Rroxscaffold_7G00189390

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003022
Physical Location & Seq
Forward (+)
29136751 .. 29138300
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189390.1

Sequence Viewer

Length: 459 bp
ATGGCGGCTGTTTCACAGTCCTTGGCAACCTGTTCAGGTTATGCTAAACTGCCATTTGGACATAACAGGACCAAGATCACTAATCACAGTGCCATTTCAGTAACATATAGAAGGATTTGTAGGGCAGGAATTAGATGTTTGGCTGTATGTCGGGTTGGCACCACATGTGCTGCGTCTGTGGGAAAGGATTATCATCAGTTGAGCGTTGATGATGGAGTACCTCAGGAGCCCTTCCTTCTGAGTCTTTTCAAAGAAACAGTGTGGTCTTTGAGATCATTATTCATTTTCCTGCTTGAGCAGCCGAGTCAGCTGAAGTACATTGAATGGCCAGGTTTCCGTAGCACGCTGAAGACTGCGACTTTGACTCTTGTTCTTGTGGTGTTGCTCATTGTTGCACTTTCATCAACTGACTCTGCCCTCAGCTATTTGTTGGCTTTGATTATCAGGAGAAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

152

Amino Acids

16.78

Weight (kDa)

9.67

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 96 - 148 1.2e-06 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 326
AcuI CTGAAG 2 cut(s) 332, 368
AfaI GTAC 2 cut(s) 219, 317
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 2 cut(s) 250, 323
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 2 cut(s) 310, 423
AluI AGCT 2 cut(s) 310, 423
AoxI GGCC 1 cut(s) 326
ApeKI GCWGC 2 cut(s) 170, 298
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AspS9I GGNCC 1 cut(s) 69
AvaII GGWCC 1 cut(s) 69
AxyI CCTNAGG 1 cut(s) 222
BalI TGGCCA 1 cut(s) 328
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 356
BbvCI CCTCAGC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 157, 310
BccI CCATC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 330
BisI GCNGC 3 cut(s) 6, 171, 299
BlsI GCNGC 3 cut(s) 7, 172, 300
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 2 cut(s) 160, 228
BmrFI CCNGG 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 356
Bpu10I CCTNAGC 1 cut(s) 419
BpuEI CTTGAG 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 21
Bse21I CCTNAGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 21
BseJI GATNNNNATC 1 cut(s) 192
BseMII CTCAG 3 cut(s) 230, 236, 433
BseXI GCAGC 2 cut(s) 157, 310
BshFI GGCC 1 cut(s) 328
BshNI GGYRCC 1 cut(s) 158
BsnI GGCC 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 2 cut(s) 75, 272
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 328
BspCNI CTCAG 3 cut(s) 231, 235, 432
BspLI GGNNCC 2 cut(s) 160, 228
BspT107I GGYRCC 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 75, 272
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 3 cut(s) 18, 89, 259
BstC8I GCNNGC 1 cut(s) 344
BstDEI CTNAG 3 cut(s) 222, 239, 419
BstKTI GATC 2 cut(s) 78, 275
BstMBI GATC 2 cut(s) 75, 272
BstMWI GCNNNNNNNGC 2 cut(s) 298, 307
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 328
BstV1I GCAGC 2 cut(s) 157, 310
BstV2I GAAGAC 1 cut(s) 356
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 1 cut(s) 328
BtsIMutI CAGTG 2 cut(s) 94, 264
Cac8I GCNNGC 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 69
CseI GACGC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 218, 316
CviAII CATG 1 cut(s) 165
CviJI RGCY 8 cut(s) 8, 143, 229, 301, 310, 328, 423, 434
CviKI_1 RGCY 8 cut(s) 8, 143, 229, 301, 310, 328, 423, 434
CviQI GTAC 2 cut(s) 218, 316
DdeI CTNAG 3 cut(s) 222, 239, 419
DpnI GATC 2 cut(s) 77, 274
DpnII GATC 2 cut(s) 75, 272
EaeI YGGCCR 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 69
Eco57I CTGAAG 2 cut(s) 332, 368
Eco81I CCTNAGG 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 168
FaiI YATR 7 cut(s) 42, 63, 106, 108, 148, 166, 457
FatI CATG 1 cut(s) 164
Fnu4HI GCNGC 3 cut(s) 6, 171, 299
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 3 cut(s) 6, 171, 299
GluI GCNGC 3 cut(s) 6, 171, 299
HaeIII GGCC 1 cut(s) 328
HgaI GACGC 1 cut(s) 162
Hin1II CATG 1 cut(s) 168
HinfI GANTC 4 cut(s) 241, 304, 364, 410
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 2 cut(s) 224, 445
HpyAV CCTTC 3 cut(s) 105, 241, 245
HpyCH4III ACNGT 3 cut(s) 18, 89, 259
HpyCH4V TGCA 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 307
HpyF3I CTNAG 3 cut(s) 222, 239, 419
Hsp92II CATG 1 cut(s) 168
Kzo9I GATC 2 cut(s) 75, 272
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 9 cut(s) 21, 43, 52, 111, 209, 302, 315, 342, 430
Lsp1109I GCAGC 2 cut(s) 157, 310
MaeIII GTNAC 1 cut(s) 100
MalI GATC 2 cut(s) 77, 274
MboI GATC 2 cut(s) 75, 272
MboII GAAGA 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 328
MluCI AATT 1 cut(s) 129
MluNI TGGCCA 1 cut(s) 328
MlyI GAGTC 4 cut(s) 250, 313, 358, 404
MnlI CCTC 2 cut(s) 231, 428
Mox20I TGGCCA 1 cut(s) 328
MscI TGGCCA 1 cut(s) 328
Msp20I TGGCCA 1 cut(s) 328
MspA1I CMGCKG 1 cut(s) 310
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 2 cut(s) 298, 307
NdeII GATC 2 cut(s) 75, 272
NlaIII CATG 1 cut(s) 168
NlaIV GGNNCC 2 cut(s) 160, 228
NmeAIII GCCGAG 1 cut(s) 327
NspI RCATGY 1 cut(s) 168
PciI ACATGT 1 cut(s) 164
PkrI GCNGC 3 cut(s) 7, 172, 300
PleI GAGTC 4 cut(s) 249, 312, 358, 404
PpsI GAGTC 4 cut(s) 249, 312, 358, 404
PscI ACATGT 1 cut(s) 164
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 160, 228
PspPI GGNCC 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 2 cut(s) 219, 317
RsaNI GTAC 2 cut(s) 218, 316
SatI GCNGC 3 cut(s) 6, 171, 299
Sau3AI GATC 2 cut(s) 75, 272
Sau96I GGNCC 1 cut(s) 69
SchI GAGTC 4 cut(s) 250, 313, 358, 404
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 1 cut(s) 231
SetI ASST 6 cut(s) 32, 40, 223, 312, 334, 425
SinI GGWCC 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 1 cut(s) 129
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 1 cut(s) 328
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 3 cut(s) 18, 89, 259
TasI AATT 1 cut(s) 129
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 8
TscAI CASTG 2 cut(s) 94, 264
TseI GCWGC 2 cut(s) 170, 298
TspDTI ATGAA 2 cut(s) 271, 390
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 94, 264
VpaK11BI GGWCC 1 cut(s) 69
XceI RCATGY 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.