Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000988

Physical Location & Seq

Genomic Coordinates Forward (+)

8737266 .. 8740523

Gene Structure 3258 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g09890.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGAAGAGGATGGGTGTGTTGGGATTACTTTGTGCTTCTACTTGGTCCATACATAGGGCAGCTATATTTGTAGTATTGGGTATGATTTTGAACCTGGGAGTGGTGTGTAATGGAGGCAAAACTAGCACTTTTGTTAGGCAAATTGACAGAGGCTTGGATATGCCTCTTGACAGTGATGTGTTTAAAGTCCCTCCAGGCTACAATGCACCTCAACAAGTTCATATAACGCAAGGCGACCGTCATGGGAAGGGTGTGATTGTGTCATGGGTGACTGTGGATGAACCAGGTTCCAGTACTGTGCTTTACTGGAGTGCAAATGGGAAGCAAATGAAGGCTGAGGGAAAAGTTAAAACCTATAACTTCTACAATTACACTTCTGGTTACATTCACCACACCACAATCAAAAAACTAAAGTTCAATACCAAATATTACTATGTCGTTGGAATCGGCCACACTGAGCGGCAGTTCTGGTTTGTAACTCCTCCTAAAGTTGGTCCAGACGTCCCTTACACCTTTGGTCTCATAGGGGATCTGGGTCAGACCTTTGATTCAAACAAGACACTAACCCATTATGAGAGAAATCCATTGGAAGGAGAAATGGTTTTATTTGTTGGTGACCTCTCGTATGCGGATGACTACCCTGATCATGATAATGTCAGATGGGATACATGGGGAAGATTCACAGAGCGAAGTGTTGCTTATCAACCTTGGATATGGACTGCAGGGAATCATGAAATTGATTTGGCCCCTGAAATTGGTGAAACAAAATCGTTTAAGCCTTACACGCATCGGTATCATGTCCCATATAAAGCATCAGGAAGTACTGCTCCTTATTGGTACTCAATCAAGAGAGCTTCATCCTACATCATTGTCTTAGCTTCATATTCCGCATATGGTAAATACACTCCTCAGTACCTATGGCTTGAGCAAGAGCTATCAAAAGTTAATAGAAGTGAGACACCATGGTTAATTGTCTTAATGCATTCCCCATGGTATAATAGCTATAACTATCACTATATGGAAGGAGAAAGCATGAGAGTGATGTTTGAGCCCTGGTTTGTACAGTACAAAGTCGATGTGGTATTCTCTGGTCATGTCCATGCCTATGAACGATCTGAACGCATATCCAATGTTGCATATAACATTACAAATGGTAATTGCATTCCTGTGAAAGATCAATCTGCTCCTGTGTATATTACCATTGGGGATGGAGGAAATCTTGAAGGCTTAGCAACCTCCATGACAGAGCCACAGCCCGAGTACTCGGCCTATCGAGAGGCCAGCTTTGGTCATGCCATTTTTGACATCAAGAACCGAAGCCATGCTTATTTTGGCTGGCACCGTAATCAAGATGGATATGCTGTAGAAGCTGATTCTATGATGTTCTTGAATAGACACTTTCTTCCTCTTGATGATTCCACAAGTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005488 GO:0005506 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008199 GO:0008270 GO:0009267 GO:0009505 GO:0009506 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030054 GO:0030145 GO:0030312 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042578 GO:0042594 GO:0043167 GO:0043169 GO:0044237 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0055044 GO:0071496 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

477

Amino Acids

54.43

Weight (kDa)

6.55

Isoelectric Point (pI)

34.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	70 - 160	1.4e-17	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	171 - 369	5.9e-28	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	395 - 454	1.1e-18	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16430 AT2G16430
fragaria_vesca	FvH4_2g09890 FvH4_2g09920 FvH4_2g09920 FvH4_2g09920
malus_domestica	MD05G1058900.v1.1 MD05G1059100.v1.1 MD05G1059300.v1.1 MD06G1221800.v1.1 MD10G1067300.v1.1
prunus_persica	Prupe.8G088800_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G089000_v2.0.a1 Prupe.8G089100_v2.0.a1 Prupe.8G089200_v2.0.a1
pyrus_communis	pycom05g05060 pycom05g05070 pycom10g05330
rosa_chinensis	RchiOBHm_Chr5g0073481 RchiOBHm_Chr6g0266421 RchiOBHm_Chr6g0266561 RchiOBHm_Chr6g0266681
rosa_laevigata	RLG00000014085 RLG00000014091 RLG00000014096
rosa_multiflora	Rmu_sc0003605.1_g000010 Rmu_sc0006413.1_g000013 Rmu_sc0022454.1_g000004 Rmu_sc0023098.1_g000001 Rmu_ssc0000226.1_g000024
rosa_roxburghii	Rroxscaffold_1G00007780 Rroxscaffold_7G00201560 Rroxscaffold_7G00201660
rosa_rugosa	Rorug05G0423800 Rorug06G0025500 Rorug06G0026200
rosa_samantha	Rh5AG481100 Rh5BG501800 Rh5CG525000 Rh5DG514200 Rh6AG146200 Rh6AG146700 Rh6AG147400 Rh6BG146400 Rh6BG147800 Rh6CG142800 Rh6CG143600 Rh6DG131000 Rh6DG132400
rosa_wichuraiana	Rw5G044690 Rw6G012590 Rw6G012600 Rw6G012660 Rw6G012700 Rw6G012810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	504
AccB1I	GGYRCC	1 cut(s)	1338
AccBSI	CCGCTC	1 cut(s)	460
AciI	CCGC	3 cut(s)	460, 629, 888
AclWI	GGATC	1 cut(s)	537
AcoI	YGGCCR	1 cut(s)	448
AcyI	GRCGYC	1 cut(s)	501
AfaI	GTAC	7 cut(s)	295, 823, 839, 914, 1062, 1067, 1262
AfiI	CCNNNNNNNGG	5 cut(s)	54, 100, 491, 590, 755
AgsI	TTSAA	5 cut(s)	91, 418, 552, 1223, 1390
AjnI	CCWGG	4 cut(s)	93, 193, 283, 1052
AluBI	AGCT	7 cut(s)	62, 854, 878, 934, 1002, 1284, 1370
AluI	AGCT	7 cut(s)	62, 854, 878, 934, 1002, 1284, 1370
Alw26I	GTCTC	2 cut(s)	524, 950
AlwI	GGATC	1 cut(s)	537
Ama87I	CYCGRG	1 cut(s)	1256
AoxI	GGCC	4 cut(s)	448, 744, 1266, 1278
ApeKI	GCWGC	1 cut(s)	59
AspS9I	GGNCC	3 cut(s)	45, 494, 745
AsuHPI	GGTGA	4 cut(s)	280, 380, 626, 770
AvaI	CYCGRG	1 cut(s)	1256
AvaII	GGWCC	2 cut(s)	45, 494
BanI	GGYRCC	1 cut(s)	1338
BanII	GRGCYC	1 cut(s)	1053
BbvCI	CCTCAGC	1 cut(s)	336
BbvI	GCAGC	1 cut(s)	71
BccI	CCATC	4 cut(s)	4, 654, 1202, 1346
BciT130I	CCWGG	4 cut(s)	95, 195, 285, 1054
BciVI	GTATCC	1 cut(s)	658
BclI	TGATCA	1 cut(s)	643
BcoDI	GTCTC	2 cut(s)	524, 950
BfaI	CTAG	1 cut(s)	123
BfmI	CTRYAG	2 cut(s)	720, 1362
BfuI	GTATCC	1 cut(s)	658
BisI	GCNGC	2 cut(s)	60, 461
BlpI	GCTNAGC	1 cut(s)	1228
BlsI	GCNGC	2 cut(s)	61, 462
BmcAI	AGTACT	3 cut(s)	295, 823, 1262
Bme1390I	CCNGG	4 cut(s)	95, 195, 285, 1054
Bme18I	GGWCC	2 cut(s)	45, 494
BmeT110I	CYCGRG	1 cut(s)	1256
BmgT120I	GGNCC	3 cut(s)	45, 494, 745
BmiI	GGNNCC	3 cut(s)	289, 747, 1340
BmrFI	CCNGG	4 cut(s)	95, 195, 285, 1054
BmsI	GCATC	2 cut(s)	796, 821
BpmI	CTGGAG	2 cut(s)	177, 328
Bpu10I	CCTNAGC	1 cut(s)	336
Bpu1102I	GCTNAGC	1 cut(s)	1228
BpuEI	CTTGAG	1 cut(s)	944
BsaHI	GRCGYC	1 cut(s)	501
BsaI	GGTCTC	1 cut(s)	524
BsaJI	CCNNGG	5 cut(s)	94, 707, 962, 989, 1052
BsaXI	ACNNNNNCTCC	2 cut(s)	585, 615
Bsc4I	CCNNNNNNNGG	5 cut(s)	54, 100, 491, 590, 755
Bse1I	ACTGG	2 cut(s)	291, 311
BseBI	CCWGG	4 cut(s)	95, 195, 285, 1054
BseDI	CCNNGG	5 cut(s)	94, 707, 962, 989, 1052
BseGI	GGATG	5 cut(s)	15, 283, 637, 857, 1213
BseLI	CCNNNNNNNGG	5 cut(s)	54, 100, 491, 590, 755
BseMII	CTCAG	3 cut(s)	327, 447, 923
BseNI	ACTGG	2 cut(s)	291, 311
BseRI	GAGGAG	2 cut(s)	471, 897
BseXI	GCAGC	1 cut(s)	71
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	4 cut(s)	450, 746, 1268, 1280
BshNI	GGYRCC	1 cut(s)	1338
BsiEI	CGRYCG	1 cut(s)	238
BsiHKCI	CYCGRG	1 cut(s)	1256
BslFI	GGGAC	3 cut(s)	173, 488, 785
BslI	CCNNNNNNNGG	5 cut(s)	54, 100, 491, 590, 755
BsmAI	GTCTC	2 cut(s)	524, 950
BsmFI	GGGAC	3 cut(s)	173, 488, 785
BsmI	GAATGC	2 cut(s)	982, 1161
BsnI	GGCC	4 cut(s)	450, 746, 1268, 1280
Bso31I	GGTCTC	1 cut(s)	524
BsoBI	CYCGRG	1 cut(s)	1256
Bsp1286I	GDGCHC	1 cut(s)	1053
Bsp1407I	TGTACA	1 cut(s)	1060
Bsp143I	GATC	4 cut(s)	529, 643, 1112, 1174
Bsp1720I	GCTNAGC	1 cut(s)	1228
Bsp19I	CCATGG	2 cut(s)	962, 989
BspACI	CCGC	3 cut(s)	460, 629, 888
BspANI	GGCC	4 cut(s)	450, 746, 1268, 1280
BspCNI	CTCAG	3 cut(s)	328, 448, 922
BspHI	TCATGA	2 cut(s)	646, 730
BspLI	GGNNCC	3 cut(s)	289, 747, 1340
BspMAI	CTGCAG	1 cut(s)	724
BspPI	GGATC	1 cut(s)	537
BspT107I	GGYRCC	1 cut(s)	1338
BspTNI	GGTCTC	1 cut(s)	524
BsrBI	CCGCTC	1 cut(s)	460
BsrGI	TGTACA	1 cut(s)	1060
BsrI	ACTGG	2 cut(s)	291, 311
BssECI	CCNNGG	5 cut(s)	94, 707, 962, 989, 1052
BssMI	GATC	4 cut(s)	529, 643, 1112, 1174
BssNI	GRCGYC	1 cut(s)	501
BssT1I	CCWWGG	3 cut(s)	707, 962, 989
Bst2UI	CCWGG	4 cut(s)	95, 195, 285, 1054
Bst4CI	ACNGT	6 cut(s)	173, 239, 274, 298, 1065, 1343
BstACI	GRCGYC	1 cut(s)	501
BstAUI	TGTACA	1 cut(s)	1060
BstC8I	GCNNGC	2 cut(s)	1282, 1337
BstDEI	CTNAG	5 cut(s)	336, 456, 874, 909, 1228
BstDSI	CCRYGG	2 cut(s)	962, 989
BstEII	GGTNACC	1 cut(s)	614
BstF5I	GGATG	5 cut(s)	15, 283, 637, 857, 1213
BstKTI	GATC	4 cut(s)	532, 646, 1115, 1177
BstMAI	GTCTC	2 cut(s)	524, 950
BstMBI	GATC	4 cut(s)	529, 643, 1112, 1174
BstMCI	CGRYCG	1 cut(s)	238
BstMWI	GCNNNNNNNGC	3 cut(s)	123, 784, 1367
BstNI	CCWGG	4 cut(s)	95, 195, 285, 1054
BstPI	GGTNACC	1 cut(s)	614
BstSCI	CCNGG	4 cut(s)	93, 193, 283, 1052
BstSFI	CTRYAG	2 cut(s)	720, 1362
BstV1I	GCAGC	1 cut(s)	71
BstX2I	RGATCY	1 cut(s)	529
BstYI	RGATCY	1 cut(s)	529
BsuI	GTATCC	1 cut(s)	658
BsuRI	GGCC	4 cut(s)	450, 746, 1268, 1280
BtgI	CCRYGG	2 cut(s)	962, 989
BtsCI	GGATG	5 cut(s)	15, 283, 637, 857, 1213
BtsIMutI	CAGTG	2 cut(s)	178, 453
Cac8I	GCNNGC	2 cut(s)	1282, 1337
CciI	TCATGA	2 cut(s)	646, 730
Cfr13I	GGNCC	3 cut(s)	45, 494, 745
CsiI	ACCWGGT	1 cut(s)	283
Csp6I	GTAC	7 cut(s)	294, 822, 838, 913, 1061, 1066, 1261
CviQI	GTAC	7 cut(s)	294, 822, 838, 913, 1061, 1066, 1261
DdeI	CTNAG	5 cut(s)	336, 456, 874, 909, 1228
DpnI	GATC	4 cut(s)	531, 645, 1114, 1176
DpnII	GATC	4 cut(s)	529, 643, 1112, 1174
DraI	TTTAAA	1 cut(s)	184
EaeI	YGGCCR	1 cut(s)	448
Eco130I	CCWWGG	3 cut(s)	707, 962, 989
Eco24I	GRGCYC	1 cut(s)	1053
Eco31I	GGTCTC	1 cut(s)	524
Eco47I	GGWCC	2 cut(s)	45, 494
Eco88I	CYCGRG	1 cut(s)	1256
Eco91I	GGTNACC	1 cut(s)	614
EcoO65I	GGTNACC	1 cut(s)	614
EcoRII	CCWGG	4 cut(s)	93, 193, 283, 1052
EcoT14I	CCWWGG	3 cut(s)	707, 962, 989
EcoT22I	ATGCAT	1 cut(s)	984
EcoT38I	GRGCYC	1 cut(s)	1053
ErhI	CCWWGG	3 cut(s)	707, 962, 989
FalI	AAGNNNNNCTT	4 cut(s)	682, 714, 1309, 1341
FaqI	GGGAC	3 cut(s)	173, 488, 785
FauNDI	CATATG	1 cut(s)	892
FbaI	TGATCA	1 cut(s)	643
Fnu4HI	GCNGC	2 cut(s)	60, 461
FokI	GGATG	5 cut(s)	22, 290, 644, 844, 1220
FriOI	GRGCYC	1 cut(s)	1053
Fsp4HI	GCNGC	2 cut(s)	60, 461
FspBI	CTAG	1 cut(s)	123
GluI	GCNGC	2 cut(s)	60, 461
GsuI	CTGGAG	2 cut(s)	177, 328
HaeIII	GGCC	4 cut(s)	450, 746, 1268, 1280
Hin1I	GRCGYC	1 cut(s)	501
HinfI	GANTC	6 cut(s)	444, 548, 678, 727, 1373, 1415
HphI	GGTGA	4 cut(s)	280, 380, 626, 770
Hpy188I	TCNGA	3 cut(s)	540, 659, 1117
HpyAV	CCTTC	5 cut(s)	241, 325, 584, 1016, 1217
HpyCH4III	ACNGT	6 cut(s)	173, 239, 274, 298, 1065, 1343
HpyCH4IV	ACGT	1 cut(s)	501
HpyCH4V	TGCA	6 cut(s)	206, 314, 722, 982, 1136, 1161
HpyF10VI	GCNNNNNNNGC	3 cut(s)	123, 784, 1367
HpyF3I	CTNAG	5 cut(s)	336, 456, 874, 909, 1228
HpySE526I	ACGT	1 cut(s)	501
Hsp92I	GRCGYC	1 cut(s)	501
Ksp22I	TGATCA	1 cut(s)	643
Kzo9I	GATC	4 cut(s)	529, 643, 1112, 1174
LmnI	GCTCC	2 cut(s)	832, 1189
Lsp1109I	GCAGC	1 cut(s)	71
LweI	GCATC	2 cut(s)	796, 821
MabI	ACCWGGT	1 cut(s)	283
MaeI	CTAG	1 cut(s)	123
MaeII	ACGT	1 cut(s)	501
MaeIII	GTNAC	4 cut(s)	268, 380, 475, 614
MalI	GATC	4 cut(s)	531, 645, 1114, 1176
MbiI	CCGCTC	1 cut(s)	460
MboI	GATC	4 cut(s)	529, 643, 1112, 1174
MboII	GAAGA	3 cut(s)	16, 687, 1394
MflI	RGATCY	1 cut(s)	529
MhlI	GDGCHC	1 cut(s)	1053
MluCI	AATT	6 cut(s)	141, 367, 735, 753, 969, 1156
MmeI	TCCRAC	1 cut(s)	421
Mph1103I	ATGCAT	1 cut(s)	984
MseI	TTAA	6 cut(s)	183, 348, 774, 945, 968, 977
MslI	CAYNNNNRTG	5 cut(s)	651, 1037, 1098, 1104, 1166
MspR9I	CCNGG	4 cut(s)	95, 195, 285, 1054
Mva1269I	GAATGC	2 cut(s)	982, 1161
MvaI	CCWGG	4 cut(s)	95, 195, 285, 1054
MwoI	GCNNNNNNNGC	3 cut(s)	123, 784, 1367
NcoI	CCATGG	2 cut(s)	962, 989
NdeI	CATATG	1 cut(s)	892
NdeII	GATC	4 cut(s)	529, 643, 1112, 1174
NlaIV	GGNNCC	3 cut(s)	289, 747, 1340
NmeAIII	GCCGAG	1 cut(s)	1244
NmuCI	GTSAC	2 cut(s)	268, 614
NsiI	ATGCAT	1 cut(s)	984
PagI	TCATGA	2 cut(s)	646, 730
PctI	GAATGC	2 cut(s)	982, 1161
PfeI	GAWTC	6 cut(s)	444, 548, 678, 727, 1373, 1415
PkrI	GCNGC	2 cut(s)	61, 462
Psp6I	CCWGG	4 cut(s)	93, 193, 283, 1052
PspEI	GGTNACC	1 cut(s)	614
PspGI	CCWGG	4 cut(s)	93, 193, 283, 1052
PspN4I	GGNNCC	3 cut(s)	289, 747, 1340
PspPI	GGNCC	3 cut(s)	45, 494, 745
PstI	CTGCAG	1 cut(s)	724
PsuI	RGATCY	1 cut(s)	529
RsaI	GTAC	7 cut(s)	295, 823, 839, 914, 1062, 1067, 1262
RsaNI	GTAC	7 cut(s)	294, 822, 838, 913, 1061, 1066, 1261
RseI	CAYNNNNRTG	5 cut(s)	651, 1037, 1098, 1104, 1166
SaqAI	TTAA	6 cut(s)	183, 348, 774, 945, 968, 977
SatI	GCNGC	2 cut(s)	60, 461
Sau3AI	GATC	4 cut(s)	529, 643, 1112, 1174
Sau96I	GGNCC	3 cut(s)	45, 494, 745
ScaI	AGTACT	3 cut(s)	295, 823, 1262
ScrFI	CCNGG	4 cut(s)	95, 195, 285, 1054
SduI	GDGCHC	1 cut(s)	1053
SexAI	ACCWGGT	1 cut(s)	283
SfaNI	GCATC	2 cut(s)	796, 821
SfcI	CTRYAG	2 cut(s)	720, 1362
SinI	GGWCC	2 cut(s)	45, 494
SmiMI	CAYNNNNRTG	5 cut(s)	651, 1037, 1098, 1104, 1166
SmlI	CTYRAG	1 cut(s)	923
SmoI	CTYRAG	1 cut(s)	923
Sse9I	AATT	6 cut(s)	141, 367, 735, 753, 969, 1156
SsiI	CCGC	3 cut(s)	460, 629, 888
SspI	AATATT	1 cut(s)	428
SspMI	CTAG	1 cut(s)	123
StyD4I	CCNGG	4 cut(s)	93, 193, 283, 1052
StyI	CCWWGG	3 cut(s)	707, 962, 989
TaaI	ACNGT	6 cut(s)	173, 239, 274, 298, 1065, 1343
TaiI	ACGT	1 cut(s)	504
TaqI	TCGA	2 cut(s)	1074, 1273
TasI	AATT	6 cut(s)	141, 367, 735, 753, 969, 1156
TatI	WGTACW	5 cut(s)	293, 821, 1060, 1065, 1260
TauI	GCSGC	1 cut(s)	463
TfiI	GAWTC	6 cut(s)	444, 548, 678, 727, 1373, 1415
Tru1I	TTAA	6 cut(s)	183, 348, 774, 945, 968, 977
Tru9I	TTAA	6 cut(s)	183, 348, 774, 945, 968, 977
TscAI	CASTG	2 cut(s)	178, 460
TseFI	GTSAC	2 cut(s)	268, 614
TseI	GCWGC	1 cut(s)	59
Tsp45I	GTSAC	2 cut(s)	268, 614
TspDTI	ATGAA	8 cut(s)	17, 209, 294, 344, 747, 846, 870, 1122
TspRI	CASTG	2 cut(s)	178, 460
VpaK11BI	GGWCC	2 cut(s)	45, 494
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1328
XspI	CTAG	1 cut(s)	123
ZraI	GACGTC	1 cut(s)	502
ZrmI	AGTACT	3 cut(s)	295, 823, 1262
Zsp2I	ATGCAT	1 cut(s)	984

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.