MD10G1127900.v1.1

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003022
Physical Location & Seq
Reverse (-)
20810148 .. 20812654
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1127900.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGGAAGAGGGATTCAATATTTGACTGCACATCGTGTTGGCACCATATGTGCTGCATCTGTGGGAAGGGATCATCGTCAATTGAGAATTGATGACGATTCACCTCAGGAGCCCTACTTTTTAAGTCTTGTCAAAGAAGCAGTGCGGGGGTTGAGATCATTATTCGTTTTTCTGGTTGAGCAGCCTAGTCAGCTGAAGTACATTGAATGGCCAGGCTTCCGTAGCACGCTTAAGACTGCTACATTTACACTTGTTCTTGTGGCACTACTCATCGTGGCACTTTCATCAACAGACTCTGCCCTCAGCTATTTGTTGGCTTTAATTCTCAGGCGAACCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

114

Amino Acids

12.65

Weight (kDa)

9.64

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 57 - 109 2e-06 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 78
AcoI YGGCCR 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 215
AdeI CACNNNGTG 1 cut(s) 35
AfaI GTAC 1 cut(s) 200
AflII CTTAAG 1 cut(s) 230
AgsI TTSAA 2 cut(s) 17, 206
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 2 cut(s) 193, 306
AluI AGCT 2 cut(s) 193, 306
AlwI GGATC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 53, 181
AsuHPI GGTGA 1 cut(s) 93
AxyI CCTNAGG 1 cut(s) 105
BalI TGGCCA 1 cut(s) 211
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 114
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 40, 193
BciT130I CCWGG 1 cut(s) 213
BfaI CTAG 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 230
BisI GCNGC 2 cut(s) 54, 182
BlsI GCNGC 2 cut(s) 55, 183
Bme1390I CCNGG 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 43, 111
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 65
Bpu10I CCTNAGC 1 cut(s) 302
Bse21I CCTNAGG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 213
BseMII CTCAG 3 cut(s) 119, 316, 340
BseXI GCAGC 2 cut(s) 40, 193
BsgI GTGCAG 1 cut(s) 12
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 41
BsnI GGCC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 70, 155
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 3 cut(s) 118, 315, 339
BspLI GGNNCC 2 cut(s) 43, 111
BspPI GGATC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 41
BspTI CTTAAG 1 cut(s) 230
BssMI GATC 2 cut(s) 70, 155
Bst2UI CCWGG 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 105, 302, 326
BstKTI GATC 2 cut(s) 73, 158
BstMBI GATC 2 cut(s) 70, 155
BstMWI GCNNNNNNNGC 2 cut(s) 190, 222
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 40, 193
Bsu36I CCTNAGG 1 cut(s) 105
BsuRI GGCC 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 296
Csp6I GTAC 1 cut(s) 199
CviJI RGCY 7 cut(s) 112, 184, 193, 211, 216, 306, 317
CviKI_1 RGCY 7 cut(s) 112, 184, 193, 211, 216, 306, 317
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 3 cut(s) 105, 302, 326
DpnI GATC 2 cut(s) 72, 157
DpnII GATC 2 cut(s) 70, 155
DraIII CACNNNGTG 1 cut(s) 35
EaeI YGGCCR 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 215
Eco81I CCTNAGG 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 114
FaiI YATR 2 cut(s) 47, 49
FauI CCCGC 1 cut(s) 138
FauNDI CATATG 1 cut(s) 47
Fnu4HI GCNGC 2 cut(s) 54, 182
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 54, 182
FspBI CTAG 1 cut(s) 186
GluI GCNGC 2 cut(s) 54, 182
HaeIII GGCC 1 cut(s) 211
HinfI GANTC 3 cut(s) 13, 98, 293
HphI GGTGA 1 cut(s) 93
Hpy188III TCNNGA 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 60
HpyCH4V TGCA 2 cut(s) 29, 56
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 222
HpyF3I CTNAG 3 cut(s) 105, 302, 326
Kzo9I GATC 2 cut(s) 70, 155
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 5 cut(s) 92, 158, 198, 225, 313
Lsp1109I GCAGC 2 cut(s) 40, 193
LweI GCATC 1 cut(s) 65
MaeI CTAG 1 cut(s) 186
MalI GATC 2 cut(s) 72, 157
MboI GATC 2 cut(s) 70, 155
MboII GAAGA 1 cut(s) 18
MfeI CAATTG 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 3 cut(s) 80, 87, 321
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 1 cut(s) 287
MnlI CCTC 2 cut(s) 114, 311
Mox20I TGGCCA 1 cut(s) 211
MscI TGGCCA 1 cut(s) 211
MseI TTAA 3 cut(s) 122, 231, 320
Msp20I TGGCCA 1 cut(s) 211
MspA1I CMGCKG 1 cut(s) 193
MspCI CTTAAG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 190, 222
NdeI CATATG 1 cut(s) 47
NdeII GATC 2 cut(s) 70, 155
NlaIV GGNNCC 2 cut(s) 43, 111
PfeI GAWTC 2 cut(s) 13, 98
PkrI GCNGC 2 cut(s) 55, 183
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 43, 111
PstNI CAGNNNCTG 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SaqAI TTAA 3 cut(s) 122, 231, 320
SatI GCNGC 2 cut(s) 54, 182
Sau3AI GATC 2 cut(s) 70, 155
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 114
SetI ASST 3 cut(s) 106, 195, 308
SfaNI GCATC 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 3 cut(s) 80, 87, 321
SsiI CCGC 1 cut(s) 145
SspI AATATT 1 cut(s) 20
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 211
TasI AATT 3 cut(s) 80, 87, 321
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 2 cut(s) 13, 98
Tru1I TTAA 3 cut(s) 122, 231, 320
Tru9I TTAA 3 cut(s) 122, 231, 320
TscAI CASTG 1 cut(s) 147
TseI GCWGC 2 cut(s) 53, 181
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 1 cut(s) 147
Vha464I CTTAAG 1 cut(s) 230
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.