MD15G1249400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003134
Physical Location & Seq
Forward (+)
20808415 .. 20811175
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1249400.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGATACGAACAACAATGGCAAAGACGAGCTCCCGGACCAATCAAAAGCCTCCAAAAAAGGCAGATCATGCAAAGGAACACTCTATTATTCCTCCGCCCTCAAATCCAAGGTTTACAATCCTCTCTGCGTCGGCATTCCTCGCGCCATCCCCGAAGTGCCTCAGTCAATAGTTGCAGAAACTGAAATGAAAGCTTCTAAAGATGATCGACATCTTACTGAATTTCGTTATGCTTGTGCTGGTTACTCTGTGTATTTGGATCGAATTACGAAAGACCATTCCAAGGAACAACCAGAATTGCCCGTTTGCTACGGTCTCGAGCTTTTGGTTGGCAAAAGAATGAATCAAAAGACACCTGCTGCCGCTCCTGTTTCTGCCCATGTCCCTCACAAACGGGATGATCATGAAGTCCCTCAGACTCAGAGGCAGCAACCGCCTCAGTCAGCCGGAAGTCTCTTCTTCAACAGGTTTACAAGAAACGCAGATCTTGTTGCTTCAGGGGTGACTAAGAACATACGTAGAGTTGGGAAGTACATAAAAGGAAGTATCTACGAGTTTCTATACTCGTACAGAGGGCGACCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.94

Weight (kDa)

9.57

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 20 - 109 1.4e-37 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 364
Acc36I ACCTGC 1 cut(s) 364
AccBSI CCGCTC 1 cut(s) 365
AccII CGCG 1 cut(s) 144
AciI CCGC 3 cut(s) 96, 363, 434
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 1 cut(s) 480
AfaI GTAC 2 cut(s) 533, 569
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 1 cut(s) 463
AluBI AGCT 3 cut(s) 31, 194, 322
AluI AGCT 3 cut(s) 31, 194, 322
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 320, 458
AlwI GGATC 1 cut(s) 267
AlwNI CAGNNNCTG 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 317
ApeKI GCWGC 2 cut(s) 359, 427
ApoI RAATTY 1 cut(s) 221
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 346, 439
BccI CCATC 1 cut(s) 155
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 320, 458
BfuAI ACCTGC 1 cut(s) 364
BglII AGATCT 1 cut(s) 484
BisI GCNGC 3 cut(s) 360, 363, 428
BlsI GCNGC 3 cut(s) 361, 364, 429
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 35
BsaAI YACGTR 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 2 cut(s) 108, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse8I GATNNNNATC 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 108, 282
BseGI GGATG 2 cut(s) 147, 403
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 4 cut(s) 176, 428, 434, 452
BseXI GCAGC 2 cut(s) 346, 439
Bsh1236I CGCG 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 35, 447
BslFI GGGAC 2 cut(s) 368, 395
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 2 cut(s) 320, 458
BsmFI GGGAC 2 cut(s) 368, 395
BsmI GAATGC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 5 cut(s) 65, 205, 259, 400, 484
BspACI CCGC 3 cut(s) 96, 363, 434
BspCNI CTCAG 4 cut(s) 175, 427, 433, 451
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 108, 282
BssMI GATC 5 cut(s) 65, 205, 259, 400, 484
BssT1I CCWWGG 2 cut(s) 108, 282
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 518
BstDEI CTNAG 5 cut(s) 162, 414, 420, 438, 507
BstF5I GGATG 2 cut(s) 147, 403
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 5 cut(s) 68, 208, 262, 403, 487
BstMAI GTCTC 2 cut(s) 320, 458
BstMBI GATC 5 cut(s) 65, 205, 259, 400, 484
BstMWI GCNNNNNNNGC 3 cut(s) 69, 141, 433
BstSCI CCNGG 1 cut(s) 33
BstSNI TACGTA 1 cut(s) 518
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 346, 439
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BtsCI GGATG 2 cut(s) 147, 403
BveI ACCTGC 1 cut(s) 364
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 532, 568
CviAII CATG 3 cut(s) 69, 380, 404
CviJI RGCY 5 cut(s) 31, 50, 194, 322, 446
CviKI_1 RGCY 5 cut(s) 31, 50, 194, 322, 446
CviQI GTAC 2 cut(s) 532, 568
DdeI CTNAG 5 cut(s) 162, 414, 420, 438, 507
DpnI GATC 5 cut(s) 67, 207, 261, 402, 486
DpnII GATC 5 cut(s) 65, 205, 259, 400, 484
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 518
Eco130I CCWWGG 2 cut(s) 108, 282
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 480
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 31
EcoT14I CCWWGG 2 cut(s) 108, 282
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 108, 282
FaeI CATG 3 cut(s) 72, 383, 407
FaiI YATR 7 cut(s) 70, 231, 381, 405, 515, 536, 562
FaqI GGGAC 2 cut(s) 368, 395
FatI CATG 3 cut(s) 68, 379, 403
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 3 cut(s) 360, 363, 428
FokI GGATG 2 cut(s) 134, 410
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 360, 363, 428
GlaI GCGC 1 cut(s) 145
GluI GCNGC 3 cut(s) 360, 363, 428
HapII CCGG 2 cut(s) 35, 447
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 146
Hin1II CATG 3 cut(s) 72, 383, 407
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 343, 418
HpaII CCGG 2 cut(s) 35, 447
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 2 cut(s) 115, 471
Hpy188I TCNGA 2 cut(s) 417, 423
Hpy188III TCNNGA 2 cut(s) 317, 404
Hpy8I GTNNAC 2 cut(s) 115, 471
Hpy99I CGWCG 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 2 cut(s) 72, 176
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 141, 433
HpyF3I CTNAG 5 cut(s) 162, 414, 420, 438, 507
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 3 cut(s) 72, 383, 407
HspAI GCGC 1 cut(s) 144
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 5 cut(s) 65, 205, 259, 400, 484
LmnI GCTCC 2 cut(s) 36, 370
LpnPI CCDG 8 cut(s) 48, 225, 306, 369, 381, 451, 460, 483
Lsp1109I GCAGC 2 cut(s) 346, 439
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 2 cut(s) 242, 502
MalI GATC 5 cut(s) 67, 207, 261, 402, 486
MbiI CCGCTC 1 cut(s) 365
MboI GATC 5 cut(s) 65, 205, 259, 400, 484
MboII GAAGA 2 cut(s) 448, 451
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 3 cut(s) 221, 264, 296
MlyI GAGTC 1 cut(s) 412
MspI CCGG 2 cut(s) 35, 447
MspR9I CCNGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 135
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 69, 141, 433
NciI CCSGG 1 cut(s) 35
NdeII GATC 5 cut(s) 65, 205, 259, 400, 484
NlaIII CATG 3 cut(s) 72, 383, 407
NmuCI GTSAC 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 317
PagI TCATGA 1 cut(s) 403
PaqCI CACCTGC 1 cut(s) 364
PctI GAATGC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 3 cut(s) 361, 364, 429
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
Ppu21I YACGTR 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 2 cut(s) 533, 569
RsaNI GTAC 2 cut(s) 532, 568
SacI GAGCTC 1 cut(s) 33
SatI GCNGC 3 cut(s) 360, 363, 428
Sau3AI GATC 5 cut(s) 65, 205, 259, 400, 484
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 1 cut(s) 412
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 33, 114, 196, 324, 358, 470, 520
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 1 cut(s) 37
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SnaBI TACGTA 1 cut(s) 518
Sse9I AATT 3 cut(s) 221, 264, 296
SsiI CCGC 3 cut(s) 96, 363, 434
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 2 cut(s) 108, 282
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 1 cut(s) 520
TaqI TCGA 3 cut(s) 208, 262, 318
TasI AATT 3 cut(s) 221, 264, 296
TatI WGTACW 1 cut(s) 531
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 1 cut(s) 343
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 359, 427
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 3 cut(s) 203, 356, 420
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 221
XhoI CTCGAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.