Prupe.4G261700_v2.0.a1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000702
Physical Location & Seq
Forward (+)
19022760 .. 19024278
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G261700.1

Sequence Viewer

Length: 1179 bp
ATGGCAAACTCCACAAGCCTCATTGTTCTCTCACTTGCTTTTGTCTTCATGATTAACTCAGCCTTAGCCGCTAAAGCCCCATACAATGTGGCCAGTTTAGGAGGCAAAGGAGATGGGAAGACTGAGTCAACCAAAGCCTTCATTTCTGCATGGGCCAAAGCTTGTGCCTCGGCGGAGCCGGCTGTCATTTACGTGCCGAGGGGGAGGTTCTTGCTGCACAATGTAGTGTTCGCCGGGCCTTGCAAAAATAACGCAATCACCTTCCGCATTGCCGGCACTCTTGTAGCCCCATCCGATTACCGGGTCATTGGAAATGCCGATAACTGGATTTCCTTTGAGGATGTGGAGGGGGTTACTATATCTGGTGGAATTCTTGATGGCAAAGGCACCGACTTGTGGGCTTGCAAGGCAGCAGGCAAGAGTTGCCCCAGTGGAGCAACGACATTGAGTTTTTCTAACTCCAACGACATCAAGGTTAGTGGCGTGATATCCCTAAACAGCCAAATGTTCCACATAGTCATCAACGGCTGCCATAATGTGAAAATGCAAAGTGTTAAAGTTTCTGCCTCCGGGCAGAGCCCCAACACTGATGGCATTCACGTCCAGATGTCATCCAGCGTCACGATTCTCAACTCGAAGATCGCAACGGGTGACGACTGTGTCTCAATCGGTCCCGGTACTTCAGATTTGTGGATCGAAAACATTAAATGTGGACCCGGCCATGGAATCAGCATTGGGAGTCTAGGAAAGGACCAAGAAGAGGCTGGTGTGCAAAATGTAACAGTTAAATCGATTACCTTTTTCAATACTCAAAATGGTTTGAGGATCAAATCGTGGGGGAGGCCTAGCACTGGATTTGCTAAGGACATTCTTTTCCAACATGCTGTAATGCTCAATGTCCAAAATCCGATTGTTATCGATCAAAATTATTGCCCGGACAACAAAGGCTGCCCTGGTCAGGTCTCTGGAGTTAAAATCAGCGATGTGACATACCAAGACATCCATGGTACATCGGCAACAGAAGTTGCAGTTAAGTTCGATTGTAGTTCCAAGTACCCATGTAGCAATATCAAAATGAAGGATGTGAAGCTCACTTACAAGAATCAAGCAGCTGAAGCTTCATGTAGCCATGCAGCTGGAACATCTCATGGGACGGTGCAACCTACAAGTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

393

Amino Acids

41.15

Weight (kDa)

8.35

Isoelectric Point (pI)

32.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 659
AccB1I GGYRCC 1 cut(s) 386
AciI CCGC 3 cut(s) 69, 173, 265
AclWI GGATC 2 cut(s) 701, 833
AcoI YGGCCR 2 cut(s) 90, 718
AcsI RAATTY 1 cut(s) 369
AcuI CTGAAG 2 cut(s) 666, 1134
AfaI GTAC 3 cut(s) 679, 1009, 1055
AfiI CCNNNNNNNGG 7 cut(s) 300, 324, 396, 722, 760, 851, 958
AgsI TTSAA 1 cut(s) 805
AjiI CACGTC 1 cut(s) 601
AjnI CCWGG 1 cut(s) 952
AluBI AGCT 5 cut(s) 161, 1090, 1112, 1118, 1136
AluI AGCT 5 cut(s) 161, 1090, 1112, 1118, 1136
Alw26I GTCTC 2 cut(s) 667, 967
AlwI GGATC 2 cut(s) 701, 833
AoxI GGCC 5 cut(s) 90, 153, 236, 718, 842
ApeKI GCWGC 6 cut(s) 214, 410, 528, 948, 1109, 1133
ApoI RAATTY 1 cut(s) 369
AspS9I GGNCC 5 cut(s) 153, 236, 671, 713, 751
AsuC2I CCSGG 6 cut(s) 235, 302, 571, 675, 717, 935
AsuHPI GGTGA 2 cut(s) 250, 662
AvaII GGWCC 3 cut(s) 671, 713, 751
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 581
BarI GAAGNNNNNNTAC 2 cut(s) 1079, 1111
BbsI GAAGAC 2 cut(s) 37, 125
BbvI GCAGC 6 cut(s) 201, 422, 515, 935, 1121, 1145
BccI CCATC 4 cut(s) 107, 298, 371, 584
BceAI ACGGC 1 cut(s) 541
BciT130I CCWGG 1 cut(s) 954
BcnI CCSGG 6 cut(s) 235, 302, 571, 675, 717, 935
BcoDI GTCTC 2 cut(s) 667, 967
BfaI CTAG 2 cut(s) 743, 846
BisI GCNGC 7 cut(s) 69, 215, 411, 529, 949, 1110, 1134
BlsI GCNGC 7 cut(s) 70, 216, 412, 530, 950, 1111, 1135
Bme1390I CCNGG 7 cut(s) 235, 302, 571, 675, 717, 935, 954
Bme18I GGWCC 3 cut(s) 671, 713, 751
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 5 cut(s) 153, 236, 671, 713, 751
BmiI GGNNCC 4 cut(s) 177, 388, 673, 715
BmrFI CCNGG 7 cut(s) 235, 302, 571, 675, 717, 935, 954
BmrI ACTGGG 1 cut(s) 423
BmuI ACTGGG 1 cut(s) 423
BpiI GAAGAC 2 cut(s) 37, 125
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 2 cut(s) 64, 861
BpuMI CCSGG 6 cut(s) 235, 302, 571, 675, 717, 935
Bsa29I ATCGAT 2 cut(s) 791, 918
BsaAI YACGTR 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 914
BsaI GGTCTC 1 cut(s) 967
BsaJI CCNNGG 5 cut(s) 168, 197, 721, 952, 1003
Bsc4I CCNNNNNNNGG 7 cut(s) 300, 324, 396, 722, 760, 851, 958
Bse118I RCCGGY 2 cut(s) 178, 272
Bse1I ACTGG 4 cut(s) 93, 329, 429, 856
Bse3DI GCAATG 1 cut(s) 267
Bse8I GATNNNNATC 1 cut(s) 914
BseBI CCWGG 1 cut(s) 954
BseCI ATCGAT 2 cut(s) 791, 918
BseDI CCNNGG 5 cut(s) 168, 197, 721, 952, 1003
BseGI GGATG 5 cut(s) 290, 346, 611, 999, 1087
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 7 cut(s) 300, 324, 396, 722, 760, 851, 958
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 2 cut(s) 72, 114
BseNI ACTGG 4 cut(s) 93, 329, 429, 856
BseXI GCAGC 6 cut(s) 201, 422, 515, 935, 1121, 1145
BsgI GTGCAG 1 cut(s) 200
BshFI GGCC 5 cut(s) 92, 155, 238, 720, 844
BshNI GGYRCC 1 cut(s) 386
BshVI ATCGAT 2 cut(s) 791, 918
BsiSI CCGG 8 cut(s) 179, 234, 273, 301, 570, 675, 717, 935
BslFI GGGAC 2 cut(s) 657, 1165
BslI CCNNNNNNNGG 7 cut(s) 300, 324, 396, 722, 760, 851, 958
BsmAI GTCTC 2 cut(s) 667, 967
BsmFI GGGAC 2 cut(s) 657, 1165
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 5 cut(s) 92, 155, 238, 720, 844
Bso31I GGTCTC 1 cut(s) 967
Bsp1286I GDGCHC 1 cut(s) 581
Bsp143I GATC 4 cut(s) 639, 693, 825, 919
Bsp19I CCATGG 2 cut(s) 721, 1003
BspACI CCGC 3 cut(s) 69, 173, 265
BspANI GGCC 5 cut(s) 92, 155, 238, 720, 844
BspCNI CTCAG 2 cut(s) 71, 115
BspDI ATCGAT 2 cut(s) 791, 918
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 4 cut(s) 177, 388, 673, 715
BspPI GGATC 2 cut(s) 701, 833
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 967
BsrDI GCAATG 1 cut(s) 267
BsrFI RCCGGY 2 cut(s) 178, 272
BsrI ACTGG 4 cut(s) 93, 329, 429, 856
BssAI RCCGGY 2 cut(s) 178, 272
BssECI CCNNGG 5 cut(s) 168, 197, 721, 952, 1003
BssMI GATC 4 cut(s) 639, 693, 825, 919
BssT1I CCWWGG 2 cut(s) 721, 1003
Bst2UI CCWGG 1 cut(s) 954
Bst4CI ACNGT 3 cut(s) 659, 784, 1156
Bst6I CTCTTC 1 cut(s) 753
BstAPI GCANNNNNTGC 1 cut(s) 423
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 4 cut(s) 180, 274, 403, 415
BstDEI CTNAG 4 cut(s) 58, 64, 123, 861
BstDSI CCRYGG 2 cut(s) 721, 1003
BstF5I GGATG 5 cut(s) 290, 346, 611, 999, 1087
BstKTI GATC 4 cut(s) 642, 696, 828, 922
BstMAI GTCTC 2 cut(s) 667, 967
BstMBI GATC 4 cut(s) 639, 693, 825, 919
BstMWI GCNNNNNNNGC 7 cut(s) 68, 74, 179, 273, 407, 423, 1115
BstNI CCWGG 1 cut(s) 954
BstNSI RCATGY 1 cut(s) 884
BstSCI CCNGG 7 cut(s) 233, 300, 569, 673, 715, 933, 952
BstV1I GCAGC 6 cut(s) 201, 422, 515, 935, 1121, 1145
BstV2I GAAGAC 2 cut(s) 37, 125
BstXI CCANNNNNNTGG 1 cut(s) 1136
Bsu15I ATCGAT 2 cut(s) 791, 918
BsuRI GGCC 5 cut(s) 92, 155, 238, 720, 844
BsuTUI ATCGAT 2 cut(s) 791, 918
BtgI CCRYGG 2 cut(s) 721, 1003
BtgZI GCGATG 1 cut(s) 996
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 5 cut(s) 290, 346, 611, 999, 1087
BtsIMutI CAGTG 3 cut(s) 436, 585, 849
Cac8I GCNNGC 4 cut(s) 180, 274, 403, 415
CciI TCATGA 1 cut(s) 48
Cfr10I RCCGGY 2 cut(s) 178, 272
Cfr13I GGNCC 5 cut(s) 153, 236, 671, 713, 751
ClaI ATCGAT 2 cut(s) 791, 918
CseI GACGC 1 cut(s) 607
Csp6I GTAC 3 cut(s) 678, 1008, 1054
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviAII CATG 9 cut(s) 49, 150, 722, 881, 1004, 1059, 1122, 1130, 1148
CviQI GTAC 3 cut(s) 678, 1008, 1054
DdeI CTNAG 4 cut(s) 58, 64, 123, 861
DpnI GATC 4 cut(s) 641, 695, 827, 921
DpnII GATC 4 cut(s) 639, 693, 825, 919
DrdI GACNNNNNNGTC 1 cut(s) 659
DseDI GACNNNNNNGTC 1 cut(s) 659
EaeI YGGCCR 2 cut(s) 90, 718
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
EciI GGCGGA 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 721, 1003
Eco147I AGGCCT 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 581
Eco31I GGTCTC 1 cut(s) 967
Eco32I GATATC 1 cut(s) 489
Eco47I GGWCC 3 cut(s) 671, 713, 751
Eco57I CTGAAG 2 cut(s) 666, 1134
EcoRI GAATTC 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 952
EcoRV GATATC 1 cut(s) 489
EcoT14I CCWWGG 2 cut(s) 721, 1003
EcoT38I GRGCYC 1 cut(s) 581
ErhI CCWWGG 2 cut(s) 721, 1003
FaeI CATG 9 cut(s) 52, 153, 725, 884, 1007, 1062, 1125, 1133, 1151
FaqI GGGAC 2 cut(s) 657, 1165
FatI CATG 9 cut(s) 48, 149, 721, 880, 1003, 1058, 1121, 1129, 1147
Fnu4HI GCNGC 7 cut(s) 69, 215, 411, 529, 949, 1110, 1134
FokI GGATG 5 cut(s) 277, 353, 598, 986, 1094
FriOI GRGCYC 1 cut(s) 581
Fsp4HI GCNGC 7 cut(s) 69, 215, 411, 529, 949, 1110, 1134
FspBI CTAG 2 cut(s) 743, 846
GluI GCNGC 7 cut(s) 69, 215, 411, 529, 949, 1110, 1134
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 5 cut(s) 92, 155, 238, 720, 844
HapII CCGG 8 cut(s) 179, 234, 273, 301, 570, 675, 717, 935
HgaI GACGC 1 cut(s) 607
Hin1II CATG 9 cut(s) 52, 153, 725, 884, 1007, 1062, 1125, 1133, 1151
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 159, 1116
HinfI GANTC 5 cut(s) 125, 625, 726, 739, 1102
HpaII CCGG 8 cut(s) 179, 234, 273, 301, 570, 675, 717, 935
HphI GGTGA 2 cut(s) 250, 662
Hpy166II GTNNAC 2 cut(s) 129, 713
Hpy188I TCNGA 3 cut(s) 295, 685, 909
Hpy188III TCNNGA 5 cut(s) 49, 374, 604, 622, 966
Hpy8I GTNNAC 2 cut(s) 129, 713
HpyAV CCTTC 3 cut(s) 148, 271, 1072
HpyCH4III ACNGT 3 cut(s) 659, 784, 1156
HpyCH4IV ACGT 2 cut(s) 192, 600
HpyCH4V TGCA 9 cut(s) 149, 217, 243, 405, 547, 772, 1028, 1133, 1159
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 74, 179, 273, 407, 423, 1115
HpyF3I CTNAG 4 cut(s) 58, 64, 123, 861
HpySE526I ACGT 2 cut(s) 192, 600
Hsp92II CATG 9 cut(s) 52, 153, 725, 884, 1007, 1062, 1125, 1133, 1151
KroI GCCGGC 2 cut(s) 178, 272
KroNI GCCGGC 2 cut(s) 180, 274
Kzo9I GATC 4 cut(s) 639, 693, 825, 919
LmnI GCTCC 2 cut(s) 175, 434
Lsp1109I GCAGC 6 cut(s) 201, 422, 515, 935, 1121, 1145
MaeI CTAG 2 cut(s) 743, 846
MaeII ACGT 2 cut(s) 192, 600
MaeIII GTNAC 5 cut(s) 352, 619, 650, 778, 985
MalI GATC 4 cut(s) 641, 695, 827, 921
MboI GATC 4 cut(s) 639, 693, 825, 919
MboII GAAGA 4 cut(s) 37, 130, 649, 770
MhlI GDGCHC 1 cut(s) 581
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 2 cut(s) 369, 925
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 2 cut(s) 134, 748
MmeI TCCRAC 2 cut(s) 486, 901
Mox20I TGGCCA 1 cut(s) 92
MroNI GCCGGC 2 cut(s) 178, 272
MscI TGGCCA 1 cut(s) 92
MseI TTAA 6 cut(s) 54, 555, 705, 786, 972, 1032
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 2 cut(s) 1112, 1136
MspI CCGG 8 cut(s) 179, 234, 273, 301, 570, 675, 717, 935
MspR9I CCNGG 7 cut(s) 235, 302, 571, 675, 717, 935, 954
Mva1269I GAATGC 1 cut(s) 594
MvaI CCWGG 1 cut(s) 954
MwoI GCNNNNNNNGC 7 cut(s) 68, 74, 179, 273, 407, 423, 1115
NaeI GCCGGC 2 cut(s) 180, 274
NciI CCSGG 6 cut(s) 235, 302, 571, 675, 717, 935
NcoI CCATGG 2 cut(s) 721, 1003
NdeII GATC 4 cut(s) 639, 693, 825, 919
NgoMIV GCCGGC 2 cut(s) 178, 272
NlaIII CATG 9 cut(s) 52, 153, 725, 884, 1007, 1062, 1125, 1133, 1151
NlaIV GGNNCC 4 cut(s) 177, 388, 673, 715
NmeAIII GCCGAG 2 cut(s) 149, 222
NmuCI GTSAC 3 cut(s) 619, 650, 985
NspI RCATGY 1 cut(s) 884
PagI TCATGA 1 cut(s) 48
PceI AGGCCT 1 cut(s) 844
PctI GAATGC 1 cut(s) 594
PdiI GCCGGC 2 cut(s) 180, 274
PfeI GAWTC 3 cut(s) 625, 726, 1102
PflFI GACNNNGTC 2 cut(s) 124, 659
PkrI GCNGC 7 cut(s) 70, 216, 412, 530, 950, 1111, 1135
PleI GAGTC 2 cut(s) 133, 747
PpsI GAGTC 2 cut(s) 133, 747
Ppu21I YACGTR 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 952
PspGI CCWGG 1 cut(s) 952
PspN4I GGNNCC 4 cut(s) 177, 388, 673, 715
PspPI GGNCC 5 cut(s) 153, 236, 671, 713, 751
PsyI GACNNNGTC 2 cut(s) 124, 659
PvuII CAGCTG 2 cut(s) 1112, 1136
RsaI GTAC 3 cut(s) 679, 1009, 1055
RsaNI GTAC 3 cut(s) 678, 1008, 1054
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 6 cut(s) 54, 555, 705, 786, 972, 1032
SatI GCNGC 7 cut(s) 69, 215, 411, 529, 949, 1110, 1134
Sau3AI GATC 4 cut(s) 639, 693, 825, 919
Sau96I GGNCC 5 cut(s) 153, 236, 671, 713, 751
SchI GAGTC 2 cut(s) 134, 748
ScrFI CCNGG 7 cut(s) 235, 302, 571, 675, 717, 935, 954
SduI GDGCHC 1 cut(s) 581
SinI GGWCC 3 cut(s) 671, 713, 751
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 2 cut(s) 369, 925
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 3 cut(s) 69, 173, 265
SspMI CTAG 2 cut(s) 743, 846
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 7 cut(s) 233, 300, 569, 673, 715, 933, 952
StyI CCWWGG 2 cut(s) 721, 1003
TaaI ACNGT 3 cut(s) 659, 784, 1156
TaiI ACGT 2 cut(s) 195, 603
TaqI TCGA 5 cut(s) 635, 696, 791, 918, 1038
TaqII GACCGA 1 cut(s) 659
TasI AATT 2 cut(s) 369, 925
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 3 cut(s) 625, 726, 1102
Tru1I TTAA 6 cut(s) 54, 555, 705, 786, 972, 1032
Tru9I TTAA 6 cut(s) 54, 555, 705, 786, 972, 1032
TscAI CASTG 3 cut(s) 436, 592, 856
TseFI GTSAC 3 cut(s) 619, 650, 985
TseI GCWGC 6 cut(s) 214, 410, 528, 948, 1109, 1133
Tsp45I GTSAC 3 cut(s) 619, 650, 985
TspDTI ATGAA 4 cut(s) 37, 130, 1091, 1110
TspRI CASTG 3 cut(s) 436, 592, 856
Tth111I GACNNNGTC 2 cut(s) 124, 659
VpaK11BI GGWCC 3 cut(s) 671, 713, 751
XapI RAATTY 1 cut(s) 369
XceI RCATGY 1 cut(s) 884
XcmI CCANNNNNNNNNTGG 2 cut(s) 761, 1001
XspI CTAG 2 cut(s) 743, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.