Rorug01G0455200

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000702
Physical Location & Seq
Reverse (-)
54975428 .. 54982656
7229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0455200.1

Sequence Viewer

Length: 3957 bp
ATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAGCAGCTTGGACTCAAAGTTGCACGAAGGAAAAGATTGCGCCATGCACAGGAATCTGTTTCTGCAGCCCAGTCAAACTGATGGAGATGGAGAGGAAACAGTTATTACTCATCATGAATCAAAAACTCGGCCTTTGTTTAAAGACCATAAAAGTTGCAACCACATGATTGCCAATGCAATGGATGGGGATGATAAATTGACAGCTCCTCAAGTTACTCGTAAACTTAAGCAGCTTGGTTTATATATTCCTTGGAAGAAGAGGTCTGGAGCTAACATGCTGATGAGGGATCAGGACCTAAATGATTCCAACAATGTGAATGCCTCTGATGATGAAACATTGTTATCATTAATGAACAGGGGCAAAAAGGATAATATCAGAATTAGTGAGGTACCAGAACAGATTACTGGAAGAGAAGTGGCAGAAGATGAGTCTGATGATGAAATGCTAAGCTCTGTTCTCAAGAAAGCTAAGGGATCCCTTTCTAAATTAAAGGATCAAAATCTCACAGCCATCTTGATTCAGGGAAATGCTTTAGAAAATAAAGTTGAGGAAGGTGTTGTATCTTCTGGCAGATTCAAGGACAACCACAATTCTAGTCACCTTATTGCTGAAGCTCTTGATCCTGATGGGAAAATTTCATCAACTCAAATTTCCAACAAGCTTAAACAGCTTGGACTCAAAGTTGCGCGAAGGAAAAGATTGCGCCATGCACAGGAATCTGTTTCTGCTGAGCCTAGTCAAACTGATGGAGATGGAGAAGTAGAGGAAACAGTTATTACTCATCATGAATCAAAAACCCGGCCTTTGCACACAAGAAAAAGAGTCTCTGGCTTAAGCGAAGAACAGGAAGCTCGAATTAGATCTCTTTATGATCAGTTTAAAGACCATAACAGTTGCAACCACATGATTGTCACTGCAATGGATGGGGATGATACATTGATAGCTCCTCAAGTTTCTTGTAAACTTAAGCAGCTTGGGTTATATATTCCTCGGAAGAAGAGATCATCCATACATTCTGGTTCTGTGTTGGCTCGGCTTCTATGGCTGGTCATCGTTTCGTCTGCTGCTACCTCCTGTTGTTGTTCCAGTGCTGCTGGGAATATCAGTACTTCGAACGATGTCAATGTTCGGTGTATGGAGAGGGAAAAACATGCTCTTCTTCAGTTCAAACAAGGGTTGGTGGATGGCTCCAATGCTCTTGCCTCTTGGGAAAGCAAGGAAAACTGCTGCGAGTGGAGAGGAGTTGCATGCGACAACCAAACAGGTCATGTCATCATGCTAGATCTCTACTATAATTCATCTGATATTAATGATAATACTCCTTTAAGTGGGGAGATTGGTCCCTCACTACTTGAATTGCCATATCTATATTACTTGGACCTCGGTTTTAATTCTTTAAATGGAACGATTCCCAAGTTCTTCGGTTCTTTGAGTCAGTTGAAAGTACTCAAACTCGGATCCAACAGTTTTAGCGGAACCATTCCTCCCCAACTTGGCAATCTATCCAATTTGCATACTCTTGATCTTTCATGGAACACTGTCACTTCTAAAAATCTCGAGTGGGTATCTCAGCTTTCTTCCTTGAGATACTTGGACATGTCATATCTTAATTTCTCTTGTGCTCTAAATTGGCCCCAATCTATAAGCCAGCTCCCTTCACTGATAGAGCTTCAGTTAGAATCATGTGATCTTCCTGATGTTGATATGGCATCACTTTCCTTTATTAATTCTTCGACCTCTCTCCAAGTATTTCATCTCCCTCTTAATTTCCTCAGTTCTTCAGTATTTTGTTGGATAGGTAATGTCAGCAAGAACCTTGTTTCCATTGACCTATCTAATAGCAGGATTAAAGGTCCTATCCCAGATGTTTTCACAAACATGGATTCTCTGTTATCTCTTGACCTCAATTGTAATCAACTTGAAGGTGGCTTACCAAATTCCTTTCGAAGCCTATGCAGATTGGAAAAATTGAATTTATCGTCAAACAATTTTTCTGACAGACTTGAACAATCTATAGGCAACTTGTCTTGTGCACAGGATACCCTTAAGTCATTATACTTGGGTGGAAATATGTGGTGGGGGTCATTTCCTAATGATCTTACAAGGTTTGTATCCTTGGAAGAATTGGACATTGACAATGCCACTATGAGCGGGTCGCTCCCAAAGAGTTTTCGGCAACTATCCCAGCTAAGAACCTTGCGTCTACCTCATAACCGGTTAAGTGGCTCATTCCCAGATGTTACAGGTCTTTCATTGTTATCATGGATAAATCTTTCTGATAATCAACTGAATGGCTCTCTACATGACAGTTTAGGTCAACTATCCAGCCTTACAGAAATCGATCTTTCTCGAAATTCTCTGAATGTTGTCTTAACTGAAGCCCACTTCTTGAACCTGTCTAAGCTACAGTTTGTGGATGTTTCTTATAATCCAGCTTTTTCTTTCCATGTGAGCTTAAGTTGGAATCCACCTTTTCAGCTTATAAGTTTAAAAATGGCATCTTGCAAGGTCGGCCCTGCTTTTCCTAAATGGATACATACACAGAGACAACTTTGGGCGCTTGATTTGTCTAATGCTGGGATATCAGATTCAATACCTTACGAGTTTTGGGAACTATCTTGCAGCTTATATCAGTTAGATCTCTCTACCAACCAAATCCATGGAAGTTTTCCGAATCCATCAGCAAGGAACCGCACCATTGCTTCTATTGACTTGAGTTCTAATCAGTTGTCTGGTGCACTCCCACCATTTCTTATGAGTGTAGGTTCATTACTGTTGCTCTCCAAAAACAAGTTTACAGGAGACCTATCTTCCATATGTTCAACTAGGGCTCCATATTTGTATATACTCGACCTTTCTCATAATCTGCTATCAGGGGAGCTCCCTAATTGTTGGGTTCAATTTCAAGAACTGATCGTGTTGAATTTGGCAAACAATAGTTTCTCTGGGAAAATACCAAGCTCATTTGGTTCTTTAAAGTATCTTTCTCAATTACAATTACAACGTAACAATTTTTCAGGTGAACTGCCTTCTCTGGAGAACTGTACGCTGTTACTTTTGGTTGACCTTGGATTCAATAACTTATCTGGAAAGATACAAGCATGGATAGGCAGCAGCCTTCAAAAGTTGTGGGTTCTACAATTAAGGTCAAACCACTTCTCTGGAAGCATTCCCTCAAGTATATGCAGTCTGCCTCTCATTCGTGTTTTGGACCTCTCTCAAAACAATATATCTGGAGTATTGCCACATTGCCTCAACAATTTGACAGCTTTCTCTTCGGTTTCTCAGGAGGTGGATGATTTTCCATTTCCTTTCTCAGATCATGATTTTGTTTTACAACTTATTTGGAAAGGAGCAGAGACGGAGTATGGGAAAAACTTTAGGTATTTGAGGAGCATTGACATTTCAAGCAATAACTTAACTGGGGTGATTCCGGAAAACATAACATATATGCTTGGGTTACGTTCTCTTAACCTTTCAAGAAACAATTTGACTGGGGTGATTCCTGGAAAGTTTGGTCAGTTGGAGGTGCTTGAAGTTCTTGATTTGTCAAGAAACCACCTATCTGGTAGTATTCCTGATAGTTTTAGGAACTTGCATTCTATAGCTGTCTTGAATTTATCGATCAACAACTTGTCAGGAAGAATTCCATCCAGCACCCAACTTCTGACTTTTACTGAATCTTCATTCGCTGGAAATGTTGGACTTTGCGGGCCACCGCTAACAACAAAATGCCCCGGAGATAGACCGAGTGAAGATCCAAAAGTCACTGGTGGCACTGAAAGTGATGGTTTCATAACACCAGGGTTTTATGCCGGCATAGGGATTGGATTCTTAATAGGATTTGTGGGAGTCTGCCTTGCTTTACCGCCCGAGACTTCTTGGAGATACGTACAGCAGTGTTTAAGGCAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

1318

Amino Acids

145.68

Weight (kDa)

6.08

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 394 - 431 3.2e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 460 - 579 7.9e-08 Leucine-rich repeat region
LRR_8 PF13855 467 - 525 7e-08 Leucine rich repeat
LRR_8 PF13855 618 - 677 1.2e-09 Leucine rich repeat
LRR_14 PF23598 706 - 867 1.4e-11 Leucine-rich repeat region
LRR_8 PF13855 1142 - 1201 1.2e-12 Leucine rich repeat
LRR_8 PF13855 1167 - 1225 1.6e-11 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000702)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43870 AT3G59850 AT3G59850
fragaria_vesca FvH4_1g00290 FvH4_1g00290 FvH4_1g21842 FvH4_1g21850 FvH4_1g21851
malus_domestica MD00G1070500.v1.1 MD00G1087900.v1.1
prunus_persica Prupe.4G261700_v2.0.a1 Prupe.4G261800_v2.0.a1 Prupe.4G261900_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.4G262200_v2.0.a1 Prupe.7G269200_v2.0.a1
pyrus_communis pycom02g00030 pycom03g19680
rosa_chinensis RchiOBHm_Chr2g0084591 RchiOBHm_Chr2g0085391 RchiOBHm_Chr2g0115741 RchiOBHm_Chr2g0115771 RchiOBHm_Chr2g0115801 RchiOBHm_Chr2g0115811 RchiOBHm_Chr2g0115821 RchiOBHm_Chr5g0021021 RchiOBHm_Chr5g0021031
rosa_laevigata RLG00000015683 RLG00000018208 RLG00000018211 RLG00000018212
rosa_multiflora Rmu_co8137638.1_g000001 Rmu_sc0000742.1_g000006 Rmu_sc0004926.1_g000013 Rmu_sc0008355.1_g000010 Rmu_sc0011452.1_g000015 Rmu_ssc0000197.1_g000031 Rmu_ssc0000197.1_g000032 Rmu_ssc0000197.1_g000056
rosa_roxburghii Rroxscaffold_2G00127770 Rroxscaffold_2G00127780 Rroxscaffold_2G00127790 Rroxscaffold_2G00127830 Rroxscaffold_2G00127850 Rroxscaffold_2G00128630 Rroxscaffold_2G00128640 Rroxscaffold_2G00128680 Rroxscaffold_2G00128730 Rroxscaffold_2G00128740
rosa_rugosa Rorug01G0455200 Rorug01G0460100 Rorug02G0199800 Rorug02G0199900
rosa_samantha Rh2BG003000 Rh2BG267500 Rh2BG267700 Rh2BG267800 Rh2BG267900 Rh2CG003400 Rh2CG010000 Rh2CG262800 Rh2CG263200 Rh2CG263300 Rh2CG263400 Rh5CG165700 Rh5CG165800
rosa_wichuraiana Rw0G014320 Rw0G014330 Rw1G010220 Rw2G000800 Rw2G020110 Rw2G020130 Rw2G020150 Rw2G020160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2466, 2522
Acc65I GGTACC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 2190
AccI GTMKAC 1 cut(s) 2242
AccII CGCG 1 cut(s) 727
AccIII TCCGGA 1 cut(s) 3472
AciI CCGC 6 cut(s) 1512, 2190, 2731, 3750, 3758, 3908
AclWI GGATC 8 cut(s) 333, 507, 520, 540, 653, 1491, 1504, 3791
AcuI CTGAAG 5 cut(s) 669, 1184, 1694, 1803, 2436
AfaI GTAC 5 cut(s) 429, 1147, 1485, 3085, 3933
AfiI CCNNNNNNNGG 5 cut(s) 87, 751, 1367, 1532, 3861
AflII CTTAAG 6 cut(s) 37, 263, 871, 1004, 2084, 2494
AflIII ACRYGT 1 cut(s) 1635
AgeI ACCGGT 1 cut(s) 2253
AjnI CCWGG 2 cut(s) 3544, 3841
AloI GAACNNNNNNTCC 4 cut(s) 1507, 1539, 1844, 1876
Alw21I GWGCWC 4 cut(s) 1663, 2074, 2779, 2922
Alw26I GTCTC 5 cut(s) 868, 2578, 2835, 3392, 3908
Alw44I GTGCAC 2 cut(s) 2070, 2775
AlwI GGATC 8 cut(s) 333, 507, 520, 540, 653, 1491, 1504, 3791
Ama87I CYCGRG 2 cut(s) 1595, 3911
Aor13HI TCCGGA 1 cut(s) 3472
AoxI GGCC 5 cut(s) 167, 839, 1670, 2551, 3752
ApaLI GTGCAC 2 cut(s) 2070, 2775
AseI ATTAAT 3 cut(s) 386, 1347, 1764
AsiGI ACCGGT 1 cut(s) 2253
Asp718I GGTACC 1 cut(s) 427
AspLEI GCGC 4 cut(s) 80, 727, 744, 2599
AspS9I GGNCC 8 cut(s) 331, 1379, 1417, 1671, 1892, 2552, 3250, 3752
AsuC2I CCSGG 2 cut(s) 838, 3777
AsuHPI GGTGA 4 cut(s) 629, 3071, 3478, 3550
AsuII TTCGAA 2 cut(s) 1151, 1984
AvaI CYCGRG 2 cut(s) 1595, 3911
AvaII GGWCC 5 cut(s) 331, 1379, 1417, 1892, 3250
BaeGI GKGCMC 2 cut(s) 2074, 2779
BamHI GGATCC 2 cut(s) 512, 1496
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 2872, 2922
BarI GAAGNNNNNNTAC 2 cut(s) 1953, 1985
Bbv12I GWGCWC 4 cut(s) 1663, 2074, 2779, 2922
BcgI CGANNNNNNTGC 2 cut(s) 1974, 2008
BciT130I CCWGG 2 cut(s) 3546, 3843
BciVI GTATCC 3 cut(s) 2071, 2161, 2565
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 2 cut(s) 838, 3777
BcoDI GTCTC 5 cut(s) 868, 2578, 2835, 3392, 3908
BfaI CTAG 4 cut(s) 633, 774, 1319, 2865
BfmI CTRYAG 4 cut(s) 101, 2052, 2444, 3642
BfoI RGCGCY 1 cut(s) 2600
BfrI CTTAAG 6 cut(s) 37, 263, 871, 1004, 2084, 2494
BfuI GTATCC 3 cut(s) 2071, 2161, 2565
BglII AGATCT 3 cut(s) 899, 1321, 2677
BlpI GCTNAGC 2 cut(s) 485, 768
BmcAI AGTACT 2 cut(s) 1147, 1485
Bme1390I CCNGG 4 cut(s) 838, 3546, 3777, 3843
Bme18I GGWCC 5 cut(s) 331, 1379, 1417, 1892, 3250
BmeT110I CYCGRG 2 cut(s) 1595, 3911
BmgT120I GGNCC 8 cut(s) 331, 1379, 1417, 1671, 1892, 2552, 3250, 3752
BmiI GGNNCC 9 cut(s) 429, 514, 1228, 1381, 1498, 1516, 1673, 2729, 2871
BmrFI CCNGG 4 cut(s) 838, 3546, 3777, 3843
BmrI ACTGGG 3 cut(s) 102, 3471, 3543
BmsI GCATC 2 cut(s) 1757, 2546
BmuI ACTGGG 3 cut(s) 102, 3471, 3543
BpmI CTGGAG 3 cut(s) 324, 3095, 3294
Bpu10I CCTNAGC 1 cut(s) 507
Bpu1102I GCTNAGC 2 cut(s) 485, 768
Bpu14I TTCGAA 2 cut(s) 1151, 1984
BpuEI CTTGAG 6 cut(s) 231, 482, 972, 1642, 2773, 3199
BpuMI CCSGG 2 cut(s) 838, 3777
Bsa29I ATCGAT 2 cut(s) 2379, 3662
BsaAI YACGTR 1 cut(s) 3931
BsaBI GATNNNNATC 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 2835
BsaJI CCNNGG 8 cut(s) 287, 1028, 1420, 2154, 2698, 3106, 3775, 3842
BsaWI WCCGGW 2 cut(s) 2253, 3472
BsaXI ACNNNNNCTCC 4 cut(s) 1507, 1537, 2854, 2884
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 751, 1367, 1532, 3861
Bse118I RCCGGY 2 cut(s) 2253, 3854
Bse1I ACTGG 6 cut(s) 108, 448, 1125, 3466, 3538, 3814
Bse3DI GCAATG 4 cut(s) 222, 963, 2736, 3286
Bse8I GATNNNNATC 1 cut(s) 537
BseAI TCCGGA 1 cut(s) 3472
BseBI CCWGG 2 cut(s) 3546, 3843
BseCI ATCGAT 2 cut(s) 2379, 3662
BseDI CCNNGG 8 cut(s) 287, 1028, 1420, 2154, 2698, 3106, 3775, 3842
BseGI GGATG 9 cut(s) 226, 232, 967, 973, 1043, 1228, 2461, 3340, 3689
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 5 cut(s) 87, 751, 1367, 1532, 3861
BseMI GCAATG 4 cut(s) 222, 963, 2736, 3286
BseMII CTCAG 5 cut(s) 759, 1622, 1825, 3338, 3369
BseNI ACTGG 6 cut(s) 108, 448, 1125, 3466, 3538, 3814
BseRI GAGGAG 4 cut(s) 234, 975, 1293, 3445
BseSI GKGCMC 2 cut(s) 2074, 2779
BseYI CCCAGC 3 cut(s) 1133, 2223, 2615
Bsh1236I CGCG 1 cut(s) 727
BshFI GGCC 5 cut(s) 169, 841, 1672, 2553, 3754
BshNI GGYRCC 1 cut(s) 427
BshTI ACCGGT 1 cut(s) 2253
BshVI ATCGAT 2 cut(s) 2379, 3662
BsiHKAI GWGCWC 4 cut(s) 1663, 2074, 2779, 2922
BsiHKCI CYCGRG 2 cut(s) 1595, 3911
BsiSI CCGG 5 cut(s) 838, 2254, 3473, 3777, 3855
BslFI GGGAC 1 cut(s) 1365
BslI CCNNNNNNNGG 5 cut(s) 87, 751, 1367, 1532, 3861
BsmAI GTCTC 5 cut(s) 868, 2578, 2835, 3392, 3908
BsmBI CGTCTC 1 cut(s) 3392
BsmFI GGGAC 1 cut(s) 1365
BsmI GAATGC 3 cut(s) 361, 3207, 3637
BsnI GGCC 5 cut(s) 169, 841, 1672, 2553, 3754
Bso31I GGTCTC 1 cut(s) 2835
BsoBI CYCGRG 2 cut(s) 1595, 3911
Bsp119I TTCGAA 2 cut(s) 1151, 1984
Bsp1286I GDGCHC 5 cut(s) 1663, 2074, 2779, 2872, 2922
Bsp13I TCCGGA 1 cut(s) 3472
Bsp1720I GCTNAGC 2 cut(s) 485, 768
Bsp19I CCATGG 1 cut(s) 2698
BspACI CCGC 6 cut(s) 1512, 2190, 2731, 3750, 3758, 3908
BspANI GGCC 5 cut(s) 169, 841, 1672, 2553, 3754
BspCNI CTCAG 5 cut(s) 760, 1621, 1824, 3337, 3368
BspDI ATCGAT 2 cut(s) 2379, 3662
BspEI TCCGGA 1 cut(s) 3472
BspFNI CGCG 1 cut(s) 727
BspHI TCATGA 3 cut(s) 151, 823, 3361
BspLI GGNNCC 9 cut(s) 429, 514, 1228, 1381, 1498, 1516, 1673, 2729, 2871
BspMAI CTGCAG 1 cut(s) 105
BspPI GGATC 8 cut(s) 333, 507, 520, 540, 653, 1491, 1504, 3791
BspQI GCTCTTC 1 cut(s) 1200
BspT104I TTCGAA 2 cut(s) 1151, 1984
BspT107I GGYRCC 1 cut(s) 427
BspTI CTTAAG 6 cut(s) 37, 263, 871, 1004, 2084, 2494
BspTNI GGTCTC 1 cut(s) 2835
BsrBI CCGCTC 1 cut(s) 2190
BsrDI GCAATG 4 cut(s) 222, 963, 2736, 3286
BsrFI RCCGGY 2 cut(s) 2253, 3854
BsrI ACTGG 6 cut(s) 108, 448, 1125, 3466, 3538, 3814
BssAI RCCGGY 2 cut(s) 2253, 3854
BssECI CCNNGG 8 cut(s) 287, 1028, 1420, 2154, 2698, 3106, 3775, 3842
BssT1I CCWWGG 4 cut(s) 287, 2154, 2698, 3106
Bst2UI CCWGG 2 cut(s) 3546, 3843
Bst4CI ACNGT 9 cut(s) 139, 811, 932, 1505, 1579, 2348, 2448, 2814, 3083
Bst6I CTCTTC 5 cut(s) 290, 442, 1031, 1200, 3319
BstAFI CTTAAG 6 cut(s) 37, 263, 871, 1004, 2084, 2494
BstBAI YACGTR 1 cut(s) 3931
BstBI TTCGAA 2 cut(s) 1151, 1984
BstC8I GCNNGC 4 cut(s) 1288, 1688, 3752, 3856
BstDEI CTNAG 9 cut(s) 485, 507, 768, 1608, 1811, 2228, 2439, 3324, 3355
BstDSI CCRYGG 1 cut(s) 2698
BstF5I GGATG 9 cut(s) 226, 232, 967, 973, 1043, 1228, 2461, 3340, 3689
BstFNI CGCG 1 cut(s) 727
BstH2I RGCGCY 1 cut(s) 2600
BstHHI GCGC 4 cut(s) 80, 727, 744, 2599
BstMAI GTCTC 5 cut(s) 868, 2578, 2835, 3392, 3908
BstMWI GCNNNNNNNGC 4 cut(s) 42, 706, 1081, 2550
BstNI CCWGG 2 cut(s) 3546, 3843
BstNSI RCATGY 4 cut(s) 316, 1193, 1290, 1639
BstSCI CCNGG 4 cut(s) 836, 3544, 3775, 3841
BstSFI CTRYAG 4 cut(s) 101, 2052, 2444, 3642
BstSLI GKGCMC 2 cut(s) 2074, 2779
BstSNI TACGTA 1 cut(s) 3931
BstUI CGCG 1 cut(s) 727
BstX2I RGATCY 6 cut(s) 512, 899, 1321, 1496, 2677, 3796
BstXI CCANNNNNNTGG 4 cut(s) 217, 2699, 3200, 3605
BstYI RGATCY 6 cut(s) 512, 899, 1321, 1496, 2677, 3796
Bsu15I ATCGAT 2 cut(s) 2379, 3662
BsuI GTATCC 3 cut(s) 2071, 2161, 2565
BsuRI GGCC 5 cut(s) 169, 841, 1672, 2553, 3754
BsuTUI ATCGAT 2 cut(s) 2379, 3662
BtgI CCRYGG 1 cut(s) 2698
BtsCI GGATG 9 cut(s) 226, 232, 967, 973, 1043, 1228, 2461, 3340, 3689
BtsI GCAGTG 2 cut(s) 951, 3944
BtsIMutI CAGTG 7 cut(s) 951, 1132, 1575, 1697, 3807, 3816, 3944
Cac8I GCNNGC 4 cut(s) 1288, 1688, 3752, 3856
CciI TCATGA 3 cut(s) 151, 823, 3361
CfoI GCGC 4 cut(s) 80, 727, 744, 2599
Cfr10I RCCGGY 2 cut(s) 2253, 3854
Cfr13I GGNCC 8 cut(s) 331, 1379, 1417, 1671, 1892, 2552, 3250, 3752
ClaI ATCGAT 2 cut(s) 2379, 3662
CseI GACGC 1 cut(s) 2228
Csp6I GTAC 5 cut(s) 428, 1146, 1484, 3084, 3932
CspAI ACCGGT 1 cut(s) 2253
CspCI CAANNNNNGTGG 2 cut(s) 3149, 3184
CviQI GTAC 5 cut(s) 428, 1146, 1484, 3084, 3932
DdeI CTNAG 9 cut(s) 485, 507, 768, 1608, 1811, 2228, 2439, 3324, 3355
DraI TTTAAA 5 cut(s) 178, 919, 1437, 2529, 3015
Eam1104I CTCTTC 5 cut(s) 290, 442, 1031, 1200, 3319
EarI CTCTTC 5 cut(s) 290, 442, 1031, 1200, 3319
Ecl136II GAGCTC 1 cut(s) 2920
Eco105I TACGTA 1 cut(s) 3931
Eco130I CCWWGG 4 cut(s) 287, 2154, 2698, 3106
Eco24I GRGCYC 2 cut(s) 2872, 2922
Eco31I GGTCTC 1 cut(s) 2835
Eco32I GATATC 1 cut(s) 2622
Eco47I GGWCC 5 cut(s) 331, 1379, 1417, 1892, 3250
Eco53kI GAGCTC 1 cut(s) 2920
Eco57I CTGAAG 5 cut(s) 669, 1184, 1694, 1803, 2436
Eco88I CYCGRG 2 cut(s) 1595, 3911
EcoICRI GAGCTC 1 cut(s) 2920
EcoO109I RGGNCCY 2 cut(s) 331, 1892
EcoRI GAATTC 1 cut(s) 3684
EcoRII CCWGG 2 cut(s) 3544, 3841
EcoRV GATATC 1 cut(s) 2622
EcoT14I CCWWGG 4 cut(s) 287, 2154, 2698, 3106
EcoT38I GRGCYC 2 cut(s) 2872, 2922
ErhI CCWWGG 4 cut(s) 287, 2154, 2698, 3106
Esp3I CGTCTC 1 cut(s) 3392
FaqI GGGAC 1 cut(s) 1365
FauI CCCGC 2 cut(s) 2183, 3743
FauNDI CATATG 1 cut(s) 2855
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 1 cut(s) 2242
FokI GGATG 9 cut(s) 233, 239, 974, 980, 1030, 1235, 2468, 3347, 3676
FriOI GRGCYC 2 cut(s) 2872, 2922
FspBI CTAG 4 cut(s) 633, 774, 1319, 2865
GlaI GCGC 4 cut(s) 79, 726, 743, 2598
GsaI CCCAGC 3 cut(s) 1137, 2227, 2619
GsuI CTGGAG 3 cut(s) 324, 3095, 3294
HaeII RGCGCY 1 cut(s) 2600
HaeIII GGCC 5 cut(s) 169, 841, 1672, 2553, 3754
HapII CCGG 5 cut(s) 838, 2254, 3473, 3777, 3855
HgaI GACGC 1 cut(s) 2228
HhaI GCGC 4 cut(s) 80, 727, 744, 2599
Hin6I GCGC 4 cut(s) 78, 725, 742, 2597
HinP1I GCGC 4 cut(s) 78, 725, 742, 2597
HincII GTYRAC 2 cut(s) 2357, 3103
HindII GTYRAC 2 cut(s) 2357, 3103
HindIII AAGCTT 2 cut(s) 34, 698
HpaII CCGG 5 cut(s) 838, 2254, 3473, 3777, 3855
HphI GGTGA 4 cut(s) 629, 3071, 3478, 3550
Hpy166II GTNNAC 9 cut(s) 260, 1001, 2072, 2243, 2357, 2777, 2835, 3062, 3103
Hpy8I GTNNAC 9 cut(s) 260, 1001, 2072, 2243, 2357, 2777, 2835, 3062, 3103
HpyAV CCTTC 7 cut(s) 59, 584, 723, 1704, 1955, 3078, 3167
HpyCH4III ACNGT 9 cut(s) 139, 811, 932, 1505, 1579, 2348, 2448, 2814, 3083
HpyCH4IV ACGT 3 cut(s) 3043, 3502, 3930
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 706, 1081, 2550
HpyF3I CTNAG 9 cut(s) 485, 507, 768, 1608, 1811, 2228, 2439, 3324, 3355
HpySE526I ACGT 3 cut(s) 3043, 3502, 3930
HspAI GCGC 4 cut(s) 78, 725, 742, 2597
Kpn2I TCCGGA 1 cut(s) 3472
KpnI GGTACC 1 cut(s) 431
KroI GCCGGC 1 cut(s) 3854
KroNI GCCGGC 1 cut(s) 3856
Ksp22I TGATCA 1 cut(s) 910
LguI GCTCTTC 1 cut(s) 1200
LweI GCATC 2 cut(s) 1757, 2546
MaeI CTAG 4 cut(s) 633, 774, 1319, 2865
MaeII ACGT 3 cut(s) 3043, 3502, 3930
MaeIII GTNAC 9 cut(s) 250, 635, 949, 1579, 2278, 3044, 3090, 3498, 3805
MbiI CCGCTC 1 cut(s) 2190
MfeI CAATTG 1 cut(s) 1945
MflI RGATCY 6 cut(s) 512, 899, 1321, 1496, 2677, 3796
MhlI GDGCHC 5 cut(s) 1663, 2074, 2779, 2872, 2922
MlyI GAGTC 6 cut(s) 44, 476, 708, 870, 1480, 3900
MmeI TCCRAC 8 cut(s) 53, 369, 717, 1524, 1811, 2480, 3543, 3721
MroI TCCGGA 1 cut(s) 3472
MroNI GCCGGC 1 cut(s) 3854
MslI CAYNNNNRTG 3 cut(s) 317, 956, 1916
MspCI CTTAAG 6 cut(s) 37, 263, 871, 1004, 2084, 2494
MspI CCGG 5 cut(s) 838, 2254, 3473, 3777, 3855
MspR9I CCNGG 4 cut(s) 838, 3546, 3777, 3843
MunI CAATTG 1 cut(s) 1945
Mva1269I GAATGC 3 cut(s) 361, 3207, 3637
MvaI CCWGG 2 cut(s) 3546, 3843
MvnI CGCG 1 cut(s) 727
MwoI GCNNNNNNNGC 4 cut(s) 42, 706, 1081, 2550
NaeI GCCGGC 1 cut(s) 3856
NciI CCSGG 2 cut(s) 838, 3777
NcoI CCATGG 1 cut(s) 2698
NdeI CATATG 1 cut(s) 2855
NgoMIV GCCGGC 1 cut(s) 3854
NlaIV GGNNCC 9 cut(s) 429, 514, 1228, 1381, 1498, 1516, 1673, 2729, 2871
NmeAIII GCCGAG 2 cut(s) 145, 1051
NmuCI GTSAC 4 cut(s) 635, 949, 1579, 3805
NspI RCATGY 4 cut(s) 316, 1193, 1290, 1639
NspV TTCGAA 2 cut(s) 1151, 1984
PaeI GCATGC 1 cut(s) 1290
PaeR7I CTCGAG 1 cut(s) 1595
PagI TCATGA 3 cut(s) 151, 823, 3361
PciI ACATGT 1 cut(s) 1635
PciSI GCTCTTC 1 cut(s) 1200
PctI GAATGC 3 cut(s) 361, 3207, 3637
PdiI GCCGGC 1 cut(s) 3856
PfoI TCCNGGA 1 cut(s) 3544
PinAI ACCGGT 1 cut(s) 2253
PleI GAGTC 6 cut(s) 44, 475, 708, 869, 1479, 3899
PpsI GAGTC 6 cut(s) 44, 475, 708, 869, 1479, 3899
Ppu21I YACGTR 1 cut(s) 3931
PpuMI RGGWCCY 2 cut(s) 331, 1892
PscI ACATGT 1 cut(s) 1635
PshBI ATTAAT 3 cut(s) 386, 1347, 1764
PsiI TTATAA 2 cut(s) 2466, 2522
Psp124BI GAGCTC 1 cut(s) 2922
Psp5II RGGWCCY 2 cut(s) 331, 1892
Psp6I CCWGG 2 cut(s) 3544, 3841
PspFI CCCAGC 3 cut(s) 1133, 2223, 2615
PspGI CCWGG 2 cut(s) 3544, 3841
PspN4I GGNNCC 9 cut(s) 429, 514, 1228, 1381, 1498, 1516, 1673, 2729, 2871
PspPI GGNCC 8 cut(s) 331, 1379, 1417, 1671, 1892, 2552, 3250, 3752
PspPPI RGGWCCY 2 cut(s) 331, 1892
PstI CTGCAG 1 cut(s) 105
PsuI RGATCY 6 cut(s) 512, 899, 1321, 1496, 2677, 3796
RsaI GTAC 5 cut(s) 429, 1147, 1485, 3085, 3933
RsaNI GTAC 5 cut(s) 428, 1146, 1484, 3084, 3932
RseI CAYNNNNRTG 3 cut(s) 317, 956, 1916
SacI GAGCTC 1 cut(s) 2922
SapI GCTCTTC 1 cut(s) 1200
Sau96I GGNCC 8 cut(s) 331, 1379, 1417, 1671, 1892, 2552, 3250, 3752
ScaI AGTACT 2 cut(s) 1147, 1485
SchI GAGTC 6 cut(s) 44, 476, 708, 870, 1480, 3900
ScrFI CCNGG 4 cut(s) 838, 3546, 3777, 3843
SduI GDGCHC 5 cut(s) 1663, 2074, 2779, 2872, 2922
SfaNI GCATC 2 cut(s) 1757, 2546
SfcI CTRYAG 4 cut(s) 101, 2052, 2444, 3642
Sfr274I CTCGAG 1 cut(s) 1595
SfuI TTCGAA 2 cut(s) 1151, 1984
SinI GGWCC 5 cut(s) 331, 1379, 1417, 1892, 3250
SlaI CTCGAG 1 cut(s) 1595
SmiMI CAYNNNNRTG 3 cut(s) 317, 956, 1916
SnaBI TACGTA 1 cut(s) 3931
SphI GCATGC 1 cut(s) 1290
SsiI CCGC 6 cut(s) 1512, 2190, 2731, 3750, 3758, 3908
SspMI CTAG 4 cut(s) 633, 774, 1319, 2865
SstI GAGCTC 1 cut(s) 2922
StyD4I CCNGG 4 cut(s) 836, 3544, 3775, 3841
StyI CCWWGG 4 cut(s) 287, 2154, 2698, 3106
TaaI ACNGT 9 cut(s) 139, 811, 932, 1505, 1579, 2348, 2448, 2814, 3083
TaiI ACGT 3 cut(s) 3046, 3505, 3933
TaqI TCGA 9 cut(s) 892, 1151, 1596, 1772, 1984, 2379, 2389, 2889, 3662
TaqII GACCGA 1 cut(s) 3802
TatI WGTACW 2 cut(s) 1145, 1483
TscAI CASTG 7 cut(s) 958, 1132, 1582, 1704, 3814, 3823, 3944
TseFI GTSAC 4 cut(s) 635, 949, 1579, 3805
Tsp45I GTSAC 4 cut(s) 635, 949, 1579, 3805
TspGWI ACGGA 1 cut(s) 3416
TspRI CASTG 7 cut(s) 958, 1132, 1582, 1704, 3814, 3823, 3944
Vha464I CTTAAG 6 cut(s) 37, 263, 871, 1004, 2084, 2494
VneI GTGCAC 2 cut(s) 2070, 2775
VpaK11BI GGWCC 5 cut(s) 331, 1379, 1417, 1892, 3250
VspI ATTAAT 3 cut(s) 386, 1347, 1764
XceI RCATGY 4 cut(s) 316, 1193, 1290, 1639
XhoI CTCGAG 1 cut(s) 1595
XmiI GTMKAC 1 cut(s) 2242
XspI CTAG 4 cut(s) 633, 774, 1319, 2865
ZrmI AGTACT 2 cut(s) 1147, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.