pycom02g10710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003134
Physical Location & Seq
Forward (+)
7473324 .. 7475723
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10710.1

Sequence Viewer

Length: 666 bp
ATGCTCAACGAGAGAGCATTCCGTGCGAGTAAAAAGCCGTGTGTGAGGGCACCCGAGCAAAATGGGGGTACACAAACGAGAGAGCGACCGCGACACAACGAGAAGAAGACAGTCACAGATGACACAGTGACGACGACAACGACGACGACGACAGCCATGGATACGAACAACAACAGCAAAGATGAGCTCCGGGACCAATCAAAAGCCTCCCAAAAAGGCAGATCATGCAAAGGAACACTCTATTATTCATCCGCCCTCAAATCCAAGGCTTACAGTCCTCTCTGCGTCGGCGTTCCTCGTGCCATCCCCGAAGTGCCTCAGTCAATAGTTTCGGAAACTGAGACGAAAGCTTCTAAAGATGATCGACATCTTACTGAATTTCGCTATGCTTGTGCTGGCTACTCGCTGTACTTGGATCGTATTACGAAAGACCATTCTGGAAAGGAACAACCAGAATTGCCCGTTTGCTATGGTCTTGAGCTTTTGGTTGGCAAAAGAATGGTTCAAAAGACACCTGCTGCTGCTCCTGTTTCTGCCCATGTCCATCACAAACAGGATGTTCATGAAGTCCCTCAGACTCAGAGGCAGCAACCGACTCAGTCTGCAGGAAGTCTTTTCTTCAACAGGTTTACAAGAAACGCATCGATCTTGTTGCTTCAGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.62

Weight (kDa)

9.45

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 72 - 162 2.2e-36 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 523
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 1 cut(s) 49
AccII CGCG 1 cut(s) 91
AciI CCGC 2 cut(s) 89, 252
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 641
AfaI GTAC 2 cut(s) 70, 410
AgsI TTSAA 2 cut(s) 506, 622
AluBI AGCT 3 cut(s) 187, 350, 481
AluI AGCT 3 cut(s) 187, 350, 481
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 1 cut(s) 53
ApeKI GCWGC 3 cut(s) 518, 521, 586
ApoI RAATTY 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 189
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 505, 508, 598
BccI CCATC 2 cut(s) 311, 552
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 1 cut(s) 634
BciVI GTATCC 1 cut(s) 154
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 603
BfuAI ACCTGC 1 cut(s) 523
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 3 cut(s) 519, 522, 587
BlsI GCNGC 3 cut(s) 520, 523, 588
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 2 cut(s) 51, 194
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 644
BsaBI GATNNNNATC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 156, 264
Bse8I GATNNNNATC 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 644
BseDI CCNNGG 2 cut(s) 156, 264
BseGI GGATG 3 cut(s) 248, 303, 562
BseJI GATNNNNATC 1 cut(s) 366
BseMII CTCAG 5 cut(s) 330, 332, 587, 593, 611
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 3 cut(s) 505, 508, 598
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 49
BshVI ATCGAT 1 cut(s) 644
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 190
BslFI GGGAC 2 cut(s) 206, 554
BsmAI GTCTC 1 cut(s) 335
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 206, 554
BsmI GAATGC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 2 cut(s) 52, 189
Bsp143I GATC 4 cut(s) 221, 361, 415, 645
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 2 cut(s) 89, 252
BspCNI CTCAG 5 cut(s) 331, 331, 586, 592, 610
BspDI ATCGAT 1 cut(s) 644
BspFNI CGCG 1 cut(s) 91
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 51, 194
BspMAI CTGCAG 1 cut(s) 607
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 156, 264
BssMI GATC 4 cut(s) 221, 361, 415, 645
BssSI CACGAG 1 cut(s) 297
BssT1I CCWWGG 2 cut(s) 156, 264
Bst2BI CACGAG 1 cut(s) 297
Bst4CI ACNGT 3 cut(s) 112, 127, 275
BstAPI GCANNNNNTGC 2 cut(s) 23, 225
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 5 cut(s) 318, 339, 573, 579, 597
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 3 cut(s) 248, 303, 562
BstFNI CGCG 1 cut(s) 91
BstKTI GATC 4 cut(s) 224, 364, 418, 648
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 4 cut(s) 221, 361, 415, 645
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 2 cut(s) 23, 225
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 603
BstSLI GKGCMC 1 cut(s) 52
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 3 cut(s) 505, 508, 598
BstV2I GAAGAC 1 cut(s) 113
Bsu15I ATCGAT 1 cut(s) 644
BsuI GTATCC 1 cut(s) 154
BsuTUI ATCGAT 1 cut(s) 644
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 3 cut(s) 248, 303, 562
BtsIMutI CAGTG 1 cut(s) 132
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 1 cut(s) 397
CciI TCATGA 1 cut(s) 562
Cfr13I GGNCC 1 cut(s) 193
ClaI ATCGAT 1 cut(s) 644
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 69, 409
CviAII CATG 4 cut(s) 157, 225, 539, 563
CviJI RGCY 8 cut(s) 37, 155, 187, 206, 269, 350, 399, 481
CviKI_1 RGCY 8 cut(s) 37, 155, 187, 206, 269, 350, 399, 481
CviQI GTAC 2 cut(s) 69, 409
DdeI CTNAG 5 cut(s) 318, 339, 573, 579, 597
DpnI GATC 4 cut(s) 223, 363, 417, 647
DpnII GATC 4 cut(s) 221, 361, 415, 645
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 156, 264
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 53
EcoICRI GAGCTC 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 156, 264
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 2 cut(s) 156, 264
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 4 cut(s) 160, 228, 542, 566
FaiI YATR 6 cut(s) 158, 226, 387, 471, 540, 564
FaqI GGGAC 2 cut(s) 206, 554
FatI CATG 4 cut(s) 156, 224, 538, 562
Fnu4HI GCNGC 3 cut(s) 519, 522, 587
FokI GGATG 3 cut(s) 235, 290, 569
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 3 cut(s) 519, 522, 587
GluI GCNGC 3 cut(s) 519, 522, 587
HapII CCGG 1 cut(s) 190
HgaI GACGC 1 cut(s) 274
Hin1II CATG 4 cut(s) 160, 228, 542, 566
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 2 cut(s) 577, 595
HpaII CCGG 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 71, 630
Hpy188I TCNGA 3 cut(s) 334, 576, 582
Hpy188III TCNNGA 3 cut(s) 438, 476, 563
Hpy8I GTNNAC 2 cut(s) 71, 630
Hpy99I CGWCG 5 cut(s) 136, 145, 148, 151, 290
HpyCH4III ACNGT 3 cut(s) 112, 127, 275
HpyCH4V TGCA 2 cut(s) 228, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 225
HpyF3I CTNAG 5 cut(s) 318, 339, 573, 579, 597
Hsp92II CATG 4 cut(s) 160, 228, 542, 566
Kzo9I GATC 4 cut(s) 221, 361, 415, 645
LmnI GCTCC 2 cut(s) 192, 529
Lsp1109I GCAGC 3 cut(s) 505, 508, 598
LweI GCATC 1 cut(s) 650
MaeIII GTNAC 2 cut(s) 112, 127
MalI GATC 4 cut(s) 223, 363, 417, 647
MboI GATC 4 cut(s) 221, 361, 415, 645
MboII GAAGA 3 cut(s) 115, 118, 610
MhlI GDGCHC 2 cut(s) 52, 189
MluCI AATT 2 cut(s) 377, 455
MlyI GAGTC 2 cut(s) 571, 589
MnlI CCTC 8 cut(s) 39, 217, 266, 288, 306, 327, 576, 582
MspI CCGG 1 cut(s) 190
MspR9I CCNGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 17
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 2 cut(s) 23, 225
NciI CCSGG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 156
NdeII GATC 4 cut(s) 221, 361, 415, 645
NlaIII CATG 4 cut(s) 160, 228, 542, 566
NlaIV GGNNCC 2 cut(s) 51, 194
NmuCI GTSAC 2 cut(s) 112, 127
PagI TCATGA 1 cut(s) 562
PaqCI CACCTGC 1 cut(s) 523
PcsI WCGNNNNNNNCGW 3 cut(s) 137, 140, 146
PctI GAATGC 1 cut(s) 17
PflFI GACNNNGTC 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 3 cut(s) 520, 523, 588
PleI GAGTC 2 cut(s) 571, 589
PpsI GAGTC 2 cut(s) 571, 589
Psp124BI GAGCTC 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 51, 194
PspPI GGNCC 1 cut(s) 193
PstI CTGCAG 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 598
RsaI GTAC 2 cut(s) 70, 410
RsaNI GTAC 2 cut(s) 69, 409
SacI GAGCTC 1 cut(s) 189
SatI GCNGC 3 cut(s) 519, 522, 587
Sau3AI GATC 4 cut(s) 221, 361, 415, 645
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 2 cut(s) 571, 589
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 2 cut(s) 52, 189
SetI ASST 5 cut(s) 189, 352, 483, 517, 629
SfaNI GCATC 1 cut(s) 650
SfcI CTRYAG 1 cut(s) 603
SinI GGWCC 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 2 cut(s) 377, 455
SsiI CCGC 2 cut(s) 89, 252
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 156, 264
TaaI ACNGT 3 cut(s) 112, 127, 275
TaqI TCGA 2 cut(s) 364, 644
TasI AATT 2 cut(s) 377, 455
TatI WGTACW 1 cut(s) 408
TscAI CASTG 1 cut(s) 132
TseFI GTSAC 2 cut(s) 112, 127
TseI GCWGC 3 cut(s) 518, 521, 586
Tsp45I GTSAC 2 cut(s) 112, 127
TspDTI ATGAA 3 cut(s) 237, 551, 579
TspGWI ACGGA 1 cut(s) 11
TspRI CASTG 1 cut(s) 132
Tth111I GACNNNGTC 1 cut(s) 598
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.