Rh2BG003000

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000702
Physical Location & Seq
Forward (+)
285086 .. 287130
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG003000.1

Sequence Viewer

Length: 1119 bp
ATGGCCAAGGCAGTCACTTACAGCGTGACCACCTTAGGAGCCAAACCAGATGGCAAGACCGACTCAACTAAGGCCTTCCTTTCTGCATGGGCTAAAGCTTGTGCCTCGGTGAAACCAGCTGTGATTTACGTGCCGGCAGGGAGGTTCTTGCTCCGCAATGCCCTGTTCAGCGGACCATGCAAGAACAGCCTCATCACCTTCCGCATTGCTGGCACCCTCGTTGCTCCGTCCGATTACCGGGTCATTGGAAATGCCGGTAACTGGCTCCTCTTTCGGCAAGTCAACGGTGTTACCATTTCCGGTGGAATTCTTGACGGTCAAGGCACTGGTTTGTGGGCGTGCAAGAACTCCGGCAAGAGTTGCCCCACTGGAGCCACGACATTGAGTTTTTCCAACTCCAACAATATTGTGGTTAGCGGATTGGTATCTCTAAACAGCCAAATGTTTCACATAGTCATCAACGGTTGCAACAATGTTAAAATGCAAGGGGTTAAAGTATCAGCCTCCGGCAACAGCCCTAACACCGACGGTATCCATGTTCAAATGTCGTCGGGTGTTACCATTCTCAACTCAAAGATCTCTACCGGTGATGATTGTGTATCAATTGGTCCCGGCACTTCTAACTTGTGGATTGAAAATGTTGCATGCGGACCGGGTCACGGAATTAGCATTGGGAGTCTAGGCAAAGACCTACAGGAAGCTGGTGTGCAAAATGTAACGGTCAAAACAGTGACATTTACCAATACTCAAAATGGTGTGAGGATCAAGTCCTGGGGGAGGGCTAGCACTGGATTTGCCAGGAACATTCTTTTCCAACATGCTGTCATGATTAACGTTCAAAATCCGATAGTCATCGATCAACAATATTGTCCCGACAAGAAAGGCTGCCCTGGCCAGGTTTCTGGAGTTAAAATCAGTGATGTGACATATCAAGACATTCATGGTACATCGGCAACAGAAGTTGCAGTGAAATTCGATTGTAGTTCAAAGTACCCATGTAGCAGAATCAGATTAGAGAATGTGAAGCTCATTTACAAGAATAACCAAGGAGCTCTAGCTTCATGTAGCAATGCTGGTGGAACATCTGCGGGTACGGTGCAGCCTACAAGTTGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

372

Amino Acids

38.85

Weight (kDa)

9.33

Isoelectric Point (pI)

26.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 8 - 259 2.4e-14 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 34 - 358 1.7e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 212
AciI CCGC 6 cut(s) 154, 171, 202, 417, 648, 1088
AclI AACGTT 1 cut(s) 834
AclWI GGATC 1 cut(s) 770
AcoI YGGCCR 2 cut(s) 3, 892
AcsI RAATTY 2 cut(s) 306, 971
AfaI GTAC 3 cut(s) 946, 992, 1093
AfiI CCNNNNNNNGG 5 cut(s) 237, 261, 659, 777, 895
AgeI ACCGGT 1 cut(s) 584
AgsI TTSAA 4 cut(s) 542, 635, 839, 987
AjnI CCWGG 4 cut(s) 770, 797, 889, 894
AluBI AGCT 6 cut(s) 98, 119, 701, 1027, 1052, 1058
AluI AGCT 6 cut(s) 98, 119, 701, 1027, 1052, 1058
Alw21I GWGCWC 1 cut(s) 1054
AlwI GGATC 1 cut(s) 770
AoxI GGCC 3 cut(s) 3, 72, 892
ApeKI GCWGC 2 cut(s) 885, 1099
ApoI RAATTY 2 cut(s) 306, 971
AsiGI ACCGGT 1 cut(s) 584
AspS9I GGNCC 3 cut(s) 173, 608, 650
AsuC2I CCSGG 3 cut(s) 239, 612, 654
AsuHPI GGTGA 3 cut(s) 121, 187, 599
AsuNHI GCTAGC 1 cut(s) 782
AvaII GGWCC 3 cut(s) 173, 608, 650
AxyI CCTNAGG 1 cut(s) 34
BalI TGGCCA 2 cut(s) 5, 894
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 1 cut(s) 1054
BarI GAAGNNNNNNTAC 2 cut(s) 1016, 1048
Bbv12I GWGCWC 1 cut(s) 1054
BbvI GCAGC 2 cut(s) 872, 1111
BccI CCATC 1 cut(s) 44
BciT130I CCWGG 4 cut(s) 772, 799, 891, 896
BciVI GTATCC 1 cut(s) 542
BcnI CCSGG 3 cut(s) 239, 612, 654
BfaI CTAG 3 cut(s) 680, 783, 1055
BfmI CTRYAG 2 cut(s) 692, 1115
BfuI GTATCC 1 cut(s) 542
BglII AGATCT 1 cut(s) 576
BisI GCNGC 2 cut(s) 886, 1100
BlsI GCNGC 2 cut(s) 887, 1101
Bme1390I CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
Bme18I GGWCC 3 cut(s) 173, 608, 650
BmgT120I GGNCC 3 cut(s) 173, 608, 650
BmiI GGNNCC 5 cut(s) 40, 214, 266, 373, 610
BmrFI CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
BmtI GCTAGC 1 cut(s) 786
BpmI CTGGAG 2 cut(s) 390, 924
BpuMI CCSGG 3 cut(s) 239, 612, 654
Bsa29I ATCGAT 1 cut(s) 855
BsaAI YACGTR 1 cut(s) 130
BsaBI GATNNNNATC 2 cut(s) 424, 851
BsaJI CCNNGG 5 cut(s) 6, 105, 771, 889, 1045
BsaWI WCCGGW 2 cut(s) 299, 584
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 261, 659, 777, 895
Bse118I RCCGGY 3 cut(s) 133, 254, 584
Bse1I ACTGG 4 cut(s) 266, 331, 373, 793
Bse21I CCTNAGG 1 cut(s) 34
Bse3DI GCAATG 3 cut(s) 163, 204, 1075
Bse8I GATNNNNATC 2 cut(s) 424, 851
BseBI CCWGG 4 cut(s) 772, 799, 891, 896
BseCI ATCGAT 1 cut(s) 855
BseDI CCNNGG 5 cut(s) 6, 105, 771, 889, 1045
BseJI GATNNNNATC 2 cut(s) 424, 851
BseLI CCNNNNNNNGG 5 cut(s) 237, 261, 659, 777, 895
BseMI GCAATG 3 cut(s) 163, 204, 1075
BseNI ACTGG 4 cut(s) 266, 331, 373, 793
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 872, 1111
BsgI GTGCAG 1 cut(s) 1118
BshFI GGCC 3 cut(s) 5, 74, 894
BshNI GGYRCC 1 cut(s) 212
BshTI ACCGGT 1 cut(s) 584
BshVI ATCGAT 1 cut(s) 855
BsiHKAI GWGCWC 1 cut(s) 1054
BsiSI CCGG 9 cut(s) 134, 238, 255, 300, 351, 507, 585, 612, 653
BslFI GGGAC 2 cut(s) 594, 855
BslI CCNNNNNNNGG 5 cut(s) 237, 261, 659, 777, 895
BsmFI GGGAC 2 cut(s) 594, 855
BsnI GGCC 3 cut(s) 5, 74, 894
Bsp1286I GDGCHC 1 cut(s) 1054
Bsp143I GATC 3 cut(s) 576, 762, 856
BspACI CCGC 6 cut(s) 154, 171, 202, 417, 648, 1088
BspANI GGCC 3 cut(s) 5, 74, 894
BspDI ATCGAT 1 cut(s) 855
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 5 cut(s) 40, 214, 266, 373, 610
BspOI GCTAGC 1 cut(s) 786
BspPI GGATC 1 cut(s) 770
BspT107I GGYRCC 1 cut(s) 212
BsrDI GCAATG 3 cut(s) 163, 204, 1075
BsrFI RCCGGY 3 cut(s) 133, 254, 584
BsrI ACTGG 4 cut(s) 266, 331, 373, 793
BssAI RCCGGY 3 cut(s) 133, 254, 584
BssECI CCNNGG 5 cut(s) 6, 105, 771, 889, 1045
BssMI GATC 3 cut(s) 576, 762, 856
BssT1I CCWWGG 2 cut(s) 6, 1045
Bst2UI CCWGG 4 cut(s) 772, 799, 891, 896
Bst4CI ACNGT 7 cut(s) 287, 317, 464, 530, 721, 730, 1096
BstAPI GCANNNNNTGC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 5 cut(s) 135, 211, 340, 646, 784
BstDEI CTNAG 2 cut(s) 34, 69
BstENI CCTNNNNNAGG 1 cut(s) 775
BstKTI GATC 3 cut(s) 579, 765, 859
BstMBI GATC 3 cut(s) 576, 762, 856
BstMWI GCNNNNNNNGC 5 cut(s) 177, 186, 210, 360, 891
BstNI CCWGG 4 cut(s) 772, 799, 891, 896
BstNSI RCATGY 2 cut(s) 648, 821
BstSCI CCNGG 7 cut(s) 237, 610, 652, 770, 797, 889, 894
BstSFI CTRYAG 2 cut(s) 692, 1115
BstV1I GCAGC 2 cut(s) 872, 1111
BstX2I RGATCY 1 cut(s) 576
BstXI CCANNNNNNTGG 1 cut(s) 902
BstYI RGATCY 1 cut(s) 576
Bsu15I ATCGAT 1 cut(s) 855
Bsu36I CCTNAGG 1 cut(s) 34
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 3 cut(s) 5, 74, 894
BsuTUI ATCGAT 1 cut(s) 855
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 6 cut(s) 324, 366, 735, 786, 922, 972
Cac8I GCNNGC 5 cut(s) 135, 211, 340, 646, 784
CciI TCATGA 1 cut(s) 825
Cfr10I RCCGGY 3 cut(s) 133, 254, 584
Cfr13I GGNCC 3 cut(s) 173, 608, 650
ClaI ATCGAT 1 cut(s) 855
CpoI CGGWCCG 1 cut(s) 650
Csp6I GTAC 3 cut(s) 945, 991, 1092
CspAI ACCGGT 1 cut(s) 584
CspCI CAANNNNNGTGG 2 cut(s) 1057, 1092
CspI CGGWCCG 1 cut(s) 650
CviAII CATG 9 cut(s) 87, 177, 536, 645, 818, 826, 941, 996, 1062
CviQI GTAC 3 cut(s) 945, 991, 1092
DdeI CTNAG 2 cut(s) 34, 69
DpnI GATC 3 cut(s) 578, 764, 858
DpnII GATC 3 cut(s) 576, 762, 856
EaeI YGGCCR 2 cut(s) 3, 892
Ecl136II GAGCTC 1 cut(s) 1052
Eco130I CCWWGG 2 cut(s) 6, 1045
Eco147I AGGCCT 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 1054
Eco47I GGWCC 3 cut(s) 173, 608, 650
Eco53kI GAGCTC 1 cut(s) 1052
Eco81I CCTNAGG 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 1052
EcoNI CCTNNNNNAGG 1 cut(s) 775
EcoRI GAATTC 1 cut(s) 306
EcoRII CCWGG 4 cut(s) 770, 797, 889, 894
EcoT14I CCWWGG 2 cut(s) 6, 1045
EcoT38I GRGCYC 1 cut(s) 1054
ErhI CCWWGG 2 cut(s) 6, 1045
FaeI CATG 9 cut(s) 90, 180, 539, 648, 821, 829, 944, 999, 1065
FaqI GGGAC 2 cut(s) 594, 855
FatI CATG 9 cut(s) 86, 176, 535, 644, 817, 825, 940, 995, 1061
FauI CCCGC 1 cut(s) 1081
Fnu4HI GCNGC 2 cut(s) 886, 1100
FriOI GRGCYC 1 cut(s) 1054
Fsp4HI GCNGC 2 cut(s) 886, 1100
FspBI CTAG 3 cut(s) 680, 783, 1055
GluI GCNGC 2 cut(s) 886, 1100
GsuI CTGGAG 2 cut(s) 390, 924
HaeIII GGCC 3 cut(s) 5, 74, 894
HapII CCGG 9 cut(s) 134, 238, 255, 300, 351, 507, 585, 612, 653
Hin1II CATG 9 cut(s) 90, 180, 539, 648, 821, 829, 944, 999, 1065
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 3 cut(s) 62, 676, 1005
HpaII CCGG 9 cut(s) 134, 238, 255, 300, 351, 507, 585, 612, 653
HphI GGTGA 3 cut(s) 121, 187, 599
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 3 cut(s) 232, 846, 1010
Hpy188III TCNNGA 5 cut(s) 311, 826, 872, 903, 932
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 2 cut(s) 530, 553
HpyAV CCTTC 2 cut(s) 85, 208
HpyCH4III ACNGT 7 cut(s) 287, 317, 464, 530, 721, 730, 1096
HpyCH4IV ACGT 2 cut(s) 129, 834
HpyCH4V TGCA 9 cut(s) 86, 180, 342, 468, 484, 644, 709, 965, 1099
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 186, 210, 360, 891
HpyF3I CTNAG 2 cut(s) 34, 69
HpySE526I ACGT 2 cut(s) 129, 834
Hsp92II CATG 9 cut(s) 90, 180, 539, 648, 821, 829, 944, 999, 1065
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 3 cut(s) 576, 762, 856
LmnI GCTCC 6 cut(s) 38, 156, 229, 270, 371, 1049
Lsp1109I GCAGC 2 cut(s) 872, 1111
MaeI CTAG 3 cut(s) 680, 783, 1055
MaeII ACGT 2 cut(s) 129, 834
MaeIII GTNAC 9 cut(s) 13, 25, 257, 289, 556, 656, 715, 730, 922
MalI GATC 3 cut(s) 578, 764, 858
MboI GATC 3 cut(s) 576, 762, 856
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 1 cut(s) 1054
MlsI TGGCCA 2 cut(s) 5, 894
MluCI AATT 4 cut(s) 306, 603, 663, 971
MluNI TGGCCA 2 cut(s) 5, 894
MlyI GAGTC 2 cut(s) 56, 685
MmeI TCCRAC 3 cut(s) 417, 423, 838
MnlI CCTC 8 cut(s) 115, 135, 200, 227, 278, 514, 753, 771
Mox20I TGGCCA 2 cut(s) 5, 894
MroNI GCCGGC 1 cut(s) 133
MscI TGGCCA 2 cut(s) 5, 894
MseI TTAA 4 cut(s) 477, 492, 831, 909
Msp20I TGGCCA 2 cut(s) 5, 894
MspA1I CMGCKG 2 cut(s) 119, 171
MspI CCGG 9 cut(s) 134, 238, 255, 300, 351, 507, 585, 612, 653
MspR9I CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
MunI CAATTG 1 cut(s) 603
MvaI CCWGG 4 cut(s) 772, 799, 891, 896
MwoI GCNNNNNNNGC 5 cut(s) 177, 186, 210, 360, 891
NaeI GCCGGC 1 cut(s) 135
NciI CCSGG 3 cut(s) 239, 612, 654
NdeII GATC 3 cut(s) 576, 762, 856
NgoMIV GCCGGC 1 cut(s) 133
NheI GCTAGC 1 cut(s) 782
NlaIII CATG 9 cut(s) 90, 180, 539, 648, 821, 829, 944, 999, 1065
NlaIV GGNNCC 5 cut(s) 40, 214, 266, 373, 610
NmuCI GTSAC 5 cut(s) 13, 25, 656, 730, 922
NspI RCATGY 2 cut(s) 648, 821
PaeI GCATGC 1 cut(s) 648
PagI TCATGA 1 cut(s) 825
PceI AGGCCT 1 cut(s) 74
PdiI GCCGGC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 1005
PflFI GACNNNGTC 1 cut(s) 654
PinAI ACCGGT 1 cut(s) 584
PkrI GCNGC 2 cut(s) 887, 1101
PleI GAGTC 2 cut(s) 56, 684
PpsI GAGTC 2 cut(s) 56, 684
Ppu21I YACGTR 1 cut(s) 130
Psp124BI GAGCTC 1 cut(s) 1054
Psp1406I AACGTT 1 cut(s) 834
Psp6I CCWGG 4 cut(s) 770, 797, 889, 894
PspGI CCWGG 4 cut(s) 770, 797, 889, 894
PspN4I GGNNCC 5 cut(s) 40, 214, 266, 373, 610
PspPI GGNCC 3 cut(s) 173, 608, 650
PsuI RGATCY 1 cut(s) 576
PsyI GACNNNGTC 1 cut(s) 654
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 3 cut(s) 946, 992, 1093
RsaNI GTAC 3 cut(s) 945, 991, 1092
Rsr2I CGGWCCG 1 cut(s) 650
RsrII CGGWCCG 1 cut(s) 650
SacI GAGCTC 1 cut(s) 1054
SaqAI TTAA 4 cut(s) 477, 492, 831, 909
SatI GCNGC 2 cut(s) 886, 1100
Sau3AI GATC 3 cut(s) 576, 762, 856
Sau96I GGNCC 3 cut(s) 173, 608, 650
SchI GAGTC 2 cut(s) 56, 685
ScrFI CCNGG 7 cut(s) 239, 612, 654, 772, 799, 891, 896
SduI GDGCHC 1 cut(s) 1054
SfcI CTRYAG 2 cut(s) 692, 1115
SinI GGWCC 3 cut(s) 173, 608, 650
SphI GCATGC 1 cut(s) 648
Sse9I AATT 4 cut(s) 306, 603, 663, 971
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 6 cut(s) 154, 171, 202, 417, 648, 1088
SspI AATATT 2 cut(s) 406, 866
SspMI CTAG 3 cut(s) 680, 783, 1055
SstI GAGCTC 1 cut(s) 1054
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 7 cut(s) 237, 610, 652, 770, 797, 889, 894
StyI CCWWGG 2 cut(s) 6, 1045
TaaI ACNGT 7 cut(s) 287, 317, 464, 530, 721, 730, 1096
TaiI ACGT 2 cut(s) 132, 837
TaqI TCGA 2 cut(s) 855, 975
TaqII GACCGA 1 cut(s) 74
TasI AATT 4 cut(s) 306, 603, 663, 971
TfiI GAWTC 1 cut(s) 1005
Tru1I TTAA 4 cut(s) 477, 492, 831, 909
Tru9I TTAA 4 cut(s) 477, 492, 831, 909
TscAI CASTG 6 cut(s) 331, 373, 735, 793, 922, 972
TseFI GTSAC 5 cut(s) 13, 25, 656, 730, 922
TseI GCWGC 2 cut(s) 885, 1099
Tsp45I GTSAC 5 cut(s) 13, 25, 656, 730, 922
TspDTI ATGAA 2 cut(s) 929, 1050
TspGWI ACGGA 2 cut(s) 216, 675
TspRI CASTG 6 cut(s) 331, 373, 735, 793, 922, 972
Tth111I GACNNNGTC 1 cut(s) 654
VpaK11BI GGWCC 3 cut(s) 173, 608, 650
XagI CCTNNNNNAGG 1 cut(s) 775
XapI RAATTY 2 cut(s) 306, 971
XceI RCATGY 2 cut(s) 648, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 3 cut(s) 680, 783, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.