FvH4_1g21851

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000702
Physical Location & Seq
Reverse (-)
13780596 .. 13782086
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21851.t1

Sequence Viewer

Length: 1104 bp
ATGACTTACAATGTGGCCGATTTAGGAGCCAAACCAGACGGCCAGACCGACTCAACCAAAGCCTTCCTTTCTGCATGGGACAAAGCCTGTGACTCCGTCGATCCGTCTGTCATCTATGTCCCGACGGGAAGGTTCTTGCTTCGTAATGCGGTTTTCGCCGGACCATGCAAGAACATGGCCATCACTTTTCGCATTGCCGGCACACTCGTGGCCCCTTCAGATTACCGGGTCATAGGAAATGCCGGCAACTGGCTTTTCTTTAGGCATGTGAATGGGGTTACCATCTCCGGTGGAATTCTTGACGGACAAGGCACTGGTTTGTGGGATTGTAAGGCCTCCGGCAAGGGTTGCCCCAGCGGAGCAACGACACTGGGATTTTCCAACTCGAACAACATTGTCGTGAGCGGAGTGACATCCCTAAACAGCCAAATGTTCCACATAGTCATCAACGGCTGCAACAATGTGAAATTGCAAGGTGTTAGGGTTTCTGCCTCCAGTAACAGCCCAAACACGGATGGAATCCACGTCGGAAAGTCCTCCGGCGTCATTATTCTCGACTCTAAGATCGCCACCGGTGACGATTGTGTCTCAATTGGTCCTGGAACAACTAATCTATGGATCGAAAACGTTGCATGCGGACCCGGCCATGGAATCAGCATTGGGAGTCTAGGCAAGGACCAGGAGGAGGAGGGAGTGCAAAATGTAACGGTCAAAACTGTTACATTTACAGGTACTCAAAATGGTCTAAGAATTAAATCATGGGGAAGACTTAGTACTGGTTTTGCTAAGAACATTCTATTCCAACATGCTTTCATGGTCAATGTCCGAAATCCAATCGTTATTGATCAAAATTATTGCCCCGGCGAAATAGGGTGTCCTGGTCAGGATTCGGGAGTTCAGATTAGCGATGTAACATATCAGGACATCCACGGGACATCAGCAACAGAAGTTGCGGTGAAATTTGACTGCAGTTCTAAATTCCCTTGTAGCAAGATCACTCTTGATGATGTGAAGCTCAGTTATAAGAACGAAGCAGCGGAAGCTTCATGTAGCCATGCCGGGGGAACAGCTTTGGGTACTGTTCAGCCTACAAGTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

368

Amino Acids

38.38

Weight (kDa)

6.37

Isoelectric Point (pI)

29.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 4 - 255 1e-13 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 30 - 351 5e-88 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AasI GACNNNNNNGTC 1 cut(s) 584
AccBSI CCGCTC 1 cut(s) 405
AciI CCGC 6 cut(s) 149, 357, 405, 636, 953, 1037
AclI AACGTT 1 cut(s) 627
AclWI GGATC 2 cut(s) 95, 626
AcoI YGGCCR 4 cut(s) 15, 40, 177, 643
AcsI RAATTY 3 cut(s) 294, 959, 977
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 543
AfaI GTAC 3 cut(s) 733, 775, 1078
AfiI CCNNNNNNNGG 5 cut(s) 249, 511, 647, 685, 1060
AgeI ACCGGT 1 cut(s) 572
AjiI CACGTC 1 cut(s) 526
AjnI CCWGG 3 cut(s) 598, 678, 877
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 3 cut(s) 1015, 1043, 1070
AluI AGCT 3 cut(s) 1015, 1043, 1070
Alw26I GTCTC 1 cut(s) 592
AlwI GGATC 2 cut(s) 95, 626
AoxI GGCC 6 cut(s) 15, 40, 177, 210, 333, 643
ApeKI GCWGC 2 cut(s) 453, 1034
ApoI RAATTY 3 cut(s) 294, 959, 977
AsiGI ACCGGT 1 cut(s) 572
AspS9I GGNCC 5 cut(s) 161, 211, 596, 638, 676
AsuC2I CCSGG 4 cut(s) 227, 642, 861, 1060
AsuHPI GGTGA 2 cut(s) 587, 967
AvaII GGWCC 4 cut(s) 161, 596, 638, 676
BalI TGGCCA 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 757, 789
BauI CACGAG 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 772
BbvI GCAGC 2 cut(s) 440, 1046
BccI CCATC 3 cut(s) 188, 290, 509
BceAI ACGGC 2 cut(s) 55, 466
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 3 cut(s) 600, 680, 879
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 4 cut(s) 227, 642, 861, 1060
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 1 cut(s) 967
BisI GCNGC 2 cut(s) 454, 1035
BlsI GCNGC 2 cut(s) 455, 1036
BmcAI AGTACT 1 cut(s) 775
Bme1390I CCNGG 7 cut(s) 227, 600, 642, 680, 861, 879, 1060
Bme18I GGWCC 4 cut(s) 161, 596, 638, 676
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 5 cut(s) 161, 211, 596, 638, 676
BmiI GGNNCC 3 cut(s) 28, 213, 640
BmrFI CCNGG 7 cut(s) 227, 600, 642, 680, 861, 879, 1060
BmrI ACTGGG 1 cut(s) 380
BmuI ACTGGG 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 772
BpmI CTGGAG 1 cut(s) 478
BpuMI CCSGG 4 cut(s) 227, 642, 861, 1060
BsaHI GRCGYC 1 cut(s) 543
BsaJI CCNNGG 4 cut(s) 646, 859, 928, 1059
BsaWI WCCGGW 2 cut(s) 287, 572
Bsc4I CCNNNNNNNGG 5 cut(s) 249, 511, 647, 685, 1060
Bse118I RCCGGY 3 cut(s) 197, 242, 572
Bse1I ACTGG 5 cut(s) 254, 319, 375, 495, 781
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 3 cut(s) 600, 680, 879
BseDI CCNNGG 4 cut(s) 646, 859, 928, 1059
BseGI GGATG 3 cut(s) 413, 520, 924
BseLI CCNNNNNNNGG 5 cut(s) 249, 511, 647, 685, 1060
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 1030
BseNI ACTGG 5 cut(s) 254, 319, 375, 495, 781
BseRI GAGGAG 2 cut(s) 698, 701
BseXI GCAGC 2 cut(s) 440, 1046
BseYI CCCAGC 1 cut(s) 353
BshFI GGCC 6 cut(s) 17, 42, 179, 212, 335, 645
BshTI ACCGGT 1 cut(s) 572
BslFI GGGAC 3 cut(s) 92, 104, 946
BslI CCNNNNNNNGG 5 cut(s) 249, 511, 647, 685, 1060
BsmAI GTCTC 1 cut(s) 592
BsmFI GGGAC 3 cut(s) 92, 104, 946
BsnI GGCC 6 cut(s) 17, 42, 179, 212, 335, 645
Bsp143I GATC 5 cut(s) 100, 564, 618, 844, 993
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 6 cut(s) 149, 357, 405, 636, 953, 1037
BspANI GGCC 6 cut(s) 17, 42, 179, 212, 335, 645
BspCNI CTCAG 1 cut(s) 1029
BspLI GGNNCC 3 cut(s) 28, 213, 640
BspMAI CTGCAG 1 cut(s) 971
BspPI GGATC 2 cut(s) 95, 626
BsrBI CCGCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 3 cut(s) 197, 242, 572
BsrI ACTGG 5 cut(s) 254, 319, 375, 495, 781
BssAI RCCGGY 3 cut(s) 197, 242, 572
BssECI CCNNGG 4 cut(s) 646, 859, 928, 1059
BssMI GATC 5 cut(s) 100, 564, 618, 844, 993
BssNI GRCGYC 1 cut(s) 543
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 646
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 3 cut(s) 600, 680, 879
Bst4CI ACNGT 3 cut(s) 709, 718, 1081
BstACI GRCGYC 1 cut(s) 543
BstAPI GCANNNNNTGC 1 cut(s) 348
BstC8I GCNNGC 3 cut(s) 199, 244, 634
BstDEI CTNAG 5 cut(s) 561, 746, 770, 786, 1016
BstDSI CCRYGG 2 cut(s) 646, 928
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 3 cut(s) 413, 520, 924
BstKTI GATC 5 cut(s) 103, 567, 621, 847, 996
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 5 cut(s) 100, 564, 618, 844, 993
BstMWI GCNNNNNNNGC 5 cut(s) 155, 198, 348, 642, 1040
BstNI CCWGG 3 cut(s) 600, 680, 879
BstNSI RCATGY 3 cut(s) 269, 636, 809
BstPI GGTNACC 1 cut(s) 277
BstSCI CCNGG 7 cut(s) 225, 598, 640, 678, 859, 877, 1058
BstSFI CTRYAG 1 cut(s) 967
BstV1I GCAGC 2 cut(s) 440, 1046
BstV2I GAAGAC 1 cut(s) 772
BsuRI GGCC 6 cut(s) 17, 42, 179, 212, 335, 645
BtgI CCRYGG 2 cut(s) 646, 928
BtgZI GCGATG 1 cut(s) 921
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 3 cut(s) 413, 520, 924
BtsIMutI CAGTG 2 cut(s) 312, 368
Cac8I GCNNGC 3 cut(s) 199, 244, 634
Cfr10I RCCGGY 3 cut(s) 197, 242, 572
Cfr13I GGNCC 5 cut(s) 161, 211, 596, 638, 676
CseI GACGC 1 cut(s) 532
Csp6I GTAC 3 cut(s) 732, 774, 1077
CspAI ACCGGT 1 cut(s) 572
CviQI GTAC 3 cut(s) 732, 774, 1077
DdeI CTNAG 5 cut(s) 561, 746, 770, 786, 1016
DpnI GATC 5 cut(s) 102, 566, 620, 846, 995
DpnII GATC 5 cut(s) 100, 564, 618, 844, 993
DrdI GACNNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 584
EaeI YGGCCR 4 cut(s) 15, 40, 177, 643
Eco130I CCWWGG 1 cut(s) 646
Eco147I AGGCCT 1 cut(s) 335
Eco47I GGWCC 4 cut(s) 161, 596, 638, 676
Eco57I CTGAAG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 277
EcoO65I GGTNACC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 3 cut(s) 598, 678, 877
EcoT14I CCWWGG 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 646
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 3 cut(s) 92, 104, 946
FbaI TGATCA 1 cut(s) 844
Fnu4HI GCNGC 2 cut(s) 454, 1035
FokI GGATG 3 cut(s) 400, 527, 911
Fsp4HI GCNGC 2 cut(s) 454, 1035
FspBI CTAG 1 cut(s) 668
GluI GCNGC 2 cut(s) 454, 1035
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 478
HaeIII GGCC 6 cut(s) 17, 42, 179, 212, 335, 645
HgaI GACGC 1 cut(s) 532
Hin1I GRCGYC 1 cut(s) 543
HindIII AAGCTT 1 cut(s) 1041
HinfI GANTC 7 cut(s) 50, 92, 519, 557, 651, 664, 887
HphI GGTGA 2 cut(s) 587, 967
Hpy188I TCNGA 4 cut(s) 220, 530, 827, 900
Hpy188III TCNNGA 8 cut(s) 121, 299, 400, 554, 884, 891, 920, 1001
Hpy99I CGWCG 3 cut(s) 101, 127, 530
HpyAV CCTTC 3 cut(s) 73, 123, 225
HpyCH4III ACNGT 3 cut(s) 709, 718, 1081
HpyCH4IV ACGT 2 cut(s) 525, 627
HpyCH4V TGCA 7 cut(s) 74, 168, 456, 472, 632, 697, 969
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 198, 348, 642, 1040
HpyF3I CTNAG 5 cut(s) 561, 746, 770, 786, 1016
HpySE526I ACGT 2 cut(s) 525, 627
Hsp92I GRCGYC 1 cut(s) 543
KroI GCCGGC 2 cut(s) 197, 242
KroNI GCCGGC 2 cut(s) 199, 244
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 5 cut(s) 100, 564, 618, 844, 993
LmnI GCTCC 2 cut(s) 26, 359
Lsp1109I GCAGC 2 cut(s) 440, 1046
MaeI CTAG 1 cut(s) 668
MaeII ACGT 2 cut(s) 525, 627
MaeIII GTNAC 8 cut(s) 89, 277, 409, 497, 575, 703, 718, 910
MalI GATC 5 cut(s) 102, 566, 620, 846, 995
MbiI CCGCTC 1 cut(s) 405
MboI GATC 5 cut(s) 100, 564, 618, 844, 993
MboII GAAGA 1 cut(s) 777
MfeI CAATTG 1 cut(s) 591
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 7 cut(s) 294, 467, 591, 750, 850, 959, 977
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 4 cut(s) 44, 86, 551, 673
MmeI TCCRAC 3 cut(s) 405, 508, 826
MnlI CCTC 6 cut(s) 346, 502, 547, 676, 679, 682
Mox20I TGGCCA 1 cut(s) 179
MroNI GCCGGC 2 cut(s) 197, 242
MscI TGGCCA 1 cut(s) 179
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 3 cut(s) 206, 270, 398
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 2 cut(s) 357, 1037
MspR9I CCNGG 7 cut(s) 227, 600, 642, 680, 861, 879, 1060
MunI CAATTG 1 cut(s) 591
MvaI CCWGG 3 cut(s) 600, 680, 879
MwoI GCNNNNNNNGC 5 cut(s) 155, 198, 348, 642, 1040
NaeI GCCGGC 2 cut(s) 199, 244
NciI CCSGG 4 cut(s) 227, 642, 861, 1060
NcoI CCATGG 1 cut(s) 646
NdeII GATC 5 cut(s) 100, 564, 618, 844, 993
NgoMIV GCCGGC 2 cut(s) 197, 242
NlaIV GGNNCC 3 cut(s) 28, 213, 640
NmuCI GTSAC 3 cut(s) 89, 409, 575
NspI RCATGY 3 cut(s) 269, 636, 809
OliI CACNNNNGTG 1 cut(s) 206
PaeI GCATGC 1 cut(s) 636
PceI AGGCCT 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PdiI GCCGGC 2 cut(s) 199, 244
PfeI GAWTC 3 cut(s) 519, 651, 887
PflFI GACNNNGTC 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 598
PinAI ACCGGT 1 cut(s) 572
PkrI GCNGC 2 cut(s) 455, 1036
PleI GAGTC 4 cut(s) 44, 86, 551, 672
PpsI GAGTC 4 cut(s) 44, 86, 551, 672
PsiI TTATAA 1 cut(s) 1023
Psp1406I AACGTT 1 cut(s) 627
Psp6I CCWGG 3 cut(s) 598, 678, 877
PspEI GGTNACC 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 3 cut(s) 598, 678, 877
PspN4I GGNNCC 3 cut(s) 28, 213, 640
PspPI GGNCC 5 cut(s) 161, 211, 596, 638, 676
PstI CTGCAG 1 cut(s) 971
PsyI GACNNNGTC 1 cut(s) 95
RsaI GTAC 3 cut(s) 733, 775, 1078
RsaNI GTAC 3 cut(s) 732, 774, 1077
RseI CAYNNNNRTG 3 cut(s) 206, 270, 398
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 2 cut(s) 454, 1035
Sau3AI GATC 5 cut(s) 100, 564, 618, 844, 993
Sau96I GGNCC 5 cut(s) 161, 211, 596, 638, 676
ScaI AGTACT 1 cut(s) 775
SchI GAGTC 4 cut(s) 44, 86, 551, 673
ScrFI CCNGG 7 cut(s) 227, 600, 642, 680, 861, 879, 1060
SetI ASST 8 cut(s) 134, 478, 528, 630, 733, 1017, 1045, 1072
SfcI CTRYAG 1 cut(s) 967
SgrAI CRCCGGYG 1 cut(s) 572
SinI GGWCC 4 cut(s) 161, 596, 638, 676
SmiMI CAYNNNNRTG 3 cut(s) 206, 270, 398
SphI GCATGC 1 cut(s) 636
Sse9I AATT 7 cut(s) 294, 467, 591, 750, 850, 959, 977
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 6 cut(s) 149, 357, 405, 636, 953, 1037
SspMI CTAG 1 cut(s) 668
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 7 cut(s) 225, 598, 640, 678, 859, 877, 1058
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 3 cut(s) 709, 718, 1081
TaiI ACGT 2 cut(s) 528, 630
TaqI TCGA 4 cut(s) 99, 386, 555, 621
TaqII GACCGA 1 cut(s) 62
TasI AATT 7 cut(s) 294, 467, 591, 750, 850, 959, 977
TatI WGTACW 1 cut(s) 773
TfiI GAWTC 3 cut(s) 519, 651, 887
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 2 cut(s) 319, 375
TseFI GTSAC 3 cut(s) 89, 409, 575
TseI GCWGC 2 cut(s) 453, 1034
Tsp45I GTSAC 3 cut(s) 89, 409, 575
TspDTI ATGAA 2 cut(s) 802, 1035
TspGWI ACGGA 4 cut(s) 85, 93, 318, 527
TspRI CASTG 2 cut(s) 319, 375
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 4 cut(s) 161, 596, 638, 676
XapI RAATTY 3 cut(s) 294, 959, 977
XceI RCATGY 3 cut(s) 269, 636, 809
XspI CTAG 1 cut(s) 668
ZrmI AGTACT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.