Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000988

Physical Location & Seq

Genomic Coordinates Forward (+)

10313406 .. 10316003

Gene Structure 2598 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1058900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGTGGAAGATGGCGGTTCTTGTAGTATTTGGTCTGCTATTGAGCTTGGCAATGGTGTGTAATGGAGGTATTACAAGCACTTTTGTGAGGAAAACTGAGAGTACTGTCGATATGCCTTATGAAAGTGATGCCTTCCAAATTCCTCCAGGCCACAATGCACCTCAACAGGTTCATATAACACAAGGAGATCAAGAAGGGACGGCAATGATTGTGTCATGGATCACTCCGGATGAACCAGGTTCTAGCGTAGTGCTTTTTTGGAGCGATAAGCATAAGACAAAGAAGGCAGAGGGCAAAGCCAAACAATATAAATACCATGATTATACTTCTGGTTACATTCACCACTGCACCATCAGAAAATTAGAGCACAATACCAAATATTACTACAAGATTGGAATTGGCCAAGCTGAGAGGACATTTTGGTTTGTAACTCCTCCTGCAGTTGGGCCAGATGCCCCTTACACATTTGGTCTTCTTGGGGATTTAGGTCAAACCTTTGATTCAAACAAGACACTTGCTCATTACGAATCAAACCCATTGAAAGGCCAAACAGTTTTGTGTCTTGGTGACCTATCTTATGCTGATAACCATCCAAATCATGACCAAGTTAAGTGGGATACGTTTGGAAGGTTCATTGAGAGAAGTGCTGCTTATCAACCTTGGATTCATACTGCAGGAAATCATGAGATTGATTATGCACCAGAAATTGGTGAAACAAAACCTTTCAAGCCTTACAAGCACCGGTATACCACTCCGTTTAAAGCATCGGGGAGTACTGCTCCTTTCTGGTACTCAGTCAAGATAGCTTCAGCACACATCATAGTCTTGTCTTCATACTCATCATACGGTAAATACACTCCTCAGTATAAATGGCTGGAGCAGGAGCTACCAAAAGTTAACCGGGATGAGACACCATGGCTGATTGTTTTAGTGCATTCCCCATGGTACAATAGCTACAACTATCACTATATGGAAGGGGAAACCATGAGAGTCCAATTCGAGTCCTGGTTTGTTCAGCACAAGGTCGATGTGGTATTTGCTGGACATGTCCATGCCTACGAACGATCTGAGCGTGTATCAAATATTGCAAATAACCTCGAAAATGGTAAATGTTCCCCGGTGAAAGATCAATCTGCACCTGTCTACATTACAATTGGCGATGGAGGAAACATTGAAGGCTTAGCAACCAATATGAGAGACCCACAACCAGATTACTCAGCTTACAGAGAGGCCAGTTTTGGTCATGCCATTTTTGACATCAAGAACCGAACTCATGCCTACTATAGCTGGCACCGGAATGATGATGGAGCTGCAGTCAAAGCAGATTCTATGTGGTTTTTCAACAGATATCATCATCCTGTTGATGACTCCACAAGTTCCCAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0005488 GO:0005506 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008199 GO:0008270 GO:0009267 GO:0009505 GO:0009506 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0030054 GO:0030145 GO:0030312 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042578 GO:0042594 GO:0043167 GO:0043169 GO:0044237 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0055044 GO:0071496 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

463

Amino Acids

52.71

Weight (kDa)

6.46

Isoelectric Point (pI)

40.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	54 - 144	5.9e-21	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	155 - 353	1.3e-25	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	379 - 437	5.5e-21	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16430 AT2G16430
fragaria_vesca	FvH4_2g09890 FvH4_2g09920 FvH4_2g09920 FvH4_2g09920
malus_domestica	MD05G1058900.v1.1 MD05G1059100.v1.1 MD05G1059300.v1.1 MD06G1221800.v1.1 MD10G1067300.v1.1
prunus_persica	Prupe.8G088800_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G088900_v2.0.a1 Prupe.8G089000_v2.0.a1 Prupe.8G089100_v2.0.a1 Prupe.8G089200_v2.0.a1
pyrus_communis	pycom05g05060 pycom05g05070 pycom10g05330
rosa_chinensis	RchiOBHm_Chr5g0073481 RchiOBHm_Chr6g0266421 RchiOBHm_Chr6g0266561 RchiOBHm_Chr6g0266681
rosa_laevigata	RLG00000014085 RLG00000014091 RLG00000014096
rosa_multiflora	Rmu_sc0003605.1_g000010 Rmu_sc0006413.1_g000013 Rmu_sc0022454.1_g000004 Rmu_sc0023098.1_g000001 Rmu_ssc0000226.1_g000024
rosa_roxburghii	Rroxscaffold_1G00007780 Rroxscaffold_7G00201560 Rroxscaffold_7G00201660
rosa_rugosa	Rorug05G0423800 Rorug06G0025500 Rorug06G0026200
rosa_samantha	Rh5AG481100 Rh5BG501800 Rh5CG525000 Rh5DG514200 Rh6AG146200 Rh6AG146700 Rh6AG147400 Rh6BG146400 Rh6BG147800 Rh6CG142800 Rh6CG143600 Rh6DG131000 Rh6DG132400
rosa_wichuraiana	Rw5G044690 Rw6G012590 Rw6G012600 Rw6G012660 Rw6G012700 Rw6G012810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1290
AccB7I	CCANNNNNTGG	1 cut(s)	707
AccI	GTMKAC	2 cut(s)	746, 1143
AccIII	TCCGGA	1 cut(s)	226
AciI	CCGC	1 cut(s)	14
AclWI	GGATC	1 cut(s)	227
AcoI	YGGCCR	1 cut(s)	400
AcsI	RAATTY	1 cut(s)	138
AcuI	CTGAAG	1 cut(s)	792
AfaI	GTAC	4 cut(s)	103, 775, 791, 947
AfiI	CCNNNNNNNGG	3 cut(s)	443, 542, 707
AflIII	ACRYGT	1 cut(s)	1045
AgeI	ACCGGT	1 cut(s)	741
AgsI	TTSAA	5 cut(s)	504, 541, 727, 1175, 1342
AjnI	CCWGG	3 cut(s)	145, 235, 1004
AleI	CACNNNNGTG	1 cut(s)	83
AluBI	AGCT	8 cut(s)	45, 407, 806, 886, 954, 1220, 1287, 1310
AluI	AGCT	8 cut(s)	45, 407, 806, 886, 954, 1220, 1287, 1310
Alw21I	GWGCWC	1 cut(s)	369
Alw26I	GTCTC	2 cut(s)	902, 1191
AlwI	GGATC	1 cut(s)	227
Aor13HI	TCCGGA	1 cut(s)	226
AoxI	GGCC	5 cut(s)	148, 400, 446, 544, 1230
ApeKI	GCWGC	2 cut(s)	647, 1310
ApoI	RAATTY	1 cut(s)	138
AsiGI	ACCGGT	1 cut(s)	741
AspS9I	GGNCC	1 cut(s)	446
AsuC2I	CCSGG	2 cut(s)	902, 1118
AsuHPI	GGTGA	4 cut(s)	332, 578, 722, 1132
BalI	TGGCCA	1 cut(s)	402
BanI	GGYRCC	1 cut(s)	1290
BbsI	GAAGAC	2 cut(s)	464, 822
Bbv12I	GWGCWC	1 cut(s)	369
BbvI	GCAGC	2 cut(s)	634, 1297
BccI	CCATC	5 cut(s)	4, 359, 597, 1154, 1298
BceAI	ACGGC	1 cut(s)	216
BciT130I	CCWGG	3 cut(s)	147, 237, 1006
BciVI	GTATCC	1 cut(s)	610
BcnI	CCSGG	2 cut(s)	902, 1118
BcoDI	GTCTC	2 cut(s)	902, 1191
BfaI	CTAG	1 cut(s)	243
BfmI	CTRYAG	4 cut(s)	438, 672, 1282, 1311
BfuI	GTATCC	1 cut(s)	610
BisI	GCNGC	2 cut(s)	648, 1311
BlpI	GCTNAGC	1 cut(s)	1180
BlsI	GCNGC	2 cut(s)	649, 1312
BmcAI	AGTACT	2 cut(s)	103, 775
Bme1390I	CCNGG	5 cut(s)	147, 237, 902, 1006, 1118
BmgT120I	GGNCC	1 cut(s)	446
BmiI	GGNNCC	1 cut(s)	1292
BmrFI	CCNGG	5 cut(s)	147, 237, 902, 1006, 1118
BmsI	GCATC	3 cut(s)	118, 442, 773
BpiI	GAAGAC	2 cut(s)	464, 822
BplI	GAGNNNNNCTC	2 cut(s)	763, 795
BpmI	CTGGAG	2 cut(s)	129, 896
Bpu1102I	GCTNAGC	1 cut(s)	1180
BpuMI	CCSGG	2 cut(s)	902, 1118
BsaBI	GATNNNNATC	1 cut(s)	588
BsaI	GGTCTC	1 cut(s)	1191
BsaJI	CCNNGG	4 cut(s)	659, 914, 941, 1116
BsaWI	WCCGGW	3 cut(s)	226, 741, 1293
BsaXI	ACNNNNNCTCC	2 cut(s)	1299, 1329
Bsc4I	CCNNNNNNNGG	3 cut(s)	443, 542, 707
Bse118I	RCCGGY	1 cut(s)	741
Bse1I	ACTGG	1 cut(s)	1233
Bse3DI	GCAATG	2 cut(s)	57, 210
Bse8I	GATNNNNATC	1 cut(s)	588
BseAI	TCCGGA	1 cut(s)	226
BseBI	CCWGG	3 cut(s)	147, 237, 1006
BseDI	CCNNGG	4 cut(s)	659, 914, 941, 1116
BseGI	GGATG	4 cut(s)	235, 589, 910, 1354
BseJI	GATNNNNATC	1 cut(s)	588
BseLI	CCNNNNNNNGG	3 cut(s)	443, 542, 707
BseMI	GCAATG	2 cut(s)	57, 210
BseMII	CTCAG	6 cut(s)	87, 399, 807, 875, 1059, 1230
BseNI	ACTGG	1 cut(s)	1233
BseRI	GAGGAG	2 cut(s)	423, 849
BseXI	GCAGC	2 cut(s)	634, 1297
BsgI	GTGCAG	2 cut(s)	331, 1119
BshFI	GGCC	5 cut(s)	150, 402, 448, 546, 1232
BshNI	GGYRCC	1 cut(s)	1290
BshTI	ACCGGT	1 cut(s)	741
BsiHKAI	GWGCWC	1 cut(s)	369
BsiSI	CCGG	5 cut(s)	227, 742, 901, 1118, 1294
BslFI	GGGAC	1 cut(s)	211
BslI	CCNNNNNNNGG	3 cut(s)	443, 542, 707
BsmAI	GTCTC	2 cut(s)	902, 1191
BsmFI	GGGAC	1 cut(s)	211
BsmI	GAATGC	1 cut(s)	934
BsnI	GGCC	5 cut(s)	150, 402, 448, 546, 1232
Bso31I	GGTCTC	1 cut(s)	1191
Bsp1286I	GDGCHC	1 cut(s)	369
Bsp13I	TCCGGA	1 cut(s)	226
Bsp143I	GATC	4 cut(s)	187, 219, 1064, 1126
Bsp1720I	GCTNAGC	1 cut(s)	1180
Bsp19I	CCATGG	2 cut(s)	914, 941
BspACI	CCGC	1 cut(s)	14
BspANI	GGCC	5 cut(s)	150, 402, 448, 546, 1232
BspCNI	CTCAG	6 cut(s)	88, 400, 806, 874, 1060, 1229
BspEI	TCCGGA	1 cut(s)	226
BspHI	TCATGA	2 cut(s)	598, 682
BspLI	GGNNCC	1 cut(s)	1292
BspMAI	CTGCAG	3 cut(s)	442, 676, 1315
BspPI	GGATC	1 cut(s)	227
BspT107I	GGYRCC	1 cut(s)	1290
BspTNI	GGTCTC	1 cut(s)	1191
BsrDI	GCAATG	2 cut(s)	57, 210
BsrFI	RCCGGY	1 cut(s)	741
BsrI	ACTGG	1 cut(s)	1233
BssAI	RCCGGY	1 cut(s)	741
BssECI	CCNNGG	4 cut(s)	659, 914, 941, 1116
BssMI	GATC	4 cut(s)	187, 219, 1064, 1126
BssNAI	GTATAC	1 cut(s)	747
BssT1I	CCWWGG	3 cut(s)	659, 914, 941
Bst1107I	GTATAC	1 cut(s)	747
Bst2UI	CCWGG	3 cut(s)	147, 237, 1006
Bst4CI	ACNGT	3 cut(s)	106, 553, 848
BstC8I	GCNNGC	1 cut(s)	1289
BstDEI	CTNAG	7 cut(s)	96, 408, 793, 861, 1068, 1180, 1216
BstDSI	CCRYGG	2 cut(s)	914, 941
BstEII	GGTNACC	1 cut(s)	566
BstF5I	GGATG	4 cut(s)	235, 589, 910, 1354
BstKTI	GATC	4 cut(s)	190, 222, 1067, 1129
BstMAI	GTCTC	2 cut(s)	902, 1191
BstMBI	GATC	4 cut(s)	187, 219, 1064, 1126
BstMWI	GCNNNNNNNGC	2 cut(s)	736, 1319
BstNI	CCWGG	3 cut(s)	147, 237, 1006
BstNSI	RCATGY	1 cut(s)	1049
BstPI	GGTNACC	1 cut(s)	566
BstSCI	CCNGG	5 cut(s)	145, 235, 900, 1004, 1116
BstSFI	CTRYAG	4 cut(s)	438, 672, 1282, 1311
BstV1I	GCAGC	2 cut(s)	634, 1297
BstV2I	GAAGAC	2 cut(s)	464, 822
BstZ17I	GTATAC	1 cut(s)	747
BsuI	GTATCC	1 cut(s)	610
BsuRI	GGCC	5 cut(s)	150, 402, 448, 546, 1232
BtgI	CCRYGG	2 cut(s)	914, 941
BtgZI	GCGATG	1 cut(s)	1173
BtsCI	GGATG	4 cut(s)	235, 589, 910, 1354
BtsI	GCAGTG	1 cut(s)	343
BtsIMutI	CAGTG	1 cut(s)	343
Cac8I	GCNNGC	1 cut(s)	1289
CciI	TCATGA	2 cut(s)	598, 682
Cfr10I	RCCGGY	1 cut(s)	741
Cfr13I	GGNCC	1 cut(s)	446
CsiI	ACCWGGT	1 cut(s)	235
Csp6I	GTAC	4 cut(s)	102, 774, 790, 946
CspAI	ACCGGT	1 cut(s)	741
CspCI	CAANNNNNGTGG	2 cut(s)	593, 628
CviQI	GTAC	4 cut(s)	102, 774, 790, 946
DdeI	CTNAG	7 cut(s)	96, 408, 793, 861, 1068, 1180, 1216
DpnI	GATC	4 cut(s)	189, 221, 1066, 1128
DpnII	GATC	4 cut(s)	187, 219, 1064, 1126
DraI	TTTAAA	1 cut(s)	760
EaeI	YGGCCR	1 cut(s)	400
Eco130I	CCWWGG	3 cut(s)	659, 914, 941
Eco31I	GGTCTC	1 cut(s)	1191
Eco32I	GATATC	1 cut(s)	1349
Eco57I	CTGAAG	1 cut(s)	792
Eco91I	GGTNACC	1 cut(s)	566
EcoO65I	GGTNACC	1 cut(s)	566
EcoRII	CCWGG	3 cut(s)	145, 235, 1004
EcoRV	GATATC	1 cut(s)	1349
EcoT14I	CCWWGG	3 cut(s)	659, 914, 941
ErhI	CCWWGG	3 cut(s)	659, 914, 941
FalI	AAGNNNNNCTT	2 cut(s)	634, 666
FaqI	GGGAC	1 cut(s)	211
FblI	GTMKAC	2 cut(s)	746, 1143
Fnu4HI	GCNGC	2 cut(s)	648, 1311
FokI	GGATG	4 cut(s)	242, 576, 917, 1341
Fsp4HI	GCNGC	2 cut(s)	648, 1311
FspBI	CTAG	1 cut(s)	243
GluI	GCNGC	2 cut(s)	648, 1311
GsuI	CTGGAG	2 cut(s)	129, 896
HaeIII	GGCC	5 cut(s)	150, 402, 448, 546, 1232
HapII	CCGG	5 cut(s)	227, 742, 901, 1118, 1294
HincII	GTYRAC	1 cut(s)	898
HindII	GTYRAC	1 cut(s)	898
HinfI	GANTC	7 cut(s)	500, 527, 664, 990, 1001, 1325, 1367
HpaI	GTTAAC	1 cut(s)	898
HpaII	CCGG	5 cut(s)	227, 742, 901, 1118, 1294
HphI	GGTGA	4 cut(s)	332, 578, 722, 1132
Hpy166II	GTNNAC	3 cut(s)	747, 898, 1144
Hpy188I	TCNGA	2 cut(s)	356, 1069
Hpy188III	TCNNGA	7 cut(s)	191, 227, 599, 683, 799, 1261, 1386
Hpy8I	GTNNAC	3 cut(s)	747, 898, 1144
HpyAV	CCTTC	6 cut(s)	142, 188, 277, 621, 968, 1169
HpyCH4III	ACNGT	3 cut(s)	106, 553, 848
HpyCH4IV	ACGT	1 cut(s)	620
HpyCH4V	TGCA	9 cut(s)	158, 348, 440, 674, 698, 934, 1088, 1136, 1313
HpyF10VI	GCNNNNNNNGC	2 cut(s)	736, 1319
HpyF3I	CTNAG	7 cut(s)	96, 408, 793, 861, 1068, 1180, 1216
HpySE526I	ACGT	1 cut(s)	620
Kpn2I	TCCGGA	1 cut(s)	226
KspAI	GTTAAC	1 cut(s)	898
Kzo9I	GATC	4 cut(s)	187, 219, 1064, 1126
LmnI	GCTCC	5 cut(s)	261, 784, 877, 883, 1307
Lsp1109I	GCAGC	2 cut(s)	634, 1297
LweI	GCATC	3 cut(s)	118, 442, 773
MabI	ACCWGGT	1 cut(s)	235
MaeI	CTAG	1 cut(s)	243
MaeII	ACGT	1 cut(s)	620
MaeIII	GTNAC	3 cut(s)	332, 427, 566
MalI	GATC	4 cut(s)	189, 221, 1066, 1128
MboI	GATC	4 cut(s)	187, 219, 1064, 1126
MboII	GAAGA	3 cut(s)	19, 464, 822
MfeI	CAATTG	1 cut(s)	1152
MhlI	GDGCHC	1 cut(s)	369
MlsI	TGGCCA	1 cut(s)	402
MluCI	AATT	6 cut(s)	138, 359, 396, 705, 995, 1152
MluNI	TGGCCA	1 cut(s)	402
MlyI	GAGTC	3 cut(s)	999, 1010, 1361
Mox20I	TGGCCA	1 cut(s)	402
MroI	TCCGGA	1 cut(s)	226
MscI	TGGCCA	1 cut(s)	402
MseI	TTAA	3 cut(s)	609, 759, 897
MslI	CAYNNNNRTG	3 cut(s)	83, 1050, 1296
Msp20I	TGGCCA	1 cut(s)	402
MspI	CCGG	5 cut(s)	227, 742, 901, 1118, 1294
MspR9I	CCNGG	5 cut(s)	147, 237, 902, 1006, 1118
MunI	CAATTG	1 cut(s)	1152
Mva1269I	GAATGC	1 cut(s)	934
MvaI	CCWGG	3 cut(s)	147, 237, 1006
MwoI	GCNNNNNNNGC	2 cut(s)	736, 1319
NciI	CCSGG	2 cut(s)	902, 1118
NcoI	CCATGG	2 cut(s)	914, 941
NdeII	GATC	4 cut(s)	187, 219, 1064, 1126
NlaIV	GGNNCC	1 cut(s)	1292
NmuCI	GTSAC	1 cut(s)	566
NspI	RCATGY	1 cut(s)	1049
OliI	CACNNNNGTG	1 cut(s)	83
PagI	TCATGA	2 cut(s)	598, 682
PciI	ACATGT	1 cut(s)	1045
PcsI	WCGNNNNNNNCGW	1 cut(s)	1069
PctI	GAATGC	1 cut(s)	934
PfeI	GAWTC	4 cut(s)	500, 527, 664, 1325
PflMI	CCANNNNNTGG	1 cut(s)	707
PinAI	ACCGGT	1 cut(s)	741
PkrI	GCNGC	2 cut(s)	649, 1312
PleI	GAGTC	3 cut(s)	998, 1009, 1361
PpsI	GAGTC	3 cut(s)	998, 1009, 1361
PscI	ACATGT	1 cut(s)	1045
Psp6I	CCWGG	3 cut(s)	145, 235, 1004
PspEI	GGTNACC	1 cut(s)	566
PspGI	CCWGG	3 cut(s)	145, 235, 1004
PspN4I	GGNNCC	1 cut(s)	1292
PspPI	GGNCC	1 cut(s)	446
PstI	CTGCAG	3 cut(s)	442, 676, 1315
RsaI	GTAC	4 cut(s)	103, 775, 791, 947
RsaNI	GTAC	4 cut(s)	102, 774, 790, 946
RseI	CAYNNNNRTG	3 cut(s)	83, 1050, 1296
SaqAI	TTAA	3 cut(s)	609, 759, 897
SatI	GCNGC	2 cut(s)	648, 1311
Sau3AI	GATC	4 cut(s)	187, 219, 1064, 1126
Sau96I	GGNCC	1 cut(s)	446
ScaI	AGTACT	2 cut(s)	103, 775
SchI	GAGTC	3 cut(s)	999, 1010, 1361
ScrFI	CCNGG	5 cut(s)	147, 237, 902, 1006, 1118
SduI	GDGCHC	1 cut(s)	369
SexAI	ACCWGGT	1 cut(s)	235
SfaNI	GCATC	3 cut(s)	118, 442, 773
SfcI	CTRYAG	4 cut(s)	438, 672, 1282, 1311
SmiMI	CAYNNNNRTG	3 cut(s)	83, 1050, 1296
Sse9I	AATT	6 cut(s)	138, 359, 396, 705, 995, 1152
SsiI	CCGC	1 cut(s)	14
SspI	AATATT	2 cut(s)	380, 1084
SspMI	CTAG	1 cut(s)	243
StyD4I	CCNGG	5 cut(s)	145, 235, 900, 1004, 1116
StyI	CCWWGG	3 cut(s)	659, 914, 941
TaaI	ACNGT	3 cut(s)	106, 553, 848
TaiI	ACGT	1 cut(s)	623
TaqI	TCGA	4 cut(s)	108, 999, 1026, 1098
TasI	AATT	6 cut(s)	138, 359, 396, 705, 995, 1152
TatI	WGTACW	2 cut(s)	101, 773
TfiI	GAWTC	4 cut(s)	500, 527, 664, 1325
Tru1I	TTAA	3 cut(s)	609, 759, 897
Tru9I	TTAA	3 cut(s)	609, 759, 897
TscAI	CASTG	1 cut(s)	350
TseFI	GTSAC	1 cut(s)	566
TseI	GCWGC	2 cut(s)	647, 1310
Tsp45I	GTSAC	1 cut(s)	566
TspDTI	ATGAA	6 cut(s)	135, 161, 246, 622, 656, 822
TspGWI	ACGGA	1 cut(s)	744
TspRI	CASTG	1 cut(s)	350
Van91I	CCANNNNNTGG	1 cut(s)	707
XapI	RAATTY	1 cut(s)	138
XceI	RCATGY	1 cut(s)	1049
XmiI	GTMKAC	2 cut(s)	746, 1143
XspI	CTAG	1 cut(s)	243
ZrmI	AGTACT	2 cut(s)	103, 775

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.