FvH4_2g15660

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003022
Physical Location & Seq
Reverse (-)
13738657 .. 13742245
3589 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15660.t4

Sequence Viewer

Length: 477 bp
ATGGCAGCTGTTTCGCAGTCTGTGGTAACCTGTTCAGGTTTGATTCATCATCCAGGTCATGCTAAACTACCGTTTGGACATAGGGGGACCAAGATCACTGATCACAACGCCATTTCAGTGACGTGTAGAAGGATTCGTAGAGAAGGGATTCAATGTTTGGCTGTACGTCGGGTTGGCACCGCATATGCTGCTTCTGTGGGGAAGGATCATCATCAGTTGAGTGCTGATGATGGAGTACCTCAGGAGCCCTTCCTTCTGAGTCTTTTCAAAGAAACTGTGTGGTCTTTGAGGTCATTATTCAATTTTCTGCTTGAGCAGCCAAGCCAGCTGAAGTACATTGAATGGCCAGGTTTCCGTAGCACGCTGAAGACTGCGACTCTCACTCTTGTTCTTGTGGCGTTGCTCATCGTAGCACTTTCATCAACTGACTCTGCCCTCAGTTATTTGTTGGCTTTGATTCTCAGGAGAAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

159

Amino Acids

17.41

Weight (kDa)

9.87

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 101 - 154 5.5e-07 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AciI CCGC 1 cut(s) 180
AclWI GGATC 1 cut(s) 213
AcoI YGGCCR 1 cut(s) 344
AcuI CTGAAG 2 cut(s) 350, 386
AfaI GTAC 3 cut(s) 165, 237, 335
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 4 cut(s) 152, 268, 301, 341
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 2 cut(s) 52, 346
AluBI AGCT 2 cut(s) 8, 328
AluI AGCT 2 cut(s) 8, 328
AlwI GGATC 1 cut(s) 213
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 3 cut(s) 5, 188, 316
Asp700I GAANNNNTTC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 87
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 240
BalI TGGCCA 1 cut(s) 346
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 17, 175, 328
BccI CCATC 1 cut(s) 224
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 2 cut(s) 54, 348
BclI TGATCA 1 cut(s) 100
BisI GCNGC 3 cut(s) 6, 189, 317
BlsI GCNGC 3 cut(s) 7, 190, 318
Bme1390I CCNGG 2 cut(s) 54, 348
Bme18I GGWCC 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 88, 178, 246
BmrFI CCNGG 2 cut(s) 54, 348
BpiI GAAGAC 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 332
BsaBI GATNNNNATC 1 cut(s) 210
Bse21I CCTNAGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 2 cut(s) 54, 348
BseGI GGATG 1 cut(s) 49
BseJI GATNNNNATC 1 cut(s) 210
BseMII CTCAG 4 cut(s) 248, 254, 451, 475
BseXI GCAGC 3 cut(s) 17, 175, 328
BshFI GGCC 1 cut(s) 346
BshNI GGYRCC 1 cut(s) 176
BslFI GGGAC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 3 cut(s) 93, 100, 205
BspACI CCGC 1 cut(s) 180
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 4 cut(s) 249, 253, 450, 474
BspLI GGNNCC 3 cut(s) 88, 178, 246
BspPI GGATC 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 176
BssMI GATC 3 cut(s) 93, 100, 205
Bst2UI CCWGG 2 cut(s) 54, 348
Bst4CI ACNGT 2 cut(s) 72, 277
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 326, 362
BstDEI CTNAG 4 cut(s) 240, 257, 437, 461
BstEII GGTNACC 1 cut(s) 25
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 3 cut(s) 96, 103, 208
BstMBI GATC 3 cut(s) 93, 100, 205
BstMWI GCNNNNNNNGC 3 cut(s) 188, 316, 325
BstNI CCWGG 2 cut(s) 54, 348
BstPI GGTNACC 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 52, 346
BstV1I GCAGC 3 cut(s) 17, 175, 328
BstV2I GAAGAC 1 cut(s) 374
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 1 cut(s) 346
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 96, 123
Cac8I GCNNGC 2 cut(s) 326, 362
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 3 cut(s) 164, 236, 334
CviAII CATG 1 cut(s) 59
CviJI RGCY 8 cut(s) 8, 161, 247, 319, 324, 328, 346, 452
CviKI_1 RGCY 8 cut(s) 8, 161, 247, 319, 324, 328, 346, 452
CviQI GTAC 3 cut(s) 164, 236, 334
DdeI CTNAG 4 cut(s) 240, 257, 437, 461
DpnI GATC 3 cut(s) 95, 102, 207
DpnII GATC 3 cut(s) 93, 100, 205
EaeI YGGCCR 1 cut(s) 344
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 350, 386
Eco81I CCTNAGG 1 cut(s) 240
Eco91I GGTNACC 1 cut(s) 25
EcoO65I GGTNACC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 52, 346
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 1 cut(s) 62
FaiI YATR 5 cut(s) 60, 81, 184, 186, 475
FaqI GGGAC 1 cut(s) 100
FatI CATG 1 cut(s) 58
FauNDI CATATG 1 cut(s) 184
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 6, 189, 317
FokI GGATG 1 cut(s) 36
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 6, 189, 317
GluI GCNGC 3 cut(s) 6, 189, 317
HaeIII GGCC 1 cut(s) 346
Hin1II CATG 1 cut(s) 62
HinfI GANTC 7 cut(s) 43, 133, 148, 259, 376, 428, 457
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 242, 463
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 5 cut(s) 123, 137, 196, 259, 263
HpyCH4III ACNGT 2 cut(s) 72, 277
HpyCH4IV ACGT 2 cut(s) 122, 166
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 316, 325
HpyF3I CTNAG 4 cut(s) 240, 257, 437, 461
HpySE526I ACGT 2 cut(s) 122, 166
Hsp92II CATG 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 3 cut(s) 93, 100, 205
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 9 cut(s) 21, 39, 43, 66, 227, 333, 338, 360, 448
Lsp1109I GCAGC 3 cut(s) 17, 175, 328
MaeII ACGT 2 cut(s) 122, 166
MaeIII GTNAC 2 cut(s) 25, 118
MalI GATC 3 cut(s) 95, 102, 207
MboI GATC 3 cut(s) 93, 100, 205
MboII GAAGA 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 1 cut(s) 301
MluNI TGGCCA 1 cut(s) 346
MlyI GAGTC 3 cut(s) 268, 370, 422
MnlI CCTC 3 cut(s) 249, 282, 446
Mox20I TGGCCA 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 1 cut(s) 346
MslI CAYNNNNRTG 1 cut(s) 116
Msp20I TGGCCA 1 cut(s) 346
MspA1I CMGCKG 2 cut(s) 8, 328
MspR9I CCNGG 2 cut(s) 54, 348
MvaI CCWGG 2 cut(s) 54, 348
MwoI GCNNNNNNNGC 3 cut(s) 188, 316, 325
NdeI CATATG 1 cut(s) 184
NdeII GATC 3 cut(s) 93, 100, 205
NlaIII CATG 1 cut(s) 62
NlaIV GGNNCC 3 cut(s) 88, 178, 246
NmuCI GTSAC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 4 cut(s) 43, 133, 148, 457
PkrI GCNGC 3 cut(s) 7, 190, 318
PleI GAGTC 3 cut(s) 267, 370, 422
PpsI GAGTC 3 cut(s) 267, 370, 422
Psp6I CCWGG 2 cut(s) 52, 346
PspEI GGTNACC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 52, 346
PspN4I GGNNCC 3 cut(s) 88, 178, 246
PspPI GGNCC 1 cut(s) 87
PvuII CAGCTG 2 cut(s) 8, 328
RsaI GTAC 3 cut(s) 165, 237, 335
RsaNI GTAC 3 cut(s) 164, 236, 334
RseI CAYNNNNRTG 1 cut(s) 116
SatI GCNGC 3 cut(s) 6, 189, 317
Sau3AI GATC 3 cut(s) 93, 100, 205
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 3 cut(s) 268, 370, 422
ScrFI CCNGG 2 cut(s) 54, 348
SduI GDGCHC 1 cut(s) 249
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 1 cut(s) 301
SsiI CCGC 1 cut(s) 180
StyD4I CCNGG 2 cut(s) 52, 346
TaaI ACNGT 2 cut(s) 72, 277
TaiI ACGT 2 cut(s) 125, 169
TasI AATT 1 cut(s) 301
TatI WGTACW 1 cut(s) 333
TfiI GAWTC 4 cut(s) 43, 133, 148, 457
TscAI CASTG 2 cut(s) 103, 123
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 3 cut(s) 5, 188, 316
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 35, 408
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 2 cut(s) 103, 123
VpaK11BI GGWCC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.