Rroxscaffold_7G00189530

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003022
Physical Location & Seq
Forward (+)
29280101 .. 29282010
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189530.1

Sequence Viewer

Length: 543 bp
ATGTCTGATGGGCAATTGTTGTACATGGTTCATGAATTAATTGATGGCGGCTGTTTCACAGTCCTTGGCAACCTGTTCAGGTATCCCACGACTCTCTGTTTCGTTCTCTTGGATATTACTTCATCAGAGTTTTTCCATGTTGTCATGGTCATGACCAAGATCACTAATCACAGTGCCGTTTCAGAAACAAATAGAAGGACTCGTAGAGCAGGGATTCAATGTTTGGCTGTACGTCGGGTTGGCACCACATATGCTGCGTCTGTGGGAAAGGATCATCAGCAGTTGAGTATTGATGATGGAGTACCTCAGGAGCCCTTCCTTCTGAGTCTTTTCAAAGAAATAGTGTGGTCTTTGAGATCATTATTCATTTTTCTGCTTGAGCAGCCAAGTCAGCTGAAGTACATTGAATGGCCAGGTTTCCATAGTACGCTGAAGACTGCGACTCTCACTCTTGTTCTTGTGGCATTGCTCATTGTAGCACTGTCATCAACTGACTCTGCCCTCAGCTATTTGTTGGCTTTGATTCTCAGGAGTAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

180

Amino Acids

20.14

Weight (kDa)

7.77

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 124 - 176 8.5e-06 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AciI CCGC 1 cut(s) 48
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 1 cut(s) 410
AcuI CTGAAG 2 cut(s) 416, 452
AfaI GTAC 5 cut(s) 23, 231, 303, 401, 427
AgsI TTSAA 3 cut(s) 218, 334, 407
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 2 cut(s) 394, 507
AluI AGCT 2 cut(s) 394, 507
AlwI GGATC 1 cut(s) 279
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 2 cut(s) 254, 382
AseI ATTAAT 1 cut(s) 38
AxyI CCTNAGG 1 cut(s) 306
BalI TGGCCA 1 cut(s) 412
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 440
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 241, 394
BccI CCATC 3 cut(s) 2, 38, 290
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 414
BciVI GTATCC 1 cut(s) 93
BfuI GTATCC 1 cut(s) 93
BisI GCNGC 3 cut(s) 49, 255, 383
BlsI GCNGC 3 cut(s) 50, 256, 384
Bme1390I CCNGG 1 cut(s) 414
BmiI GGNNCC 2 cut(s) 244, 312
BmrFI CCNGG 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 440
Bpu10I CCTNAGC 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 64
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 1 cut(s) 464
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 64
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 4 cut(s) 314, 320, 517, 541
BseXI GCAGC 2 cut(s) 241, 394
BshFI GGCC 1 cut(s) 412
BshNI GGYRCC 1 cut(s) 242
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 3 cut(s) 159, 271, 356
BspACI CCGC 1 cut(s) 48
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 4 cut(s) 315, 319, 516, 540
BspHI TCATGA 2 cut(s) 31, 150
BspLI GGNNCC 2 cut(s) 244, 312
BspPI GGATC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 3 cut(s) 159, 271, 356
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 3 cut(s) 61, 173, 483
BstAUI TGTACA 1 cut(s) 21
BstDEI CTNAG 4 cut(s) 306, 323, 503, 527
BstKTI GATC 3 cut(s) 162, 274, 359
BstMBI GATC 3 cut(s) 159, 271, 356
BstMWI GCNNNNNNNGC 2 cut(s) 382, 391
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 241, 394
BstV2I GAAGAC 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 306
BsuI GTATCC 1 cut(s) 93
BsuRI GGCC 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 178, 479
CciI TCATGA 2 cut(s) 31, 150
CseI GACGC 1 cut(s) 246
Csp6I GTAC 5 cut(s) 22, 230, 302, 400, 426
CviAII CATG 5 cut(s) 25, 32, 137, 145, 151
CviJI RGCY 8 cut(s) 51, 227, 313, 385, 394, 412, 507, 518
CviKI_1 RGCY 8 cut(s) 51, 227, 313, 385, 394, 412, 507, 518
CviQI GTAC 5 cut(s) 22, 230, 302, 400, 426
DdeI CTNAG 4 cut(s) 306, 323, 503, 527
DpnI GATC 3 cut(s) 161, 273, 358
DpnII GATC 3 cut(s) 159, 271, 356
EaeI YGGCCR 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 315
Eco57I CTGAAG 2 cut(s) 416, 452
Eco81I CCTNAGG 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 5 cut(s) 28, 35, 140, 148, 154
FaiI YATR 9 cut(s) 26, 33, 138, 146, 152, 250, 252, 423, 541
FatI CATG 5 cut(s) 24, 31, 136, 144, 150
FauNDI CATATG 1 cut(s) 250
Fnu4HI GCNGC 3 cut(s) 49, 255, 383
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 3 cut(s) 49, 255, 383
GluI GCNGC 3 cut(s) 49, 255, 383
HaeIII GGCC 1 cut(s) 412
HgaI GACGC 1 cut(s) 246
Hin1II CATG 5 cut(s) 28, 35, 140, 148, 154
HinfI GANTC 7 cut(s) 91, 199, 214, 325, 442, 494, 523
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 4 cut(s) 7, 127, 184, 324
Hpy188III TCNNGA 4 cut(s) 32, 151, 308, 529
Hpy8I GTNNAC 1 cut(s) 536
Hpy99I CGWCG 1 cut(s) 237
HpyAV CCTTC 3 cut(s) 189, 325, 329
HpyCH4III ACNGT 3 cut(s) 61, 173, 483
HpyCH4IV ACGT 1 cut(s) 232
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 391
HpyF3I CTNAG 4 cut(s) 306, 323, 503, 527
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 5 cut(s) 28, 35, 140, 148, 154
Kzo9I GATC 3 cut(s) 159, 271, 356
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 64, 86, 195, 293, 399, 426, 514
Lsp1109I GCAGC 2 cut(s) 241, 394
MaeII ACGT 1 cut(s) 232
MalI GATC 3 cut(s) 161, 273, 358
MboI GATC 3 cut(s) 159, 271, 356
MboII GAAGA 1 cut(s) 445
MfeI CAATTG 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 412
MluCI AATT 3 cut(s) 14, 35, 39
MluNI TGGCCA 1 cut(s) 412
MlyI GAGTC 5 cut(s) 85, 193, 334, 436, 488
MnlI CCTC 2 cut(s) 315, 512
Mox20I TGGCCA 1 cut(s) 412
MscI TGGCCA 1 cut(s) 412
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 412
MspA1I CMGCKG 1 cut(s) 394
MspR9I CCNGG 1 cut(s) 414
MunI CAATTG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 382, 391
NdeI CATATG 1 cut(s) 250
NdeII GATC 3 cut(s) 159, 271, 356
NlaIII CATG 5 cut(s) 28, 35, 140, 148, 154
NlaIV GGNNCC 2 cut(s) 244, 312
PagI TCATGA 2 cut(s) 31, 150
PfeI GAWTC 2 cut(s) 214, 523
PkrI GCNGC 3 cut(s) 50, 256, 384
PleI GAGTC 5 cut(s) 85, 193, 333, 436, 488
PpsI GAGTC 5 cut(s) 85, 193, 333, 436, 488
PshBI ATTAAT 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 244, 312
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 5 cut(s) 23, 231, 303, 401, 427
RsaNI GTAC 5 cut(s) 22, 230, 302, 400, 426
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 3 cut(s) 49, 255, 383
Sau3AI GATC 3 cut(s) 159, 271, 356
SchI GAGTC 5 cut(s) 85, 193, 334, 436, 488
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 315
SetI ASST 7 cut(s) 75, 83, 235, 307, 396, 418, 509
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 3 cut(s) 14, 35, 39
SsiI CCGC 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 412
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 61, 173, 483
TaiI ACGT 1 cut(s) 235
TasI AATT 3 cut(s) 14, 35, 39
TatI WGTACW 2 cut(s) 21, 399
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 2 cut(s) 214, 523
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 2 cut(s) 178, 486
TseI GCWGC 2 cut(s) 254, 382
TspDTI ATGAA 4 cut(s) 20, 48, 111, 355
TspRI CASTG 2 cut(s) 178, 486
VspI ATTAAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.