Rh6DG140600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003134
Physical Location & Seq
Reverse (-)
20193002 .. 20198018
5017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG140600.1

Sequence Viewer

Length: 597 bp
ATGGGTAAGGAAGGCGAAGACGGAGACGAACGATTTCAAAACCAAAACCCTAACCCTAACCCTAATCAGAGCAGAGGTTCAACAAGGGGAAGATCCTGCAAGGGTTGCCTCTATTACTCTTCTCTACACAAATCCAAATCCAAGTACCCCACCTGCGTTGGCTTCTACAGAACTCTCAATCAAGTACCCAGCTACATTGTGGGAGAAACGGAGTTGGAAGCTTCAAAAGCCGATCGCAGCCTTACGGATTTTAAGTACGGGTGTGTAGGTTACTCGGTGTTCTTAGACAGGAAAGATTCAAGTGATCCACATAAAAAACAGGCGGAATTGCCATTTTGTGTTGGTCTTGAGATATTATATGACAAAAGACCCGCAGACCAGGCTCATGCTCCTACTCCTGCTCATAAAAGTGGAGACAATGTTCGACCTCTACCTCAACCTCGAAGCTATAAACCACATTCAACCGGAGATGAGTACCTGACCAGGTTTAAGAGGAATGCTGGCCTAGTAGCATCAGGCGTTGCTAGGAATTTAAACAGAGTAGGCAACCATATAAAACAGAGCGTGGATAACATCTTATTCGGAAGGTCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.11

Weight (kDa)

9.41

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 30 - 119 1.3e-41 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 161
AciI CCGC 2 cut(s) 323, 372
AclWI GGATC 2 cut(s) 87, 299
AcsI RAATTY 1 cut(s) 529
AfaI GTAC 4 cut(s) 146, 186, 257, 476
AgsI TTSAA 5 cut(s) 38, 81, 225, 300, 462
AjnI CCWGG 2 cut(s) 378, 482
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
AluBI AGCT 3 cut(s) 192, 221, 447
AluI AGCT 3 cut(s) 192, 221, 447
Alw26I GTCTC 2 cut(s) 18, 408
AlwI GGATC 2 cut(s) 87, 299
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 529
Asp700I GAANNNNTTC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 588
AvaII GGWCC 1 cut(s) 588
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 249
BciT130I CCWGG 2 cut(s) 380, 484
BcoDI GTCTC 2 cut(s) 18, 408
BfaI CTAG 2 cut(s) 506, 525
BfmI CTRYAG 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 380, 484
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 588
BmrFI CCNGG 2 cut(s) 380, 484
BmsI GCATC 1 cut(s) 521
BpiI GAAGAC 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
BseBI CCWGG 2 cut(s) 380, 484
BseXI GCAGC 1 cut(s) 249
BseYI CCCAGC 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 235
BsiSI CCGG 1 cut(s) 465
BsmAI GTCTC 2 cut(s) 18, 408
BsmBI CGTCTC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 1 cut(s) 504
Bsp143I GATC 3 cut(s) 92, 232, 304
BspACI CCGC 2 cut(s) 323, 372
BspANI GGCC 1 cut(s) 504
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 2 cut(s) 87, 299
BssMI GATC 3 cut(s) 92, 232, 304
Bst2UI CCWGG 2 cut(s) 380, 484
Bst6I CTCTTC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 1 cut(s) 283
BstKTI GATC 3 cut(s) 95, 235, 307
BstMAI GTCTC 2 cut(s) 18, 408
BstMBI GATC 3 cut(s) 92, 232, 304
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 105, 227, 380
BstNI CCWGG 2 cut(s) 380, 484
BstSCI CCNGG 2 cut(s) 378, 482
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 1 cut(s) 504
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 588
CsiI ACCWGGT 1 cut(s) 482
Csp6I GTAC 4 cut(s) 145, 185, 256, 475
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviAII CATG 1 cut(s) 386
CviJI RGCY 8 cut(s) 162, 192, 221, 230, 240, 383, 447, 504
CviKI_1 RGCY 8 cut(s) 162, 192, 221, 230, 240, 383, 447, 504
CviQI GTAC 4 cut(s) 145, 185, 256, 475
DdeI CTNAG 1 cut(s) 283
DpnI GATC 3 cut(s) 94, 234, 306
DpnII GATC 3 cut(s) 92, 232, 304
DraI TTTAAA 1 cut(s) 534
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
EciI GGCGGA 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 588
EcoRII CCWGG 2 cut(s) 378, 482
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 1 cut(s) 389
FaiI YATR 8 cut(s) 312, 358, 360, 387, 405, 450, 552, 554
FatI CATG 1 cut(s) 385
FauI CCCGC 1 cut(s) 379
Fnu4HI GCNGC 1 cut(s) 238
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 2 cut(s) 506, 525
GluI GCNGC 1 cut(s) 238
GsaI CCCAGC 1 cut(s) 192
HaeIII GGCC 1 cut(s) 504
HapII CCGG 1 cut(s) 465
Hin1II CATG 1 cut(s) 389
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 1 cut(s) 296
HpaII CCGG 1 cut(s) 465
Hpy188I TCNGA 2 cut(s) 69, 584
Hpy188III TCNNGA 1 cut(s) 347
HpyAV CCTTC 2 cut(s) 5, 579
HpyCH4V TGCA 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 227, 380
HpyF3I CTNAG 1 cut(s) 283
Hsp92II CATG 1 cut(s) 389
Kzo9I GATC 3 cut(s) 92, 232, 304
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 1 cut(s) 249
LweI GCATC 1 cut(s) 521
MabI ACCWGGT 1 cut(s) 482
MaeI CTAG 2 cut(s) 506, 525
MaeIII GTNAC 1 cut(s) 269
MalI GATC 3 cut(s) 94, 234, 306
MboI GATC 3 cut(s) 92, 232, 304
MboII GAAGA 3 cut(s) 29, 102, 111
MflI RGATCY 1 cut(s) 92
MluCI AATT 2 cut(s) 326, 529
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 6 cut(s) 68, 119, 438, 444, 450, 486
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 3 cut(s) 252, 489, 533
MslI CAYNNNNRTG 1 cut(s) 408
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 2 cut(s) 380, 484
Mva1269I GAATGC 1 cut(s) 502
MvaI CCWGG 2 cut(s) 380, 484
MwoI GCNNNNNNNGC 3 cut(s) 105, 227, 380
NdeII GATC 3 cut(s) 92, 232, 304
NlaIII CATG 1 cut(s) 389
PaqCI CACCTGC 1 cut(s) 161
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 239
Ple19I CGATCG 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 378, 482
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 2 cut(s) 378, 482
PspPI GGNCC 1 cut(s) 588
PsuI RGATCY 1 cut(s) 92
PvuI CGATCG 1 cut(s) 235
RsaI GTAC 4 cut(s) 146, 186, 257, 476
RsaNI GTAC 4 cut(s) 145, 185, 256, 475
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 3 cut(s) 252, 489, 533
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 3 cut(s) 92, 232, 304
Sau96I GGNCC 1 cut(s) 588
ScrFI CCNGG 2 cut(s) 380, 484
SexAI ACCWGGT 1 cut(s) 482
SfaNI GCATC 1 cut(s) 521
SfcI CTRYAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 2 cut(s) 326, 529
SsiI CCGC 2 cut(s) 323, 372
SspMI CTAG 2 cut(s) 506, 525
StyD4I CCNGG 2 cut(s) 378, 482
TaqI TCGA 2 cut(s) 424, 442
TasI AATT 2 cut(s) 326, 529
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 252, 489, 533
Tru9I TTAA 3 cut(s) 252, 489, 533
TseI GCWGC 1 cut(s) 237
TspGWI ACGGA 3 cut(s) 36, 224, 260
VpaK11BI GGWCC 1 cut(s) 588
XapI RAATTY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 2 cut(s) 506, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.