Rw2G020150

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000702
Physical Location & Seq
Reverse (-)
27324795 .. 27326420
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020150.1

Sequence Viewer

Length: 1176 bp
ATGGCGAATCCCAGAAGCCACATTTTTCTCTCACTCCTCTTTTTCTTCATAAACTCCGGTGTCTCCAATGCAGTTACTTACAATGTGGCCGATTTAGGAGCCAAACCAGACGGCCAGACCGACTCAACCAAAGCCTTCCTTTCTGCATGGGCCGAAGCTTGTGCCTCGGCCAATCCCTCGGTTATTTATGTCCCGGCCGGAAGGTTTTTGCTTCGCAATGCAGTCTTCAGCGGACCATGTAAAAACATAGCCATCACATTTCGCATTGCCGGCACACTCGTGTCCCCGTCGGATTACCGAGTCATAGGAAATGCCGGTAACTGGCTTTTGTTTGAGCATGTGAATGGGATTACAATCTCGGGTGGAATTCTTGATGGACAAGGCACTGGTCTGTGGGATTGCAAAGCCAACGGCAATAGTTGCCCCAGCGGAGCAACTACGCTTGGGTTTTCCAACTCCAACAACATTGTCGTGAGCGGATTGACCTCCCTAAACAGCCAAATGTTTCACATAGTCATCAACGGCTGCAACAACGTGAAAATGCAAGGTGTTAGGGTTTCTGCCTCCGGTAACAGCCCAAACACCGACGGAATCCACGTCCAACAGTCCTCGGGCGTCACTGTTCTCGACTCTAAAATTGCAACCGGTGACGACTGTGTCTCAATTGGTCCTGGAACCACTAATTTGTGGATCGAAAACATTGCATGTGGACCAGGCCATGGTATCAGCATTGGAAGTCTAGGCAAGGACCAGCAGGAGGATGGTGTGCAAAATGTAACAGTGAAAACTGTTACATTTACCGGTACTCAAAATGGTGTGAGAATTAAATCATGGGGGAGACCTAGCACGGGGTTTGCCAAGAACATTCTTTTCCAACACGCTGTGATGGTCGATGTCCAAAATCCTATCATCATTGATCAAAATTACTGCCCTGGCGAGAAAGGTTGTCCTGGTCAGGTTTCGGGAGTTCAAATTAGCGATGTGACATATCAAGACATCCACGGGACATCAGCAACAGAAGTTGCGGTGAAATTCGACTGTAGTTCAAAGTATCCTTGTAACAAGATCACATTGAATGATGTGAAGCTCAATTACAAGAACCAAGCGGCCGAAGCTTCATGTAGCCATGCCGGTGGAACGGCTGAGGGTACGGTTCAGCCTACAAGTTGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

391

Amino Acids

40.92

Weight (kDa)

6.43

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 28 - 279 2.7e-14 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 54 - 375 9.7e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 656
AccB7I CCANNNNNTGG 1 cut(s) 719
AccBSI CCGCTC 1 cut(s) 477
AciI CCGC 5 cut(s) 231, 429, 477, 1025, 1106
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 5 cut(s) 87, 112, 168, 195, 1107
AcsI RAATTY 2 cut(s) 366, 1031
AcuI CTGAAG 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 615
AdeI CACNNNGTG 1 cut(s) 883
AfaI GTAC 2 cut(s) 805, 1150
AfiI CCNNNNNNNGG 4 cut(s) 321, 719, 757, 848
AgeI ACCGGT 2 cut(s) 644, 800
AgsI TTSAA 3 cut(s) 971, 1047, 1075
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 4 cut(s) 670, 712, 931, 949
AleI CACNNNNGTG 1 cut(s) 278
AluBI AGCT 3 cut(s) 158, 1087, 1115
AluI AGCT 3 cut(s) 158, 1087, 1115
Alw26I GTCTC 3 cut(s) 67, 664, 832
AlwI GGATC 1 cut(s) 698
Ama87I CYCGRG 2 cut(s) 358, 610
AoxI GGCC 7 cut(s) 87, 112, 150, 168, 195, 715, 1107
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 2 cut(s) 366, 1031
AsiGI ACCGGT 2 cut(s) 644, 800
AspS9I GGNCC 5 cut(s) 150, 233, 668, 710, 748
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 659, 1039
AvaI CYCGRG 2 cut(s) 358, 610
AvaII GGWCC 4 cut(s) 233, 668, 710, 748
BarI GAAGNNNNNNTAC 2 cut(s) 1076, 1108
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 1143
BbvI GCAGC 1 cut(s) 512
BccI CCATC 4 cut(s) 260, 368, 755, 880
BceAI ACGGC 4 cut(s) 127, 427, 538, 1155
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 683, 717
BciT130I CCWGG 4 cut(s) 672, 714, 933, 951
BciVI GTATCC 1 cut(s) 1062
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 3 cut(s) 67, 664, 832
BfaI CTAG 2 cut(s) 740, 843
BfmI CTRYAG 2 cut(s) 1039, 1172
BfuI GTATCC 1 cut(s) 1062
BisI GCNGC 2 cut(s) 526, 1107
BlsI GCNGC 2 cut(s) 527, 1108
Bme1390I CCNGG 5 cut(s) 194, 672, 714, 933, 951
Bme18I GGWCC 4 cut(s) 233, 668, 710, 748
BmeT110I CYCGRG 2 cut(s) 358, 610
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 5 cut(s) 150, 233, 668, 710, 748
BmiI GGNNCC 2 cut(s) 100, 676
BmrFI CCNGG 5 cut(s) 194, 672, 714, 933, 951
BpiI GAAGAC 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 1143
BpuMI CCSGG 1 cut(s) 194
BsaBI GATNNNNATC 1 cut(s) 353
BsaHI GRCGYC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 832
BsaJI CCNNGG 6 cut(s) 165, 177, 609, 718, 931, 1000
BsaWI WCCGGW 4 cut(s) 56, 566, 644, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 321, 719, 757, 848
Bse118I RCCGGY 5 cut(s) 269, 314, 644, 800, 1130
Bse1I ACTGG 2 cut(s) 326, 391
Bse3DI GCAATG 3 cut(s) 223, 264, 699
Bse8I GATNNNNATC 1 cut(s) 353
BseBI CCWGG 4 cut(s) 672, 714, 933, 951
BseDI CCNNGG 6 cut(s) 165, 177, 609, 718, 931, 1000
BseGI GGATG 2 cut(s) 766, 996
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 4 cut(s) 321, 719, 757, 848
BseMI GCAATG 3 cut(s) 223, 264, 699
BseMII CTCAG 1 cut(s) 1134
BseNI ACTGG 2 cut(s) 326, 391
BseRI GAGGAG 1 cut(s) 26
BseX3I CGGCCG 2 cut(s) 195, 1107
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 425
Bsh1285I CGRYCG 2 cut(s) 198, 1110
BshFI GGCC 7 cut(s) 89, 114, 152, 170, 197, 717, 1109
BshTI ACCGGT 2 cut(s) 644, 800
BsiEI CGRYCG 2 cut(s) 198, 1110
BsiHKCI CYCGRG 2 cut(s) 358, 610
BsiSI CCGG 9 cut(s) 57, 194, 198, 270, 315, 567, 645, 801, 1131
BslFI GGGAC 3 cut(s) 176, 268, 1018
BslI CCNNNNNNNGG 4 cut(s) 321, 719, 757, 848
BsmAI GTCTC 3 cut(s) 67, 664, 832
BsmFI GGGAC 3 cut(s) 176, 268, 1018
BsnI GGCC 7 cut(s) 89, 114, 152, 170, 197, 717, 1109
Bso31I GGTCTC 1 cut(s) 832
BsoBI CYCGRG 2 cut(s) 358, 610
Bsp143I GATC 3 cut(s) 690, 916, 1065
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 5 cut(s) 231, 429, 477, 1025, 1106
BspANI GGCC 7 cut(s) 89, 114, 152, 170, 197, 717, 1109
BspCNI CTCAG 1 cut(s) 1135
BspLI GGNNCC 2 cut(s) 100, 676
BspPI GGATC 1 cut(s) 698
BspTNI GGTCTC 1 cut(s) 832
BsrBI CCGCTC 1 cut(s) 477
BsrDI GCAATG 3 cut(s) 223, 264, 699
BsrFI RCCGGY 5 cut(s) 269, 314, 644, 800, 1130
BsrI ACTGG 2 cut(s) 326, 391
BssAI RCCGGY 5 cut(s) 269, 314, 644, 800, 1130
BssECI CCNNGG 6 cut(s) 165, 177, 609, 718, 931, 1000
BssMI GATC 3 cut(s) 690, 916, 1065
BssNI GRCGYC 1 cut(s) 615
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 718
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 4 cut(s) 672, 714, 933, 951
Bst4CI ACNGT 7 cut(s) 606, 622, 656, 781, 790, 1040, 1153
BstACI GRCGYC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 1 cut(s) 1143
BstDSI CCRYGG 2 cut(s) 718, 1000
BstF5I GGATG 2 cut(s) 766, 996
BstKTI GATC 3 cut(s) 693, 919, 1068
BstMAI GTCTC 3 cut(s) 67, 664, 832
BstMBI GATC 3 cut(s) 690, 916, 1065
BstMCI CGRYCG 2 cut(s) 198, 1110
BstMWI GCNNNNNNNGC 3 cut(s) 270, 420, 1112
BstNI CCWGG 4 cut(s) 672, 714, 933, 951
BstNSI RCATGY 2 cut(s) 341, 708
BstSCI CCNGG 5 cut(s) 192, 670, 712, 931, 949
BstSFI CTRYAG 2 cut(s) 1039, 1172
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 217
BstXI CCANNNNNNTGG 1 cut(s) 1133
BstZI CGGCCG 2 cut(s) 195, 1107
BsuI GTATCC 1 cut(s) 1062
BsuRI GGCC 7 cut(s) 89, 114, 152, 170, 197, 717, 1109
BtgI CCRYGG 2 cut(s) 718, 1000
BtgZI GCGATG 1 cut(s) 993
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 2 cut(s) 766, 996
BtsIMutI CAGTG 3 cut(s) 384, 618, 786
Cac8I GCNNGC 1 cut(s) 271
Cfr10I RCCGGY 5 cut(s) 269, 314, 644, 800, 1130
Cfr13I GGNCC 5 cut(s) 150, 233, 668, 710, 748
CseI GACGC 1 cut(s) 604
Csp6I GTAC 2 cut(s) 804, 1149
CspAI ACCGGT 2 cut(s) 644, 800
CviAII CATG 8 cut(s) 147, 237, 338, 705, 719, 831, 1119, 1127
CviQI GTAC 2 cut(s) 804, 1149
DdeI CTNAG 1 cut(s) 1143
DpnI GATC 3 cut(s) 692, 918, 1067
DpnII GATC 3 cut(s) 690, 916, 1065
DraIII CACNNNGTG 1 cut(s) 883
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
EaeI YGGCCR 5 cut(s) 87, 112, 168, 195, 1107
EagI CGGCCG 2 cut(s) 195, 1107
EclXI CGGCCG 2 cut(s) 195, 1107
Eco130I CCWWGG 1 cut(s) 718
Eco31I GGTCTC 1 cut(s) 832
Eco47I GGWCC 4 cut(s) 233, 668, 710, 748
Eco52I CGGCCG 2 cut(s) 195, 1107
Eco57I CTGAAG 1 cut(s) 211
Eco88I CYCGRG 2 cut(s) 358, 610
EcoRI GAATTC 1 cut(s) 366
EcoRII CCWGG 4 cut(s) 670, 712, 931, 949
EcoT14I CCWWGG 1 cut(s) 718
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 8 cut(s) 150, 240, 341, 708, 722, 834, 1122, 1130
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 3 cut(s) 176, 268, 1018
FatI CATG 8 cut(s) 146, 236, 337, 704, 718, 830, 1118, 1126
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 2 cut(s) 526, 1107
FokI GGATG 2 cut(s) 773, 983
Fsp4HI GCNGC 2 cut(s) 526, 1107
FspBI CTAG 2 cut(s) 740, 843
GluI GCNGC 2 cut(s) 526, 1107
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 7 cut(s) 89, 114, 152, 170, 197, 717, 1109
HapII CCGG 9 cut(s) 57, 194, 198, 270, 315, 567, 645, 801, 1131
HgaI GACGC 1 cut(s) 604
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 8 cut(s) 150, 240, 341, 708, 722, 834, 1122, 1130
HindIII AAGCTT 2 cut(s) 156, 1113
HinfI GANTC 5 cut(s) 7, 122, 300, 591, 629
HpaII CCGG 9 cut(s) 57, 194, 198, 270, 315, 567, 645, 801, 1131
HphI GGTGA 2 cut(s) 659, 1039
Hpy166II GTNNAC 1 cut(s) 710
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 5 cut(s) 371, 472, 626, 963, 992
Hpy8I GTNNAC 1 cut(s) 710
Hpy99I CGWCG 2 cut(s) 292, 590
HpyAV CCTTC 2 cut(s) 145, 195
HpyCH4III ACNGT 7 cut(s) 606, 622, 656, 781, 790, 1040, 1153
HpyCH4IV ACGT 2 cut(s) 534, 597
HpyCH4V TGCA 9 cut(s) 71, 146, 221, 402, 528, 544, 641, 704, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 270, 420, 1112
HpyF3I CTNAG 1 cut(s) 1143
HpySE526I ACGT 2 cut(s) 534, 597
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 8 cut(s) 150, 240, 341, 708, 722, 834, 1122, 1130
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 3 cut(s) 690, 916, 1065
LmnI GCTCC 2 cut(s) 98, 431
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 2 cut(s) 740, 843
MaeII ACGT 2 cut(s) 534, 597
MaeIII GTNAC 9 cut(s) 73, 317, 569, 616, 647, 775, 790, 982, 1058
MalI GATC 3 cut(s) 692, 918, 1067
MbiI CCGCTC 1 cut(s) 477
MboI GATC 3 cut(s) 690, 916, 1065
MboII GAAGA 2 cut(s) 37, 217
MfeI CAATTG 1 cut(s) 663
MluCI AATT 9 cut(s) 366, 636, 663, 682, 822, 922, 972, 1031, 1090
MlyI GAGTC 3 cut(s) 116, 309, 623
MmeI TCCRAC 5 cut(s) 270, 477, 483, 625, 898
MnlI CCTC 8 cut(s) 47, 175, 187, 496, 574, 619, 751, 1138
MroNI GCCGGC 1 cut(s) 269
MseI TTAA 1 cut(s) 825
MslI CAYNNNNRTG 4 cut(s) 278, 342, 470, 1131
MspA1I CMGCKG 2 cut(s) 231, 429
MspI CCGG 9 cut(s) 57, 194, 198, 270, 315, 567, 645, 801, 1131
MspR9I CCNGG 5 cut(s) 194, 672, 714, 933, 951
MunI CAATTG 1 cut(s) 663
MvaI CCWGG 4 cut(s) 672, 714, 933, 951
MwoI GCNNNNNNNGC 3 cut(s) 270, 420, 1112
NaeI GCCGGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 718
NdeII GATC 3 cut(s) 690, 916, 1065
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 8 cut(s) 150, 240, 341, 708, 722, 834, 1122, 1130
NlaIV GGNNCC 2 cut(s) 100, 676
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 3 cut(s) 616, 647, 982
NspI RCATGY 2 cut(s) 341, 708
OliI CACNNNNGTG 1 cut(s) 278
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 594
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 7, 591
PflFI GACNNNGTC 1 cut(s) 656
PflMI CCANNNNNTGG 1 cut(s) 719
PfoI TCCNGGA 1 cut(s) 670
PinAI ACCGGT 2 cut(s) 644, 800
PkrI GCNGC 2 cut(s) 527, 1108
PleI GAGTC 3 cut(s) 116, 308, 623
PpsI GAGTC 3 cut(s) 116, 308, 623
Psp6I CCWGG 4 cut(s) 670, 712, 931, 949
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 4 cut(s) 670, 712, 931, 949
PspN4I GGNNCC 2 cut(s) 100, 676
PspPI GGNCC 5 cut(s) 150, 233, 668, 710, 748
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 2 cut(s) 805, 1150
RsaNI GTAC 2 cut(s) 804, 1149
RseI CAYNNNNRTG 4 cut(s) 278, 342, 470, 1131
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 2 cut(s) 526, 1107
Sau3AI GATC 3 cut(s) 690, 916, 1065
Sau96I GGNCC 5 cut(s) 150, 233, 668, 710, 748
SchI GAGTC 3 cut(s) 116, 309, 623
ScrFI CCNGG 5 cut(s) 194, 672, 714, 933, 951
SfcI CTRYAG 2 cut(s) 1039, 1172
SinI GGWCC 4 cut(s) 233, 668, 710, 748
SmiMI CAYNNNNRTG 4 cut(s) 278, 342, 470, 1131
Sse9I AATT 9 cut(s) 366, 636, 663, 682, 822, 922, 972, 1031, 1090
SsiI CCGC 5 cut(s) 231, 429, 477, 1025, 1106
SspMI CTAG 2 cut(s) 740, 843
StyD4I CCNGG 5 cut(s) 192, 670, 712, 931, 949
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 7 cut(s) 606, 622, 656, 781, 790, 1040, 1153
TaiI ACGT 2 cut(s) 537, 600
TaqI TCGA 4 cut(s) 627, 693, 891, 1035
TaqII GACCGA 1 cut(s) 134
TasI AATT 9 cut(s) 366, 636, 663, 682, 822, 922, 972, 1031, 1090
TauI GCSGC 1 cut(s) 1109
TfiI GAWTC 2 cut(s) 7, 591
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 3 cut(s) 391, 625, 786
TseFI GTSAC 3 cut(s) 616, 647, 982
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 3 cut(s) 616, 647, 982
TspDTI ATGAA 2 cut(s) 37, 1107
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 3 cut(s) 391, 625, 786
Tth111I GACNNNGTC 1 cut(s) 656
Van91I CCANNNNNTGG 1 cut(s) 719
VpaK11BI GGWCC 4 cut(s) 233, 668, 710, 748
XapI RAATTY 2 cut(s) 366, 1031
XceI RCATGY 2 cut(s) 341, 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XspI CTAG 2 cut(s) 740, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.