FvH4_1g21842

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000702
Physical Location & Seq
Reverse (-)
13766328 .. 13767886
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21842.t1

Sequence Viewer

Length: 1074 bp
ATGGCAAACTACTCCAGAAGCCTCCTTGTTCTCTCACTTGTCCTTGTGTTCCTGAACTTTAACTCAGCCCTGGGAGCCACTACTGCAGTCACTTACACTGTGACAAGTCTAGGAGCGAACCCTGATGGCAAGACCGACTCGATCAAAGCATTCCACTCTGCATGGGCCAAAGCTTGCTCCTCCGCCAGTCCTGCTGTCATTTATGTCCCGGTGGGGAGGTTCTTGCTTGCCAAAGCTGTCTTCAACGGGCCATGCAAGAACAACGCCATTACATTCCAAATTGCAGGCACGCTCGTTGCCCCATCGGATTATCGGGTAATAGGAAATGCCGGTAACTGGCTTTTCTTTGAACATGTGAACGGGGTCACCATCTCCGGGGGCATACTTGATGGACAAGGCACCGGATTGTGGGACTGCAAGGCCTCTGGCAAGGGTTGCCCCAAAGGAGCAACTACACTTGGTTTTTCCAATTCGAACAACATTGTGGTGAATGGAGTGTCATCCCTAAACAGCCAAATGTTCCACATAGTCATCAACGGCTGCCATAACGTGAAGATGCTTGGTGTTAGGGTTTCTGCCTCTGGCAACAGCCCCAACACCGATGGCATTCACGTTCAAATGTCTACCGGTGTCACTATTCTGAACTCCAAAATTTCGACCGGCGATGACTGTGTCTCAATTGGCCCCGGCACCAGTAATTTGTGGATTGAGAACGTTGCATGTGGACCTGGGCATGGAATTAGCATTGGGAGCTTAGGCAAAGAGCAAGAGGAAGTTGGAGTGCAAAACGTGACAGTTAAAACAGTTACATTCACCGGTACGCAGAACGGCGTGAGGATCAAGTCATGGGGAAGACCCAGTACTGGGTTTGCTAGGAACATTCTCTTTCAACATGCTGTAATGGTCAATGTCAAAAATCCTATTGTTATTGATCAAAATTACTGCCCCGATGAAAAGGGTTGCCCCGGTCAGGAGTCGGGAATTCAAATTAGCGATGTCATGTATCAAGACATTCATGGCACATCAGCAACAGAAGTTGCCGTGAAATTTGACTGCAGCTCCAAGTATCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

358

Amino Acids

37.41

Weight (kDa)

8.29

Isoelectric Point (pI)

28.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 34 - 284 2.1e-12 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 59 - 356 6.8e-82 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 398, 689
AccI GTMKAC 1 cut(s) 623
AciI CCGC 1 cut(s) 183
AclI AACGTT 1 cut(s) 714
AclWI GGATC 1 cut(s) 845
AcsI RAATTY 3 cut(s) 651, 981, 1046
AfaI GTAC 2 cut(s) 820, 862
AfiI CCNNNNNNNGG 7 cut(s) 336, 375, 408, 734, 863, 864, 970
AflIII ACRYGT 1 cut(s) 352
AgeI ACCGGT 2 cut(s) 626, 815
AgsI TTSAA 5 cut(s) 244, 350, 617, 890, 986
AjnI CCWGG 2 cut(s) 69, 727
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 4 cut(s) 173, 236, 753, 1059
AluI AGCT 4 cut(s) 173, 236, 753, 1059
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 1 cut(s) 845
AoxI GGCC 4 cut(s) 165, 248, 420, 682
ApeKI GCWGC 2 cut(s) 540, 1056
ApoI RAATTY 3 cut(s) 651, 981, 1046
AsiGI ACCGGT 2 cut(s) 626, 815
AspS9I GGNCC 4 cut(s) 165, 248, 683, 725
AsuC2I CCSGG 4 cut(s) 209, 376, 687, 966
AsuHPI GGTGA 3 cut(s) 358, 499, 805
AsuII TTCGAA 1 cut(s) 473
AvaII GGWCC 1 cut(s) 725
BanI GGYRCC 2 cut(s) 398, 689
BarI GAAGNNNNNNTAC 2 cut(s) 844, 876
BbsI GAAGAC 2 cut(s) 232, 859
BbvI GCAGC 2 cut(s) 527, 1068
BccI CCATC 5 cut(s) 119, 310, 377, 383, 596
BceAI ACGGC 3 cut(s) 553, 844, 1025
BciT130I CCWGG 2 cut(s) 71, 729
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 4 cut(s) 209, 376, 687, 966
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 2 cut(s) 110, 873
BfmI CTRYAG 2 cut(s) 84, 1054
BisI GCNGC 2 cut(s) 541, 1057
BlsI GCNGC 2 cut(s) 542, 1058
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 6 cut(s) 71, 209, 376, 687, 729, 966
Bme18I GGWCC 1 cut(s) 725
BmgT120I GGNCC 4 cut(s) 165, 248, 683, 725
BmiI GGNNCC 4 cut(s) 76, 400, 685, 691
BmrFI CCNGG 6 cut(s) 71, 209, 376, 687, 729, 966
BmrI ACTGGG 2 cut(s) 852, 873
BmsI GCATC 1 cut(s) 546
BmuI ACTGGG 2 cut(s) 852, 873
BpiI GAAGAC 2 cut(s) 232, 859
Bpu10I CCTNAGC 1 cut(s) 754
Bpu14I TTCGAA 1 cut(s) 473
BpuMI CCSGG 4 cut(s) 209, 376, 687, 966
BsaJI CCNNGG 6 cut(s) 69, 70, 375, 685, 728, 964
BsaWI WCCGGW 3 cut(s) 401, 626, 815
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 7 cut(s) 336, 375, 408, 734, 863, 864, 970
Bse118I RCCGGY 4 cut(s) 329, 626, 659, 815
Bse1I ACTGG 5 cut(s) 186, 341, 693, 858, 868
BseBI CCWGG 2 cut(s) 71, 729
BseDI CCNNGG 6 cut(s) 69, 70, 375, 685, 728, 964
BseGI GGATG 1 cut(s) 500
BseLI CCNNNNNNNGG 7 cut(s) 336, 375, 408, 734, 863, 864, 970
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 5 cut(s) 186, 341, 693, 858, 868
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 2 cut(s) 527, 1068
Bsh1285I CGRYCG 1 cut(s) 660
BshFI GGCC 4 cut(s) 167, 250, 422, 684
BshNI GGYRCC 2 cut(s) 398, 689
BshTI ACCGGT 2 cut(s) 626, 815
BsiEI CGRYCG 1 cut(s) 660
BsiSI CCGG 9 cut(s) 209, 330, 375, 402, 627, 660, 687, 816, 966
BslFI GGGAC 2 cut(s) 191, 425
BslI CCNNNNNNNGG 7 cut(s) 336, 375, 408, 734, 863, 864, 970
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 2 cut(s) 191, 425
BsmI GAATGC 2 cut(s) 149, 606
BsnI GGCC 4 cut(s) 167, 250, 422, 684
Bsp119I TTCGAA 1 cut(s) 473
Bsp143I GATC 3 cut(s) 141, 837, 931
BspACI CCGC 1 cut(s) 183
BspANI GGCC 4 cut(s) 167, 250, 422, 684
BspCNI CTCAG 1 cut(s) 77
BspLI GGNNCC 4 cut(s) 76, 400, 685, 691
BspMAI CTGCAG 2 cut(s) 88, 1058
BspPI GGATC 1 cut(s) 845
BspT104I TTCGAA 1 cut(s) 473
BspT107I GGYRCC 2 cut(s) 398, 689
BsrFI RCCGGY 4 cut(s) 329, 626, 659, 815
BsrI ACTGG 5 cut(s) 186, 341, 693, 858, 868
BssAI RCCGGY 4 cut(s) 329, 626, 659, 815
BssECI CCNNGG 6 cut(s) 69, 70, 375, 685, 728, 964
BssMI GATC 3 cut(s) 141, 837, 931
Bst2UI CCWGG 2 cut(s) 71, 729
Bst4CI ACNGT 4 cut(s) 100, 671, 796, 805
BstAPI GCANNNNNTGC 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 4 cut(s) 175, 228, 286, 290
BstDEI CTNAG 2 cut(s) 64, 754
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 1 cut(s) 500
BstKTI GATC 3 cut(s) 144, 840, 934
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 3 cut(s) 141, 837, 931
BstMCI CGRYCG 1 cut(s) 660
BstMWI GCNNNNNNNGC 5 cut(s) 74, 83, 191, 435, 750
BstNI CCWGG 2 cut(s) 71, 729
BstNSI RCATGY 3 cut(s) 356, 723, 896
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 6 cut(s) 69, 207, 374, 685, 727, 964
BstSFI CTRYAG 2 cut(s) 84, 1054
BstV1I GCAGC 2 cut(s) 527, 1068
BstV2I GAAGAC 2 cut(s) 232, 859
BsuRI GGCC 4 cut(s) 167, 250, 422, 684
BtgZI GCGATG 2 cut(s) 678, 1008
BtsCI GGATG 1 cut(s) 500
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 175, 228, 286, 290
Cfr10I RCCGGY 4 cut(s) 329, 626, 659, 815
Cfr13I GGNCC 4 cut(s) 165, 248, 683, 725
Csp6I GTAC 2 cut(s) 819, 861
CspAI ACCGGT 2 cut(s) 626, 815
CviAII CATG 9 cut(s) 162, 252, 353, 720, 734, 846, 893, 1000, 1016
CviQI GTAC 2 cut(s) 819, 861
DdeI CTNAG 2 cut(s) 64, 754
DpnI GATC 3 cut(s) 143, 839, 933
DpnII GATC 3 cut(s) 141, 837, 931
EciI GGCGGA 1 cut(s) 172
Eco147I AGGCCT 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 725
Eco91I GGTNACC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 981
EcoRII CCWGG 2 cut(s) 69, 727
FaeI CATG 9 cut(s) 165, 255, 356, 723, 737, 849, 896, 1003, 1019
FaqI GGGAC 2 cut(s) 191, 425
FatI CATG 9 cut(s) 161, 251, 352, 719, 733, 845, 892, 999, 1015
FbaI TGATCA 1 cut(s) 931
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 2 cut(s) 541, 1057
FokI GGATG 1 cut(s) 487
Fsp4HI GCNGC 2 cut(s) 541, 1057
FspBI CTAG 2 cut(s) 110, 873
GluI GCNGC 2 cut(s) 541, 1057
HaeIII GGCC 4 cut(s) 167, 250, 422, 684
HapII CCGG 9 cut(s) 209, 330, 375, 402, 627, 660, 687, 816, 966
Hin1II CATG 9 cut(s) 165, 255, 356, 723, 737, 849, 896, 1003, 1019
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 2 cut(s) 137, 974
HpaII CCGG 9 cut(s) 209, 330, 375, 402, 627, 660, 687, 816, 966
HphI GGTGA 3 cut(s) 358, 499, 805
Hpy166II GTNNAC 3 cut(s) 358, 624, 725
Hpy188I TCNGA 2 cut(s) 307, 642
Hpy188III TCNNGA 5 cut(s) 15, 52, 971, 978, 1007
Hpy8I GTNNAC 3 cut(s) 358, 624, 725
HpyCH4III ACNGT 4 cut(s) 100, 671, 796, 805
HpyCH4IV ACGT 4 cut(s) 549, 612, 714, 789
HpyCH4V TGCA 8 cut(s) 86, 161, 255, 284, 417, 719, 784, 1056
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 83, 191, 435, 750
HpyF3I CTNAG 2 cut(s) 64, 754
HpySE526I ACGT 4 cut(s) 549, 612, 714, 789
Hsp92II CATG 9 cut(s) 165, 255, 356, 723, 737, 849, 896, 1003, 1019
Ksp22I TGATCA 1 cut(s) 931
Kzo9I GATC 3 cut(s) 141, 837, 931
LmnI GCTCC 6 cut(s) 74, 113, 182, 446, 750, 1064
Lsp1109I GCAGC 2 cut(s) 527, 1068
LweI GCATC 1 cut(s) 546
MaeI CTAG 2 cut(s) 110, 873
MaeII ACGT 4 cut(s) 549, 612, 714, 789
MaeIII GTNAC 7 cut(s) 88, 100, 332, 364, 631, 790, 805
MalI GATC 3 cut(s) 143, 839, 933
MboI GATC 3 cut(s) 141, 837, 931
MboII GAAGA 3 cut(s) 232, 565, 864
MfeI CAATTG 1 cut(s) 678
MlyI GAGTC 2 cut(s) 131, 983
MmeI TCCRAC 1 cut(s) 757
MnlI CCTC 7 cut(s) 32, 190, 210, 433, 589, 763, 828
MseI TTAA 2 cut(s) 60, 798
MslI CAYNNNNRTG 1 cut(s) 485
MspI CCGG 9 cut(s) 209, 330, 375, 402, 627, 660, 687, 816, 966
MspR9I CCNGG 6 cut(s) 71, 209, 376, 687, 729, 966
MunI CAATTG 1 cut(s) 678
Mva1269I GAATGC 2 cut(s) 149, 606
MvaI CCWGG 2 cut(s) 71, 729
MwoI GCNNNNNNNGC 5 cut(s) 74, 83, 191, 435, 750
NciI CCSGG 4 cut(s) 209, 376, 687, 966
NdeII GATC 3 cut(s) 141, 837, 931
NlaIII CATG 9 cut(s) 165, 255, 356, 723, 737, 849, 896, 1003, 1019
NlaIV GGNNCC 4 cut(s) 76, 400, 685, 691
NmuCI GTSAC 5 cut(s) 88, 100, 364, 631, 790
NspI RCATGY 3 cut(s) 356, 723, 896
NspV TTCGAA 1 cut(s) 473
PasI CCCWGGG 1 cut(s) 70
PceI AGGCCT 1 cut(s) 422
PciI ACATGT 1 cut(s) 352
PctI GAATGC 2 cut(s) 149, 606
PflFI GACNNNGTC 1 cut(s) 671
PinAI ACCGGT 2 cut(s) 626, 815
PkrI GCNGC 2 cut(s) 542, 1058
PleI GAGTC 2 cut(s) 131, 982
PpsI GAGTC 2 cut(s) 131, 982
PscI ACATGT 1 cut(s) 352
Psp1406I AACGTT 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 69, 727
PspEI GGTNACC 1 cut(s) 364
PspGI CCWGG 2 cut(s) 69, 727
PspN4I GGNNCC 4 cut(s) 76, 400, 685, 691
PspPI GGNCC 4 cut(s) 165, 248, 683, 725
PstI CTGCAG 2 cut(s) 88, 1058
PsyI GACNNNGTC 1 cut(s) 671
RsaI GTAC 2 cut(s) 820, 862
RsaNI GTAC 2 cut(s) 819, 861
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 2 cut(s) 60, 798
SatI GCNGC 2 cut(s) 541, 1057
Sau3AI GATC 3 cut(s) 141, 837, 931
Sau96I GGNCC 4 cut(s) 165, 248, 683, 725
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 2 cut(s) 131, 983
ScrFI CCNGG 6 cut(s) 71, 209, 376, 687, 729, 966
SfaNI GCATC 1 cut(s) 546
SfcI CTRYAG 2 cut(s) 84, 1054
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 485
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 1 cut(s) 183
SspMI CTAG 2 cut(s) 110, 873
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 6 cut(s) 69, 207, 374, 685, 727, 964
TaaI ACNGT 4 cut(s) 100, 671, 796, 805
TaiI ACGT 4 cut(s) 552, 615, 717, 792
TaqI TCGA 3 cut(s) 140, 473, 656
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 1 cut(s) 860
Tru1I TTAA 2 cut(s) 60, 798
Tru9I TTAA 2 cut(s) 60, 798
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 5 cut(s) 88, 100, 364, 631, 790
TseI GCWGC 2 cut(s) 540, 1056
Tsp45I GTSAC 5 cut(s) 88, 100, 364, 631, 790
TspDTI ATGAA 2 cut(s) 966, 1004
TspGWI ACGGA 1 cut(s) 1059
TspRI CASTG 1 cut(s) 103
Tth111I GACNNNGTC 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 725
XapI RAATTY 3 cut(s) 651, 981, 1046
XceI RCATGY 3 cut(s) 356, 723, 896
XmiI GTMKAC 1 cut(s) 623
XspI CTAG 2 cut(s) 110, 873
ZrmI AGTACT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.