RLG00000018212

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000702
Physical Location & Seq
Reverse (-)
26918211 .. 26919829
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018212

Sequence Viewer

Length: 1065 bp
ATGGAAAATCCCATAAGCCTCATTTTTCTCTCACTTCTTATCGTCTTCATAAACTCAGCCGTATCAACTGCATTCACTTACAATGTGGCCGATTTAGGAGCCAAACCAGATGGCAAAACTGACTCAACCAAGGCCTTCCTTTCTGGATGGGCCGAAGCTTATGCCTCGGCCAAGCCTCTGTCATTTATGTCCCAGCCGGAAGATTACCAGGTCATAGGAAATGCTGGTAACTGGCTTTTCTTTAGGCATGTGAATGGGGTTACCATCTCCGGTGGAATTCTTGACGGACAAGGCACTGGTTTGTGGGATTGCAAGGCCTCCGGCAAGACACTGGAGTTTTCCAACTCGAACAACATTGTCGTGAGTGGAGTGACATCCCTAAACGCCCAAATGTTCCACATAGTCATCAACAGCTGCAACAATGTGAAAATGCAAGGTGTTAGGGTTTCTGCCTCTGGTCACAGCCCAAACATCGATGGAATCCACGTCCAAAAGTCCTCAGGCGTCACTATTCTCGACTCTAAGATTGCAATCGGTGACGACTGTGTCTCAATTGGTCCGGCAACAACTAATCTATGGATTGAAAACGTCGCATGTGGACCCGGCCATGGAATCAGCATCGGGAGTTTAGGCAAGGACCTGGAGGAGGATGGAGTGCAAAATGTAACGGTCAAAACTGTTACATTTACAAGTACTCAAAATGGTCTGAGAATCAAATCATGGGGAAGACCCAGTACTGGGTTTGCTAAGAACATTCTTTTCCAACATGCTGTCATGGTCAATGTCCAAAATCCAATCATTATTGATCAAAATTATTGCCCAGGCAAAAAAGGGTGCCCTGGTCAGGTTTCGGGAGTTCAAATTAGTGACGTGACATATCAAAACATCCACGGCACATCAGCAACACAAGTTGCTGTGAAATTCGACTGTAGTTCTAGGTACCCTTGTAGCAAGATCACACTGGATGATGTGAAGCTCAGTTACAAGAACCAAGCAGCCGAAGCTTCATGTAGCCATGCCAGTGGAACTGCTGAGGGTATGGTTCAGCCGACAAGTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

355

Amino Acids

37.43

Weight (kDa)

6.43

Isoelectric Point (pI)

29.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 76 - 340 1.3e-76 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 545
Acc65I GGTACC 1 cut(s) 939
AccB1I GGYRCC 2 cut(s) 834, 939
AcoI YGGCCR 3 cut(s) 87, 168, 604
AcsI RAATTY 2 cut(s) 276, 920
AcyI GRCGYC 1 cut(s) 504
AfaI GTAC 3 cut(s) 694, 736, 941
AfiI CCNNNNNNNGG 5 cut(s) 608, 646, 737, 738, 844
AgsI TTSAA 2 cut(s) 584, 860
AjiI CACGTC 2 cut(s) 487, 871
AjnI CCWGG 4 cut(s) 207, 639, 820, 838
AluBI AGCT 4 cut(s) 158, 414, 976, 1004
AluI AGCT 4 cut(s) 158, 414, 976, 1004
Alw26I GTCTC 1 cut(s) 553
AoxI GGCC 6 cut(s) 87, 132, 150, 168, 315, 604
ApeKI GCWGC 2 cut(s) 414, 995
ApoI RAATTY 2 cut(s) 276, 920
ArsI GACNNNNNNTTYG 1 cut(s) 1042
Asp718I GGTACC 1 cut(s) 939
AspS9I GGNCC 4 cut(s) 150, 557, 599, 637
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 1 cut(s) 548
AvaII GGWCC 3 cut(s) 557, 599, 637
AxyI CCTNAGG 1 cut(s) 499
BaeGI GKGCMC 1 cut(s) 839
BaeI ACNNNNGTAYC 2 cut(s) 931, 964
BanI GGYRCC 2 cut(s) 834, 939
BarI GAAGNNNNNNTAC 4 cut(s) 718, 750, 965, 997
BbsI GAAGAC 2 cut(s) 37, 733
BbvCI CCTCAGC 1 cut(s) 1032
BbvI GCAGC 2 cut(s) 401, 1007
BccI CCATC 5 cut(s) 104, 141, 272, 470, 644
BceAI ACGGC 2 cut(s) 44, 907
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 4 cut(s) 209, 641, 822, 840
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 553
BfaI CTAG 1 cut(s) 936
BfmI CTRYAG 1 cut(s) 928
BisI GCNGC 2 cut(s) 415, 996
BlsI GCNGC 2 cut(s) 416, 997
BmcAI AGTACT 2 cut(s) 694, 736
Bme1390I CCNGG 5 cut(s) 209, 603, 641, 822, 840
Bme18I GGWCC 3 cut(s) 557, 599, 637
BmgBI CACGTC 2 cut(s) 487, 871
BmgT120I GGNCC 4 cut(s) 150, 557, 599, 637
BmiI GGNNCC 4 cut(s) 100, 601, 836, 941
BmrFI CCNGG 5 cut(s) 209, 603, 641, 822, 840
BmrI ACTGGG 2 cut(s) 726, 747
BmsI GCATC 1 cut(s) 627
BmuI ACTGGG 2 cut(s) 726, 747
BpiI GAAGAC 2 cut(s) 37, 733
BpmI CTGGAG 2 cut(s) 353, 662
Bpu10I CCTNAGC 1 cut(s) 1032
BpuMI CCSGG 1 cut(s) 603
Bsa29I ATCGAT 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 504
BsaJI CCNNGG 6 cut(s) 129, 165, 607, 820, 838, 889
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 5 cut(s) 608, 646, 737, 738, 844
Bse1I ACTGG 7 cut(s) 236, 301, 336, 732, 742, 966, 1020
Bse21I CCTNAGG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 4 cut(s) 209, 641, 822, 840
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 6 cut(s) 129, 165, 607, 820, 838, 889
BseGI GGATG 5 cut(s) 152, 374, 655, 885, 970
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 5 cut(s) 608, 646, 737, 738, 844
BseMII CTCAG 5 cut(s) 69, 513, 698, 991, 1023
BseNI ACTGG 7 cut(s) 236, 301, 336, 732, 742, 966, 1020
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 839
BseXI GCAGC 2 cut(s) 401, 1007
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 6 cut(s) 89, 134, 152, 170, 317, 606
BshNI GGYRCC 2 cut(s) 834, 939
BshVI ATCGAT 1 cut(s) 474
BsiSI CCGG 5 cut(s) 197, 270, 321, 560, 603
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 5 cut(s) 608, 646, 737, 738, 844
BsmAI GTCTC 1 cut(s) 553
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 6 cut(s) 89, 134, 152, 170, 317, 606
Bsp1286I GDGCHC 1 cut(s) 839
Bsp143I GATC 2 cut(s) 805, 954
Bsp19I CCATGG 1 cut(s) 607
BspANI GGCC 6 cut(s) 89, 134, 152, 170, 317, 606
BspCNI CTCAG 5 cut(s) 68, 512, 699, 990, 1024
BspDI ATCGAT 1 cut(s) 474
BspLI GGNNCC 4 cut(s) 100, 601, 836, 941
BspT107I GGYRCC 2 cut(s) 834, 939
BsrI ACTGG 7 cut(s) 236, 301, 336, 732, 742, 966, 1020
BssECI CCNNGG 6 cut(s) 129, 165, 607, 820, 838, 889
BssMI GATC 2 cut(s) 805, 954
BssNI GRCGYC 1 cut(s) 504
BssT1I CCWWGG 2 cut(s) 129, 607
Bst2UI CCWGG 4 cut(s) 209, 641, 822, 840
Bst4CI ACNGT 4 cut(s) 545, 670, 679, 929
BstACI GRCGYC 1 cut(s) 504
BstDEI CTNAG 7 cut(s) 55, 499, 522, 707, 747, 977, 1032
BstDSI CCRYGG 2 cut(s) 607, 889
BstEII GGTNACC 1 cut(s) 259
BstENI CCTNNNNNAGG 1 cut(s) 644
BstF5I GGATG 5 cut(s) 152, 374, 655, 885, 970
BstKTI GATC 2 cut(s) 808, 957
BstMAI GTCTC 1 cut(s) 553
BstMBI GATC 2 cut(s) 805, 954
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 4 cut(s) 209, 641, 822, 840
BstNSI RCATGY 3 cut(s) 251, 597, 770
BstPI GGTNACC 1 cut(s) 259
BstSCI CCNGG 5 cut(s) 207, 601, 639, 820, 838
BstSFI CTRYAG 1 cut(s) 928
BstSLI GKGCMC 1 cut(s) 839
BstV1I GCAGC 2 cut(s) 401, 1007
BstV2I GAAGAC 2 cut(s) 37, 733
BstXI CCANNNNNNTGG 1 cut(s) 1022
Bsu15I ATCGAT 1 cut(s) 474
Bsu36I CCTNAGG 1 cut(s) 499
BsuRI GGCC 6 cut(s) 89, 134, 152, 170, 317, 606
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 2 cut(s) 607, 889
BtrI CACGTC 2 cut(s) 487, 871
BtsCI GGATG 5 cut(s) 152, 374, 655, 885, 970
BtsIMutI CAGTG 4 cut(s) 294, 329, 959, 1027
Cfr13I GGNCC 4 cut(s) 150, 557, 599, 637
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 1 cut(s) 493
CsiI ACCWGGT 1 cut(s) 207
Csp6I GTAC 3 cut(s) 693, 735, 940
CviAII CATG 8 cut(s) 248, 594, 608, 720, 767, 775, 1008, 1016
CviQI GTAC 3 cut(s) 693, 735, 940
DdeI CTNAG 7 cut(s) 55, 499, 522, 707, 747, 977, 1032
DpnI GATC 2 cut(s) 807, 956
DpnII GATC 2 cut(s) 805, 954
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 3 cut(s) 87, 168, 604
Eco130I CCWWGG 2 cut(s) 129, 607
Eco147I AGGCCT 2 cut(s) 134, 317
Eco47I GGWCC 3 cut(s) 557, 599, 637
Eco81I CCTNAGG 1 cut(s) 499
Eco91I GGTNACC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 637
EcoO65I GGTNACC 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 276
EcoRII CCWGG 4 cut(s) 207, 639, 820, 838
EcoT14I CCWWGG 2 cut(s) 129, 607
ErhI CCWWGG 2 cut(s) 129, 607
FaeI CATG 8 cut(s) 251, 597, 611, 723, 770, 778, 1011, 1019
FaqI GGGAC 1 cut(s) 175
FatI CATG 8 cut(s) 247, 593, 607, 719, 766, 774, 1007, 1015
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 2 cut(s) 415, 996
FokI GGATG 5 cut(s) 159, 361, 662, 872, 977
Fsp4HI GCNGC 2 cut(s) 415, 996
FspBI CTAG 1 cut(s) 936
GluI GCNGC 2 cut(s) 415, 996
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 2 cut(s) 353, 662
HaeIII GGCC 6 cut(s) 89, 134, 152, 170, 317, 606
HapII CCGG 5 cut(s) 197, 270, 321, 560, 603
HgaI GACGC 1 cut(s) 493
Hin1I GRCGYC 1 cut(s) 504
Hin1II CATG 8 cut(s) 251, 597, 611, 723, 770, 778, 1011, 1019
HindIII AAGCTT 2 cut(s) 156, 1002
HinfI GANTC 5 cut(s) 122, 480, 518, 612, 711
HpaII CCGG 5 cut(s) 197, 270, 321, 560, 603
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 1 cut(s) 708
Hpy188III TCNNGA 6 cut(s) 144, 281, 361, 515, 622, 852
Hpy8I GTNNAC 1 cut(s) 599
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 4 cut(s) 545, 670, 679, 929
HpyCH4IV ACGT 3 cut(s) 486, 588, 870
HpyCH4V TGCA 6 cut(s) 71, 312, 417, 433, 530, 658
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 7 cut(s) 55, 499, 522, 707, 747, 977, 1032
HpySE526I ACGT 3 cut(s) 486, 588, 870
Hsp92I GRCGYC 1 cut(s) 504
Hsp92II CATG 8 cut(s) 251, 597, 611, 723, 770, 778, 1011, 1019
KpnI GGTACC 1 cut(s) 943
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 2 cut(s) 805, 954
LmnI GCTCC 1 cut(s) 98
Lsp1109I GCAGC 2 cut(s) 401, 1007
LweI GCATC 1 cut(s) 627
MabI ACCWGGT 1 cut(s) 207
MaeI CTAG 1 cut(s) 936
MaeII ACGT 3 cut(s) 486, 588, 870
MalI GATC 2 cut(s) 807, 956
MboI GATC 2 cut(s) 805, 954
MboII GAAGA 3 cut(s) 37, 212, 738
MfeI CAATTG 1 cut(s) 552
MhlI GDGCHC 1 cut(s) 839
MluCI AATT 5 cut(s) 276, 552, 811, 861, 920
MlyI GAGTC 2 cut(s) 116, 512
MmeI TCCRAC 2 cut(s) 366, 787
MnlI CCTC 9 cut(s) 29, 175, 186, 328, 463, 508, 637, 640, 1027
MslI CAYNNNNRTG 3 cut(s) 252, 359, 1020
MspA1I CMGCKG 1 cut(s) 414
MspI CCGG 5 cut(s) 197, 270, 321, 560, 603
MspR9I CCNGG 5 cut(s) 209, 603, 641, 822, 840
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 4 cut(s) 209, 641, 822, 840
MwoI GCNNNNNNNGC 1 cut(s) 1001
NciI CCSGG 1 cut(s) 603
NcoI CCATGG 1 cut(s) 607
NdeII GATC 2 cut(s) 805, 954
NlaIII CATG 8 cut(s) 251, 597, 611, 723, 770, 778, 1011, 1019
NlaIV GGNNCC 4 cut(s) 100, 601, 836, 941
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 6 cut(s) 370, 458, 505, 536, 866, 871
NspI RCATGY 3 cut(s) 251, 597, 770
PceI AGGCCT 2 cut(s) 134, 317
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 480, 612, 711
PflFI GACNNNGTC 1 cut(s) 545
PkrI GCNGC 2 cut(s) 416, 997
PleI GAGTC 2 cut(s) 116, 512
PpsI GAGTC 2 cut(s) 116, 512
PpuMI RGGWCCY 1 cut(s) 637
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 4 cut(s) 207, 639, 820, 838
PspEI GGTNACC 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 4 cut(s) 207, 639, 820, 838
PspN4I GGNNCC 4 cut(s) 100, 601, 836, 941
PspPI GGNCC 4 cut(s) 150, 557, 599, 637
PspPPI RGGWCCY 1 cut(s) 637
PsyI GACNNNGTC 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 414
RsaI GTAC 3 cut(s) 694, 736, 941
RsaNI GTAC 3 cut(s) 693, 735, 940
RseI CAYNNNNRTG 3 cut(s) 252, 359, 1020
SatI GCNGC 2 cut(s) 415, 996
Sau3AI GATC 2 cut(s) 805, 954
Sau96I GGNCC 4 cut(s) 150, 557, 599, 637
ScaI AGTACT 2 cut(s) 694, 736
SchI GAGTC 2 cut(s) 116, 512
ScrFI CCNGG 5 cut(s) 209, 603, 641, 822, 840
SduI GDGCHC 1 cut(s) 839
SexAI ACCWGGT 1 cut(s) 207
SfaNI GCATC 1 cut(s) 627
SfcI CTRYAG 1 cut(s) 928
SinI GGWCC 3 cut(s) 557, 599, 637
SmiMI CAYNNNNRTG 3 cut(s) 252, 359, 1020
Sse9I AATT 5 cut(s) 276, 552, 811, 861, 920
SseBI AGGCCT 2 cut(s) 134, 317
SspMI CTAG 1 cut(s) 936
StuI AGGCCT 2 cut(s) 134, 317
StyD4I CCNGG 5 cut(s) 207, 601, 639, 820, 838
StyI CCWWGG 2 cut(s) 129, 607
TaaI ACNGT 4 cut(s) 545, 670, 679, 929
TaiI ACGT 3 cut(s) 489, 591, 873
TaqI TCGA 4 cut(s) 347, 474, 516, 924
TasI AATT 5 cut(s) 276, 552, 811, 861, 920
TatI WGTACW 2 cut(s) 692, 734
TfiI GAWTC 3 cut(s) 480, 612, 711
TscAI CASTG 4 cut(s) 301, 336, 966, 1027
TseFI GTSAC 6 cut(s) 370, 458, 505, 536, 866, 871
TseI GCWGC 2 cut(s) 414, 995
Tsp45I GTSAC 6 cut(s) 370, 458, 505, 536, 866, 871
TspDTI ATGAA 2 cut(s) 37, 996
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 4 cut(s) 301, 336, 966, 1027
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 3 cut(s) 557, 599, 637
XagI CCTNNNNNAGG 1 cut(s) 644
XapI RAATTY 2 cut(s) 276, 920
XceI RCATGY 3 cut(s) 251, 597, 770
XspI CTAG 1 cut(s) 936
ZrmI AGTACT 2 cut(s) 694, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.