MD10G1192900.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002074
Physical Location & Seq
Reverse (-)
28909337 .. 28912808
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1192900.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGGGTTCGAAGAGACAGAGCTGAGGCTAGGGTTGCCGGGTGGTGGTGGCGGCGGCGTCCGTGATGGAGATCAGGTGATCGTGATGAGGAAGAGAGGGTTTTCTGAAACTGAGAGTGATATTAGTACTGATGCTAGTACTTGTGTGGATTTGAAGCTTAATCTTTCTAATAATTCTAAGGAGACAAATAGTACTGGTGGGAAAGATGGCAGTGCTGAAAAGTCCAAGACCAACAAGGAGAAGAACAACAACCTTGATTTTCGGGCTTCCGATCCAGCAAAGCCTCCTGCCGCCAAGGCACAAGTTGTGGGTTGGCCACCGGTGCGATCTTTTCGAAAGAACATGTTCACGGCGGTGCAAAAGAGCACGAATGATGGAGAAAGTGAGCAGATGAACAAGGGCAGCAATAATAATGCAGTTTTGGTGAAGGTTAGCATGGATGGTGCACCATACCTTCGCAAGGTCGACTTGAAGATGTACAAGAGTTACCCTGAGCTCTCTGATGCCCTAGCCAAAATGTTCAGCTCCTTCACCATTGGAAATTGTGGATCCCAAGGAATGAAGGACTTCATGAATGAGAGAAAGCTGATGGATGTTCTCAACGGTTCTGATTACATTCCAACATACGAAGACAAGGATGGTGATTGGATGCTGGTTGGCGATGTGCCATGGGAGATGTTTGTTGAATCATGCAAGAGATTGCGCATAATGAAGAGCAAGGAGGCTGTTGGACTAGCACCAAGGGCCATGGAGAAATGCAAGAACAGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

258

Amino Acids

28.29

Weight (kDa)

8.53

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 5 - 248 4.7e-88 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 704
AccI GTMKAC 1 cut(s) 465
AciI CCGC 4 cut(s) 51, 54, 291, 353
AclWI GGATC 3 cut(s) 266, 543, 556
AcoI YGGCCR 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 4 cut(s) 127, 139, 193, 479
AfiI CCNNNNNNNGG 2 cut(s) 44, 460
AflIII ACRYGT 1 cut(s) 342
AgeI ACCGGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 154, 472, 686
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 6 cut(s) 22, 157, 496, 525, 586, 771
AluI AGCT 6 cut(s) 22, 157, 496, 525, 586, 771
Alw21I GWGCWC 3 cut(s) 368, 448, 498
Alw26I GTCTC 2 cut(s) 8, 176
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 3 cut(s) 266, 543, 556
AlwNI CAGNNNCTG 1 cut(s) 771
AoxI GGCC 2 cut(s) 314, 744
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 1 cut(s) 402
AsiGI ACCGGT 1 cut(s) 319
Asp700I GAANNNNTTC 2 cut(s) 344, 566
AspLEI GCGC 1 cut(s) 705
AspS9I GGNCC 1 cut(s) 744
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 4 cut(s) 88, 436, 523, 653
AsuII TTCGAA 2 cut(s) 9, 334
BaeGI GKGCMC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 316
BamHI GGATCC 1 cut(s) 548
BanII GRGCYC 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 3 cut(s) 368, 448, 498
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 414
BccI CCATC 6 cut(s) 59, 200, 368, 434, 583, 632
BceAI ACGGC 1 cut(s) 366
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 8, 176
BfaI CTAG 4 cut(s) 29, 135, 509, 734
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 4 cut(s) 52, 55, 291, 403
BlsI GCNGC 4 cut(s) 53, 56, 292, 404
BmcAI AGTACT 3 cut(s) 127, 139, 193
Bme1390I CCNGG 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 744
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 3 cut(s) 121, 493, 639
BpiI GAAGAC 1 cut(s) 636
Bpu10I CCTNAGC 2 cut(s) 23, 492
Bpu14I TTCGAA 2 cut(s) 9, 334
BpuMI CCSGG 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 5 cut(s) 294, 553, 668, 740, 747
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 460
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 199
Bse8I GATNNNNATC 1 cut(s) 69
BseDI CCNNGG 5 cut(s) 294, 553, 668, 740, 747
BseGI GGATG 4 cut(s) 445, 598, 643, 654
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 44, 460
BseMII CTCAG 3 cut(s) 14, 102, 483
BseNI ACTGG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 1 cut(s) 414
BshFI GGCC 2 cut(s) 316, 746
BshTI ACCGGT 1 cut(s) 319
BsiHKAI GWGCWC 3 cut(s) 368, 448, 498
BsiSI CCGG 2 cut(s) 38, 320
BslI CCNNNNNNNGG 2 cut(s) 44, 460
BsmAI GTCTC 2 cut(s) 8, 176
BsnI GGCC 2 cut(s) 316, 746
Bsp119I TTCGAA 2 cut(s) 9, 334
Bsp1286I GDGCHC 3 cut(s) 368, 448, 498
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 5 cut(s) 70, 78, 271, 326, 548
Bsp19I CCATGG 2 cut(s) 668, 747
BspACI CCGC 4 cut(s) 51, 54, 291, 353
BspANI GGCC 2 cut(s) 316, 746
BspCNI CTCAG 3 cut(s) 15, 103, 484
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 3 cut(s) 266, 543, 556
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 2 cut(s) 9, 334
BsrFI RCCGGY 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 5 cut(s) 294, 553, 668, 740, 747
BssMI GATC 5 cut(s) 70, 78, 271, 326, 548
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 5 cut(s) 294, 553, 668, 740, 747
Bst4CI ACNGT 1 cut(s) 605
Bst6I CTCTTC 3 cut(s) 6, 86, 707
BstACI GRCGYC 1 cut(s) 57
BstAUI TGTACA 1 cut(s) 477
BstBI TTCGAA 2 cut(s) 9, 334
BstDEI CTNAG 4 cut(s) 23, 111, 177, 492
BstDSI CCRYGG 2 cut(s) 668, 747
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 4 cut(s) 445, 598, 643, 654
BstHHI GCGC 1 cut(s) 705
BstKTI GATC 5 cut(s) 73, 81, 274, 329, 551
BstMAI GTCTC 2 cut(s) 8, 176
BstMBI GATC 5 cut(s) 70, 78, 271, 326, 548
BstMWI GCNNNNNNNGC 4 cut(s) 34, 296, 322, 743
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 414
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
BsuRI GGCC 2 cut(s) 316, 746
BtgI CCRYGG 2 cut(s) 668, 747
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 4 cut(s) 445, 598, 643, 654
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 217
CaiI CAGNNNCTG 1 cut(s) 771
CciI TCATGA 1 cut(s) 570
CfoI GCGC 1 cut(s) 705
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 744
CseI GACGC 1 cut(s) 46
Csp6I GTAC 4 cut(s) 126, 138, 192, 478
CspAI ACCGGT 1 cut(s) 319
CviAII CATG 6 cut(s) 343, 436, 571, 669, 690, 748
CviQI GTAC 4 cut(s) 126, 138, 192, 478
DdeI CTNAG 4 cut(s) 23, 111, 177, 492
DpnI GATC 5 cut(s) 72, 80, 273, 328, 550
DpnII GATC 5 cut(s) 70, 78, 271, 326, 548
EaeI YGGCCR 1 cut(s) 314
Eam1104I CTCTTC 3 cut(s) 6, 86, 707
EarI CTCTTC 3 cut(s) 6, 86, 707
Ecl136II GAGCTC 1 cut(s) 496
Eco130I CCWWGG 5 cut(s) 294, 553, 668, 740, 747
Eco24I GRGCYC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 496
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoT14I CCWWGG 5 cut(s) 294, 553, 668, 740, 747
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 5 cut(s) 294, 553, 668, 740, 747
FaeI CATG 6 cut(s) 346, 439, 574, 672, 693, 751
FaiI YATR 9 cut(s) 344, 437, 451, 572, 625, 670, 691, 707, 749
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FatI CATG 6 cut(s) 342, 435, 570, 668, 689, 747
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 52, 55, 291, 403
FokI GGATG 4 cut(s) 452, 605, 650, 661
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 4 cut(s) 52, 55, 291, 403
FspBI CTAG 4 cut(s) 29, 135, 509, 734
FspI TGCGCA 1 cut(s) 704
GlaI GCGC 1 cut(s) 704
GluI GCNGC 4 cut(s) 52, 55, 291, 403
HaeIII GGCC 2 cut(s) 316, 746
HapII CCGG 2 cut(s) 38, 320
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 705
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 6 cut(s) 346, 439, 574, 672, 693, 751
Hin6I GCGC 1 cut(s) 703
HinP1I GCGC 1 cut(s) 703
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 686
HpaII CCGG 2 cut(s) 38, 320
HphI GGTGA 4 cut(s) 88, 436, 523, 653
Hpy166II GTNNAC 3 cut(s) 348, 446, 466
Hpy188I TCNGA 4 cut(s) 106, 271, 502, 610
Hpy188III TCNNGA 2 cut(s) 82, 571
Hpy8I GTNNAC 3 cut(s) 348, 446, 466
HpyAV CCTTC 4 cut(s) 421, 464, 538, 556
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4V TGCA 5 cut(s) 358, 416, 446, 693, 759
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 296, 322, 743
HpyF3I CTNAG 4 cut(s) 23, 111, 177, 492
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 6 cut(s) 346, 439, 574, 672, 693, 751
HspAI GCGC 1 cut(s) 703
Kzo9I GATC 5 cut(s) 70, 78, 271, 326, 548
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 2 cut(s) 530, 768
LpnPI CCDG 9 cut(s) 51, 59, 180, 288, 300, 333, 504, 638, 751
Lsp1109I GCAGC 1 cut(s) 414
LweI GCATC 3 cut(s) 121, 493, 639
MaeI CTAG 4 cut(s) 29, 135, 509, 734
MaeIII GTNAC 1 cut(s) 485
MalI GATC 5 cut(s) 72, 80, 273, 328, 550
MboI GATC 5 cut(s) 70, 78, 271, 326, 548
MboII GAAGA 6 cut(s) 23, 103, 253, 484, 641, 724
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 3 cut(s) 368, 448, 498
MlsI TGGCCA 1 cut(s) 316
MluCI AATT 2 cut(s) 172, 541
MluNI TGGCCA 1 cut(s) 316
MmeI TCCRAC 2 cut(s) 644, 709
MnlI CCTC 5 cut(s) 18, 81, 89, 294, 715
Mox20I TGGCCA 1 cut(s) 316
MroXI GAANNNNTTC 2 cut(s) 344, 566
MscI TGGCCA 1 cut(s) 316
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 316
MspI CCGG 2 cut(s) 38, 320
MspR9I CCNGG 1 cut(s) 39
MwoI GCNNNNNNNGC 4 cut(s) 34, 296, 322, 743
NciI CCSGG 1 cut(s) 39
NcoI CCATGG 2 cut(s) 668, 747
NdeII GATC 5 cut(s) 70, 78, 271, 326, 548
NlaIII CATG 6 cut(s) 346, 439, 574, 672, 693, 751
NlaIV GGNNCC 1 cut(s) 550
NsbI TGCGCA 1 cut(s) 704
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 2 cut(s) 9, 334
OliI CACNNNNGTG 1 cut(s) 353
PagI TCATGA 1 cut(s) 570
PciI ACATGT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 707
PdmI GAANNNNTTC 2 cut(s) 344, 566
PfeI GAWTC 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 319
PkrI GCNGC 4 cut(s) 53, 56, 292, 404
PscI ACATGT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 498
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 1 cut(s) 744
PstNI CAGNNNCTG 1 cut(s) 771
PsuI RGATCY 1 cut(s) 548
RsaI GTAC 4 cut(s) 127, 139, 193, 479
RsaNI GTAC 4 cut(s) 126, 138, 192, 478
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 498
SalI GTCGAC 1 cut(s) 464
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 4 cut(s) 52, 55, 291, 403
Sau3AI GATC 5 cut(s) 70, 78, 271, 326, 548
Sau96I GGNCC 1 cut(s) 744
ScaI AGTACT 3 cut(s) 127, 139, 193
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 3 cut(s) 368, 448, 498
SfaNI GCATC 3 cut(s) 121, 493, 639
SfuI TTCGAA 2 cut(s) 9, 334
SgrAI CRCCGGYG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 353
Sse9I AATT 2 cut(s) 172, 541
SsiI CCGC 4 cut(s) 51, 54, 291, 353
SspMI CTAG 4 cut(s) 29, 135, 509, 734
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 5 cut(s) 294, 553, 668, 740, 747
TaaI ACNGT 1 cut(s) 605
TaqI TCGA 3 cut(s) 9, 334, 465
TasI AATT 2 cut(s) 172, 541
TatI WGTACW 4 cut(s) 125, 137, 191, 477
TauI GCSGC 3 cut(s) 54, 57, 293
TfiI GAWTC 1 cut(s) 686
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 217
TseI GCWGC 1 cut(s) 402
TspDTI ATGAA 5 cut(s) 407, 559, 575, 587, 725
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 444
XagI CCTNNNNNAGG 1 cut(s) 458
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 2 cut(s) 344, 566
XspI CTAG 4 cut(s) 29, 135, 509, 734
ZrmI AGTACT 3 cut(s) 127, 139, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.