Rroxscaffold_2G00128640

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000702
Physical Location & Seq
Forward (+)
64395476 .. 64397103
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00128640.1

Sequence Viewer

Length: 1179 bp
ATGGCGAAATATCCCAGAAGCCACATTTTTCTCTCACTCCTCTTTCTCTTCATAAACTCCGGCGTCTCCACTGCAGTTACTTACAATGTGGCCGATTTAGGAGCCAAACCAGACGGCCAGACCGACTCAACCAAAGCCTTCCTTTCTGCATGGGCCGAAGCTTGTGCCTCGGCCAATCCCTCGGTTATTTATGTCCCGGCCGGAAGGTTCTTGCTTCGCAATGCAGTCTTCAGCGGACCATGTAAAAACATAGCCATCACATTTCGCATTGCCGGCACACTCGTGTCCCCGTCGGATTACCGGGTCATAGGAAATGCCGGAAACTGGCTTTTGTTTGAGCATGTGAATGGGATTACAATCTCGGGTGGAATTCTTGATGGACAAGGCACTGGTCTGTGGGATTGCAAAGCCTCCGGCAATAGTTGCCCCAGCGGAGCAACTACGCTTGGGTTTTCCAACTCCAACAACATTGTCGTGAGCGGATTGACCTCCCTAAACAGCCAAATGTTTCACATAGTCATCAACGGCTGCAACAACGTGAAAATGCAAGGTGTTAGGGTTTCTGCCTCCGGTGACAGCCCAAACACCGACGGAATCCACGTCCAACAGTCCTCGGGCGTCACTATTCTCGACTCTAAAATTGCAACCGGTGACGATTGTGTCTCAATTGGTCCTGGAACCACTAATTTGTGGATCGAAAACATTGCATGTGGACCAGGCCATGGTATCAGCATTGGAAGTCTAGGCAAGGACCAGCAGGAGGATGGTGTGCAAAATGTAACAGTGAAAACTGTTACATTTACTGGTACTCAAAATGGTGTGAGAATTAAATCATGGGGGAGACCTAGCACGGGGTTTGCCAAGAACATTCTTTTCCAACACGCTGTGATGGTCGATGTCCAAAATCCTATCATCATTGATCAAAATTACTGCCCTGGCGGGAAAGGTTGTCCTGGTCAGGTTTCGGGAGTTCAAATTAGCGATGTGACATATCAAGACATCCACGGGACATCAGCAACAGAAGTTGCGGTGAAATTCGACTGTAGTTCAAAGTATCCTTGTAACAAGATCACATTGAATGATGTGAAGCTCAATTACAAGAACCAAGCGGCCGAAGCTTCATGTAGCCATGCCGGTGGAACGGCTGAGGGTACGGTTCAGCCTACAAGTTGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

392

Amino Acids

40.96

Weight (kDa)

6.69

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 29 - 280 1.4e-14 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 55 - 376 1.5e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 480
AciI CCGC 6 cut(s) 234, 432, 480, 939, 1028, 1109
AclWI GGATC 1 cut(s) 701
AcoI YGGCCR 5 cut(s) 90, 115, 171, 198, 1110
AcsI RAATTY 2 cut(s) 369, 1034
AcuI CTGAAG 1 cut(s) 214
AcyI GRCGYC 2 cut(s) 63, 618
AdeI CACNNNGTG 1 cut(s) 886
AfaI GTAC 2 cut(s) 808, 1153
AfiI CCNNNNNNNGG 4 cut(s) 324, 722, 760, 851
AgeI ACCGGT 1 cut(s) 647
AgsI TTSAA 3 cut(s) 974, 1050, 1078
AjiI CACGTC 1 cut(s) 601
AjnI CCWGG 4 cut(s) 673, 715, 934, 952
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 3 cut(s) 161, 1090, 1118
AluI AGCT 3 cut(s) 161, 1090, 1118
Alw26I GTCTC 3 cut(s) 70, 667, 835
AlwI GGATC 1 cut(s) 701
Ama87I CYCGRG 2 cut(s) 361, 613
AoxI GGCC 7 cut(s) 90, 115, 153, 171, 198, 718, 1110
ApeKI GCWGC 1 cut(s) 528
ApoI RAATTY 2 cut(s) 369, 1034
AsiGI ACCGGT 1 cut(s) 647
AspS9I GGNCC 5 cut(s) 153, 236, 671, 713, 751
AsuC2I CCSGG 2 cut(s) 197, 302
AsuHPI GGTGA 3 cut(s) 584, 662, 1042
AvaI CYCGRG 2 cut(s) 361, 613
AvaII GGWCC 4 cut(s) 236, 671, 713, 751
BarI GAAGNNNNNNTAC 2 cut(s) 1079, 1111
BauI CACGAG 1 cut(s) 281
BbsI GAAGAC 1 cut(s) 220
BbvCI CCTCAGC 1 cut(s) 1146
BbvI GCAGC 1 cut(s) 515
BccI CCATC 4 cut(s) 263, 371, 758, 883
BceAI ACGGC 3 cut(s) 130, 541, 1158
BcgI CGANNNNNNTGC 4 cut(s) 146, 180, 686, 720
BciT130I CCWGG 4 cut(s) 675, 717, 936, 954
BciVI GTATCC 1 cut(s) 1065
BclI TGATCA 1 cut(s) 919
BcnI CCSGG 2 cut(s) 197, 302
BcoDI GTCTC 3 cut(s) 70, 667, 835
BfaI CTAG 2 cut(s) 743, 846
BfmI CTRYAG 3 cut(s) 72, 1042, 1175
BfuI GTATCC 1 cut(s) 1065
BisI GCNGC 2 cut(s) 529, 1110
BlsI GCNGC 2 cut(s) 530, 1111
Bme1390I CCNGG 6 cut(s) 197, 302, 675, 717, 936, 954
Bme18I GGWCC 4 cut(s) 236, 671, 713, 751
BmeT110I CYCGRG 2 cut(s) 361, 613
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 5 cut(s) 153, 236, 671, 713, 751
BmiI GGNNCC 2 cut(s) 103, 679
BmrFI CCNGG 6 cut(s) 197, 302, 675, 717, 936, 954
BpiI GAAGAC 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 1146
BpuMI CCSGG 2 cut(s) 197, 302
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 63, 618
BsaI GGTCTC 1 cut(s) 835
BsaJI CCNNGG 6 cut(s) 168, 180, 612, 721, 934, 1003
BsaWI WCCGGW 2 cut(s) 569, 647
Bsc4I CCNNNNNNNGG 4 cut(s) 324, 722, 760, 851
Bse118I RCCGGY 3 cut(s) 272, 647, 1133
Bse1I ACTGG 3 cut(s) 329, 394, 808
Bse3DI GCAATG 3 cut(s) 226, 267, 702
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 4 cut(s) 675, 717, 936, 954
BseDI CCNNGG 6 cut(s) 168, 180, 612, 721, 934, 1003
BseGI GGATG 2 cut(s) 769, 999
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 4 cut(s) 324, 722, 760, 851
BseMI GCAATG 3 cut(s) 226, 267, 702
BseMII CTCAG 1 cut(s) 1137
BseNI ACTGG 3 cut(s) 329, 394, 808
BseRI GAGGAG 1 cut(s) 29
BseX3I CGGCCG 2 cut(s) 198, 1110
BseXI GCAGC 1 cut(s) 515
BseYI CCCAGC 1 cut(s) 428
Bsh1285I CGRYCG 2 cut(s) 201, 1113
BshFI GGCC 7 cut(s) 92, 117, 155, 173, 200, 720, 1112
BshTI ACCGGT 1 cut(s) 647
BsiEI CGRYCG 2 cut(s) 201, 1113
BsiHKCI CYCGRG 2 cut(s) 361, 613
BslFI GGGAC 3 cut(s) 179, 271, 1021
BslI CCNNNNNNNGG 4 cut(s) 324, 722, 760, 851
BsmAI GTCTC 3 cut(s) 70, 667, 835
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 3 cut(s) 179, 271, 1021
BsnI GGCC 7 cut(s) 92, 117, 155, 173, 200, 720, 1112
Bso31I GGTCTC 1 cut(s) 835
BsoBI CYCGRG 2 cut(s) 361, 613
Bsp143I GATC 3 cut(s) 693, 919, 1068
Bsp19I CCATGG 1 cut(s) 721
BspACI CCGC 6 cut(s) 234, 432, 480, 939, 1028, 1109
BspANI GGCC 7 cut(s) 92, 117, 155, 173, 200, 720, 1112
BspCNI CTCAG 1 cut(s) 1138
BspLI GGNNCC 2 cut(s) 103, 679
BspMAI CTGCAG 1 cut(s) 76
BspPI GGATC 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 835
BsrBI CCGCTC 1 cut(s) 480
BsrDI GCAATG 3 cut(s) 226, 267, 702
BsrFI RCCGGY 3 cut(s) 272, 647, 1133
BsrI ACTGG 3 cut(s) 329, 394, 808
BssAI RCCGGY 3 cut(s) 272, 647, 1133
BssECI CCNNGG 6 cut(s) 168, 180, 612, 721, 934, 1003
BssMI GATC 3 cut(s) 693, 919, 1068
BssNI GRCGYC 2 cut(s) 63, 618
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 721
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 4 cut(s) 675, 717, 936, 954
Bst4CI ACNGT 5 cut(s) 609, 784, 793, 1043, 1156
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 2 cut(s) 63, 618
BstAPI GCANNNNNTGC 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 1 cut(s) 1146
BstDSI CCRYGG 2 cut(s) 721, 1003
BstF5I GGATG 2 cut(s) 769, 999
BstKTI GATC 3 cut(s) 696, 922, 1071
BstMAI GTCTC 3 cut(s) 70, 667, 835
BstMBI GATC 3 cut(s) 693, 919, 1068
BstMCI CGRYCG 2 cut(s) 201, 1113
BstMWI GCNNNNNNNGC 3 cut(s) 273, 423, 1115
BstNI CCWGG 4 cut(s) 675, 717, 936, 954
BstNSI RCATGY 2 cut(s) 344, 711
BstSCI CCNGG 6 cut(s) 195, 300, 673, 715, 934, 952
BstSFI CTRYAG 3 cut(s) 72, 1042, 1175
BstV1I GCAGC 1 cut(s) 515
BstV2I GAAGAC 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 1136
BstZI CGGCCG 2 cut(s) 198, 1110
BsuI GTATCC 1 cut(s) 1065
BsuRI GGCC 7 cut(s) 92, 117, 155, 173, 200, 720, 1112
BtgI CCRYGG 2 cut(s) 721, 1003
BtgZI GCGATG 1 cut(s) 996
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 2 cut(s) 769, 999
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 3 cut(s) 69, 387, 789
Cac8I GCNNGC 1 cut(s) 274
Cfr10I RCCGGY 3 cut(s) 272, 647, 1133
Cfr13I GGNCC 5 cut(s) 153, 236, 671, 713, 751
CseI GACGC 2 cut(s) 52, 607
Csp6I GTAC 2 cut(s) 807, 1152
CspAI ACCGGT 1 cut(s) 647
CviAII CATG 8 cut(s) 150, 240, 341, 708, 722, 834, 1122, 1130
CviQI GTAC 2 cut(s) 807, 1152
DdeI CTNAG 1 cut(s) 1146
DpnI GATC 3 cut(s) 695, 921, 1070
DpnII GATC 3 cut(s) 693, 919, 1068
DraIII CACNNNGTG 1 cut(s) 886
DrdI GACNNNNNNGTC 1 cut(s) 659
DseDI GACNNNNNNGTC 1 cut(s) 659
EaeI YGGCCR 5 cut(s) 90, 115, 171, 198, 1110
EagI CGGCCG 2 cut(s) 198, 1110
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EclXI CGGCCG 2 cut(s) 198, 1110
Eco130I CCWWGG 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 835
Eco47I GGWCC 4 cut(s) 236, 671, 713, 751
Eco52I CGGCCG 2 cut(s) 198, 1110
Eco57I CTGAAG 1 cut(s) 214
Eco88I CYCGRG 2 cut(s) 361, 613
EcoRI GAATTC 1 cut(s) 369
EcoRII CCWGG 4 cut(s) 673, 715, 934, 952
EcoT14I CCWWGG 1 cut(s) 721
ErhI CCWWGG 1 cut(s) 721
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 8 cut(s) 153, 243, 344, 711, 725, 837, 1125, 1133
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 3 cut(s) 179, 271, 1021
FatI CATG 8 cut(s) 149, 239, 340, 707, 721, 833, 1121, 1129
FauI CCCGC 1 cut(s) 932
FbaI TGATCA 1 cut(s) 919
Fnu4HI GCNGC 2 cut(s) 529, 1110
FokI GGATG 2 cut(s) 776, 986
Fsp4HI GCNGC 2 cut(s) 529, 1110
FspBI CTAG 2 cut(s) 743, 846
GluI GCNGC 2 cut(s) 529, 1110
GsaI CCCAGC 1 cut(s) 432
HaeIII GGCC 7 cut(s) 92, 117, 155, 173, 200, 720, 1112
HgaI GACGC 2 cut(s) 52, 607
Hin1I GRCGYC 2 cut(s) 63, 618
Hin1II CATG 8 cut(s) 153, 243, 344, 711, 725, 837, 1125, 1133
HindIII AAGCTT 2 cut(s) 159, 1116
HinfI GANTC 3 cut(s) 125, 594, 632
HphI GGTGA 3 cut(s) 584, 662, 1042
Hpy166II GTNNAC 1 cut(s) 713
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 5 cut(s) 374, 475, 629, 966, 995
Hpy8I GTNNAC 1 cut(s) 713
Hpy99I CGWCG 2 cut(s) 295, 593
HpyAV CCTTC 2 cut(s) 148, 198
HpyCH4III ACNGT 5 cut(s) 609, 784, 793, 1043, 1156
HpyCH4IV ACGT 2 cut(s) 537, 600
HpyCH4V TGCA 9 cut(s) 74, 149, 224, 405, 531, 547, 644, 707, 772
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 423, 1115
HpyF3I CTNAG 1 cut(s) 1146
HpySE526I ACGT 2 cut(s) 537, 600
Hsp92I GRCGYC 2 cut(s) 63, 618
Hsp92II CATG 8 cut(s) 153, 243, 344, 711, 725, 837, 1125, 1133
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 919
Kzo9I GATC 3 cut(s) 693, 919, 1068
LmnI GCTCC 2 cut(s) 101, 434
Lsp1109I GCAGC 1 cut(s) 515
MaeI CTAG 2 cut(s) 743, 846
MaeII ACGT 2 cut(s) 537, 600
MaeIII GTNAC 8 cut(s) 76, 572, 619, 650, 778, 793, 985, 1061
MalI GATC 3 cut(s) 695, 921, 1070
MbiI CCGCTC 1 cut(s) 480
MboI GATC 3 cut(s) 693, 919, 1068
MboII GAAGA 2 cut(s) 40, 220
MfeI CAATTG 1 cut(s) 666
MluCI AATT 9 cut(s) 369, 639, 666, 685, 825, 925, 975, 1034, 1093
MlyI GAGTC 2 cut(s) 119, 626
MmeI TCCRAC 5 cut(s) 273, 480, 486, 628, 901
MnlI CCTC 9 cut(s) 50, 178, 190, 421, 499, 577, 622, 754, 1141
MroNI GCCGGC 1 cut(s) 272
MseI TTAA 1 cut(s) 828
MslI CAYNNNNRTG 4 cut(s) 281, 345, 473, 1134
MspA1I CMGCKG 2 cut(s) 234, 432
MspR9I CCNGG 6 cut(s) 197, 302, 675, 717, 936, 954
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 4 cut(s) 675, 717, 936, 954
MwoI GCNNNNNNNGC 3 cut(s) 273, 423, 1115
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 2 cut(s) 197, 302
NcoI CCATGG 1 cut(s) 721
NdeII GATC 3 cut(s) 693, 919, 1068
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 8 cut(s) 153, 243, 344, 711, 725, 837, 1125, 1133
NlaIV GGNNCC 2 cut(s) 103, 679
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 4 cut(s) 572, 619, 650, 985
NspI RCATGY 2 cut(s) 344, 711
OliI CACNNNNGTG 1 cut(s) 281
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 597
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 594
PflMI CCANNNNNTGG 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 673
PinAI ACCGGT 1 cut(s) 647
PkrI GCNGC 2 cut(s) 530, 1111
PleI GAGTC 2 cut(s) 119, 626
PpsI GAGTC 2 cut(s) 119, 626
Psp6I CCWGG 4 cut(s) 673, 715, 934, 952
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 4 cut(s) 673, 715, 934, 952
PspN4I GGNNCC 2 cut(s) 103, 679
PspPI GGNCC 5 cut(s) 153, 236, 671, 713, 751
PstI CTGCAG 1 cut(s) 76
RsaI GTAC 2 cut(s) 808, 1153
RsaNI GTAC 2 cut(s) 807, 1152
RseI CAYNNNNRTG 4 cut(s) 281, 345, 473, 1134
SaqAI TTAA 1 cut(s) 828
SatI GCNGC 2 cut(s) 529, 1110
Sau3AI GATC 3 cut(s) 693, 919, 1068
Sau96I GGNCC 5 cut(s) 153, 236, 671, 713, 751
SchI GAGTC 2 cut(s) 119, 626
ScrFI CCNGG 6 cut(s) 197, 302, 675, 717, 936, 954
SfcI CTRYAG 3 cut(s) 72, 1042, 1175
SinI GGWCC 4 cut(s) 236, 671, 713, 751
SmiMI CAYNNNNRTG 4 cut(s) 281, 345, 473, 1134
Sse9I AATT 9 cut(s) 369, 639, 666, 685, 825, 925, 975, 1034, 1093
SsiI CCGC 6 cut(s) 234, 432, 480, 939, 1028, 1109
SspMI CTAG 2 cut(s) 743, 846
StyD4I CCNGG 6 cut(s) 195, 300, 673, 715, 934, 952
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 5 cut(s) 609, 784, 793, 1043, 1156
TaiI ACGT 2 cut(s) 540, 603
TaqI TCGA 4 cut(s) 630, 696, 894, 1038
TaqII GACCGA 1 cut(s) 137
TasI AATT 9 cut(s) 369, 639, 666, 685, 825, 925, 975, 1034, 1093
TauI GCSGC 1 cut(s) 1112
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 1 cut(s) 828
Tru9I TTAA 1 cut(s) 828
TscAI CASTG 3 cut(s) 76, 394, 789
TseFI GTSAC 4 cut(s) 572, 619, 650, 985
TseI GCWGC 1 cut(s) 528
Tsp45I GTSAC 4 cut(s) 572, 619, 650, 985
TspDTI ATGAA 2 cut(s) 40, 1110
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 3 cut(s) 76, 394, 789
Van91I CCANNNNNTGG 1 cut(s) 722
VpaK11BI GGWCC 4 cut(s) 236, 671, 713, 751
XapI RAATTY 2 cut(s) 369, 1034
XceI RCATGY 2 cut(s) 344, 711
XcmI CCANNNNNNNNNTGG 1 cut(s) 761
XspI CTAG 2 cut(s) 743, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.