Rh6BG322600

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002074
Physical Location & Seq
Reverse (-)
55388376 .. 55390503
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG322600.1

Sequence Viewer

Length: 738 bp
ATGGAGACAGAGCTCAGGCTAGGGTTGCCCGGAGGAGGAGGAGGAGGTGGTGAAGGAGAAGATCAGGTGGTGGTGATGATGAGGAAGAGAGGCTTCTCTGAAACCGAGAGTGATATCACTACTGATGATGAGAGCAGCACTTGTGTGGATTTGAAGCTGAAACTTTCTTCCAAGGATGGTAGCAGCAGCACTAATGAAAAGTCCAAGAGCTTGATGATGAACAAGAACAAGGAGAAGAACATGGATCTTCCCGCCGATCCGGCAAAGCCTCCGGCCAAGGCACAAGTAGTGGGTTGGCCGCCGGTGAGATCCTTCCGGAAGAACATGTTGAGCGCACAGAAGAGCAGTTCTACTGATGAAAGCAAGGCTGGAGGAAATGCAGCTTTGGTGAAGGTGAGCATGGATGGTGCGCCTTACCTCCGAAAGGTCGACCTGAACATGTACAAGACTTACCCTCAGCTCTCCGACGCCCTAGCCAAAATGTTCAGCTCGTTCACCATTGGTAACTGTGGATCCCAAGGAACGATTGATTTCATGAATGAGAGGAAGCTGATGGATCTTCTCAATGATTCTGATTACATCCCAACTTATGAAGACAAGGACGGTGACTGGATGCTTGTTGGTGATGTCCCGTGGGAGATGTTTGTTGAATCATGCAAGAGACTGCGCATAATGAAGGGAAAGGAGGCTGTTGGACTCGCACCAAGAGCCATGGAGAAATGCAAGAACAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

245

Amino Acids

26.89

Weight (kDa)

6.45

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 2 - 236 5.5e-85 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 668
AccI GTMKAC 1 cut(s) 429
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 2 cut(s) 252, 299
AclWI GGATC 6 cut(s) 251, 252, 303, 507, 520, 564
AcoI YGGCCR 2 cut(s) 273, 296
AcyI GRCGYC 1 cut(s) 468
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 2 cut(s) 35, 424
AflIII ACRYGT 2 cut(s) 324, 438
AgsI TTSAA 2 cut(s) 154, 650
AleI CACNNNNGTG 1 cut(s) 143
AluBI AGCT 8 cut(s) 13, 157, 210, 383, 460, 489, 550, 735
AluI AGCT 8 cut(s) 13, 157, 210, 383, 460, 489, 550, 735
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 655
AlwI GGATC 6 cut(s) 251, 252, 303, 507, 520, 564
AlwNI CAGNNNCTG 1 cut(s) 735
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 2 cut(s) 273, 296
ApeKI GCWGC 4 cut(s) 135, 183, 186, 380
AspLEI GCGC 3 cut(s) 335, 412, 669
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 8 cut(s) 62, 85, 316, 400, 406, 487, 617, 635
BamHI GGATCC 1 cut(s) 512
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 4 cut(s) 147, 195, 198, 392
BccI CCATC 3 cut(s) 170, 398, 547
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 655
BfaI CTAG 2 cut(s) 20, 473
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 5 cut(s) 136, 184, 187, 299, 381
BlsI GCNGC 5 cut(s) 137, 185, 188, 300, 382
Bme1390I CCNGG 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 603
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 2 cut(s) 14, 456
BpuMI CCSGG 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 468
BsaJI CCNNGG 5 cut(s) 171, 276, 517, 632, 711
BsaWI WCCGGW 1 cut(s) 315
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 424
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 614
BseAI TCCGGA 1 cut(s) 315
BseDI CCNNGG 5 cut(s) 171, 276, 517, 632, 711
BseGI GGATG 4 cut(s) 181, 409, 579, 618
BseLI CCNNNNNNNGG 2 cut(s) 35, 424
BseMII CTCAG 2 cut(s) 28, 470
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 4 cut(s) 48, 51, 54, 57
BseXI GCAGC 4 cut(s) 147, 195, 198, 392
BshFI GGCC 2 cut(s) 275, 298
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 5 cut(s) 30, 260, 272, 302, 316
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 2 cut(s) 35, 424
BsmAI GTCTC 1 cut(s) 655
BsmFI GGGAC 1 cut(s) 614
BsnI GGCC 2 cut(s) 275, 298
Bsp1286I GDGCHC 1 cut(s) 15
Bsp13I TCCGGA 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 6 cut(s) 61, 244, 256, 308, 512, 556
Bsp19I CCATGG 1 cut(s) 711
BspACI CCGC 2 cut(s) 252, 299
BspANI GGCC 2 cut(s) 275, 298
BspCNI CTCAG 2 cut(s) 27, 469
BspEI TCCGGA 1 cut(s) 315
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 1 cut(s) 514
BspPI GGATC 6 cut(s) 251, 252, 303, 507, 520, 564
BspQI GCTCTTC 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 1 cut(s) 614
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 5 cut(s) 171, 276, 517, 632, 711
BssMI GATC 6 cut(s) 61, 244, 256, 308, 512, 556
BssNI GRCGYC 1 cut(s) 468
BssT1I CCWWGG 4 cut(s) 171, 276, 517, 711
Bst4CI ACNGT 2 cut(s) 509, 605
Bst6I CTCTTC 2 cut(s) 80, 335
BstACI GRCGYC 1 cut(s) 468
BstAUI TGTACA 1 cut(s) 441
BstDEI CTNAG 2 cut(s) 14, 456
BstDSI CCRYGG 2 cut(s) 632, 711
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 4 cut(s) 181, 409, 579, 618
BstHHI GCGC 3 cut(s) 335, 412, 669
BstKTI GATC 6 cut(s) 64, 247, 259, 311, 515, 559
BstMAI GTCTC 1 cut(s) 655
BstMBI GATC 6 cut(s) 61, 244, 256, 308, 512, 556
BstMWI GCNNNNNNNGC 3 cut(s) 25, 260, 707
BstNSI RCATGY 2 cut(s) 328, 442
BstSCI CCNGG 1 cut(s) 28
BstV1I GCAGC 4 cut(s) 147, 195, 198, 392
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 4 cut(s) 244, 308, 512, 556
BstYI RGATCY 4 cut(s) 244, 308, 512, 556
BsuRI GGCC 2 cut(s) 275, 298
BtgI CCRYGG 2 cut(s) 632, 711
BtsCI GGATG 4 cut(s) 181, 409, 579, 618
CaiI CAGNNNCTG 1 cut(s) 735
CciI TCATGA 1 cut(s) 534
CfoI GCGC 3 cut(s) 335, 412, 669
Cfr10I RCCGGY 1 cut(s) 301
CseI GACGC 1 cut(s) 476
Csp6I GTAC 1 cut(s) 442
CviAII CATG 7 cut(s) 241, 325, 400, 439, 535, 654, 712
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 14, 456
DpnI GATC 6 cut(s) 63, 246, 258, 310, 514, 558
DpnII GATC 6 cut(s) 61, 244, 256, 308, 512, 556
EaeI YGGCCR 2 cut(s) 273, 296
Eam1104I CTCTTC 2 cut(s) 80, 335
EarI CTCTTC 2 cut(s) 80, 335
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 4 cut(s) 171, 276, 517, 711
Eco24I GRGCYC 1 cut(s) 15
Eco32I GATATC 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 4 cut(s) 171, 276, 517, 711
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 4 cut(s) 171, 276, 517, 711
FaeI CATG 7 cut(s) 244, 328, 403, 442, 538, 657, 715
FaiI YATR 9 cut(s) 242, 326, 401, 440, 536, 591, 655, 671, 713
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 614
FatI CATG 7 cut(s) 240, 324, 399, 438, 534, 653, 711
FauI CCCGC 1 cut(s) 259
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 5 cut(s) 136, 184, 187, 299, 381
FokI GGATG 4 cut(s) 188, 416, 566, 625
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 5 cut(s) 136, 184, 187, 299, 381
FspBI CTAG 2 cut(s) 20, 473
FspI TGCGCA 1 cut(s) 668
GlaI GCGC 3 cut(s) 334, 411, 668
GluI GCNGC 5 cut(s) 136, 184, 187, 299, 381
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 2 cut(s) 275, 298
HapII CCGG 5 cut(s) 30, 260, 272, 302, 316
HgaI GACGC 1 cut(s) 476
HhaI GCGC 3 cut(s) 335, 412, 669
Hin1I GRCGYC 1 cut(s) 468
Hin1II CATG 7 cut(s) 244, 328, 403, 442, 538, 657, 715
Hin6I GCGC 3 cut(s) 333, 410, 667
HinP1I GCGC 3 cut(s) 333, 410, 667
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 3 cut(s) 569, 650, 696
HpaII CCGG 5 cut(s) 30, 260, 272, 302, 316
HphI GGTGA 8 cut(s) 62, 85, 316, 400, 406, 487, 617, 635
Hpy166II GTNNAC 2 cut(s) 430, 495
Hpy188I TCNGA 4 cut(s) 100, 422, 466, 574
Hpy188III TCNNGA 2 cut(s) 316, 535
Hpy8I GTNNAC 2 cut(s) 430, 495
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 4 cut(s) 47, 322, 385, 670
HpyCH4III ACNGT 2 cut(s) 509, 605
HpyCH4V TGCA 3 cut(s) 380, 657, 723
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 260, 707
HpyF3I CTNAG 2 cut(s) 14, 456
Hsp92I GRCGYC 1 cut(s) 468
Hsp92II CATG 7 cut(s) 244, 328, 403, 442, 538, 657, 715
HspAI GCGC 3 cut(s) 333, 410, 667
Kpn2I TCCGGA 1 cut(s) 315
Kzo9I GATC 6 cut(s) 61, 244, 256, 308, 512, 556
LguI GCTCTTC 1 cut(s) 335
LpnPI CCDG 9 cut(s) 43, 50, 273, 285, 315, 329, 354, 446, 595
Lsp1109I GCAGC 4 cut(s) 147, 195, 198, 392
LweI GCATC 1 cut(s) 603
MaeI CTAG 2 cut(s) 20, 473
MaeIII GTNAC 2 cut(s) 503, 605
MalI GATC 6 cut(s) 63, 246, 258, 310, 514, 558
MboI GATC 6 cut(s) 61, 244, 256, 308, 512, 556
MboII GAAGA 9 cut(s) 71, 97, 159, 239, 247, 331, 352, 551, 605
MflI RGATCY 4 cut(s) 244, 308, 512, 556
MhlI GDGCHC 1 cut(s) 15
MlyI GAGTC 1 cut(s) 690
MmeI TCCRAC 2 cut(s) 489, 673
MroI TCCGGA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 5 cut(s) 30, 260, 272, 302, 316
MspR9I CCNGG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 25, 260, 707
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 711
NdeII GATC 6 cut(s) 61, 244, 256, 308, 512, 556
NlaIII CATG 7 cut(s) 244, 328, 403, 442, 538, 657, 715
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 1 cut(s) 605
NsbI TGCGCA 1 cut(s) 668
NspI RCATGY 2 cut(s) 328, 442
OliI CACNNNNGTG 1 cut(s) 143
PagI TCATGA 1 cut(s) 534
PciI ACATGT 2 cut(s) 324, 438
PciSI GCTCTTC 1 cut(s) 335
PfeI GAWTC 2 cut(s) 569, 650
PkrI GCNGC 5 cut(s) 137, 185, 188, 300, 382
PleI GAGTC 1 cut(s) 690
PpsI GAGTC 1 cut(s) 690
PscI ACATGT 2 cut(s) 324, 438
Psp124BI GAGCTC 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 735
PsuI RGATCY 4 cut(s) 244, 308, 512, 556
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 15
SalI GTCGAC 1 cut(s) 428
SapI GCTCTTC 1 cut(s) 335
SatI GCNGC 5 cut(s) 136, 184, 187, 299, 381
Sau3AI GATC 6 cut(s) 61, 244, 256, 308, 512, 556
SchI GAGTC 1 cut(s) 690
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 15
SfaNI GCATC 1 cut(s) 603
SgrAI CRCCGGYG 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 143
SsiI CCGC 2 cut(s) 252, 299
SspMI CTAG 2 cut(s) 20, 473
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 4 cut(s) 171, 276, 517, 711
TaaI ACNGT 2 cut(s) 509, 605
TaqI TCGA 1 cut(s) 429
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 2 cut(s) 569, 650
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 4 cut(s) 135, 183, 186, 380
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 7 cut(s) 210, 233, 372, 523, 551, 606, 689
XagI CCTNNNNNAGG 1 cut(s) 422
XceI RCATGY 2 cut(s) 328, 442
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 2 cut(s) 20, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.