pycom02g00030

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000702
Physical Location & Seq
Forward (+)
28051 .. 30197
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00030.3

Sequence Viewer

Length: 1197 bp
ATGGCAAACCCCAAAAGCCTCTCATATCCAGCAGCTGCAGTTTTTGCGTTGTTGATGATGGCTATAAGCATTACTAATGTGGATGCTGCAGCCGTCACTTTCAGTGTGAGCAGTTTAGGAGCCAAAGCAGATGGCAGTACTGACTCCACCAAGGCCTTCCTCTCTGCGTGGTCCAATGCTTGTGCCTCCGTCAACCCCGCTGTCATCTATGTCCCCGCAGGGAGGTTCTTGCTTGGCAATGCCGTGTTCTCTGGCCCATGCAAGAACAACGCCATCACCTTCCGCATTGCTGGCACCCTTGTCGCCCCGTCTGATTACCAGGTCATTGGAAATGCCGGTAACTGGCTTCTCTTCCAGCATGTCAATGGGGTCACCATTTCCGGTGGAGTTCTTGATGGTCAGGGCACCAGATTGTGGGATTGCAAGTCCTCAGGCAAGAGTTGCCCCAGCGGAGCAACTACACTGGGCTTTTCCAACTCCAACAACGTTGTGGTGAGTGGATTAGTATCACTAAACAGCCAAATGTTCCACATTGTCGTCAACGGCTGCCAAAATGTGAAAATGCAAGGTGTCAAGGTTAACGCGGCCGGCAACAGCCCCAACACCGATGGCATCCATGTCCAAATGTCATCTGGAGTCACCATTCTCGACTCCAAAATTTCAACCGGTGACGACTGTGTCTCAGTTGGCCCCGGCACTACCAATTTGTGGATTGAAAACGTCGCATGTGGACCCGGCCACGGAATCAGCATTGGGAGTTTAGGGAAGGAGCAACAAGAAGCCGGTGTACAAAATGTTACAGTTAAAACAATTACATTCACTGGTACTGAAAACGGCGTCAGAATTAAGTCTTGGGGAAGACCTAGCACTGGATTTGCTAGGAGCATTCTTTTCCAACATATTGTGATGACCAATGTTCAAAATCCAATCGTTATTGATCAAAATTACTGCCCTAATGACAAAGGTTGCCCCGGCCAAGCTTCTGGAGTTAAGGTCAGCGATGTGACGTATGAAGATATTCATGGTACATCCGCGACGGAAGTGGCGGTGAAATTCGATTGTAGTTCCATGTATCCTTGCAACGGGATCAGACTGCAAGATGTGAAGCTCACTTACAATAACCAAGCAGCTGAAGCTTCATGCATCCATGCAGGCGGAACAACTGCCGGTACGGTTCAGCCGACAAGTTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

399

Amino Acids

41.21

Weight (kDa)

7.0

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 677
AccB1I GGYRCC 2 cut(s) 293, 404
AccB7I CCANNNNNTGG 2 cut(s) 414, 708
AccII CGCG 2 cut(s) 584, 1034
AciI CCGC 8 cut(s) 198, 216, 283, 450, 584, 1032, 1046, 1155
AclI AACGTT 1 cut(s) 486
AclWI GGATC 1 cut(s) 1094
AcoI YGGCCR 3 cut(s) 585, 736, 973
AcsI RAATTY 2 cut(s) 657, 1052
AcuI CTGAAG 1 cut(s) 1152
AcyI GRCGYC 1 cut(s) 837
AfaI GTAC 5 cut(s) 139, 789, 826, 1027, 1171
AfiI CCNNNNNNNGG 7 cut(s) 222, 342, 414, 708, 740, 869, 1082
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 3 cut(s) 663, 716, 920
AjnI CCWGG 1 cut(s) 318
AjuI GAANNNNNNNTTGG 2 cut(s) 835, 867
AluBI AGCT 5 cut(s) 35, 980, 1108, 1130, 1136
AluI AGCT 5 cut(s) 35, 980, 1108, 1130, 1136
Alw26I GTCTC 1 cut(s) 685
AlwI GGATC 1 cut(s) 1094
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 6 cut(s) 153, 253, 585, 688, 736, 973
ApeKI GCWGC 6 cut(s) 32, 35, 86, 89, 546, 1127
ApoI RAATTY 2 cut(s) 657, 1052
AsiGI ACCGGT 1 cut(s) 665
Asp700I GAANNNNTTC 1 cut(s) 1017
AspS9I GGNCC 4 cut(s) 171, 254, 689, 731
AsuC2I CCSGG 3 cut(s) 693, 735, 972
AsuHPI GGTGA 6 cut(s) 268, 364, 505, 631, 680, 1060
AvaII GGWCC 2 cut(s) 171, 731
AxyI CCTNAGG 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 407
BanI GGYRCC 2 cut(s) 293, 404
BarI GAAGNNNNNNTAC 4 cut(s) 771, 803, 1097, 1129
BbsI GAAGAC 1 cut(s) 865
BbvI GCAGC 6 cut(s) 22, 44, 73, 101, 533, 1139
BccI CCATC 5 cut(s) 52, 125, 281, 389, 602
BceAI ACGGC 4 cut(s) 77, 227, 559, 850
BcgI CGANNNNNNTGC 2 cut(s) 283, 317
BciT130I CCWGG 1 cut(s) 320
BciVI GTATCC 1 cut(s) 1083
BclI TGATCA 1 cut(s) 937
BcnI CCSGG 3 cut(s) 693, 735, 972
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 2 cut(s) 864, 879
BfmI CTRYAG 2 cut(s) 36, 87
BfuI GTATCC 1 cut(s) 1083
BisI GCNGC 7 cut(s) 33, 36, 87, 90, 547, 585, 1128
BlsI GCNGC 7 cut(s) 34, 37, 88, 91, 548, 586, 1129
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 4 cut(s) 320, 693, 735, 972
Bme18I GGWCC 2 cut(s) 171, 731
BmgT120I GGNCC 4 cut(s) 171, 254, 689, 731
BmiI GGNNCC 5 cut(s) 121, 295, 406, 691, 733
BmrFI CCNGG 4 cut(s) 320, 693, 735, 972
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 3 cut(s) 73, 621, 1152
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 1 cut(s) 865
BpmI CTGGAG 2 cut(s) 654, 1005
BpuMI CCSGG 3 cut(s) 693, 735, 972
BsaBI GATNNNNATC 1 cut(s) 505
BsaHI GRCGYC 1 cut(s) 837
BsaJI CCNNGG 4 cut(s) 150, 691, 739, 970
BsaWI WCCGGW 2 cut(s) 380, 665
BsaXI ACNNNNNCTCC 2 cut(s) 978, 1008
Bsc4I CCNNNNNNNGG 7 cut(s) 222, 342, 414, 708, 740, 869, 1082
Bse118I RCCGGY 5 cut(s) 335, 587, 665, 782, 1166
Bse1I ACTGG 4 cut(s) 347, 468, 826, 874
Bse21I CCTNAGG 1 cut(s) 430
Bse3DI GCAATG 2 cut(s) 244, 285
Bse8I GATNNNNATC 1 cut(s) 505
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 4 cut(s) 150, 691, 739, 970
BseGI GGATG 4 cut(s) 88, 612, 1028, 1143
BseJI GATNNNNATC 1 cut(s) 505
BseLI CCNNNNNNNGG 7 cut(s) 222, 342, 414, 708, 740, 869, 1082
BseMI GCAATG 2 cut(s) 244, 285
BseMII CTCAG 2 cut(s) 444, 696
BseNI ACTGG 4 cut(s) 347, 468, 826, 874
BseSI GKGCMC 1 cut(s) 407
BseX3I CGGCCG 1 cut(s) 585
BseXI GCAGC 6 cut(s) 22, 44, 73, 101, 533, 1139
BseYI CCCAGC 1 cut(s) 446
Bsh1236I CGCG 2 cut(s) 584, 1034
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 6 cut(s) 155, 255, 587, 690, 738, 975
BshNI GGYRCC 2 cut(s) 293, 404
BshTI ACCGGT 1 cut(s) 665
BsiEI CGRYCG 1 cut(s) 588
BsiSI CCGG 9 cut(s) 336, 381, 588, 666, 693, 735, 783, 972, 1167
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 7 cut(s) 222, 342, 414, 708, 740, 869, 1082
BsmAI GTCTC 1 cut(s) 685
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 885
BsnI GGCC 6 cut(s) 155, 255, 587, 690, 738, 975
Bsp1286I GDGCHC 1 cut(s) 407
Bsp1407I TGTACA 1 cut(s) 787
Bsp143I GATC 2 cut(s) 937, 1086
BspACI CCGC 8 cut(s) 198, 216, 283, 450, 584, 1032, 1046, 1155
BspANI GGCC 6 cut(s) 155, 255, 587, 690, 738, 975
BspCNI CTCAG 2 cut(s) 443, 695
BspFNI CGCG 2 cut(s) 584, 1034
BspLI GGNNCC 5 cut(s) 121, 295, 406, 691, 733
BspMAI CTGCAG 2 cut(s) 40, 91
BspPI GGATC 1 cut(s) 1094
BspT107I GGYRCC 2 cut(s) 293, 404
BsrDI GCAATG 2 cut(s) 244, 285
BsrFI RCCGGY 5 cut(s) 335, 587, 665, 782, 1166
BsrGI TGTACA 1 cut(s) 787
BsrI ACTGG 4 cut(s) 347, 468, 826, 874
BssAI RCCGGY 5 cut(s) 335, 587, 665, 782, 1166
BssECI CCNNGG 4 cut(s) 150, 691, 739, 970
BssMI GATC 2 cut(s) 937, 1086
BssNI GRCGYC 1 cut(s) 837
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 3 cut(s) 677, 802, 1174
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 1 cut(s) 837
BstAPI GCANNNNNTGC 2 cut(s) 44, 441
BstAUI TGTACA 1 cut(s) 787
BstC8I GCNNGC 3 cut(s) 292, 589, 1153
BstDEI CTNAG 2 cut(s) 430, 682
BstDSI CCRYGG 1 cut(s) 739
BstEII GGTNACC 1 cut(s) 370
BstF5I GGATG 4 cut(s) 88, 612, 1028, 1143
BstFNI CGCG 2 cut(s) 584, 1034
BstKTI GATC 2 cut(s) 940, 1089
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 2 cut(s) 937, 1086
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 4 cut(s) 44, 291, 441, 1133
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 2 cut(s) 362, 729
BstPI GGTNACC 1 cut(s) 370
BstSCI CCNGG 4 cut(s) 318, 691, 733, 970
BstSFI CTRYAG 2 cut(s) 36, 87
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 2 cut(s) 584, 1034
BstV1I GCAGC 6 cut(s) 22, 44, 73, 101, 533, 1139
BstV2I GAAGAC 1 cut(s) 865
BstXI CCANNNNNNTGG 2 cut(s) 326, 983
BstZI CGGCCG 1 cut(s) 585
Bsu36I CCTNAGG 1 cut(s) 430
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 6 cut(s) 155, 255, 587, 690, 738, 975
BtgI CCRYGG 1 cut(s) 739
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 4 cut(s) 88, 612, 1028, 1143
BtsIMutI CAGTG 4 cut(s) 109, 461, 819, 867
Cac8I GCNNGC 3 cut(s) 292, 589, 1153
CaiI CAGNNNCTG 1 cut(s) 35
Cfr10I RCCGGY 5 cut(s) 335, 587, 665, 782, 1166
Cfr13I GGNCC 4 cut(s) 171, 254, 689, 731
CseI GACGC 1 cut(s) 826
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 5 cut(s) 138, 788, 825, 1026, 1170
CspAI ACCGGT 1 cut(s) 665
CviAII CATG 8 cut(s) 258, 359, 617, 726, 1022, 1069, 1140, 1148
CviQI GTAC 5 cut(s) 138, 788, 825, 1026, 1170
DdeI CTNAG 2 cut(s) 430, 682
DpnI GATC 2 cut(s) 939, 1088
DpnII GATC 2 cut(s) 937, 1086
DrdI GACNNNNNNGTC 1 cut(s) 677
DseDI GACNNNNNNGTC 1 cut(s) 677
EaeI YGGCCR 3 cut(s) 585, 736, 973
EagI CGGCCG 1 cut(s) 585
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 1 cut(s) 1170
EclXI CGGCCG 1 cut(s) 585
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 171, 731
Eco52I CGGCCG 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 1152
Eco81I CCTNAGG 1 cut(s) 430
Eco91I GGTNACC 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 1145
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 8 cut(s) 261, 362, 620, 729, 1025, 1072, 1143, 1151
FaqI GGGAC 1 cut(s) 197
FatI CATG 8 cut(s) 257, 358, 616, 725, 1021, 1068, 1139, 1147
FauI CCCGC 2 cut(s) 205, 223
FbaI TGATCA 1 cut(s) 937
Fnu4HI GCNGC 7 cut(s) 33, 36, 87, 90, 547, 585, 1128
FokI GGATG 4 cut(s) 95, 599, 1015, 1130
Fsp4HI GCNGC 7 cut(s) 33, 36, 87, 90, 547, 585, 1128
FspBI CTAG 2 cut(s) 864, 879
GluI GCNGC 7 cut(s) 33, 36, 87, 90, 547, 585, 1128
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 2 cut(s) 654, 1005
HaeIII GGCC 6 cut(s) 155, 255, 587, 690, 738, 975
HapII CCGG 9 cut(s) 336, 381, 588, 666, 693, 735, 783, 972, 1167
HgaI GACGC 1 cut(s) 826
Hin1I GRCGYC 1 cut(s) 837
Hin1II CATG 8 cut(s) 261, 362, 620, 729, 1025, 1072, 1143, 1151
HincII GTYRAC 3 cut(s) 193, 541, 580
HindII GTYRAC 3 cut(s) 193, 541, 580
HindIII AAGCTT 2 cut(s) 978, 1134
HinfI GANTC 4 cut(s) 143, 636, 650, 744
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 9 cut(s) 336, 381, 588, 666, 693, 735, 783, 972, 1167
HphI GGTGA 6 cut(s) 268, 364, 505, 631, 680, 1060
Hpy166II GTNNAC 5 cut(s) 193, 541, 580, 731, 788
Hpy188I TCNGA 3 cut(s) 313, 842, 1091
Hpy188III TCNNGA 4 cut(s) 392, 633, 647, 984
Hpy8I GTNNAC 5 cut(s) 193, 541, 580, 731, 788
Hpy99I CGWCG 2 cut(s) 725, 1039
HpyAV CCTTC 3 cut(s) 166, 289, 760
HpyCH4III ACNGT 3 cut(s) 677, 802, 1174
HpyCH4IV ACGT 3 cut(s) 486, 720, 1007
HpyCH4V TGCA 9 cut(s) 38, 89, 261, 423, 565, 1080, 1096, 1143, 1151
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 291, 441, 1133
HpyF3I CTNAG 2 cut(s) 430, 682
HpySE526I ACGT 3 cut(s) 486, 720, 1007
Hsp92I GRCGYC 1 cut(s) 837
Hsp92II CATG 8 cut(s) 261, 362, 620, 729, 1025, 1072, 1143, 1151
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 937
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 2 cut(s) 937, 1086
LmnI GCTCC 4 cut(s) 119, 452, 769, 882
Lsp1109I GCAGC 6 cut(s) 22, 44, 73, 101, 533, 1139
LweI GCATC 3 cut(s) 73, 621, 1152
MabI ACCWGGT 1 cut(s) 318
MaeI CTAG 2 cut(s) 864, 879
MaeII ACGT 3 cut(s) 486, 720, 1007
MaeIII GTNAC 7 cut(s) 94, 338, 370, 637, 668, 796, 1003
MalI GATC 2 cut(s) 939, 1088
MboI GATC 2 cut(s) 937, 1086
MboII GAAGA 3 cut(s) 343, 870, 1025
MhlI GDGCHC 1 cut(s) 407
MluCI AATT 6 cut(s) 657, 703, 810, 843, 943, 1052
MlyI GAGTC 3 cut(s) 137, 644, 645
MmeI TCCRAC 3 cut(s) 498, 504, 919
MnlI CCTC 5 cut(s) 29, 170, 196, 216, 439
Mph1103I ATGCAT 1 cut(s) 1145
MroNI GCCGGC 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 1017
MseI TTAA 4 cut(s) 579, 804, 846, 990
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 4 cut(s) 35, 200, 450, 1130
MspI CCGG 9 cut(s) 336, 381, 588, 666, 693, 735, 783, 972, 1167
MspR9I CCNGG 4 cut(s) 320, 693, 735, 972
Mva1269I GAATGC 1 cut(s) 885
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 2 cut(s) 584, 1034
MwoI GCNNNNNNNGC 4 cut(s) 44, 291, 441, 1133
NaeI GCCGGC 1 cut(s) 589
NciI CCSGG 3 cut(s) 693, 735, 972
NdeII GATC 2 cut(s) 937, 1086
NgoMIV GCCGGC 1 cut(s) 587
NlaIII CATG 8 cut(s) 261, 362, 620, 729, 1025, 1072, 1143, 1151
NlaIV GGNNCC 5 cut(s) 121, 295, 406, 691, 733
NmuCI GTSAC 5 cut(s) 94, 370, 637, 668, 1003
NsiI ATGCAT 1 cut(s) 1145
NspI RCATGY 2 cut(s) 362, 729
PceI AGGCCT 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 1178
PctI GAATGC 1 cut(s) 885
PdiI GCCGGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 1 cut(s) 744
PflFI GACNNNGTC 1 cut(s) 677
PflMI CCANNNNNTGG 2 cut(s) 414, 708
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 7 cut(s) 34, 37, 88, 91, 548, 586, 1129
PleI GAGTC 3 cut(s) 137, 644, 644
PpsI GAGTC 3 cut(s) 137, 644, 644
Psp1406I AACGTT 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 318
PspEI GGTNACC 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 5 cut(s) 121, 295, 406, 691, 733
PspPI GGNCC 4 cut(s) 171, 254, 689, 731
PstI CTGCAG 2 cut(s) 40, 91
PstNI CAGNNNCTG 1 cut(s) 35
PsyI GACNNNGTC 1 cut(s) 677
PvuII CAGCTG 2 cut(s) 35, 1130
RsaI GTAC 5 cut(s) 139, 789, 826, 1027, 1171
RsaNI GTAC 5 cut(s) 138, 788, 825, 1026, 1170
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 4 cut(s) 579, 804, 846, 990
SatI GCNGC 7 cut(s) 33, 36, 87, 90, 547, 585, 1128
Sau3AI GATC 2 cut(s) 937, 1086
Sau96I GGNCC 4 cut(s) 171, 254, 689, 731
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 3 cut(s) 137, 644, 645
ScrFI CCNGG 4 cut(s) 320, 693, 735, 972
SduI GDGCHC 1 cut(s) 407
SexAI ACCWGGT 1 cut(s) 318
SfaNI GCATC 3 cut(s) 73, 621, 1152
SfcI CTRYAG 2 cut(s) 36, 87
SinI GGWCC 2 cut(s) 171, 731
SmiMI CAYNNNNRTG 1 cut(s) 363
Sse9I AATT 6 cut(s) 657, 703, 810, 843, 943, 1052
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 8 cut(s) 198, 216, 283, 450, 584, 1032, 1046, 1155
SspMI CTAG 2 cut(s) 864, 879
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 4 cut(s) 318, 691, 733, 970
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 677, 802, 1174
TaiI ACGT 3 cut(s) 489, 723, 1010
TaqI TCGA 2 cut(s) 648, 1056
TasI AATT 6 cut(s) 657, 703, 810, 843, 943, 1052
TatI WGTACW 2 cut(s) 137, 787
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 1 cut(s) 744
Tru1I TTAA 4 cut(s) 579, 804, 846, 990
Tru9I TTAA 4 cut(s) 579, 804, 846, 990
TscAI CASTG 4 cut(s) 109, 468, 826, 874
TseFI GTSAC 5 cut(s) 94, 370, 637, 668, 1003
TseI GCWGC 6 cut(s) 32, 35, 86, 89, 546, 1127
Tsp45I GTSAC 5 cut(s) 94, 370, 637, 668, 1003
TspDTI ATGAA 3 cut(s) 1010, 1026, 1128
TspGWI ACGGA 3 cut(s) 178, 756, 1052
TspRI CASTG 4 cut(s) 109, 468, 826, 874
Tth111I GACNNNGTC 1 cut(s) 677
Van91I CCANNNNNTGG 2 cut(s) 414, 708
VpaK11BI GGWCC 2 cut(s) 171, 731
XapI RAATTY 2 cut(s) 657, 1052
XceI RCATGY 2 cut(s) 362, 729
XcmI CCANNNNNNNNNTGG 3 cut(s) 362, 487, 629
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 2 cut(s) 864, 879
ZrmI AGTACT 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.