Rw6G020170

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003022
Physical Location & Seq
Reverse (-)
40369666 .. 40370576
911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G020170.1

Sequence Viewer

Length: 468 bp
ATGGCGGCTGTTTCGCAGTCCGTGGCAACCTGTTCAGGTTTGATTAATCATCCAGGGCATGCTAAACTACCATTTGGACATAACGGGACCAAGATCACTAATCACAATGCCGTTTCAGTAACATGTAGAAGGACTCGTAGGGCAGGGATTAGATGTTTGGCTGTACGTCGGGTTGGCACCACATATGCTGCATCTGTGGGAAAGGATCATCATCAATTGAGTGTTGATGATGGAGTACCTCAGGAGCCCTTCCTTCTAAGTCTTTTCAAAGAAACACTAATTGGTGTGTTTCTACTTTTTCTTGAGCAGCCGAGTCAGCTGAAGTACATTGAATGGCCAGGTTTCCGTAGCACGCTGAAGACTGCGACTCTTACTCTTGTTCTTGTGGTGTTGCTCATTGTTGCACTTTCATCAACTGACTCTGCCCTCAGCTATTTGTTGGCTTTGATTCTCTGGAGAAGACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

155

Amino Acids

16.95

Weight (kDa)

9.95

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 105 - 151 3.2e-06 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 213
AcoI YGGCCR 1 cut(s) 335
AcuI CTGAAG 2 cut(s) 341, 377
AfaI GTAC 3 cut(s) 165, 237, 326
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 2 cut(s) 268, 332
AjnI CCWGG 2 cut(s) 52, 337
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 2 cut(s) 319, 432
AluI AGCT 2 cut(s) 319, 432
AlwI GGATC 1 cut(s) 213
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 188, 307
AseI ATTAAT 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 87
AvaII GGWCC 1 cut(s) 87
AxyI CCTNAGG 1 cut(s) 240
BalI TGGCCA 1 cut(s) 337
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 1 cut(s) 365
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 2 cut(s) 175, 319
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 2 cut(s) 54, 339
BisI GCNGC 3 cut(s) 6, 189, 308
BlsI GCNGC 3 cut(s) 7, 190, 309
Bme1390I CCNGG 2 cut(s) 54, 339
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 3 cut(s) 88, 178, 246
BmrFI CCNGG 2 cut(s) 54, 339
BmsI GCATC 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 323
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 2 cut(s) 21, 53
Bse21I CCTNAGG 1 cut(s) 240
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 2 cut(s) 54, 339
BseDI CCNNGG 2 cut(s) 21, 53
BseGI GGATG 1 cut(s) 49
BseJI GATNNNNATC 1 cut(s) 210
BseMII CTCAG 2 cut(s) 254, 442
BseXI GCAGC 2 cut(s) 175, 319
BshFI GGCC 1 cut(s) 337
BshNI GGYRCC 1 cut(s) 176
BslFI GGGAC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 93, 205
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 253, 441
BspLI GGNNCC 3 cut(s) 88, 178, 246
BspPI GGATC 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 176
BssECI CCNNGG 2 cut(s) 21, 53
BssMI GATC 2 cut(s) 93, 205
Bst2UI CCWGG 2 cut(s) 54, 339
BstC8I GCNNGC 2 cut(s) 60, 353
BstDEI CTNAG 3 cut(s) 240, 257, 428
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 2 cut(s) 96, 208
BstMBI GATC 2 cut(s) 93, 205
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNI CCWGG 2 cut(s) 54, 339
BstNSI RCATGY 2 cut(s) 62, 126
BstSCI CCNGG 2 cut(s) 52, 337
BstV1I GCAGC 2 cut(s) 175, 319
BstV2I GAAGAC 1 cut(s) 365
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 60, 353
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 3 cut(s) 164, 236, 325
CviAII CATG 2 cut(s) 59, 123
CviJI RGCY 8 cut(s) 8, 161, 247, 310, 319, 337, 432, 443
CviKI_1 RGCY 8 cut(s) 8, 161, 247, 310, 319, 337, 432, 443
CviQI GTAC 3 cut(s) 164, 236, 325
DdeI CTNAG 3 cut(s) 240, 257, 428
DpnI GATC 2 cut(s) 95, 207
DpnII GATC 2 cut(s) 93, 205
EaeI YGGCCR 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 341, 377
Eco81I CCTNAGG 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 52, 337
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 2 cut(s) 62, 126
FaiI YATR 6 cut(s) 60, 81, 124, 184, 186, 466
FaqI GGGAC 1 cut(s) 100
FatI CATG 2 cut(s) 58, 122
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 6, 189, 308
FokI GGATG 1 cut(s) 36
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 6, 189, 308
GluI GCNGC 3 cut(s) 6, 189, 308
HaeIII GGCC 1 cut(s) 337
Hin1II CATG 2 cut(s) 62, 126
HinfI GANTC 5 cut(s) 133, 313, 367, 419, 448
Hpy188III TCNNGA 3 cut(s) 242, 302, 454
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 3 cut(s) 123, 259, 263
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 2 cut(s) 191, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 240, 257, 428
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 2 cut(s) 62, 126
Kzo9I GATC 2 cut(s) 93, 205
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 9 cut(s) 21, 39, 43, 66, 129, 227, 324, 351, 439
Lsp1109I GCAGC 2 cut(s) 175, 319
LweI GCATC 1 cut(s) 200
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 118
MalI GATC 2 cut(s) 95, 207
MboI GATC 2 cut(s) 93, 205
MboII GAAGA 1 cut(s) 370
MfeI CAATTG 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 249
MlsI TGGCCA 1 cut(s) 337
MluCI AATT 2 cut(s) 215, 279
MluNI TGGCCA 1 cut(s) 337
MlyI GAGTC 4 cut(s) 127, 322, 361, 413
MnlI CCTC 2 cut(s) 249, 437
Mox20I TGGCCA 1 cut(s) 337
MscI TGGCCA 1 cut(s) 337
MseI TTAA 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 337
MspA1I CMGCKG 1 cut(s) 319
MspR9I CCNGG 2 cut(s) 54, 339
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 2 cut(s) 54, 339
MwoI GCNNNNNNNGC 1 cut(s) 316
NdeI CATATG 1 cut(s) 184
NdeII GATC 2 cut(s) 93, 205
NlaIII CATG 2 cut(s) 62, 126
NlaIV GGNNCC 3 cut(s) 88, 178, 246
NmeAIII GCCGAG 1 cut(s) 336
NspI RCATGY 2 cut(s) 62, 126
PaeI GCATGC 1 cut(s) 62
PciI ACATGT 1 cut(s) 122
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 3 cut(s) 7, 190, 309
PleI GAGTC 4 cut(s) 127, 321, 361, 413
PpsI GAGTC 4 cut(s) 127, 321, 361, 413
PscI ACATGT 1 cut(s) 122
PshBI ATTAAT 1 cut(s) 45
Psp6I CCWGG 2 cut(s) 52, 337
PspGI CCWGG 2 cut(s) 52, 337
PspN4I GGNNCC 3 cut(s) 88, 178, 246
PspPI GGNCC 1 cut(s) 87
PvuII CAGCTG 1 cut(s) 319
RsaI GTAC 3 cut(s) 165, 237, 326
RsaNI GTAC 3 cut(s) 164, 236, 325
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 3 cut(s) 6, 189, 308
Sau3AI GATC 2 cut(s) 93, 205
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 4 cut(s) 127, 322, 361, 413
ScrFI CCNGG 2 cut(s) 54, 339
SduI GDGCHC 1 cut(s) 249
SetI ASST 7 cut(s) 32, 40, 169, 241, 321, 343, 434
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SphI GCATGC 1 cut(s) 62
Sse9I AATT 2 cut(s) 215, 279
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 2 cut(s) 52, 337
TaiI ACGT 1 cut(s) 169
TasI AATT 2 cut(s) 215, 279
TatI WGTACW 1 cut(s) 324
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseI GCWGC 2 cut(s) 188, 307
TspDTI ATGAA 1 cut(s) 399
TspGWI ACGGA 2 cut(s) 10, 335
VpaK11BI GGWCC 1 cut(s) 87
VspI ATTAAT 1 cut(s) 45
XceI RCATGY 2 cut(s) 62, 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.