FvH4_2g22520

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002074
Physical Location & Seq
Reverse (-)
18473010 .. 18475556
2547 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22520.t1

Sequence Viewer

Length: 753 bp
ATGGAGACAGAGCTCAGGCTAGGGTTGCCCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGTGGTGGTGAAGGAGGAGGAGATCAGGTGGTGGTGATGATGAGGAAGAGAGTCTTCTCTGAAACCGAGAGTCAGATCACTACTGATGATGAGAGCAGTGCTTGTGTGGATTTGAAGCTCAATCTTTCATCCAAGGATGGTAGCAGCACAGCTGAAAAGTCCAAGAGCTTGATGATGAACAAGAACAAGGAGAAGAACGTGGATCTCCCCGCCGATCCGGCAAAGCCTCCGGCCAAGGCACAAGTAGTGGGTTGGCCGCCGGTGAGATCCTTCCGAAAGAACATGTTGAGCGCACAGAAGAGCAGTACTACTGATGAAAGCAAGGCTGGAGGGAATGCAGCTTTGGTGAAGGTGAGCATGGACGGTGCGCCTTACCTCCGGAAAGTCGACCTGAACATGTACAAGACTTACCCTCAGCTCTCTGACGCCCTAGCCAAAATGTTCAGTTCTTTCACCATTGGTAACTGTGGATCCCAAGGCACAATTGATTTCATGAATGAGAGGAAACTGATGGATCTTCTGAATGATTCTGATTACATCCCCACTTATGAAGACAAGGACGGTGACTGGATGCTTGTTGGTGATGTCCCATGGGAGATGTTTGTTGAATCATGCAAGAGACTGCGTATAATGAAGGGCAAAGAAGCTGTTGGACTTGCGCCAAGAGCCATGGAGAAATGCAAGAACAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

251

Amino Acids

27.03

Weight (kDa)

7.55

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 2 - 241 6.8e-84 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 444
AccIII TCCGGA 1 cut(s) 435
AciI CCGC 2 cut(s) 267, 314
AclWI GGATC 6 cut(s) 266, 267, 318, 522, 535, 579
AcoI YGGCCR 2 cut(s) 288, 311
AcyI GRCGYC 1 cut(s) 483
AfaI GTAC 2 cut(s) 364, 458
AfiI CCNNNNNNNGG 1 cut(s) 35
AflIII ACRYGT 2 cut(s) 339, 453
AgsI TTSAA 2 cut(s) 172, 665
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 8 cut(s) 13, 175, 209, 225, 398, 475, 704, 750
AluI AGCT 8 cut(s) 13, 175, 209, 225, 398, 475, 704, 750
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 670
AlwI GGATC 6 cut(s) 266, 267, 318, 522, 535, 579
AlwNI CAGNNNCTG 1 cut(s) 750
Aor13HI TCCGGA 1 cut(s) 435
AoxI GGCC 2 cut(s) 288, 311
ApeKI GCWGC 2 cut(s) 201, 395
AspLEI GCGC 3 cut(s) 350, 427, 718
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 8 cut(s) 77, 103, 331, 415, 421, 502, 632, 650
BamHI GGATCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 2 cut(s) 103, 615
Bbv12I GWGCWC 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 213, 407
BccI CCATC 2 cut(s) 188, 562
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 670
BfaI CTAG 2 cut(s) 20, 488
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 3 cut(s) 202, 314, 396
BlsI GCNGC 3 cut(s) 203, 315, 397
BmcAI AGTACT 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 618
BpiI GAAGAC 2 cut(s) 103, 615
BpmI CTGGAG 1 cut(s) 405
Bpu10I CCTNAGC 2 cut(s) 14, 471
BpuMI CCSGG 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 483
BsaJI CCNNGG 5 cut(s) 189, 291, 532, 647, 726
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 629
BseAI TCCGGA 1 cut(s) 435
BseDI CCNNGG 5 cut(s) 189, 291, 532, 647, 726
BseGI GGATG 4 cut(s) 185, 199, 594, 633
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 28, 485
BseNI ACTGG 1 cut(s) 629
BseXI GCAGC 2 cut(s) 213, 407
BshFI GGCC 2 cut(s) 290, 313
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 5 cut(s) 30, 275, 287, 317, 436
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 670
BsmFI GGGAC 1 cut(s) 629
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 290, 313
Bsp1286I GDGCHC 1 cut(s) 15
Bsp13I TCCGGA 1 cut(s) 435
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
Bsp19I CCATGG 2 cut(s) 647, 726
BspACI CCGC 2 cut(s) 267, 314
BspANI GGCC 2 cut(s) 290, 313
BspCNI CTCAG 2 cut(s) 27, 484
BspEI TCCGGA 1 cut(s) 435
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 1 cut(s) 529
BspPI GGATC 6 cut(s) 266, 267, 318, 522, 535, 579
BspQI GCTCTTC 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 1 cut(s) 629
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 5 cut(s) 189, 291, 532, 647, 726
BssMI GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
BssNI GRCGYC 1 cut(s) 483
BssT1I CCWWGG 5 cut(s) 189, 291, 532, 647, 726
Bst4CI ACNGT 3 cut(s) 422, 524, 620
Bst6I CTCTTC 2 cut(s) 98, 350
BstACI GRCGYC 1 cut(s) 483
BstAUI TGTACA 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 14, 471
BstDSI CCRYGG 2 cut(s) 647, 726
BstF5I GGATG 4 cut(s) 185, 199, 594, 633
BstHHI GCGC 3 cut(s) 350, 427, 718
BstKTI GATC 7 cut(s) 82, 135, 262, 274, 326, 530, 574
BstMAI GTCTC 1 cut(s) 670
BstMBI GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
BstMWI GCNNNNNNNGC 3 cut(s) 25, 275, 722
BstNSI RCATGY 2 cut(s) 343, 457
BstSCI CCNGG 1 cut(s) 28
BstV1I GCAGC 2 cut(s) 213, 407
BstV2I GAAGAC 2 cut(s) 103, 615
BstX2I RGATCY 4 cut(s) 259, 323, 527, 571
BstYI RGATCY 4 cut(s) 259, 323, 527, 571
BsuRI GGCC 2 cut(s) 290, 313
BtgI CCRYGG 2 cut(s) 647, 726
BtsCI GGATG 4 cut(s) 185, 199, 594, 633
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 160
CaiI CAGNNNCTG 1 cut(s) 750
CciI TCATGA 1 cut(s) 549
CfoI GCGC 3 cut(s) 350, 427, 718
Cfr10I RCCGGY 1 cut(s) 316
CseI GACGC 1 cut(s) 491
Csp6I GTAC 2 cut(s) 363, 457
CviAII CATG 7 cut(s) 340, 415, 454, 550, 648, 669, 727
CviQI GTAC 2 cut(s) 363, 457
DdeI CTNAG 2 cut(s) 14, 471
DpnI GATC 7 cut(s) 81, 134, 261, 273, 325, 529, 573
DpnII GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
EaeI YGGCCR 2 cut(s) 288, 311
Eam1104I CTCTTC 2 cut(s) 98, 350
EarI CTCTTC 2 cut(s) 98, 350
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 5 cut(s) 189, 291, 532, 647, 726
Eco24I GRGCYC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 13
EcoT14I CCWWGG 5 cut(s) 189, 291, 532, 647, 726
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 5 cut(s) 189, 291, 532, 647, 726
FaeI CATG 7 cut(s) 343, 418, 457, 553, 651, 672, 730
FaiI YATR 9 cut(s) 341, 416, 455, 551, 606, 649, 670, 686, 728
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 1 cut(s) 629
FatI CATG 7 cut(s) 339, 414, 453, 549, 647, 668, 726
FauI CCCGC 1 cut(s) 274
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 3 cut(s) 202, 314, 396
FokI GGATG 4 cut(s) 172, 206, 581, 640
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 3 cut(s) 202, 314, 396
FspBI CTAG 2 cut(s) 20, 488
GlaI GCGC 3 cut(s) 349, 426, 717
GluI GCNGC 3 cut(s) 202, 314, 396
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 2 cut(s) 290, 313
HapII CCGG 5 cut(s) 30, 275, 287, 317, 436
HgaI GACGC 1 cut(s) 491
HhaI GCGC 3 cut(s) 350, 427, 718
Hin1I GRCGYC 1 cut(s) 483
Hin1II CATG 7 cut(s) 343, 418, 457, 553, 651, 672, 730
Hin6I GCGC 3 cut(s) 348, 425, 716
HinP1I GCGC 3 cut(s) 348, 425, 716
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 4 cut(s) 108, 127, 584, 665
HpaII CCGG 5 cut(s) 30, 275, 287, 317, 436
HphI GGTGA 8 cut(s) 77, 103, 331, 415, 421, 502, 632, 650
Hpy166II GTNNAC 1 cut(s) 445
Hpy188I TCNGA 6 cut(s) 118, 132, 332, 481, 579, 589
Hpy188III TCNNGA 2 cut(s) 436, 550
Hpy8I GTNNAC 1 cut(s) 445
HpyAV CCTTC 4 cut(s) 62, 337, 400, 685
HpyCH4III ACNGT 3 cut(s) 422, 524, 620
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 3 cut(s) 395, 672, 738
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 275, 722
HpyF3I CTNAG 2 cut(s) 14, 471
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 483
Hsp92II CATG 7 cut(s) 343, 418, 457, 553, 651, 672, 730
HspAI GCGC 3 cut(s) 348, 425, 716
Kpn2I TCCGGA 1 cut(s) 435
Kzo9I GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
LguI GCTCTTC 1 cut(s) 350
LpnPI CCDG 9 cut(s) 43, 68, 288, 300, 330, 369, 449, 461, 610
Lsp1109I GCAGC 2 cut(s) 213, 407
LweI GCATC 1 cut(s) 618
MaeI CTAG 2 cut(s) 20, 488
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 518, 620
MalI GATC 7 cut(s) 81, 134, 261, 273, 325, 529, 573
MboI GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
MboII GAAGA 6 cut(s) 103, 115, 262, 367, 566, 620
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 4 cut(s) 259, 323, 527, 571
MhlI GDGCHC 1 cut(s) 15
MluCI AATT 1 cut(s) 540
MlyI GAGTC 2 cut(s) 117, 136
MmeI TCCRAC 1 cut(s) 688
MroI TCCGGA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 5 cut(s) 30, 275, 287, 317, 436
MspR9I CCNGG 1 cut(s) 30
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 1 cut(s) 397
MwoI GCNNNNNNNGC 3 cut(s) 25, 275, 722
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 2 cut(s) 647, 726
NdeII GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
NlaIII CATG 7 cut(s) 343, 418, 457, 553, 651, 672, 730
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 620
NspI RCATGY 2 cut(s) 343, 457
PagI TCATGA 1 cut(s) 549
PciI ACATGT 2 cut(s) 339, 453
PciSI GCTCTTC 1 cut(s) 350
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 2 cut(s) 584, 665
PkrI GCNGC 3 cut(s) 203, 315, 397
PleI GAGTC 2 cut(s) 116, 135
PpsI GAGTC 2 cut(s) 116, 135
PscI ACATGT 2 cut(s) 339, 453
Psp124BI GAGCTC 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 750
PsuI RGATCY 4 cut(s) 259, 323, 527, 571
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 364, 458
RsaNI GTAC 2 cut(s) 363, 457
SacI GAGCTC 1 cut(s) 15
SalI GTCGAC 1 cut(s) 443
SapI GCTCTTC 1 cut(s) 350
SatI GCNGC 3 cut(s) 202, 314, 396
Sau3AI GATC 7 cut(s) 79, 132, 259, 271, 323, 527, 571
ScaI AGTACT 1 cut(s) 364
SchI GAGTC 2 cut(s) 117, 136
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 15
SfaNI GCATC 1 cut(s) 618
SgrAI CRCCGGYG 1 cut(s) 316
Sse9I AATT 1 cut(s) 540
SsiI CCGC 2 cut(s) 267, 314
SspMI CTAG 2 cut(s) 20, 488
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 5 cut(s) 189, 291, 532, 647, 726
TaaI ACNGT 3 cut(s) 422, 524, 620
TaiI ACGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 444
TasI AATT 1 cut(s) 540
TatI WGTACW 2 cut(s) 362, 456
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 2 cut(s) 584, 665
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 2 cut(s) 201, 395
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 7 cut(s) 174, 248, 387, 538, 566, 621, 704
TspRI CASTG 1 cut(s) 160
XceI RCATGY 2 cut(s) 343, 457
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 2 cut(s) 20, 488
ZrmI AGTACT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.