MD00G1087900.v1.1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000702
Physical Location & Seq
Forward (+)
17831826 .. 17834170
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1087900.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGGCAAACCCCAGAAGCCTCACATATCCAGCAGCAGCAGTTTTTGCGTTGTTGATGATAAGCATTACTAATGTGGATGCTGCAGCCGTCACTTTCAGCGTGAGCAGTTTAGGAGCCAAAGCAGATGGCACGACTGACTCCACCAAGGCCTTCCTCTCTGCGTGGTCCAATGCTTGTGCCTCTGTCAACCCCGCTGTCATCTATGTCCCCGCAGGGAGGTTCTTGCTTAGCAATGCCGTGTTCTCTGGGCCATGCAAGAACAACGCCATCACCTTCCGCATTGCCGGCACCCTCGTCGCCCCGTCTGATTACCAGGTCATTGGAAACGCCGGTAACTGGCTTCTCTTTCAGCATGTCAATGGGGTCACCATTTCCGGTGGAGTTCTCGACGGTCAGGGCACCGGATTGTGGGATTGCAAGTCCTCAGGCAAGAGTTGCCCCAGCGGAGCAACTACACTGGGCTTTTCCAACTCCAACAACATTGTGGTGAGTGGATTAGTATCACTAAACAGCCAAATGTTCCACATTGTCGTCAACGGCTGCCAAAATGTGCAAATGCAAGGTGTCAAGGTTAACGCGGCCGGCAACAGCCCCAACACCGATGGCATCCATGTCCAAATGTCATCGGGAGTCACCATTCTCAACTCCAAAATTTCAACCGGTGACGACTGTGTCTCAGTTGGCCCCGGCACTACCAATTTGTGGATTGAAAACGTCGCATGTGGACCCGGCCACGGAATCAGCATTGGGAGTTTAGGGAAGGACCAACAAGAAGCTGGTGTACAAAATGTTACAGTTAAAACAATTACATTCACTGGTACCGAAAATGGCGTCAGAATTAAGTCTTGGGGGAGACCTAGCACTGGATTTGCTAGGAGCATTCTTTTCCAACATATTGTGATGACCAACGTTCAAAATCCAATCGTTATTGATCAAAATTACTGCCCTAATGACAAAGGTTGCCCCGGCCAAGCTTCTGGAGTTAAGGTCAGTGATGTGACGTATCAAGACATTCATGGTACATCGGCGACGGAAGTGGCAGTGAAATTCGATTGTAGTTCCATGTATCCTTGCAACGGGATCAGACTGCAAGATGTGAAGCTCACTTACAATAACCAAGCAGCTGAAGCTTCATGCATCCATGCAGGCGGAACAACTGCCGGTACGGTTCAGCCGACAAGTTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

397

Amino Acids

40.99

Weight (kDa)

7.0

Isoelectric Point (pI)

28.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 32 - 284 1.6e-15 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 59 - 380 9.1e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 671
Acc65I GGTACC 1 cut(s) 818
AccB1I GGYRCC 3 cut(s) 287, 398, 818
AccB7I CCANNNNNTGG 1 cut(s) 702
AccII CGCG 1 cut(s) 578
AciI CCGC 6 cut(s) 192, 210, 277, 444, 578, 1149
AclI AACGTT 1 cut(s) 909
AclWI GGATC 1 cut(s) 1088
AcoI YGGCCR 3 cut(s) 579, 730, 967
AcsI RAATTY 2 cut(s) 651, 1046
AcuI CTGAAG 1 cut(s) 1146
AcyI GRCGYC 1 cut(s) 831
AfaI GTAC 4 cut(s) 783, 820, 1021, 1165
AfiI CCNNNNNNNGG 7 cut(s) 216, 336, 408, 702, 734, 863, 1076
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 3 cut(s) 657, 710, 914
AjnI CCWGG 1 cut(s) 312
AjuI GAANNNNNNNTTGG 2 cut(s) 829, 861
AluBI AGCT 5 cut(s) 776, 974, 1102, 1124, 1130
AluI AGCT 5 cut(s) 776, 974, 1102, 1124, 1130
Alw26I GTCTC 2 cut(s) 679, 847
AlwI GGATC 1 cut(s) 1088
AoxI GGCC 6 cut(s) 147, 248, 579, 682, 730, 967
ApeKI GCWGC 6 cut(s) 32, 35, 80, 83, 540, 1121
ApoI RAATTY 2 cut(s) 651, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 816, 848
AsiGI ACCGGT 1 cut(s) 659
Asp718I GGTACC 1 cut(s) 818
AspS9I GGNCC 5 cut(s) 165, 248, 683, 725, 763
AsuC2I CCSGG 3 cut(s) 687, 729, 966
AsuHPI GGTGA 5 cut(s) 262, 358, 499, 625, 674
AvaII GGWCC 3 cut(s) 165, 725, 763
AxyI CCTNAGG 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 401
BanI GGYRCC 3 cut(s) 287, 398, 818
BarI GAAGNNNNNNTAC 4 cut(s) 765, 797, 1091, 1123
BbvI GCAGC 6 cut(s) 44, 47, 67, 95, 527, 1133
BccI CCATC 3 cut(s) 119, 275, 596
BceAI ACGGC 3 cut(s) 71, 221, 553
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 1 cut(s) 314
BciVI GTATCC 1 cut(s) 1077
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 3 cut(s) 687, 729, 966
BcoDI GTCTC 2 cut(s) 679, 847
BfaI CTAG 2 cut(s) 858, 873
BfmI CTRYAG 1 cut(s) 81
BfuI GTATCC 1 cut(s) 1077
BisI GCNGC 7 cut(s) 33, 36, 81, 84, 541, 579, 1122
BlpI GCTNAGC 1 cut(s) 227
BlsI GCNGC 7 cut(s) 34, 37, 82, 85, 542, 580, 1123
Bme1390I CCNGG 4 cut(s) 314, 687, 729, 966
Bme18I GGWCC 3 cut(s) 165, 725, 763
BmgT120I GGNCC 5 cut(s) 165, 248, 683, 725, 763
BmiI GGNNCC 6 cut(s) 115, 289, 400, 685, 727, 820
BmrFI CCNGG 4 cut(s) 314, 687, 729, 966
BmrI ACTGGG 1 cut(s) 467
BmsI GCATC 3 cut(s) 67, 615, 1146
BmuI ACTGGG 1 cut(s) 467
BpmI CTGGAG 1 cut(s) 999
Bpu1102I GCTNAGC 1 cut(s) 227
BpuMI CCSGG 3 cut(s) 687, 729, 966
BsaBI GATNNNNATC 1 cut(s) 499
BsaHI GRCGYC 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 847
BsaJI CCNNGG 4 cut(s) 144, 685, 733, 964
BsaWI WCCGGW 3 cut(s) 374, 401, 659
BsaXI ACNNNNNCTCC 2 cut(s) 972, 1002
Bsc4I CCNNNNNNNGG 7 cut(s) 216, 336, 408, 702, 734, 863, 1076
Bse118I RCCGGY 5 cut(s) 284, 329, 581, 659, 1160
Bse1I ACTGG 4 cut(s) 341, 462, 820, 868
Bse21I CCTNAGG 1 cut(s) 424
Bse3DI GCAATG 2 cut(s) 238, 279
Bse8I GATNNNNATC 1 cut(s) 499
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 4 cut(s) 144, 685, 733, 964
BseGI GGATG 3 cut(s) 82, 606, 1137
BseJI GATNNNNATC 1 cut(s) 499
BseLI CCNNNNNNNGG 7 cut(s) 216, 336, 408, 702, 734, 863, 1076
BseMI GCAATG 2 cut(s) 238, 279
BseMII CTCAG 2 cut(s) 438, 690
BseNI ACTGG 4 cut(s) 341, 462, 820, 868
BseSI GKGCMC 1 cut(s) 401
BseX3I CGGCCG 1 cut(s) 579
BseXI GCAGC 6 cut(s) 44, 47, 67, 95, 527, 1133
BseYI CCCAGC 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 578
Bsh1285I CGRYCG 1 cut(s) 582
BshFI GGCC 6 cut(s) 149, 250, 581, 684, 732, 969
BshNI GGYRCC 3 cut(s) 287, 398, 818
BshTI ACCGGT 1 cut(s) 659
BsiEI CGRYCG 1 cut(s) 582
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 7 cut(s) 216, 336, 408, 702, 734, 863, 1076
BsmAI GTCTC 2 cut(s) 679, 847
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 6 cut(s) 149, 250, 581, 684, 732, 969
Bso31I GGTCTC 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 401
Bsp1407I TGTACA 1 cut(s) 781
Bsp143I GATC 2 cut(s) 931, 1080
Bsp1720I GCTNAGC 1 cut(s) 227
BspACI CCGC 6 cut(s) 192, 210, 277, 444, 578, 1149
BspANI GGCC 6 cut(s) 149, 250, 581, 684, 732, 969
BspCNI CTCAG 2 cut(s) 437, 689
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 6 cut(s) 115, 289, 400, 685, 727, 820
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 1 cut(s) 1088
BspT107I GGYRCC 3 cut(s) 287, 398, 818
BspTNI GGTCTC 1 cut(s) 847
BsrDI GCAATG 2 cut(s) 238, 279
BsrFI RCCGGY 5 cut(s) 284, 329, 581, 659, 1160
BsrGI TGTACA 1 cut(s) 781
BsrI ACTGG 4 cut(s) 341, 462, 820, 868
BssAI RCCGGY 5 cut(s) 284, 329, 581, 659, 1160
BssECI CCNNGG 4 cut(s) 144, 685, 733, 964
BssMI GATC 2 cut(s) 931, 1080
BssNI GRCGYC 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 4 cut(s) 392, 671, 796, 1168
BstACI GRCGYC 1 cut(s) 831
BstAPI GCANNNNNTGC 2 cut(s) 44, 435
BstAUI TGTACA 1 cut(s) 781
BstC8I GCNNGC 3 cut(s) 286, 583, 1147
BstDEI CTNAG 3 cut(s) 227, 424, 676
BstDSI CCRYGG 1 cut(s) 733
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 3 cut(s) 82, 606, 1137
BstFNI CGCG 1 cut(s) 578
BstKTI GATC 2 cut(s) 934, 1083
BstMAI GTCTC 2 cut(s) 679, 847
BstMBI GATC 2 cut(s) 931, 1080
BstMCI CGRYCG 1 cut(s) 582
BstMWI GCNNNNNNNGC 4 cut(s) 44, 285, 435, 1127
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 2 cut(s) 356, 723
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 4 cut(s) 312, 685, 727, 964
BstSFI CTRYAG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 401
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 6 cut(s) 44, 47, 67, 95, 527, 1133
BstXI CCANNNNNNTGG 2 cut(s) 320, 977
BstZI CGGCCG 1 cut(s) 579
Bsu36I CCTNAGG 1 cut(s) 424
BsuI GTATCC 1 cut(s) 1077
BsuRI GGCC 6 cut(s) 149, 250, 581, 684, 732, 969
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 3 cut(s) 82, 606, 1137
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 5 cut(s) 455, 813, 861, 997, 1047
Cac8I GCNNGC 3 cut(s) 286, 583, 1147
Cfr10I RCCGGY 5 cut(s) 284, 329, 581, 659, 1160
Cfr13I GGNCC 5 cut(s) 165, 248, 683, 725, 763
CseI GACGC 1 cut(s) 820
CsiI ACCWGGT 1 cut(s) 312
Csp6I GTAC 4 cut(s) 782, 819, 1020, 1164
CspAI ACCGGT 1 cut(s) 659
CviAII CATG 8 cut(s) 252, 353, 611, 720, 1016, 1063, 1134, 1142
CviQI GTAC 4 cut(s) 782, 819, 1020, 1164
DdeI CTNAG 3 cut(s) 227, 424, 676
DpnI GATC 2 cut(s) 933, 1082
DpnII GATC 2 cut(s) 931, 1080
DrdI GACNNNNNNGTC 1 cut(s) 671
DseDI GACNNNNNNGTC 1 cut(s) 671
EaeI YGGCCR 3 cut(s) 579, 730, 967
EagI CGGCCG 1 cut(s) 579
EciI GGCGGA 1 cut(s) 1164
EclXI CGGCCG 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 144
Eco147I AGGCCT 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 847
Eco47I GGWCC 3 cut(s) 165, 725, 763
Eco52I CGGCCG 1 cut(s) 579
Eco57I CTGAAG 1 cut(s) 1146
Eco81I CCTNAGG 1 cut(s) 424
Eco91I GGTNACC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 1139
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 8 cut(s) 255, 356, 614, 723, 1019, 1066, 1137, 1145
FaqI GGGAC 1 cut(s) 191
FatI CATG 8 cut(s) 251, 352, 610, 719, 1015, 1062, 1133, 1141
FauI CCCGC 2 cut(s) 199, 217
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 7 cut(s) 33, 36, 81, 84, 541, 579, 1122
FokI GGATG 3 cut(s) 89, 593, 1124
Fsp4HI GCNGC 7 cut(s) 33, 36, 81, 84, 541, 579, 1122
FspBI CTAG 2 cut(s) 858, 873
GluI GCNGC 7 cut(s) 33, 36, 81, 84, 541, 579, 1122
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 1 cut(s) 999
HaeIII GGCC 6 cut(s) 149, 250, 581, 684, 732, 969
HgaI GACGC 1 cut(s) 820
Hin1I GRCGYC 1 cut(s) 831
Hin1II CATG 8 cut(s) 255, 356, 614, 723, 1019, 1066, 1137, 1145
HincII GTYRAC 3 cut(s) 187, 535, 574
HindII GTYRAC 3 cut(s) 187, 535, 574
HindIII AAGCTT 2 cut(s) 972, 1128
HinfI GANTC 3 cut(s) 137, 630, 738
HpaI GTTAAC 1 cut(s) 574
HphI GGTGA 5 cut(s) 262, 358, 499, 625, 674
Hpy166II GTNNAC 5 cut(s) 187, 535, 574, 725, 782
Hpy188I TCNGA 3 cut(s) 307, 836, 1085
Hpy188III TCNNGA 4 cut(s) 386, 627, 978, 1007
Hpy8I GTNNAC 5 cut(s) 187, 535, 574, 725, 782
Hpy99I CGWCG 4 cut(s) 299, 392, 719, 1033
HpyAV CCTTC 3 cut(s) 160, 283, 754
HpyCH4III ACNGT 4 cut(s) 392, 671, 796, 1168
HpyCH4IV ACGT 3 cut(s) 714, 909, 1001
HpyCH4V TGCA 9 cut(s) 83, 255, 417, 553, 559, 1074, 1090, 1137, 1145
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 285, 435, 1127
HpyF3I CTNAG 3 cut(s) 227, 424, 676
HpySE526I ACGT 3 cut(s) 714, 909, 1001
Hsp92I GRCGYC 1 cut(s) 831
Hsp92II CATG 8 cut(s) 255, 356, 614, 723, 1019, 1066, 1137, 1145
KpnI GGTACC 1 cut(s) 822
KroI GCCGGC 2 cut(s) 284, 581
KroNI GCCGGC 2 cut(s) 286, 583
Ksp22I TGATCA 1 cut(s) 931
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 2 cut(s) 931, 1080
LmnI GCTCC 3 cut(s) 113, 446, 876
Lsp1109I GCAGC 6 cut(s) 44, 47, 67, 95, 527, 1133
LweI GCATC 3 cut(s) 67, 615, 1146
MabI ACCWGGT 1 cut(s) 312
MaeI CTAG 2 cut(s) 858, 873
MaeII ACGT 3 cut(s) 714, 909, 1001
MaeIII GTNAC 7 cut(s) 88, 332, 364, 631, 662, 790, 997
MalI GATC 2 cut(s) 933, 1082
MboI GATC 2 cut(s) 931, 1080
MhlI GDGCHC 1 cut(s) 401
MluCI AATT 6 cut(s) 651, 697, 804, 837, 937, 1046
MlyI GAGTC 2 cut(s) 131, 639
MmeI TCCRAC 3 cut(s) 492, 498, 913
MnlI CCTC 6 cut(s) 29, 164, 190, 210, 302, 433
Mph1103I ATGCAT 1 cut(s) 1139
MroNI GCCGGC 2 cut(s) 284, 581
MseI TTAA 4 cut(s) 573, 798, 840, 984
MslI CAYNNNNRTG 2 cut(s) 357, 485
MspA1I CMGCKG 3 cut(s) 194, 444, 1124
MspR9I CCNGG 4 cut(s) 314, 687, 729, 966
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 4 cut(s) 44, 285, 435, 1127
NaeI GCCGGC 2 cut(s) 286, 583
NciI CCSGG 3 cut(s) 687, 729, 966
NdeII GATC 2 cut(s) 931, 1080
NgoMIV GCCGGC 2 cut(s) 284, 581
NlaIII CATG 8 cut(s) 255, 356, 614, 723, 1019, 1066, 1137, 1145
NlaIV GGNNCC 6 cut(s) 115, 289, 400, 685, 727, 820
NmuCI GTSAC 5 cut(s) 88, 364, 631, 662, 997
NsiI ATGCAT 1 cut(s) 1139
NspI RCATGY 2 cut(s) 356, 723
PceI AGGCCT 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 1172
PctI GAATGC 1 cut(s) 879
PdiI GCCGGC 2 cut(s) 286, 583
PfeI GAWTC 1 cut(s) 738
PflFI GACNNNGTC 1 cut(s) 671
PflMI CCANNNNNTGG 1 cut(s) 702
PinAI ACCGGT 1 cut(s) 659
PkrI GCNGC 7 cut(s) 34, 37, 82, 85, 542, 580, 1123
PleI GAGTC 2 cut(s) 131, 638
PpsI GAGTC 2 cut(s) 131, 638
Psp1406I AACGTT 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 312
PspEI GGTNACC 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 6 cut(s) 115, 289, 400, 685, 727, 820
PspPI GGNCC 5 cut(s) 165, 248, 683, 725, 763
PstI CTGCAG 1 cut(s) 85
PsyI GACNNNGTC 1 cut(s) 671
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 4 cut(s) 783, 820, 1021, 1165
RsaNI GTAC 4 cut(s) 782, 819, 1020, 1164
RseI CAYNNNNRTG 2 cut(s) 357, 485
SaqAI TTAA 4 cut(s) 573, 798, 840, 984
SatI GCNGC 7 cut(s) 33, 36, 81, 84, 541, 579, 1122
Sau3AI GATC 2 cut(s) 931, 1080
Sau96I GGNCC 5 cut(s) 165, 248, 683, 725, 763
SchI GAGTC 2 cut(s) 131, 639
ScrFI CCNGG 4 cut(s) 314, 687, 729, 966
SduI GDGCHC 1 cut(s) 401
SexAI ACCWGGT 1 cut(s) 312
SfaNI GCATC 3 cut(s) 67, 615, 1146
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 3 cut(s) 165, 725, 763
SmiMI CAYNNNNRTG 2 cut(s) 357, 485
Sse9I AATT 6 cut(s) 651, 697, 804, 837, 937, 1046
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 6 cut(s) 192, 210, 277, 444, 578, 1149
SspMI CTAG 2 cut(s) 858, 873
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 4 cut(s) 312, 685, 727, 964
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 392, 671, 796, 1168
TaiI ACGT 3 cut(s) 717, 912, 1004
TaqI TCGA 2 cut(s) 387, 1050
TasI AATT 6 cut(s) 651, 697, 804, 837, 937, 1046
TatI WGTACW 1 cut(s) 781
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 1 cut(s) 738
Tru1I TTAA 4 cut(s) 573, 798, 840, 984
Tru9I TTAA 4 cut(s) 573, 798, 840, 984
TscAI CASTG 5 cut(s) 462, 820, 868, 997, 1047
TseFI GTSAC 5 cut(s) 88, 364, 631, 662, 997
TseI GCWGC 6 cut(s) 32, 35, 80, 83, 540, 1121
Tsp45I GTSAC 5 cut(s) 88, 364, 631, 662, 997
TspDTI ATGAA 2 cut(s) 1004, 1122
TspGWI ACGGA 2 cut(s) 750, 1046
TspRI CASTG 5 cut(s) 462, 820, 868, 997, 1047
Tth111I GACNNNGTC 1 cut(s) 671
Van91I CCANNNNNTGG 1 cut(s) 702
VpaK11BI GGWCC 3 cut(s) 165, 725, 763
XapI RAATTY 2 cut(s) 651, 1046
XceI RCATGY 2 cut(s) 356, 723
XcmI CCANNNNNNNNNTGG 2 cut(s) 481, 773
XspI CTAG 2 cut(s) 858, 873
Zsp2I ATGCAT 1 cut(s) 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.