Rh2DG138700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003134
Physical Location & Seq
Forward (+)
11578162 .. 11581284
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG138700.1

Sequence Viewer

Length: 537 bp
ATGAATACAAACAGAGACGACGGCAAGAATCCGATAAAAATGTCAAAGAAGGGTAAATCATGCAAAGGCACCCTTTACTATTCCTCCGCCCTCCAATCCAAAGCCAAGAATCCTCGCTGCATCGGCATCCCCCGCACCCTTCCTCAAGTGCCTAACTCAATAGTTGAAGAATCGAAGGCCGATGCTTCTAAAGATGAGCGACATCTTAAAGATTTTTATTATGCCTGTGCCGGGTACTCGGTCTACTTGGATCAAATTGGAAAAGAGTCTTCCGGTGATAAGCGAACGGCAAAAGAATTGCCTGTTTGTGTTGGCCTGGAGTTTTTGGTTGGCAAAAAAGCTCATACTAGTGCACCTGCGGTCGCTCCTACTGCGGCTCATGCTCTCAACAAAGACGATGCTCGCGAAGTCCGTCAACCTCAGAGACCTAAACCCATTCAATCTGCAGGGAGTGATTTCTTGACCAGGCAAGCATATAATTCGAGTATTCAACTCATTAAGTATTGGTCTCTCCGAAACTATATACCTGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

19.71

Weight (kDa)

9.52

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 17 - 109 1e-39 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 364
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 405
AciI CCGC 4 cut(s) 87, 133, 359, 374
AclWI GGATC 1 cut(s) 258
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 231
AgsI TTSAA 3 cut(s) 167, 440, 491
AhlI ACTAGT 1 cut(s) 347
AjnI CCWGG 2 cut(s) 315, 464
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw21I GWGCWC 1 cut(s) 355
Alw26I GTCTC 3 cut(s) 9, 418, 513
Alw44I GTGCAC 1 cut(s) 351
AlwI GGATC 1 cut(s) 258
AoxI GGCC 2 cut(s) 177, 313
ApaLI GTGCAC 1 cut(s) 351
ApeKI GCWGC 1 cut(s) 117
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 355
BbvI GCAGC 1 cut(s) 104
BceAI ACGGC 2 cut(s) 37, 303
BcgI CGANNNNNNTGC 2 cut(s) 462, 496
BciT130I CCWGG 2 cut(s) 317, 466
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 3 cut(s) 9, 418, 513
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 364
BisI GCNGC 2 cut(s) 118, 375
BlsI GCNGC 2 cut(s) 119, 376
Bme1390I CCNGG 3 cut(s) 232, 317, 466
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 3 cut(s) 232, 317, 466
BmsI GCATC 4 cut(s) 129, 135, 172, 388
BpiI GAAGAC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 232
BsaI GGTCTC 2 cut(s) 418, 513
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 231
BseBI CCWGG 2 cut(s) 317, 466
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 2 cut(s) 179, 315
BshNI GGYRCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 355
BsiSI CCGG 2 cut(s) 231, 273
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 3 cut(s) 9, 418, 513
BsmBI CGTCTC 1 cut(s) 9
BsnI GGCC 2 cut(s) 179, 315
Bso31I GGTCTC 2 cut(s) 418, 513
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 1 cut(s) 250
Bsp68I TCGCGA 1 cut(s) 405
BspACI CCGC 4 cut(s) 87, 133, 359, 374
BspANI GGCC 2 cut(s) 179, 315
BspCNI CTCAG 1 cut(s) 433
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 1 cut(s) 70
BspMAI CTGCAG 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 68
BspTNI GGTCTC 2 cut(s) 418, 513
BssMI GATC 1 cut(s) 250
Bst2UI CCWGG 2 cut(s) 317, 466
BstC8I GCNNGC 2 cut(s) 403, 471
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 405
BstKTI GATC 1 cut(s) 253
BstMAI GTCTC 3 cut(s) 9, 418, 513
BstMBI GATC 1 cut(s) 250
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 4 cut(s) 123, 132, 371, 380
BstNI CCWGG 2 cut(s) 317, 466
BstSCI CCNGG 3 cut(s) 230, 315, 464
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 355
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 104
BstV2I GAAGAC 1 cut(s) 261
BsuRI GGCC 2 cut(s) 179, 315
BtsCI GGATG 1 cut(s) 126
BtuMI TCGCGA 1 cut(s) 405
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 2 cut(s) 403, 471
Csp6I GTAC 1 cut(s) 235
CviAII CATG 2 cut(s) 60, 380
CviJI RGCY 5 cut(s) 104, 179, 315, 341, 377
CviKI_1 RGCY 5 cut(s) 104, 179, 315, 341, 377
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 1 cut(s) 420
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
EciI GGCGGA 1 cut(s) 76
Eco31I GGTCTC 2 cut(s) 418, 513
EcoRII CCWGG 2 cut(s) 315, 464
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 2 cut(s) 63, 383
FaiI YATR 8 cut(s) 61, 222, 345, 381, 475, 477, 522, 524
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FatI CATG 2 cut(s) 59, 379
FauI CCCGC 1 cut(s) 140
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 118, 375
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 2 cut(s) 118, 375
FspBI CTAG 1 cut(s) 348
GluI GCNGC 2 cut(s) 118, 375
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 2 cut(s) 179, 315
HapII CCGG 2 cut(s) 231, 273
Hin1II CATG 2 cut(s) 63, 383
HincII GTYRAC 1 cut(s) 416
HindII GTYRAC 1 cut(s) 416
HinfI GANTC 4 cut(s) 28, 109, 170, 266
HpaII CCGG 2 cut(s) 231, 273
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 3 cut(s) 244, 353, 416
Hpy188I TCNGA 3 cut(s) 33, 423, 515
Hpy188III TCNNGA 2 cut(s) 404, 460
Hpy8I GTNNAC 3 cut(s) 244, 353, 416
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 43, 149, 169
HpyCH4V TGCA 4 cut(s) 63, 120, 353, 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 132, 371, 380
HpyF3I CTNAG 1 cut(s) 420
Hsp92II CATG 2 cut(s) 63, 383
Kzo9I GATC 1 cut(s) 250
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 4 cut(s) 129, 135, 172, 388
MaeI CTAG 1 cut(s) 348
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 2 cut(s) 179, 261
MhlI GDGCHC 1 cut(s) 355
MluCI AATT 3 cut(s) 255, 296, 478
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 5 cut(s) 94, 101, 123, 153, 429
MseI TTAA 2 cut(s) 207, 498
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 2 cut(s) 231, 273
MspR9I CCNGG 3 cut(s) 232, 317, 466
MvaI CCWGG 2 cut(s) 317, 466
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 4 cut(s) 123, 132, 371, 380
NciI CCSGG 1 cut(s) 232
NdeII GATC 1 cut(s) 250
NlaIII CATG 2 cut(s) 63, 383
NlaIV GGNNCC 1 cut(s) 70
NruI TCGCGA 1 cut(s) 405
PaqCI CACCTGC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 409
PfeI GAWTC 3 cut(s) 28, 109, 170
PkrI GCNGC 2 cut(s) 119, 376
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
Psp6I CCWGG 2 cut(s) 315, 464
PspGI CCWGG 2 cut(s) 315, 464
PspN4I GGNNCC 1 cut(s) 70
PstI CTGCAG 1 cut(s) 448
RruI TCGCGA 1 cut(s) 405
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 2 cut(s) 207, 498
SatI GCNGC 2 cut(s) 118, 375
Sau3AI GATC 1 cut(s) 250
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 232, 317, 466
SduI GDGCHC 1 cut(s) 355
SetI ASST 5 cut(s) 343, 358, 421, 430, 529
SfaNI GCATC 4 cut(s) 129, 135, 172, 388
SfcI CTRYAG 1 cut(s) 444
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 3 cut(s) 255, 296, 478
SsiI CCGC 4 cut(s) 87, 133, 359, 374
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 3 cut(s) 230, 315, 464
TaqI TCGA 2 cut(s) 173, 482
TaqII GACCGA 1 cut(s) 229
TasI AATT 3 cut(s) 255, 296, 478
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 3 cut(s) 28, 109, 170
Tru1I TTAA 2 cut(s) 207, 498
Tru9I TTAA 2 cut(s) 207, 498
TseI GCWGC 1 cut(s) 117
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 401
VneI GTGCAC 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.