATAGAGTTGGCAACCATATAAAACAGAGCGTGGATAACATCTTATTCGGAAGGTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.97

Weight (kDa)

9.15

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 28 - 117 5.4e-41 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 155
Acc36I ACCTGC 1 cut(s) 155
AciI CCGC 2 cut(s) 317, 366
AclWI GGATC 2 cut(s) 81, 293
AcsI RAATTY 1 cut(s) 523
AfaI GTAC 4 cut(s) 140, 180, 251, 470
AgsI TTSAA 5 cut(s) 38, 75, 219, 294, 456
AjnI CCWGG 2 cut(s) 372, 476
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
AluBI AGCT 3 cut(s) 186, 215, 441
AluI AGCT 3 cut(s) 186, 215, 441
Alw26I GTCTC 2 cut(s) 18, 402
AlwI GGATC 2 cut(s) 81, 293
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 1 cut(s) 523
Asp700I GAANNNNTTC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 582
AvaII GGWCC 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 243
BciT130I CCWGG 2 cut(s) 374, 478
BcoDI GTCTC 2 cut(s) 18, 402
BfaI CTAG 2 cut(s) 500, 519
BfmI CTRYAG 1 cut(s) 160
BfuAI ACCTGC 1 cut(s) 155
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
Bme1390I CCNGG 2 cut(s) 374, 478
Bme18I GGWCC 1 cut(s) 582
BmgT120I GGNCC 1 cut(s) 582
BmrFI CCNGG 2 cut(s) 374, 478
BmsI GCATC 1 cut(s) 515
BpiI GAAGAC 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
BseBI CCWGG 2 cut(s) 374, 478
BseXI GCAGC 1 cut(s) 243
BseYI CCCAGC 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 1 cut(s) 498
BsiEI CGRYCG 1 cut(s) 229
BsiSI CCGG 1 cut(s) 459
BsmAI GTCTC 2 cut(s) 18, 402
BsmBI CGTCTC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 1 cut(s) 498
Bsp143I GATC 3 cut(s) 86, 226, 298
BspACI CCGC 2 cut(s) 317, 366
BspANI GGCC 1 cut(s) 498
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 2 cut(s) 81, 293
BssMI GATC 3 cut(s) 86, 226, 298
Bst2UI CCWGG 2 cut(s) 374, 478
BstAPI GCANNNNNTGC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 315, 496
BstDEI CTNAG 1 cut(s) 277
BstKTI GATC 3 cut(s) 89, 229, 301
BstMAI GTCTC 2 cut(s) 18, 402
BstMBI GATC 3 cut(s) 86, 226, 298
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 3 cut(s) 99, 221, 374
BstNI CCWGG 2 cut(s) 374, 478
BstSCI CCNGG 2 cut(s) 372, 476
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
BsuRI GGCC 1 cut(s) 498
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 2 cut(s) 315, 496
Cfr13I GGNCC 1 cut(s) 582
CsiI ACCWGGT 1 cut(s) 476
Csp6I GTAC 4 cut(s) 139, 179, 250, 469
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 1 cut(s) 380
CviJI RGCY 8 cut(s) 156, 186, 215, 224, 234, 377, 441, 498
CviKI_1 RGCY 8 cut(s) 156, 186, 215, 224, 234, 377, 441, 498
CviQI GTAC 4 cut(s) 139, 179, 250, 469
DdeI CTNAG 1 cut(s) 277
DpnI GATC 3 cut(s) 88, 228, 300
DpnII GATC 3 cut(s) 86, 226, 298
DraI TTTAAA 1 cut(s) 528
EciI GGCGGA 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 582
EcoRII CCWGG 2 cut(s) 372, 476
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 1 cut(s) 383
FaiI YATR 8 cut(s) 306, 352, 354, 381, 399, 444, 546, 548
FatI CATG 1 cut(s) 379
FauI CCCGC 1 cut(s) 373
Fnu4HI GCNGC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 2 cut(s) 500, 519
GluI GCNGC 1 cut(s) 232
GsaI CCCAGC 1 cut(s) 186
HaeIII GGCC 1 cut(s) 498
HapII CCGG 1 cut(s) 459
Hin1II CATG 1 cut(s) 383
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 1 cut(s) 290
HpaII CCGG 1 cut(s) 459
Hpy188I TCNGA 2 cut(s) 63, 578
Hpy188III TCNNGA 1 cut(s) 341
HpyAV CCTTC 2 cut(s) 5, 573
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 221, 374
HpyF3I CTNAG 1 cut(s) 277
Hsp92II CATG 1 cut(s) 383
Kzo9I GATC 3 cut(s) 86, 226, 298
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 1 cut(s) 243
LweI GCATC 1 cut(s) 515
MabI ACCWGGT 1 cut(s) 476
MaeI CTAG 2 cut(s) 500, 519
MaeIII GTNAC 1 cut(s) 263
MalI GATC 3 cut(s) 88, 228, 300
MboI GATC 3 cut(s) 86, 226, 298
MboII GAAGA 2 cut(s) 29, 96
MflI RGATCY 1 cut(s) 86
MluCI AATT 2 cut(s) 320, 523
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 7 cut(s) 62, 72, 113, 432, 438, 444, 480
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 3 cut(s) 246, 483, 527
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 2 cut(s) 374, 478
Mva1269I GAATGC 1 cut(s) 496
MvaI CCWGG 2 cut(s) 374, 478
MwoI GCNNNNNNNGC 3 cut(s) 99, 221, 374
NdeII GATC 3 cut(s) 86, 226, 298
NlaIII CATG 1 cut(s) 383
PaqCI CACCTGC 1 cut(s) 155
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 290
PkrI GCNGC 1 cut(s) 233
Ple19I CGATCG 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 372, 476
PspFI CCCAGC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 372, 476
PspPI GGNCC 1 cut(s) 582
PsuI RGATCY 1 cut(s) 86
PvuI CGATCG 1 cut(s) 229
RsaI GTAC 4 cut(s) 140, 180, 251, 470
RsaNI GTAC 4 cut(s) 139, 179, 250, 469
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 3 cut(s) 246, 483, 527
SatI GCNGC 1 cut(s) 232
Sau3AI GATC 3 cut(s) 86, 226, 298
Sau96I GGNCC 1 cut(s) 582
ScrFI CCNGG 2 cut(s) 374, 478
SexAI ACCWGGT 1 cut(s) 476
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 582
SmiI ATTTAAAT 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 2 cut(s) 320, 523
SsiI CCGC 2 cut(s) 317, 366
SspMI CTAG 2 cut(s) 500, 519
StyD4I CCNGG 2 cut(s) 372, 476
SwaI ATTTAAAT 1 cut(s) 528
TaqI TCGA 2 cut(s) 418, 436
TasI AATT 2 cut(s) 320, 523
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 3 cut(s) 246, 483, 527
Tru9I TTAA 3 cut(s) 246, 483, 527
TseI GCWGC 1 cut(s) 231
TspGWI ACGGA 3 cut(s) 36, 218, 254
VpaK11BI GGWCC 1 cut(s) 582
XapI RAATTY 1 cut(s) 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 2 cut(s) 500, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.