MD10G1064100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003134
Physical Location & Seq
Forward (+)
8707194 .. 8710482
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1064100.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGATAAGAACGGCGAAGGCGGAGATGGAGAAATCCCAATCCAAAAACCTAACCCCAATCCTATTCTCGGCGGAAGCGGAAGCGGAGGCGGAGGCGGAGGAGGGTCATCGACCAAGGGAAAGTCATGCAAGGGTTGCCTCTATTACTCCTCCACCCAGAAATCCAAATCCAAATACCCCACCTGCGTCGGCTTCTCCAAAACCCTCCAACAAGTTCCGAGCTACATTGTGGGAGAAACTGAATTGGAGGCGTCTAAAGCAGACCGGACACTTGCTGATTTTAAATATGCCTGCGTTGGTTATTCGGTCTTCTTGGACAGAAAAGATACTCCTGCTGATCCACATAATAAACAAGCAGAATTGCCGTTTTGTGTTGGTCTCGAGGTACTGTATGACAAAAAACCTGCAGTCCATACGCATGCTCCGGCTCCTGCTCATAATAGTGAAGAAAACAGTCGGCCTATTCCTCAACCTCGAACCTACAAACCAGCACACTCAGCAGGAGATGAATACCTCCGCAGGTTTAAGAGGAATGCAGTACTAGTAGCATCAGGTGTTGCTAGAAATGTGAATAGAGCGGGCAACTACGTGAAACAGAGTGTGGATGACATTCTATACCCTTACCGTAGAAGGCCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

23.05

Weight (kDa)

9.34

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 39 - 130 7.8e-44 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 191
Acc36I ACCTGC 3 cut(s) 191, 412, 510
AccBSI CCGCTC 1 cut(s) 578
AciI CCGC 8 cut(s) 21, 72, 78, 84, 90, 96, 517, 578
AclWI GGATC 1 cut(s) 332
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 2 cut(s) 387, 540
AfiI CCNNNNNNNGG 1 cut(s) 68
AhlI ACTAGT 1 cut(s) 541
AluBI AGCT 1 cut(s) 222
AluI AGCT 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 2 cut(s) 458, 632
AspS9I GGNCC 1 cut(s) 633
AvaI CYCGRG 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 301
BccI CCATC 1 cut(s) 20
BceAI ACGGC 2 cut(s) 28, 349
BcoDI GTCTC 1 cut(s) 383
BcuI ACTAGT 1 cut(s) 541
BfaI CTAG 2 cut(s) 542, 561
BfmI CTRYAG 1 cut(s) 405
BfuAI ACCTGC 3 cut(s) 191, 412, 510
BmcAI AGTACT 1 cut(s) 540
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 633
BmiI GGNNCC 1 cut(s) 429
BmsI GCATC 1 cut(s) 557
BpiI GAAGAC 1 cut(s) 301
BsaAI YACGTR 1 cut(s) 589
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 264
BsaXI ACNNNNNCTCC 2 cut(s) 406, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 68
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 610
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 510
BseRI GAGGAG 2 cut(s) 114, 139
BshFI GGCC 2 cut(s) 460, 634
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 2 cut(s) 265, 425
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 2 cut(s) 460, 634
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 380
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 8 cut(s) 21, 72, 78, 84, 90, 96, 517, 578
BspANI GGCC 2 cut(s) 460, 634
BspCNI CTCAG 1 cut(s) 509
BspLI GGNNCC 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 409
BspMI ACCTGC 3 cut(s) 191, 412, 510
BspPI GGATC 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 383
BsrBI CCGCTC 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 1 cut(s) 337
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 114
Bst4CI ACNGT 3 cut(s) 390, 455, 626
BstACI GRCGYC 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 135
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 3 cut(s) 292, 420, 580
BstDEI CTNAG 1 cut(s) 496
BstF5I GGATG 1 cut(s) 610
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 3 cut(s) 135, 257, 497
BstNSI RCATGY 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 301
BsuRI GGCC 2 cut(s) 460, 634
BtsCI GGATG 1 cut(s) 610
BveI ACCTGC 3 cut(s) 191, 412, 510
Cac8I GCNNGC 3 cut(s) 292, 420, 580
Cfr13I GGNCC 1 cut(s) 633
CseI GACGC 2 cut(s) 175, 240
Csp6I GTAC 2 cut(s) 386, 539
CviAII CATG 2 cut(s) 126, 419
CviJI RGCY 5 cut(s) 192, 222, 428, 460, 634
CviKI_1 RGCY 5 cut(s) 192, 222, 428, 460, 634
CviQI GTAC 2 cut(s) 386, 539
DdeI CTNAG 1 cut(s) 496
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
DraI TTTAAA 1 cut(s) 283
EciI GGCGGA 4 cut(s) 36, 87, 105, 111
Eco130I CCWWGG 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 2 cut(s) 129, 422
FaiI YATR 8 cut(s) 127, 288, 345, 393, 414, 420, 438, 616
FatI CATG 2 cut(s) 125, 418
FauI CCCGC 1 cut(s) 571
FokI GGATG 1 cut(s) 617
FspBI CTAG 2 cut(s) 542, 561
HaeIII GGCC 2 cut(s) 460, 634
HapII CCGG 2 cut(s) 265, 425
HgaI GACGC 2 cut(s) 175, 240
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 2 cut(s) 129, 422
HpaII CCGG 2 cut(s) 265, 425
Hpy188I TCNGA 1 cut(s) 219
Hpy188III TCNNGA 1 cut(s) 380
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 11, 624
HpyCH4III ACNGT 3 cut(s) 390, 455, 626
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 3 cut(s) 129, 407, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 257, 497
HpyF3I CTNAG 1 cut(s) 496
HpySE526I ACGT 1 cut(s) 588
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 2 cut(s) 129, 422
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 2 cut(s) 427, 433
LweI GCATC 1 cut(s) 557
MaeI CTAG 2 cut(s) 542, 561
MaeII ACGT 1 cut(s) 588
MalI GATC 1 cut(s) 339
MbiI CCGCTC 1 cut(s) 578
MboI GATC 1 cut(s) 337
MboII GAAGA 2 cut(s) 301, 458
MluCI AATT 2 cut(s) 242, 359
MmeI TCCRAC 1 cut(s) 232
MseI TTAA 2 cut(s) 282, 525
MslI CAYNNNNRTG 2 cut(s) 417, 441
MspI CCGG 2 cut(s) 265, 425
Mva1269I GAATGC 1 cut(s) 538
MwoI GCNNNNNNNGC 3 cut(s) 135, 257, 497
NdeII GATC 1 cut(s) 337
NlaIII CATG 2 cut(s) 129, 422
NlaIV GGNNCC 1 cut(s) 429
NmeAIII GCCGAG 1 cut(s) 48
NspI RCATGY 1 cut(s) 422
PaeI GCATGC 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 380
PaqCI CACCTGC 1 cut(s) 191
PctI GAATGC 1 cut(s) 538
Ppu21I YACGTR 1 cut(s) 589
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 1 cut(s) 633
PstI CTGCAG 1 cut(s) 409
RsaI GTAC 2 cut(s) 387, 540
RsaNI GTAC 2 cut(s) 386, 539
RseI CAYNNNNRTG 2 cut(s) 417, 441
SaqAI TTAA 2 cut(s) 282, 525
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 633
ScaI AGTACT 1 cut(s) 540
SfaNI GCATC 1 cut(s) 557
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 380
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 2 cut(s) 417, 441
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SpeI ACTAGT 1 cut(s) 541
SphI GCATGC 1 cut(s) 422
Sse9I AATT 2 cut(s) 242, 359
SsiI CCGC 8 cut(s) 21, 72, 78, 84, 90, 96, 517, 578
SspMI CTAG 2 cut(s) 542, 561
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 390, 455, 626
TaiI ACGT 1 cut(s) 591
TaqI TCGA 3 cut(s) 110, 381, 475
TaqII GACCGA 1 cut(s) 295
TasI AATT 2 cut(s) 242, 359
TatI WGTACW 1 cut(s) 538
Tru1I TTAA 2 cut(s) 282, 525
Tru9I TTAA 2 cut(s) 282, 525
TspDTI ATGAA 1 cut(s) 522
XceI RCATGY 1 cut(s) 422
XhoI CTCGAG 1 cut(s) 380
XspI CTAG 2 cut(s) 542, 561
ZrmI AGTACT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.