RLG00000018208

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000702
Physical Location & Seq
Reverse (-)
26886661 .. 26888162
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018208

Sequence Viewer

Length: 1026 bp
ATGAATGTCAACTTAGCCTTAGGCGCAACTGCTTCAGTCACTTACACTGTGACAAGTCTGGGAGCGAATCCTGATGGCAAGACCGACTCAACCAAAGCATTCCTCTCTGCATGGGCCAAAGCTTGTGTCTCTGCCAAGCCTACTGTCATTTATGTCCCGGCAGGAGATTACTGGGTGATAGGAAATGCCGGTAGCTGGCTTTTCTTTGAGCATGTGAATGGGGTTACCATCTCCGGTGGTATTCTTGACGGACAAGGCACTGGACTGTGGGACTGCAAGGACTCCGGCAAGGGTTGCCCCAAAGGAGCGACTACACTTGGTTTTTCCAATTCGAACAACATTGTGATGAATGGAGTGTCATCCCTAAACAGCCAAATGTTTCACATAGTGATCAACGGCTGCCACAACGTGAAAATGCTAGGTGTTAGGGTTTCTGCCTCTGGCAACAGCCGCAACACAGATGGCATTCACGTTCAAATGTCTACCAGTGTCACTATTCTCAACTCCAAAATTGCGAGGGGAGATGATTGTGTTTCAATTGGCCTCGGCACCAGTAATTTGTGGATTGAAAACGTTGCATGTGGTCCTGGGCATGGAATTAGCATTGGGAGTTTAGGCAAGGAGCAAGAGGAAGTGGGAGTGCAAAACGTAACGGTTAAAACAGTTACATTCACCGGTACTCAGAATGGTACGCAGTACTGGTTTGCCAGGAACATTCTTTTTCAACATGCTGTAATGGTCAATGTCAAAAATCCTATTGTTATAGATCAAAATTACTGCCCCGACGAAAAGCGTTGCCCTGGTCAGGAGTCTGGAGTTCAAATTAGCGACATTATGTACCAAGACATTCACGGCACATCGGCAACAGAAGTTGCTGTGAAATTCGATTGCAGCTCCAAATATCCTTGTAACAAAATCACATTGGAGGATGTTAAGCTCACTTACAAGAACCAAGCAGCTGAAGCTTCTTGTAGCCATGCAGGTGGAACAGCTGAGGGTACGGTTCAACCTACAAGTTGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

342

Amino Acids

35.96

Weight (kDa)

6.23

Isoelectric Point (pI)

28.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 17 - 244 7.9e-12 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 64 - 325 2.4e-68 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 971
Acc36I ACCTGC 1 cut(s) 971
AccB1I GGYRCC 1 cut(s) 548
AccI GTMKAC 1 cut(s) 482
AciI CCGC 1 cut(s) 451
AclI AACGTT 1 cut(s) 573
AcsI RAATTY 1 cut(s) 881
AcuI CTGAAG 2 cut(s) 18, 981
AdeI CACNNNGTG 2 cut(s) 388, 409
AfaI GTAC 5 cut(s) 679, 691, 698, 839, 1000
AfiI CCNNNNNNNGG 3 cut(s) 195, 593, 805
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 6 cut(s) 476, 537, 569, 725, 821, 1007
AjnI CCWGG 3 cut(s) 586, 707, 799
AluBI AGCT 7 cut(s) 122, 195, 894, 937, 959, 965, 992
AluI AGCT 7 cut(s) 122, 195, 894, 937, 959, 965, 992
Alw26I GTCTC 1 cut(s) 133
AoxI GGCC 2 cut(s) 114, 541
ApeKI GCWGC 3 cut(s) 399, 891, 956
ApoI RAATTY 1 cut(s) 881
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AsiGI ACCGGT 1 cut(s) 674
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 114, 584
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 187, 664
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 1 cut(s) 584
AxyI CCTNAGG 1 cut(s) 19
BanI GGYRCC 1 cut(s) 548
BbvCI CCTCAGC 1 cut(s) 993
BbvI GCAGC 3 cut(s) 386, 903, 968
BccI CCATC 3 cut(s) 68, 236, 455
BceAI ACGGC 2 cut(s) 412, 868
BciT130I CCWGG 3 cut(s) 588, 709, 801
BclI TGATCA 1 cut(s) 390
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 1022
BfuAI ACCTGC 1 cut(s) 971
BisI GCNGC 4 cut(s) 400, 451, 892, 957
BlsI GCNGC 4 cut(s) 401, 452, 893, 958
BmcAI AGTACT 1 cut(s) 698
Bme1390I CCNGG 4 cut(s) 158, 588, 709, 801
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 2 cut(s) 114, 584
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 4 cut(s) 158, 588, 709, 801
BmrI ACTGGG 1 cut(s) 181
BmuI ACTGGG 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 834
Bpu10I CCTNAGC 1 cut(s) 993
Bpu14I TTCGAA 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 158
BsaJI CCNNGG 3 cut(s) 544, 587, 799
BsaWI WCCGGW 2 cut(s) 233, 674
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 593, 805
Bse118I RCCGGY 2 cut(s) 188, 674
Bse1I ACTGG 5 cut(s) 176, 265, 486, 552, 704
Bse21I CCTNAGG 1 cut(s) 19
BseBI CCWGG 3 cut(s) 588, 709, 801
BseDI CCNNGG 3 cut(s) 544, 587, 799
BseGI GGATG 2 cut(s) 359, 934
BseLI CCNNNNNNNGG 3 cut(s) 195, 593, 805
BseMII CTCAG 2 cut(s) 695, 984
BseNI ACTGG 5 cut(s) 176, 265, 486, 552, 704
BseXI GCAGC 3 cut(s) 386, 903, 968
BshFI GGCC 2 cut(s) 116, 543
BshNI GGYRCC 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 674
BsiSI CCGG 5 cut(s) 158, 189, 234, 285, 675
BslFI GGGAC 2 cut(s) 140, 284
BslI CCNNNNNNNGG 3 cut(s) 195, 593, 805
BsmAI GTCTC 1 cut(s) 133
BsmFI GGGAC 2 cut(s) 140, 284
BsmI GAATGC 2 cut(s) 98, 465
BsnI GGCC 2 cut(s) 116, 543
Bsp119I TTCGAA 1 cut(s) 332
Bsp143I GATC 2 cut(s) 390, 766
BspACI CCGC 1 cut(s) 451
BspANI GGCC 2 cut(s) 116, 543
BspCNI CTCAG 2 cut(s) 694, 985
BspLI GGNNCC 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 971
BspT104I TTCGAA 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 548
BsrFI RCCGGY 2 cut(s) 188, 674
BsrI ACTGG 5 cut(s) 176, 265, 486, 552, 704
BssAI RCCGGY 2 cut(s) 188, 674
BssECI CCNNGG 3 cut(s) 544, 587, 799
BssMI GATC 2 cut(s) 390, 766
Bst2UI CCWGG 3 cut(s) 588, 709, 801
Bst4CI ACNGT 6 cut(s) 49, 145, 267, 655, 664, 1003
BstAPI GCANNNNNTGC 1 cut(s) 294
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 4 cut(s) 13, 19, 681, 993
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 2 cut(s) 359, 934
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 2 cut(s) 393, 769
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 2 cut(s) 390, 766
BstMWI GCNNNNNNNGC 4 cut(s) 23, 294, 450, 962
BstNI CCWGG 3 cut(s) 588, 709, 801
BstNSI RCATGY 3 cut(s) 215, 582, 731
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 4 cut(s) 156, 586, 707, 799
BstSFI CTRYAG 1 cut(s) 1022
BstV1I GCAGC 3 cut(s) 386, 903, 968
BstXI CCANNNNNNTGG 1 cut(s) 983
Bsu36I CCTNAGG 1 cut(s) 19
BsuRI GGCC 2 cut(s) 116, 543
BtsCI GGATG 2 cut(s) 359, 934
BtsIMutI CAGTG 3 cut(s) 45, 258, 493
BveI ACCTGC 1 cut(s) 971
Cac8I GCNNGC 1 cut(s) 197
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 2 cut(s) 188, 674
Cfr13I GGNCC 2 cut(s) 114, 584
Csp6I GTAC 5 cut(s) 678, 690, 697, 838, 999
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 6 cut(s) 111, 212, 579, 593, 728, 977
CviQI GTAC 5 cut(s) 678, 690, 697, 838, 999
DdeI CTNAG 4 cut(s) 13, 19, 681, 993
DpnI GATC 2 cut(s) 392, 768
DpnII GATC 2 cut(s) 390, 766
DraIII CACNNNGTG 2 cut(s) 388, 409
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 2 cut(s) 18, 981
Eco81I CCTNAGG 1 cut(s) 19
Eco91I GGTNACC 1 cut(s) 223
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 586, 707, 799
FaeI CATG 6 cut(s) 114, 215, 582, 596, 731, 980
FaqI GGGAC 2 cut(s) 140, 284
FatI CATG 6 cut(s) 110, 211, 578, 592, 727, 976
FbaI TGATCA 1 cut(s) 390
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 4 cut(s) 400, 451, 892, 957
FokI GGATG 2 cut(s) 346, 941
Fsp4HI GCNGC 4 cut(s) 400, 451, 892, 957
FspBI CTAG 1 cut(s) 419
GlaI GCGC 1 cut(s) 25
GluI GCNGC 4 cut(s) 400, 451, 892, 957
GsuI CTGGAG 1 cut(s) 834
HaeIII GGCC 2 cut(s) 116, 543
HapII CCGG 5 cut(s) 158, 189, 234, 285, 675
HhaI GCGC 1 cut(s) 26
Hin1II CATG 6 cut(s) 114, 215, 582, 596, 731, 980
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 2 cut(s) 120, 963
HinfI GANTC 4 cut(s) 67, 86, 281, 809
HpaII CCGG 5 cut(s) 158, 189, 234, 285, 675
HphI GGTGA 2 cut(s) 187, 664
Hpy166II GTNNAC 2 cut(s) 10, 483
Hpy188I TCNGA 1 cut(s) 684
Hpy188III TCNNGA 4 cut(s) 71, 245, 806, 813
Hpy8I GTNNAC 2 cut(s) 10, 483
Hpy99I CGWCG 1 cut(s) 788
HpyCH4III ACNGT 6 cut(s) 49, 145, 267, 655, 664, 1003
HpyCH4IV ACGT 4 cut(s) 408, 471, 573, 648
HpyCH4V TGCA 6 cut(s) 110, 276, 578, 643, 891, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 294, 450, 962
HpyF3I CTNAG 4 cut(s) 13, 19, 681, 993
HpySE526I ACGT 4 cut(s) 408, 471, 573, 648
Hsp92II CATG 6 cut(s) 114, 215, 582, 596, 731, 980
HspAI GCGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 2 cut(s) 390, 766
LmnI GCTCC 4 cut(s) 62, 305, 622, 899
Lsp1109I GCAGC 3 cut(s) 386, 903, 968
MaeI CTAG 1 cut(s) 419
MaeII ACGT 4 cut(s) 408, 471, 573, 648
MaeIII GTNAC 7 cut(s) 37, 49, 223, 490, 649, 664, 908
MalI GATC 2 cut(s) 392, 768
MboI GATC 2 cut(s) 390, 766
MfeI CAATTG 1 cut(s) 537
MluCI AATT 8 cut(s) 328, 510, 537, 556, 597, 772, 822, 881
MlyI GAGTC 3 cut(s) 80, 275, 818
MnlI CCTC 7 cut(s) 113, 448, 510, 554, 622, 919, 988
MseI TTAA 2 cut(s) 657, 933
MslI CAYNNNNRTG 3 cut(s) 216, 344, 981
MspA1I CMGCKG 2 cut(s) 959, 992
MspI CCGG 5 cut(s) 158, 189, 234, 285, 675
MspR9I CCNGG 4 cut(s) 158, 588, 709, 801
MunI CAATTG 1 cut(s) 537
Mva1269I GAATGC 2 cut(s) 98, 465
MvaI CCWGG 3 cut(s) 588, 709, 801
MwoI GCNNNNNNNGC 4 cut(s) 23, 294, 450, 962
NciI CCSGG 1 cut(s) 158
NdeII GATC 2 cut(s) 390, 766
NlaIII CATG 6 cut(s) 114, 215, 582, 596, 731, 980
NlaIV GGNNCC 1 cut(s) 550
NmeAIII GCCGAG 1 cut(s) 525
NmuCI GTSAC 3 cut(s) 37, 49, 490
NspI RCATGY 3 cut(s) 215, 582, 731
NspV TTCGAA 1 cut(s) 332
PaqCI CACCTGC 1 cut(s) 971
PctI GAATGC 2 cut(s) 98, 465
PfeI GAWTC 1 cut(s) 67
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 4 cut(s) 401, 452, 893, 958
PleI GAGTC 3 cut(s) 80, 275, 817
PpsI GAGTC 3 cut(s) 80, 275, 817
Psp1406I AACGTT 1 cut(s) 573
Psp6I CCWGG 3 cut(s) 586, 707, 799
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 3 cut(s) 586, 707, 799
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 2 cut(s) 114, 584
PvuII CAGCTG 2 cut(s) 959, 992
RsaI GTAC 5 cut(s) 679, 691, 698, 839, 1000
RsaNI GTAC 5 cut(s) 678, 690, 697, 838, 999
RseI CAYNNNNRTG 3 cut(s) 216, 344, 981
SaqAI TTAA 2 cut(s) 657, 933
SatI GCNGC 4 cut(s) 400, 451, 892, 957
Sau3AI GATC 2 cut(s) 390, 766
Sau96I GGNCC 2 cut(s) 114, 584
ScaI AGTACT 1 cut(s) 698
SchI GAGTC 3 cut(s) 80, 275, 818
ScrFI CCNGG 4 cut(s) 158, 588, 709, 801
SfcI CTRYAG 1 cut(s) 1022
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 3 cut(s) 216, 344, 981
Sse9I AATT 8 cut(s) 328, 510, 537, 556, 597, 772, 822, 881
SsiI CCGC 1 cut(s) 451
SspMI CTAG 1 cut(s) 419
StyD4I CCNGG 4 cut(s) 156, 586, 707, 799
TaaI ACNGT 6 cut(s) 49, 145, 267, 655, 664, 1003
TaiI ACGT 4 cut(s) 411, 474, 576, 651
TaqI TCGA 2 cut(s) 332, 885
TaqII GACCGA 1 cut(s) 98
TasI AATT 8 cut(s) 328, 510, 537, 556, 597, 772, 822, 881
TatI WGTACW 1 cut(s) 696
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 2 cut(s) 657, 933
Tru9I TTAA 2 cut(s) 657, 933
TscAI CASTG 3 cut(s) 52, 265, 493
TseFI GTSAC 3 cut(s) 37, 49, 490
TseI GCWGC 3 cut(s) 399, 891, 956
Tsp45I GTSAC 3 cut(s) 37, 49, 490
TspDTI ATGAA 2 cut(s) 17, 362
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 3 cut(s) 52, 265, 493
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 1 cut(s) 881
XceI RCATGY 3 cut(s) 215, 582, 731
XmiI GTMKAC 1 cut(s) 482
XspI CTAG 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.