Rorug02G0199800

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000702
Physical Location & Seq
Forward (+)
18656625 .. 18660151
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0199800.1

Sequence Viewer

Length: 774 bp
ATGGCCCTAATCTCCACACAAACCCTAACCCTGAAATCCCCTCTTAACCTTTCTCTTCCTTCTCAGCCATCTTCGCAATCTTTCTCTCTCTCACCATCCACTCCTCAGTACTCTCTCCGCACCCCAAAATCAACAGCACGGTTTAGCGCTAGACGACTCGTCGTTTCCAGAGCCACCACATCCTCTGCTTTTGATTTCAGCCCCAGCATCGGTGAGGTCCTCGGTGAAGTTGGTATCTTCACCGCTGCTGGTGATCCCGTCCGGTTCAACGATCTATTGGATCAAAACGAGGGGATAGTTGTTGTTGCGCTATTGAGGCACTTTGGATGCGTTTGCTGTTGGGAACTTGCTTCAGCTCTAAAAGAATCAAAAGCTAGATTTGACTCAGCTGGTGTGAAACTAATCGCGGTCGGTGTTGGCACTCCTGATAAAGCTCGCATCCTTGCAGAACGGTTACCATTTCCCATGGATTCCCTTTATGCCGATCCTGATCGTAAGGCATATGATGTTTTGGGCTTATACTTTGGATTGGGTCGAACATTCTTCAATCCAGCTAGTGCAAAGGTGTTCTCAAGAATTGAGGCCCTGCAGAAAGCTTTAAAGAACTATACGATTAAAGCCACTCCAGATGATATAAATAGTGTGTTACAACAGGGTGGGATGTTCGTCTTCAAAGGGAAGCAGTTATTGTATGCTCGGAAAGACGAAGGGACAGGTGATCATGCCCCATTAGATGATATCTTTGATGTTTGTTGCAAAGTTCCTGTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

257

Amino Acids

27.86

Weight (kDa)

8.77

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AhpC-TSA_2 PF13911 120 - 236 4.3e-19 AhpC/TSA antioxidant enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 407
AciI CCGC 3 cut(s) 118, 243, 407
AclWI GGATC 3 cut(s) 248, 288, 479
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 1 cut(s) 110
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 3 cut(s) 268, 547, 673
AhdI GACNNNNNGTC 1 cut(s) 158
AluBI AGCT 6 cut(s) 356, 374, 389, 434, 554, 596
AluI AGCT 6 cut(s) 356, 374, 389, 434, 554, 596
AlwI GGATC 3 cut(s) 248, 288, 479
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 2 cut(s) 3, 582
ApeKI GCWGC 1 cut(s) 245
ArsI GACNNNNNNTTYG 1 cut(s) 750
AspLEI GCGC 2 cut(s) 149, 310
AspS9I GGNCC 3 cut(s) 4, 217, 583
AsuHPI GGTGA 6 cut(s) 84, 224, 232, 236, 263, 728
AvaII GGWCC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 661
BbvI GCAGC 1 cut(s) 232
BccI CCATC 2 cut(s) 76, 103
BclI TGATCA 1 cut(s) 718
BfaI CTAG 3 cut(s) 150, 375, 555
BfmI CTRYAG 1 cut(s) 587
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
BmcAI AGTACT 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 217
BmeRI GACNNNNNGTC 1 cut(s) 158
BmgT120I GGNCC 3 cut(s) 4, 217, 583
BmsI GCATC 3 cut(s) 216, 317, 447
BpiI GAAGAC 1 cut(s) 661
BpmI CTGGAG 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 556
BsaBI GATNNNNATC 1 cut(s) 489
BsaJI CCNNGG 2 cut(s) 220, 465
BsaWI WCCGGW 1 cut(s) 261
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse8I GATNNNNATC 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 220, 465
BseGI GGATG 5 cut(s) 95, 179, 332, 438, 666
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMII CTCAG 3 cut(s) 77, 119, 399
BseRI GAGGAG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 407
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 2 cut(s) 5, 584
BsiEI CGRYCG 1 cut(s) 411
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 724
BsnI GGCC 2 cut(s) 5, 584
Bsp143I GATC 6 cut(s) 253, 271, 280, 484, 490, 718
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 3 cut(s) 118, 243, 407
BspANI GGCC 2 cut(s) 5, 584
BspCNI CTCAG 3 cut(s) 76, 118, 398
BspFNI CGCG 1 cut(s) 407
BspMAI CTGCAG 1 cut(s) 591
BspPI GGATC 3 cut(s) 248, 288, 479
BssECI CCNNGG 2 cut(s) 220, 465
BssMI GATC 6 cut(s) 253, 271, 280, 484, 490, 718
BssT1I CCWWGG 1 cut(s) 465
Bst4CI ACNGT 2 cut(s) 141, 453
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 3 cut(s) 63, 105, 385
BstDSI CCRYGG 1 cut(s) 465
BstEII GGTNACC 1 cut(s) 453
BstF5I GGATG 5 cut(s) 95, 179, 332, 438, 666
BstFNI CGCG 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 310
BstKTI GATC 6 cut(s) 256, 274, 283, 487, 493, 721
BstMBI GATC 6 cut(s) 253, 271, 280, 484, 490, 718
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 2 cut(s) 73, 316
BstPI GGTNACC 1 cut(s) 453
BstSFI CTRYAG 1 cut(s) 587
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 661
BsuRI GGCC 2 cut(s) 5, 584
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 5 cut(s) 95, 179, 332, 438, 666
Cac8I GCNNGC 1 cut(s) 436
CfoI GCGC 2 cut(s) 149, 310
Cfr13I GGNCC 3 cut(s) 4, 217, 583
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 466, 722
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 3 cut(s) 63, 105, 385
DpnI GATC 6 cut(s) 255, 273, 282, 486, 492, 720
DpnII GATC 6 cut(s) 253, 271, 280, 484, 490, 718
DraI TTTAAA 1 cut(s) 600
DriI GACNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 1 cut(s) 60
Eam1105I GACNNNNNGTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 465
Eco32I GATATC 1 cut(s) 739
Eco47I GGWCC 1 cut(s) 217
Eco47III AGCGCT 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 336
Eco91I GGTNACC 1 cut(s) 453
EcoO109I RGGNCCY 2 cut(s) 217, 583
EcoO65I GGTNACC 1 cut(s) 453
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 469, 725
FaiI YATR 9 cut(s) 467, 480, 502, 504, 520, 609, 635, 693, 723
FaqI GGGAC 1 cut(s) 724
FatI CATG 2 cut(s) 465, 721
FauNDI CATATG 1 cut(s) 502
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 1 cut(s) 246
FokI GGATG 5 cut(s) 82, 166, 339, 425, 673
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 3 cut(s) 150, 375, 555
GlaI GCGC 2 cut(s) 148, 309
GluI GCNGC 1 cut(s) 246
GsaI CCCAGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 5, 584
HapII CCGG 1 cut(s) 262
HhaI GCGC 2 cut(s) 149, 310
Hin1II CATG 2 cut(s) 469, 725
Hin6I GCGC 2 cut(s) 147, 308
HinP1I GCGC 2 cut(s) 147, 308
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 4 cut(s) 156, 365, 383, 470
HpaII CCGG 1 cut(s) 262
HphI GGTGA 6 cut(s) 84, 224, 232, 236, 263, 728
Hpy188I TCNGA 1 cut(s) 699
Hpy188III TCNNGA 6 cut(s) 168, 425, 488, 573, 626, 771
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 69, 701
HpyCH4III ACNGT 2 cut(s) 141, 453
HpyCH4V TGCA 4 cut(s) 446, 560, 589, 756
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 316
HpyF3I CTNAG 3 cut(s) 63, 105, 385
Hsp92II CATG 2 cut(s) 469, 725
HspAI GCGC 2 cut(s) 147, 308
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 6 cut(s) 253, 271, 280, 484, 490, 718
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 3 cut(s) 216, 317, 447
MaeI CTAG 3 cut(s) 150, 375, 555
MaeIII GTNAC 2 cut(s) 453, 645
MalI GATC 6 cut(s) 255, 273, 282, 486, 492, 720
MboI GATC 6 cut(s) 253, 271, 280, 484, 490, 718
MboII GAAGA 5 cut(s) 47, 63, 229, 535, 661
MluCI AATT 1 cut(s) 576
MlyI GAGTC 2 cut(s) 150, 377
MnlI CCTC 8 cut(s) 51, 114, 193, 208, 230, 283, 309, 574
MseI TTAA 3 cut(s) 45, 599, 615
MspA1I CMGCKG 2 cut(s) 245, 389
MspI CCGG 1 cut(s) 262
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 73, 316
NcoI CCATGG 1 cut(s) 465
NdeI CATATG 1 cut(s) 502
NdeII GATC 6 cut(s) 253, 271, 280, 484, 490, 718
NlaIII CATG 2 cut(s) 469, 725
PfeI GAWTC 2 cut(s) 365, 470
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 2 cut(s) 150, 377
PpsI GAGTC 2 cut(s) 150, 377
PpuMI RGGWCCY 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 217
PspEI GGTNACC 1 cut(s) 453
PspFI CCCAGC 1 cut(s) 203
PspPI GGNCC 3 cut(s) 4, 217, 583
PspPPI RGGWCCY 1 cut(s) 217
PstI CTGCAG 1 cut(s) 591
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 3 cut(s) 45, 599, 615
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 6 cut(s) 253, 271, 280, 484, 490, 718
Sau96I GGNCC 3 cut(s) 4, 217, 583
ScaI AGTACT 1 cut(s) 110
SchI GAGTC 2 cut(s) 150, 377
SfaNI GCATC 3 cut(s) 216, 317, 447
SfcI CTRYAG 1 cut(s) 587
SinI GGWCC 1 cut(s) 217
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
Sse9I AATT 1 cut(s) 576
SsiI CCGC 3 cut(s) 118, 243, 407
SspMI CTAG 3 cut(s) 150, 375, 555
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 2 cut(s) 141, 453
TaqI TCGA 1 cut(s) 535
TasI AATT 1 cut(s) 576
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 2 cut(s) 365, 470
Tru1I TTAA 3 cut(s) 45, 599, 615
Tru9I TTAA 3 cut(s) 45, 599, 615
TseI GCWGC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 217
XspI CTAG 3 cut(s) 150, 375, 555
ZrmI AGTACT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.