Prupe.4G261800_v2.0.a1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000702
Physical Location & Seq
Forward (+)
19028015 .. 19029785
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G261800.1

Sequence Viewer

Length: 1179 bp
ATGGCAAACCCTAGAAGCCTAATTGTTCTCTCACTAGCCTTTGTGTTCATGATCAACTCAGCCTTGGCCACTGCGGTTACATACAATGTGGCCAGCTTAGGAGCCAAAGCAAATGGCAAGACTGACTCCACCAAAGCCTTCCTCTCTGCATGGGCCAAAGCTTGTGCCTCTGTGAAGCCCGTTGTCATTTACGTTCCGGCAGGGAGGTTCTTACTTCGCAATGTGGTATTCAGCGGGCCATGCAAGAACAACGCCATCACCTTCCGCATTGCTGGAACCCTCGTGGCCCCGTCCAATTACGGGGTCATTGGAAATGCCGATAACTGGATTTTCTTTCAACATGTTAACGGGGTTACAATATCGGGTGGAATTCTTGACGGCCAAGGCACCGCCTTGTGGGCTTGCAAGGCTTCGGGCAAGAGTTGTCCCAGCGGAGCAACGACATTGGGTTTTTCCAACTCCAACAACATTAAGGTGAGTGGCTTAATATCCCTCAACAGCCAAATGTTCCACATAGTCGTCAACGGCTGCCACAATGTGAAAATGGATGGTGTTAGGGTTTCGGCGTCCAGCAATAGCCCCAACACCGACGGGATTCACGTTCAAATGTCATCCGGTGTTACCATCCTCAACTCCAAGATTGCAACTGGTGATGATTGTGTCTCAATTGGCCCCGGAACCTCAAATTTGTGGATCGAAAATGTTGCATGTGGACCCGGTCATGGAATAAGCATTGGGAGTCTAGGCAAGGATCAACAAGAGGCTGGTGTGCAAAATGTAACAGTTAAAACGGTTACTTTTACTGGTACCCAAAACGGTTTAAGGATCAAGTCTTGGGGGAGGCCGAGCACTGGGTTTGCTAGGAACATTCTTTTTCAACATGCCGTAATGATCAATGTCCAAAATCCCATTGTTATCGACCAAAACTATTGCCCTGAAAAAAAAGGGTGTCCTGGTCAGGTTTCTGGAGTTCGAATTAGCGATGTGACATACCAAGACATCCACGGTTCATCGGCAACAGAAGTTGCAGTTAAATTTGACTGTAGTTCCAAGTACCCTTGCAGCAAGATCAGATTGGAGAATGTGAAACTTACTTATAAGAACCAAGTTGCTGTAGCTACATGTAGCCATGCAGGTGGAACAGCTGCGGGCATGGTTCAACCTACAAGTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

393

Amino Acids

40.98

Weight (kDa)

9.28

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
AarI CACCTGC 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1124
Acc65I GGTACC 1 cut(s) 806
AccB1I GGYRCC 2 cut(s) 386, 806
AciI CCGC 6 cut(s) 74, 234, 265, 390, 432, 1148
AclWI GGATC 3 cut(s) 701, 759, 833
AcoI YGGCCR 3 cut(s) 66, 90, 379
AcsI RAATTY 3 cut(s) 369, 685, 1034
AcyI GRCGYC 1 cut(s) 566
AdeI CACNNNGTG 1 cut(s) 538
AfaI GTAC 2 cut(s) 808, 1055
AfiI CCNNNNNNNGG 5 cut(s) 300, 324, 396, 722, 851
AflIII ACRYGT 2 cut(s) 340, 1121
AgsI TTSAA 4 cut(s) 338, 605, 878, 1160
AjnI CCWGG 1 cut(s) 952
AluBI AGCT 4 cut(s) 96, 161, 1118, 1145
AluI AGCT 4 cut(s) 96, 161, 1118, 1145
Alw21I GWGCWC 1 cut(s) 851
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 3 cut(s) 701, 759, 833
AoxI GGCC 8 cut(s) 66, 90, 153, 236, 285, 379, 670, 842
ApeKI GCWGC 3 cut(s) 528, 1062, 1145
ApoI RAATTY 3 cut(s) 369, 685, 1034
Asp718I GGTACC 1 cut(s) 806
AspS9I GGNCC 5 cut(s) 153, 236, 286, 671, 713
AsuC2I CCSGG 2 cut(s) 675, 717
AsuHPI GGTGA 3 cut(s) 250, 487, 662
AsuII TTCGAA 1 cut(s) 973
AvaII GGWCC 1 cut(s) 713
BalI TGGCCA 2 cut(s) 68, 92
BanI GGYRCC 2 cut(s) 386, 806
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 851
BbvI GCAGC 3 cut(s) 515, 1074, 1132
BccI CCATC 3 cut(s) 263, 542, 632
BceAI ACGGC 3 cut(s) 394, 541, 869
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 954
BclI TGATCA 2 cut(s) 51, 891
BcnI CCSGG 2 cut(s) 675, 717
BcoDI GTCTC 1 cut(s) 667
BfaI CTAG 4 cut(s) 12, 35, 743, 861
BfmI CTRYAG 2 cut(s) 1042, 1113
BfuAI ACCTGC 1 cut(s) 1124
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 3 cut(s) 529, 1063, 1146
BlsI GCNGC 3 cut(s) 530, 1064, 1147
Bme1390I CCNGG 3 cut(s) 675, 717, 954
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 5 cut(s) 153, 236, 286, 671, 713
BmiI GGNNCC 8 cut(s) 103, 277, 288, 388, 673, 679, 715, 808
BmrFI CCNGG 3 cut(s) 675, 717, 954
BmrI ACTGGG 1 cut(s) 861
BmuI ACTGGG 1 cut(s) 861
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 1 cut(s) 97
Bpu14I TTCGAA 1 cut(s) 973
BpuMI CCSGG 2 cut(s) 675, 717
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 4 cut(s) 63, 382, 673, 1003
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 5 cut(s) 300, 324, 396, 722, 851
Bse1I ACTGG 4 cut(s) 329, 652, 808, 856
Bse3DI GCAATG 2 cut(s) 226, 267
BseBI CCWGG 1 cut(s) 954
BseDI CCNNGG 4 cut(s) 63, 382, 673, 1003
BseGI GGATG 4 cut(s) 553, 611, 624, 999
BseLI CCNNNNNNNGG 5 cut(s) 300, 324, 396, 722, 851
BseMI GCAATG 2 cut(s) 226, 267
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 4 cut(s) 329, 652, 808, 856
BseXI GCAGC 3 cut(s) 515, 1074, 1132
BseYI CCCAGC 1 cut(s) 428
BshFI GGCC 8 cut(s) 68, 92, 155, 238, 287, 381, 672, 844
BshNI GGYRCC 2 cut(s) 386, 806
BsiHKAI GWGCWC 1 cut(s) 851
BsiSI CCGG 4 cut(s) 197, 615, 675, 717
BslFI GGGAC 1 cut(s) 411
BslI CCNNNNNNNGG 5 cut(s) 300, 324, 396, 722, 851
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 411
BsnI GGCC 8 cut(s) 68, 92, 155, 238, 287, 381, 672, 844
Bsp119I TTCGAA 1 cut(s) 973
Bsp1286I GDGCHC 1 cut(s) 851
Bsp143I GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
BspACI CCGC 6 cut(s) 74, 234, 265, 390, 432, 1148
BspANI GGCC 8 cut(s) 68, 92, 155, 238, 287, 381, 672, 844
BspCNI CTCAG 1 cut(s) 71
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 8 cut(s) 103, 277, 288, 388, 673, 679, 715, 808
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 3 cut(s) 701, 759, 833
BspT104I TTCGAA 1 cut(s) 973
BspT107I GGYRCC 2 cut(s) 386, 806
BsrDI GCAATG 2 cut(s) 226, 267
BsrI ACTGG 4 cut(s) 329, 652, 808, 856
BssECI CCNNGG 4 cut(s) 63, 382, 673, 1003
BssMI GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
BssNI GRCGYC 1 cut(s) 566
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 63, 382
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 954
Bst4CI ACNGT 5 cut(s) 784, 793, 818, 1007, 1043
BstACI GRCGYC 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 973
BstC8I GCNNGC 4 cut(s) 94, 236, 403, 1150
BstDEI CTNAG 2 cut(s) 58, 97
BstDSI CCRYGG 1 cut(s) 1003
BstF5I GGATG 4 cut(s) 553, 611, 624, 999
BstKTI GATC 6 cut(s) 54, 696, 754, 828, 894, 1071
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
BstMWI GCNNNNNNNGC 3 cut(s) 240, 398, 407
BstNI CCWGG 1 cut(s) 954
BstNSI RCATGY 4 cut(s) 344, 711, 884, 1125
BstSCI CCNGG 3 cut(s) 673, 715, 952
BstSFI CTRYAG 2 cut(s) 1042, 1113
BstV1I GCAGC 3 cut(s) 515, 1074, 1132
BstXI CCANNNNNNTGG 1 cut(s) 1136
BsuRI GGCC 8 cut(s) 68, 92, 155, 238, 287, 381, 672, 844
BtgI CCRYGG 1 cut(s) 1003
BtgZI GCGATG 1 cut(s) 996
BtsCI GGATG 4 cut(s) 553, 611, 624, 999
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 849
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 4 cut(s) 94, 236, 403, 1150
CciI TCATGA 1 cut(s) 48
Cfr13I GGNCC 5 cut(s) 153, 236, 286, 671, 713
CseI GACGC 1 cut(s) 555
Csp6I GTAC 2 cut(s) 807, 1054
CviQI GTAC 2 cut(s) 807, 1054
DdeI CTNAG 2 cut(s) 58, 97
DpnI GATC 6 cut(s) 53, 695, 753, 827, 893, 1070
DpnII GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
DraIII CACNNNGTG 1 cut(s) 538
EaeI YGGCCR 3 cut(s) 66, 90, 379
Eco130I CCWWGG 2 cut(s) 63, 382
Eco47I GGWCC 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 952
EcoT14I CCWWGG 2 cut(s) 63, 382
ErhI CCWWGG 2 cut(s) 63, 382
FaqI GGGAC 1 cut(s) 411
FauI CCCGC 2 cut(s) 227, 1141
FbaI TGATCA 2 cut(s) 51, 891
Fnu4HI GCNGC 3 cut(s) 529, 1063, 1146
FokI GGATG 4 cut(s) 560, 598, 611, 986
Fsp4HI GCNGC 3 cut(s) 529, 1063, 1146
FspBI CTAG 4 cut(s) 12, 35, 743, 861
GluI GCNGC 3 cut(s) 529, 1063, 1146
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 8 cut(s) 68, 92, 155, 238, 287, 381, 672, 844
HapII CCGG 4 cut(s) 197, 615, 675, 717
HgaI GACGC 1 cut(s) 555
Hin1I GRCGYC 1 cut(s) 566
HincII GTYRAC 2 cut(s) 346, 523
HindII GTYRAC 2 cut(s) 346, 523
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 3 cut(s) 125, 595, 739
HpaI GTTAAC 1 cut(s) 346
HpaII CCGG 4 cut(s) 197, 615, 675, 717
HphI GGTGA 3 cut(s) 250, 487, 662
Hpy166II GTNNAC 3 cut(s) 346, 523, 713
Hpy188I TCNGA 1 cut(s) 1073
Hpy188III TCNNGA 3 cut(s) 49, 374, 966
Hpy8I GTNNAC 3 cut(s) 346, 523, 713
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 2 cut(s) 148, 271
HpyCH4III ACNGT 5 cut(s) 784, 793, 818, 1007, 1043
HpyCH4IV ACGT 2 cut(s) 192, 600
HpyCH4V TGCA 9 cut(s) 149, 243, 405, 644, 707, 772, 1028, 1062, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 398, 407
HpyF3I CTNAG 2 cut(s) 58, 97
HpySE526I ACGT 2 cut(s) 192, 600
Hsp92I GRCGYC 1 cut(s) 566
KpnI GGTACC 1 cut(s) 810
Ksp22I TGATCA 2 cut(s) 51, 891
KspAI GTTAAC 1 cut(s) 346
Kzo9I GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
LmnI GCTCC 2 cut(s) 101, 434
Lsp1109I GCAGC 3 cut(s) 515, 1074, 1132
MaeI CTAG 4 cut(s) 12, 35, 743, 861
MaeII ACGT 2 cut(s) 192, 600
MaeIII GTNAC 6 cut(s) 76, 352, 619, 778, 793, 985
MalI GATC 6 cut(s) 53, 695, 753, 827, 893, 1070
MboI GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
MfeI CAATTG 1 cut(s) 666
MhlI GDGCHC 1 cut(s) 851
MlsI TGGCCA 2 cut(s) 68, 92
MluCI AATT 7 cut(s) 21, 295, 369, 666, 685, 975, 1034
MluNI TGGCCA 2 cut(s) 68, 92
MlyI GAGTC 2 cut(s) 119, 748
MmeI TCCRAC 2 cut(s) 480, 486
MnlI CCTC 9 cut(s) 152, 178, 198, 290, 503, 638, 691, 754, 834
Mox20I TGGCCA 2 cut(s) 68, 92
MscI TGGCCA 2 cut(s) 68, 92
MseI TTAA 6 cut(s) 345, 471, 485, 786, 821, 1032
MslI CAYNNNNRTG 2 cut(s) 473, 1134
Msp20I TGGCCA 2 cut(s) 68, 92
MspA1I CMGCKG 3 cut(s) 234, 432, 1145
MspI CCGG 4 cut(s) 197, 615, 675, 717
MspR9I CCNGG 3 cut(s) 675, 717, 954
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 954
MwoI GCNNNNNNNGC 3 cut(s) 240, 398, 407
NciI CCSGG 2 cut(s) 675, 717
NdeII GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
NlaIV GGNNCC 8 cut(s) 103, 277, 288, 388, 673, 679, 715, 808
NmeAIII GCCGAG 1 cut(s) 870
NmuCI GTSAC 1 cut(s) 985
NspI RCATGY 4 cut(s) 344, 711, 884, 1125
NspV TTCGAA 1 cut(s) 973
PagI TCATGA 1 cut(s) 48
PaqCI CACCTGC 1 cut(s) 1124
PciI ACATGT 2 cut(s) 340, 1121
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PfeI GAWTC 1 cut(s) 595
PflFI GACNNNGTC 1 cut(s) 717
PkrI GCNGC 3 cut(s) 530, 1064, 1147
PleI GAGTC 2 cut(s) 119, 747
PpsI GAGTC 2 cut(s) 119, 747
PscI ACATGT 2 cut(s) 340, 1121
PsiI TTATAA 1 cut(s) 1098
Psp6I CCWGG 1 cut(s) 952
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 1 cut(s) 952
PspN4I GGNNCC 8 cut(s) 103, 277, 288, 388, 673, 679, 715, 808
PspPI GGNCC 5 cut(s) 153, 236, 286, 671, 713
PsyI GACNNNGTC 1 cut(s) 717
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 2 cut(s) 808, 1055
RsaNI GTAC 2 cut(s) 807, 1054
RseI CAYNNNNRTG 2 cut(s) 473, 1134
SaqAI TTAA 6 cut(s) 345, 471, 485, 786, 821, 1032
SatI GCNGC 3 cut(s) 529, 1063, 1146
Sau3AI GATC 6 cut(s) 51, 693, 751, 825, 891, 1068
Sau96I GGNCC 5 cut(s) 153, 236, 286, 671, 713
SchI GAGTC 2 cut(s) 119, 748
ScrFI CCNGG 3 cut(s) 675, 717, 954
SduI GDGCHC 1 cut(s) 851
SfcI CTRYAG 2 cut(s) 1042, 1113
SfuI TTCGAA 1 cut(s) 973
SinI GGWCC 1 cut(s) 713
SmiMI CAYNNNNRTG 2 cut(s) 473, 1134
Sse9I AATT 7 cut(s) 21, 295, 369, 666, 685, 975, 1034
SsiI CCGC 6 cut(s) 74, 234, 265, 390, 432, 1148
SspMI CTAG 4 cut(s) 12, 35, 743, 861
StyD4I CCNGG 3 cut(s) 673, 715, 952
StyI CCWWGG 2 cut(s) 63, 382
TaaI ACNGT 5 cut(s) 784, 793, 818, 1007, 1043
TaiI ACGT 2 cut(s) 195, 603
TaqI TCGA 3 cut(s) 696, 918, 973
TasI AATT 7 cut(s) 21, 295, 369, 666, 685, 975, 1034
TfiI GAWTC 1 cut(s) 595
Tru1I TTAA 6 cut(s) 345, 471, 485, 786, 821, 1032
Tru9I TTAA 6 cut(s) 345, 471, 485, 786, 821, 1032
TscAI CASTG 2 cut(s) 76, 856
TseFI GTSAC 1 cut(s) 985
TseI GCWGC 3 cut(s) 528, 1062, 1145
Tsp45I GTSAC 1 cut(s) 985
TspDTI ATGAA 2 cut(s) 37, 999
TspRI CASTG 2 cut(s) 76, 856
Tth111I GACNNNGTC 1 cut(s) 717
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 3 cut(s) 369, 685, 1034
XceI RCATGY 4 cut(s) 344, 711, 884, 1125
XspI CTAG 4 cut(s) 12, 35, 743, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.