MD02G1135800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003134
Physical Location & Seq
Forward (+)
11289752 .. 11292470
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1135800.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGATACGAACAACAACAGCAAAGACGAGCTCCGGGACCAATCAAAAACCTCCCAAAAAGGCAGATCATGCAAAGGAACACTCTATTATTCATCCGCCCTCAAATCCAAGGCTTACAATCCTCTCTGCGTCGGCGTTCCTCGCTCCATCCCCGAAGTGCCTCAGTCAATAGTTTCGGAAACTGAGACGAAAGCTTCTAAAGATGATCGACATCTTACTGAATTTCGTTATGCTTGTGCTGGTTACTCGCTGTACTTGGATCGAATTACGAAAGACCATTCTGGAAAGGAACAACCAGAATTGCCTGTTTGCTACGGTCTCGAGCTTTTGGTTGGCAAAAGAATGGTTCAAAAGACACCTGCTGCTGCTCCCGTTTCTGCCCATGTCCATCATAAACAGGTTCGTGAAGTCCCTCAGACTCAGAGGCAGCAACCGACTCAGTCTGCAGGAAGTCTTTTCTTCAACAGGTTTACAAGAAACGCAGATCTTGTTGCTTCAGGGGTGACTAAGAACATACGTAAAGTTGGGAACTACATAAAAGGAAGTGTCGATGATTTTCTATACCAGTACAGAGGGCGACCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.9

Weight (kDa)

9.6

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 20 - 110 1.8e-37 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AciI CCGC 1 cut(s) 96
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 1 cut(s) 480
AfaI GTAC 2 cut(s) 254, 569
AgsI TTSAA 2 cut(s) 350, 463
AluBI AGCT 3 cut(s) 31, 194, 325
AluI AGCT 3 cut(s) 31, 194, 325
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 179, 323
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 320
ApeKI GCWGC 3 cut(s) 362, 365, 427
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 514
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 349, 352, 439
BccI CCATC 2 cut(s) 155, 396
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 179, 323
BfmI CTRYAG 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 367
BglII AGATCT 1 cut(s) 484
BisI GCNGC 3 cut(s) 363, 366, 428
BlsI GCNGC 3 cut(s) 364, 367, 429
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 35
BsaAI YACGTR 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 565
Bse8I GATNNNNATC 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 92, 147
BseJI GATNNNNATC 1 cut(s) 210
BseMII CTCAG 5 cut(s) 174, 176, 428, 434, 452
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 3 cut(s) 349, 352, 439
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 1 cut(s) 34
BslFI GGGAC 2 cut(s) 50, 395
BsmAI GTCTC 2 cut(s) 179, 323
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 50, 395
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 4 cut(s) 65, 205, 259, 484
BspACI CCGC 1 cut(s) 96
BspCNI CTCAG 5 cut(s) 175, 175, 427, 433, 451
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 4 cut(s) 65, 205, 259, 484
BssT1I CCWWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 518
BstDEI CTNAG 6 cut(s) 162, 183, 414, 420, 438, 507
BstF5I GGATG 2 cut(s) 92, 147
BstKTI GATC 4 cut(s) 68, 208, 262, 487
BstMAI GTCTC 2 cut(s) 179, 323
BstMBI GATC 4 cut(s) 65, 205, 259, 484
BstMWI GCNNNNNNNGC 2 cut(s) 69, 141
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 444
BstSNI TACGTA 1 cut(s) 518
BstV1I GCAGC 3 cut(s) 349, 352, 439
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
BtsCI GGATG 2 cut(s) 92, 147
BveI ACCTGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 253, 568
CviAII CATG 2 cut(s) 69, 383
CviJI RGCY 4 cut(s) 31, 113, 194, 325
CviKI_1 RGCY 4 cut(s) 31, 113, 194, 325
CviQI GTAC 2 cut(s) 253, 568
DdeI CTNAG 6 cut(s) 162, 183, 414, 420, 438, 507
DpnI GATC 4 cut(s) 67, 207, 261, 486
DpnII GATC 4 cut(s) 65, 205, 259, 484
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 518
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 480
Eco88I CYCGRG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 2 cut(s) 72, 386
FaiI YATR 7 cut(s) 70, 231, 384, 393, 515, 536, 562
FaqI GGGAC 2 cut(s) 50, 395
FatI CATG 2 cut(s) 68, 382
Fnu4HI GCNGC 3 cut(s) 363, 366, 428
FokI GGATG 2 cut(s) 79, 134
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 3 cut(s) 363, 366, 428
GluI GCNGC 3 cut(s) 363, 366, 428
HapII CCGG 1 cut(s) 34
HgaI GACGC 1 cut(s) 118
Hin1II CATG 2 cut(s) 72, 386
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 418, 436
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 1 cut(s) 471
Hpy188I TCNGA 3 cut(s) 178, 417, 423
Hpy188III TCNNGA 3 cut(s) 282, 320, 404
Hpy8I GTNNAC 1 cut(s) 471
Hpy99I CGWCG 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 2 cut(s) 72, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 141
HpyF3I CTNAG 6 cut(s) 162, 183, 414, 420, 438, 507
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 2 cut(s) 72, 386
Kzo9I GATC 4 cut(s) 65, 205, 259, 484
LmnI GCTCC 3 cut(s) 36, 149, 373
Lsp1109I GCAGC 3 cut(s) 349, 352, 439
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 2 cut(s) 242, 502
MalI GATC 4 cut(s) 67, 207, 261, 486
MboI GATC 4 cut(s) 65, 205, 259, 484
MboII GAAGA 1 cut(s) 451
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 3 cut(s) 221, 264, 299
MlyI GAGTC 2 cut(s) 412, 430
MnlI CCTC 8 cut(s) 61, 110, 132, 150, 171, 417, 423, 566
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 69, 141
NciI CCSGG 1 cut(s) 35
NdeII GATC 4 cut(s) 65, 205, 259, 484
NlaIII CATG 2 cut(s) 72, 386
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 320
PaqCI CACCTGC 1 cut(s) 367
PflFI GACNNNGTC 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 3 cut(s) 364, 367, 429
PleI GAGTC 2 cut(s) 412, 430
PpsI GAGTC 2 cut(s) 412, 430
Ppu21I YACGTR 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 484
PsyI GACNNNGTC 1 cut(s) 439
RsaI GTAC 2 cut(s) 254, 569
RsaNI GTAC 2 cut(s) 253, 568
SacI GAGCTC 1 cut(s) 33
SatI GCNGC 3 cut(s) 363, 366, 428
Sau3AI GATC 4 cut(s) 65, 205, 259, 484
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 2 cut(s) 412, 430
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 33
SetI ASST 8 cut(s) 33, 53, 196, 327, 361, 402, 470, 520
SfcI CTRYAG 1 cut(s) 444
Sfr274I CTCGAG 1 cut(s) 320
SinI GGWCC 1 cut(s) 37
SlaI CTCGAG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SnaBI TACGTA 1 cut(s) 518
Sse9I AATT 3 cut(s) 221, 264, 299
SsiI CCGC 1 cut(s) 96
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 520
TaqI TCGA 4 cut(s) 208, 262, 321, 549
TasI AATT 3 cut(s) 221, 264, 299
TatI WGTACW 2 cut(s) 252, 567
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 3 cut(s) 362, 365, 427
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 1 cut(s) 81
Tth111I GACNNNGTC 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 221
XhoI CTCGAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.