Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000988

Physical Location & Seq

Genomic Coordinates Forward (+)

10350920 .. 10354674

Gene Structure 3755 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1059300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGGGTGTGCTGGATTTAGCTTCTTCAAGGGCAGCAGCAGCCGCTGTTGTAGTGCTGGGTTTGGTCTTGAGCTTTGGAGTGGTGTGCAATGGAGGCAAAACAAGTAGTTTTGTGAGGAAAGTTGAGAAGACTATGGACATGCCTCTTGACAGTGATGTCTTCAAAGTCCCTCCTGGCTATAATGCACCGCAACAAGTTCATATAACACAAGGAGACCAACTGGGAAAGGCAGTGATCGTTTCATGGGTTACTGTGGATGAACCCGGTTCCAGTAGTGTGCTTTACTGGAGTGCAAACAGCAAGAAAAACAAGGCCGAGGGCAAAATTACAACCTATAAATTCTACAATTACACTTCCGGTTACATTCACCACACCACTGTCAGAAATTTAATGTTCAATACCAAATACTACTATGTGATTGGAATTGGCCATACCGAGCGGCAGTTCTGGTTTATAACTCCTCCTGAAGTTGGTCCGGACACCTCTTACACATTTGGACTCATAGGGGATCTGGGACAGAGCTATGATTCAAATAAGACGCTTACTCATTACGAGTTAAACCCGCAGAAAGGACAAGCAGTATTGTTTGTTGGGGACCTCTCTTATGCAGATAACTACCCAAATCATGACAATGTCAGGTGGGATACTTGGGGAAGATTCGTCGAGAGAAGTGTTGCTTATCAACCTTGGATATGGACTGCAGGAAACCATGAAATCGATTTTGCCCCAGAAATCGGTGAAACCAAACCTTTTAAGCCTTACAGTCACCGGTATCATGTCCCCTATAAAGCATCTGGGAGTACTTCCCCCTTTTGGTATTCGATCAAGAGAGCTTCGGCATACATCATAGTCTTGTCTTCTTATTCGGCATATGGTAAATACACCCCTCAGTACAAATGGCTCGAGGAGGAACTACCGAAGGTTAATAGGAGTGAAACACCATGGTTGATTGTTTTAATGCATTCTCCATGGTACAACAGCTACAACTATCACTATATGGAAGGAGAAACCATGAGAGTGATGTATGAGCCATGGTTTGTGAAGTACAAAGTCGACGTGGTATTTGCTGGTCATGTCCATGCATATGAACGATCTGAACGTGTATCCAATGTTGCATACAACATCGTAAATGGCATTTGCAAACCTGTGAAAGATCAATATGCACCAGTGTACATTACGATTGGTGATGGAGGAAATCTTGAAGGCTTAGCGACAAATATGACAGAACCACAGCCAGATTACTCAGCTTATAGAGAGGCCAGTTTTGGTCATGCTACTTTAGACATCAAGAATCGAACCCATGCCTACTATAGTTGGCACCGTAATCAAGATGGATATGCAGTAAAAGCAGATTCTATGTGGTTTTTCAATAGATTCTATCACCCTGTAGATGATTCCACAACTGCTGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005488 GO:0005506 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008199 GO:0008270 GO:0009267 GO:0009505 GO:0009506 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030054 GO:0030145 GO:0030312 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042578 GO:0042594 GO:0043167 GO:0043169 GO:0044237 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0055044 GO:0071496 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

471

Amino Acids

53.7

Weight (kDa)

6.63

Isoelectric Point (pI)

37.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	63 - 151	4.7e-17	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	164 - 362	1e-29	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	388 - 447	1e-20	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16430 AT2G16430
fragaria_vesca	FvH4_2g09890 FvH4_2g09920 FvH4_2g09920 FvH4_2g09920
malus_domestica	MD05G1058900.v1.1 MD05G1059100.v1.1 MD05G1059300.v1.1 MD06G1221800.v1.1 MD10G1067300.v1.1
prunus_persica	Prupe.8G088800_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G089000_v2.0.a1 Prupe.8G089100_v2.0.a1 Prupe.8G089200_v2.0.a1
pyrus_communis	pycom05g05060 pycom05g05070 pycom10g05330
rosa_chinensis	RchiOBHm_Chr5g0073481 RchiOBHm_Chr6g0266421 RchiOBHm_Chr6g0266561 RchiOBHm_Chr6g0266681
rosa_laevigata	RLG00000014085 RLG00000014091 RLG00000014096
rosa_multiflora	Rmu_sc0003605.1_g000010 Rmu_sc0006413.1_g000013 Rmu_sc0022454.1_g000004 Rmu_sc0023098.1_g000001 Rmu_ssc0000226.1_g000024
rosa_roxburghii	Rroxscaffold_1G00007780 Rroxscaffold_7G00201560 Rroxscaffold_7G00201660
rosa_rugosa	Rorug05G0423800 Rorug06G0025500 Rorug06G0026200
rosa_samantha	Rh5AG481100 Rh5BG501800 Rh5CG525000 Rh5DG514200 Rh6AG146200 Rh6AG146700 Rh6AG147400 Rh6BG146400 Rh6BG147800 Rh6CG142800 Rh6CG143600 Rh6DG131000 Rh6DG132400
rosa_wichuraiana	Rw5G044690 Rw6G012590 Rw6G012600 Rw6G012660 Rw6G012700 Rw6G012810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	455
AasI	GACNNNNNNGTC	1 cut(s)	155
AccB1I	GGYRCC	1 cut(s)	1317
AccBSI	CCGCTC	1 cut(s)	439
AccI	GTMKAC	1 cut(s)	1053
AccIII	TCCGGA	1 cut(s)	475
AciI	CCGC	4 cut(s)	42, 188, 439, 563
AclWI	GGATC	1 cut(s)	516
AcoI	YGGCCR	1 cut(s)	427
AcsI	RAATTY	2 cut(s)	338, 385
AcuI	CTGAAG	1 cut(s)	486
AfaI	GTAC	5 cut(s)	802, 893, 974, 1046, 1172
AfiI	CCNNNNNNNGG	4 cut(s)	470, 569, 734, 813
AflIII	ACRYGT	1 cut(s)	1099
AgeI	ACCGGT	1 cut(s)	768
AgsI	TTSAA	6 cut(s)	27, 163, 397, 531, 1202, 1369
AjiI	CACGTC	1 cut(s)	1057
AjnI	CCWGG	1 cut(s)	172
AluBI	AGCT	6 cut(s)	20, 72, 522, 833, 981, 1247
AluI	AGCT	6 cut(s)	20, 72, 522, 833, 981, 1247
Alw26I	GTCTC	1 cut(s)	207
AlwI	GGATC	1 cut(s)	516
AlwNI	CAGNNNCTG	1 cut(s)	44
Ama87I	CYCGRG	1 cut(s)	902
Aor13HI	TCCGGA	1 cut(s)	475
AoxI	GGCC	3 cut(s)	312, 427, 1257
ApeKI	GCWGC	3 cut(s)	32, 35, 38
ApoI	RAATTY	2 cut(s)	338, 385
ArsI	GACNNNNNNTTYG	2 cut(s)	1046, 1078
AsiGI	ACCGGT	1 cut(s)	768
AspS9I	GGNCC	2 cut(s)	473, 595
AsuC2I	CCSGG	1 cut(s)	264
AsuHPI	GGTGA	5 cut(s)	359, 749, 758, 1196, 1373
AvaI	CYCGRG	1 cut(s)	902
AvaII	GGWCC	2 cut(s)	473, 595
BalI	TGGCCA	1 cut(s)	429
BanI	GGYRCC	1 cut(s)	1317
BbsI	GAAGAC	3 cut(s)	134, 151, 849
BbvI	GCAGC	3 cut(s)	44, 47, 50
BccI	CCATC	2 cut(s)	1181, 1325
BciT130I	CCWGG	1 cut(s)	174
BciVI	GTATCC	2 cut(s)	637, 1114
BcnI	CCSGG	1 cut(s)	264
BcoDI	GTCTC	1 cut(s)	207
BfmI	CTRYAG	3 cut(s)	699, 1309, 1386
BfuI	GTATCC	2 cut(s)	637, 1114
BisI	GCNGC	5 cut(s)	33, 36, 39, 42, 440
BlpI	GCTNAGC	1 cut(s)	1207
BlsI	GCNGC	5 cut(s)	34, 37, 40, 43, 441
BmcAI	AGTACT	1 cut(s)	802
Bme1390I	CCNGG	2 cut(s)	174, 264
Bme18I	GGWCC	2 cut(s)	473, 595
BmeT110I	CYCGRG	1 cut(s)	902
BmgBI	CACGTC	1 cut(s)	1057
BmgT120I	GGNCC	2 cut(s)	473, 595
BmiI	GGNNCC	3 cut(s)	268, 596, 1319
BmrFI	CCNGG	2 cut(s)	174, 264
BmrI	ACTGGG	1 cut(s)	230
BmsI	GCATC	1 cut(s)	800
BmuI	ACTGGG	1 cut(s)	230
BpiI	GAAGAC	3 cut(s)	134, 151, 849
BpmI	CTGGAG	1 cut(s)	307
Bpu1102I	GCTNAGC	1 cut(s)	1207
BpuEI	CTTGAG	1 cut(s)	88
BpuMI	CCSGG	1 cut(s)	264
Bsa29I	ATCGAT	1 cut(s)	717
BsaI	GGTCTC	1 cut(s)	207
BsaJI	CCNNGG	5 cut(s)	315, 686, 941, 968, 1031
BsaWI	WCCGGW	3 cut(s)	356, 475, 768
Bsc4I	CCNNNNNNNGG	4 cut(s)	470, 569, 734, 813
Bse118I	RCCGGY	1 cut(s)	768
Bse1I	ACTGG	5 cut(s)	225, 270, 290, 1166, 1260
Bse3DI	GCAATG	1 cut(s)	94
BseAI	TCCGGA	1 cut(s)	475
BseBI	CCWGG	1 cut(s)	174
BseCI	ATCGAT	1 cut(s)	717
BseDI	CCNNGG	5 cut(s)	315, 686, 941, 968, 1031
BseGI	GGATG	1 cut(s)	262
BseLI	CCNNNNNNNGG	4 cut(s)	470, 569, 734, 813
BseMI	GCAATG	1 cut(s)	94
BseMII	CTCAG	2 cut(s)	902, 1257
BseNI	ACTGG	5 cut(s)	225, 270, 290, 1166, 1260
BseRI	GAGGAG	2 cut(s)	450, 920
BseXI	GCAGC	3 cut(s)	44, 47, 50
BseYI	CCCAGC	1 cut(s)	55
BshFI	GGCC	3 cut(s)	314, 429, 1259
BshNI	GGYRCC	1 cut(s)	1317
BshTI	ACCGGT	1 cut(s)	768
BshVI	ATCGAT	1 cut(s)	717
BsiHKCI	CYCGRG	1 cut(s)	902
BsiSI	CCGG	4 cut(s)	264, 357, 476, 769
BslFI	GGGAC	4 cut(s)	152, 528, 608, 764
BslI	CCNNNNNNNGG	4 cut(s)	470, 569, 734, 813
BsmAI	GTCTC	1 cut(s)	207
BsmFI	GGGAC	4 cut(s)	152, 528, 608, 764
BsmI	GAATGC	1 cut(s)	961
BsnI	GGCC	3 cut(s)	314, 429, 1259
Bso31I	GGTCTC	1 cut(s)	207
BsoBI	CYCGRG	1 cut(s)	902
Bsp13I	TCCGGA	1 cut(s)	475
Bsp1407I	TGTACA	1 cut(s)	1170
Bsp143I	GATC	5 cut(s)	234, 508, 822, 1091, 1153
Bsp1720I	GCTNAGC	1 cut(s)	1207
Bsp19I	CCATGG	3 cut(s)	941, 968, 1031
BspACI	CCGC	4 cut(s)	42, 188, 439, 563
BspANI	GGCC	3 cut(s)	314, 429, 1259
BspCNI	CTCAG	2 cut(s)	901, 1256
BspDI	ATCGAT	1 cut(s)	717
BspEI	TCCGGA	1 cut(s)	475
BspHI	TCATGA	1 cut(s)	625
BspLI	GGNNCC	3 cut(s)	268, 596, 1319
BspMAI	CTGCAG	1 cut(s)	703
BspPI	GGATC	1 cut(s)	516
BspT107I	GGYRCC	1 cut(s)	1317
BspTNI	GGTCTC	1 cut(s)	207
BsrBI	CCGCTC	1 cut(s)	439
BsrDI	GCAATG	1 cut(s)	94
BsrFI	RCCGGY	1 cut(s)	768
BsrGI	TGTACA	1 cut(s)	1170
BsrI	ACTGG	5 cut(s)	225, 270, 290, 1166, 1260
BssAI	RCCGGY	1 cut(s)	768
BssECI	CCNNGG	5 cut(s)	315, 686, 941, 968, 1031
BssMI	GATC	5 cut(s)	234, 508, 822, 1091, 1153
BssT1I	CCWWGG	4 cut(s)	686, 941, 968, 1031
Bst2UI	CCWGG	1 cut(s)	174
Bst4CI	ACNGT	5 cut(s)	152, 253, 379, 764, 1322
BstAUI	TGTACA	1 cut(s)	1170
BstDEI	CTNAG	3 cut(s)	888, 1207, 1243
BstDSI	CCRYGG	3 cut(s)	941, 968, 1031
BstF5I	GGATG	1 cut(s)	262
BstKTI	GATC	5 cut(s)	237, 511, 825, 1094, 1156
BstMAI	GTCTC	1 cut(s)	207
BstMBI	GATC	5 cut(s)	234, 508, 822, 1091, 1153
BstMWI	GCNNNNNNNGC	4 cut(s)	38, 41, 93, 1346
BstNI	CCWGG	1 cut(s)	174
BstNSI	RCATGY	1 cut(s)	142
BstSCI	CCNGG	2 cut(s)	172, 262
BstSFI	CTRYAG	3 cut(s)	699, 1309, 1386
BstV1I	GCAGC	3 cut(s)	44, 47, 50
BstV2I	GAAGAC	3 cut(s)	134, 151, 849
BstX2I	RGATCY	1 cut(s)	508
BstYI	RGATCY	1 cut(s)	508
Bsu15I	ATCGAT	1 cut(s)	717
BsuI	GTATCC	2 cut(s)	637, 1114
BsuRI	GGCC	3 cut(s)	314, 429, 1259
BsuTUI	ATCGAT	1 cut(s)	717
BtgI	CCRYGG	3 cut(s)	941, 968, 1031
BtrI	CACGTC	1 cut(s)	1057
BtsCI	GGATG	1 cut(s)	262
BtsI	GCAGTG	1 cut(s)	237
BtsIMutI	CAGTG	4 cut(s)	157, 237, 375, 1173
CaiI	CAGNNNCTG	1 cut(s)	44
CciI	TCATGA	1 cut(s)	625
Cfr10I	RCCGGY	1 cut(s)	768
Cfr13I	GGNCC	2 cut(s)	473, 595
ClaI	ATCGAT	1 cut(s)	717
CseI	GACGC	1 cut(s)	547
Csp6I	GTAC	5 cut(s)	801, 892, 973, 1045, 1171
CspAI	ACCGGT	1 cut(s)	768
CviQI	GTAC	5 cut(s)	801, 892, 973, 1045, 1171
DdeI	CTNAG	3 cut(s)	888, 1207, 1243
DpnI	GATC	5 cut(s)	236, 510, 824, 1093, 1155
DpnII	GATC	5 cut(s)	234, 508, 822, 1091, 1153
DrdI	GACNNNNNNGTC	1 cut(s)	155
DseDI	GACNNNNNNGTC	1 cut(s)	155
EaeI	YGGCCR	1 cut(s)	427
Eco130I	CCWWGG	4 cut(s)	686, 941, 968, 1031
Eco31I	GGTCTC	1 cut(s)	207
Eco47I	GGWCC	2 cut(s)	473, 595
Eco57I	CTGAAG	1 cut(s)	486
Eco88I	CYCGRG	1 cut(s)	902
EcoO109I	RGGNCCY	1 cut(s)	595
EcoRII	CCWGG	1 cut(s)	172
EcoT14I	CCWWGG	4 cut(s)	686, 941, 968, 1031
EcoT22I	ATGCAT	2 cut(s)	963, 1084
ErhI	CCWWGG	4 cut(s)	686, 941, 968, 1031
FalI	AAGNNNNNCTT	2 cut(s)	661, 693
FaqI	GGGAC	4 cut(s)	152, 528, 608, 764
FauI	CCCGC	1 cut(s)	570
FauNDI	CATATG	2 cut(s)	871, 1084
FblI	GTMKAC	1 cut(s)	1053
Fnu4HI	GCNGC	5 cut(s)	33, 36, 39, 42, 440
FokI	GGATG	1 cut(s)	269
Fsp4HI	GCNGC	5 cut(s)	33, 36, 39, 42, 440
GluI	GCNGC	5 cut(s)	33, 36, 39, 42, 440
GsaI	CCCAGC	1 cut(s)	59
GsuI	CTGGAG	1 cut(s)	307
HaeIII	GGCC	3 cut(s)	314, 429, 1259
HapII	CCGG	4 cut(s)	264, 357, 476, 769
HgaI	GACGC	1 cut(s)	547
HincII	GTYRAC	1 cut(s)	1054
HindII	GTYRAC	1 cut(s)	1054
HinfI	GANTC	7 cut(s)	498, 527, 657, 1291, 1352, 1374, 1394
HpaII	CCGG	4 cut(s)	264, 357, 476, 769
HphI	GGTGA	5 cut(s)	359, 749, 758, 1196, 1373
Hpy166II	GTNNAC	2 cut(s)	1054, 1171
Hpy188I	TCNGA	2 cut(s)	383, 1096
Hpy8I	GTNNAC	2 cut(s)	1054, 1171
Hpy99I	CGWCG	2 cut(s)	665, 1058
HpyAV	CCTTC	3 cut(s)	913, 995, 1196
HpyCH4III	ACNGT	5 cut(s)	152, 253, 379, 764, 1322
HpyCH4IV	ACGT	2 cut(s)	1056, 1099
HpyF10VI	GCNNNNNNNGC	4 cut(s)	38, 41, 93, 1346
HpyF3I	CTNAG	3 cut(s)	888, 1207, 1243
HpySE526I	ACGT	2 cut(s)	1056, 1099
Kpn2I	TCCGGA	1 cut(s)	475
Kzo9I	GATC	5 cut(s)	234, 508, 822, 1091, 1153
Lsp1109I	GCAGC	3 cut(s)	44, 47, 50
LweI	GCATC	1 cut(s)	800
MaeII	ACGT	2 cut(s)	1056, 1099
MaeIII	GTNAC	3 cut(s)	247, 359, 764
MalI	GATC	5 cut(s)	236, 510, 824, 1093, 1155
MbiI	CCGCTC	1 cut(s)	439
MboI	GATC	5 cut(s)	234, 508, 822, 1091, 1153
MboII	GAAGA	5 cut(s)	15, 139, 151, 666, 849
MflI	RGATCY	1 cut(s)	508
MlsI	TGGCCA	1 cut(s)	429
MluCI	AATT	5 cut(s)	324, 338, 346, 385, 423
MluNI	TGGCCA	1 cut(s)	429
MlyI	GAGTC	1 cut(s)	492
Mox20I	TGGCCA	1 cut(s)	429
Mph1103I	ATGCAT	2 cut(s)	963, 1084
MroI	TCCGGA	1 cut(s)	475
MscI	TGGCCA	1 cut(s)	429
MseI	TTAA	5 cut(s)	389, 557, 753, 924, 956
MslI	CAYNNNNRTG	4 cut(s)	630, 1016, 1077, 1083
Msp20I	TGGCCA	1 cut(s)	429
MspA1I	CMGCKG	1 cut(s)	44
MspI	CCGG	4 cut(s)	264, 357, 476, 769
MspR9I	CCNGG	2 cut(s)	174, 264
Mva1269I	GAATGC	1 cut(s)	961
MvaI	CCWGG	1 cut(s)	174
MwoI	GCNNNNNNNGC	4 cut(s)	38, 41, 93, 1346
NciI	CCSGG	1 cut(s)	264
NcoI	CCATGG	3 cut(s)	941, 968, 1031
NdeI	CATATG	2 cut(s)	871, 1084
NdeII	GATC	5 cut(s)	234, 508, 822, 1091, 1153
NlaIV	GGNNCC	3 cut(s)	268, 596, 1319
NmeAIII	GCCGAG	1 cut(s)	340
NmuCI	GTSAC	1 cut(s)	764
NsiI	ATGCAT	2 cut(s)	963, 1084
NspI	RCATGY	1 cut(s)	142
PaeR7I	CTCGAG	1 cut(s)	902
PagI	TCATGA	1 cut(s)	625
PcsI	WCGNNNNNNNCGW	1 cut(s)	1096
PctI	GAATGC	1 cut(s)	961
PfeI	GAWTC	6 cut(s)	527, 657, 1291, 1352, 1374, 1394
PflFI	GACNNNGTC	1 cut(s)	632
PinAI	ACCGGT	1 cut(s)	768
PkrI	GCNGC	5 cut(s)	34, 37, 40, 43, 441
PleI	GAGTC	1 cut(s)	492
PpsI	GAGTC	1 cut(s)	492
PpuMI	RGGWCCY	1 cut(s)	595
PsiI	TTATAA	1 cut(s)	455
Psp5II	RGGWCCY	1 cut(s)	595
Psp6I	CCWGG	1 cut(s)	172
PspFI	CCCAGC	1 cut(s)	55
PspGI	CCWGG	1 cut(s)	172
PspN4I	GGNNCC	3 cut(s)	268, 596, 1319
PspPI	GGNCC	2 cut(s)	473, 595
PspPPI	RGGWCCY	1 cut(s)	595
PspXI	VCTCGAGB	1 cut(s)	902
PstI	CTGCAG	1 cut(s)	703
PstNI	CAGNNNCTG	1 cut(s)	44
PsuI	RGATCY	1 cut(s)	508
PsyI	GACNNNGTC	1 cut(s)	632
RsaI	GTAC	5 cut(s)	802, 893, 974, 1046, 1172
RsaNI	GTAC	5 cut(s)	801, 892, 973, 1045, 1171
RseI	CAYNNNNRTG	4 cut(s)	630, 1016, 1077, 1083
SalI	GTCGAC	1 cut(s)	1052
SaqAI	TTAA	5 cut(s)	389, 557, 753, 924, 956
SatI	GCNGC	5 cut(s)	33, 36, 39, 42, 440
Sau3AI	GATC	5 cut(s)	234, 508, 822, 1091, 1153
Sau96I	GGNCC	2 cut(s)	473, 595
ScaI	AGTACT	1 cut(s)	802
SchI	GAGTC	1 cut(s)	492
ScrFI	CCNGG	2 cut(s)	174, 264
SfaNI	GCATC	1 cut(s)	800
SfcI	CTRYAG	3 cut(s)	699, 1309, 1386
Sfr274I	CTCGAG	1 cut(s)	902
SinI	GGWCC	2 cut(s)	473, 595
SlaI	CTCGAG	1 cut(s)	902
SmiMI	CAYNNNNRTG	4 cut(s)	630, 1016, 1077, 1083
SmlI	CTYRAG	2 cut(s)	67, 902
SmoI	CTYRAG	2 cut(s)	67, 902
Sse9I	AATT	5 cut(s)	324, 338, 346, 385, 423
SsiI	CCGC	4 cut(s)	42, 188, 439, 563
StyD4I	CCNGG	2 cut(s)	172, 262
StyI	CCWWGG	4 cut(s)	686, 941, 968, 1031
TaaI	ACNGT	5 cut(s)	152, 253, 379, 764, 1322
TaiI	ACGT	2 cut(s)	1059, 1102
TaqI	TCGA	6 cut(s)	663, 717, 821, 903, 1053, 1294
TasI	AATT	5 cut(s)	324, 338, 346, 385, 423
TatI	WGTACW	4 cut(s)	800, 891, 1044, 1170
TauI	GCSGC	2 cut(s)	44, 442
TfiI	GAWTC	6 cut(s)	527, 657, 1291, 1352, 1374, 1394
Tru1I	TTAA	5 cut(s)	389, 557, 753, 924, 956
Tru9I	TTAA	5 cut(s)	389, 557, 753, 924, 956
TscAI	CASTG	4 cut(s)	157, 237, 382, 1173
TseFI	GTSAC	1 cut(s)	764
TseI	GCWGC	3 cut(s)	32, 35, 38
Tsp45I	GTSAC	1 cut(s)	764
TspDTI	ATGAA	5 cut(s)	188, 231, 273, 726, 1101
TspRI	CASTG	4 cut(s)	157, 237, 382, 1173
Tth111I	GACNNNGTC	1 cut(s)	632
VpaK11BI	GGWCC	2 cut(s)	473, 595
XapI	RAATTY	2 cut(s)	338, 385
XceI	RCATGY	1 cut(s)	142
XhoI	CTCGAG	1 cut(s)	902
XmiI	GTMKAC	1 cut(s)	1053
ZrmI	AGTACT	1 cut(s)	802
Zsp2I	ATGCAT	2 cut(s)	963, 1084

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.