MD05G1205800.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002074
Physical Location & Seq
Reverse (-)
33498233 .. 33502458
4226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1205800.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGGGTTTGAAGAGACAGAGCTGAGGCTAGGGCTGCCGGGTGGTGGTGGTGGCGGTGGCCGTGACGGAGATCAGGTGGTGGTGATGAGGAAGAGAGGGTTTTCGGAAACTGAGAGTAAGATTAGTACTGATACTAGTACTTGTGTGGATTTGAAGCTTAATCTTTCTAATAGTTCTAAGGAGGCAAATAGTACTGGTGGGAAAGATGGCATTGCTGTTAAGTCCAAGACGAACAAGGAGAAGAACAACCACCTTGATCTTCCGGCTTCCGATCCAGCAAAGCCTCCTGCTGCCAAGGCACAAGTCGTGGGTTGGCCACCGGTGCGTTCTTTCCGAAAGAACATGTTCACGGGGGTGCAAAAGAGCTCAAATGACGGAGAAAGTGAGCAGATGAACAAGGGTGGCAATAATAATGCAGTTCTGGTGAAGGTTAGCATGGATGGTGCACCCTACCTTCGCAAGGTCGACTTGAAGATGTACAAGAGTTACCCTGAGCTCTCTGATGCCCTAGCCAAAATGTTCAGCTCCTTCACCATTGGAAATTGTGGATCCCAAGGAATGAAGGACTTCATGAATGAGAGCAAGCTGATGGATGTTCTCAACGGTTCTGATTACACTCCAACATATGAAGACAAGGATGGTGATTGGATGCTGGTTGGCGATGTCCCGTGGGAGATGTTTGTTGAATCATGCAAGAGATTGCGTATAATGAAGAGCAAGGAGGCTGTTGGGCTCGCACCAAGAGCCATGGAGAAATGCAAGAACAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

258

Amino Acids

28.0

Weight (kDa)

8.73

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 5 - 248 2.9e-87 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 465
AciI CCGC 1 cut(s) 54
AclWI GGATC 3 cut(s) 266, 543, 556
AcoI YGGCCR 2 cut(s) 58, 314
AfaI GTAC 4 cut(s) 127, 139, 193, 479
AfiI CCNNNNNNNGG 2 cut(s) 44, 460
AflIII ACRYGT 1 cut(s) 342
AgeI ACCGGT 1 cut(s) 319
AgsI TTSAA 4 cut(s) 11, 154, 472, 686
AhlI ACTAGT 1 cut(s) 134
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 7 cut(s) 22, 157, 366, 496, 525, 586, 771
AluI AGCT 7 cut(s) 22, 157, 366, 496, 525, 586, 771
Alw21I GWGCWC 3 cut(s) 368, 448, 498
Alw26I GTCTC 1 cut(s) 8
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 3 cut(s) 266, 543, 556
AlwNI CAGNNNCTG 1 cut(s) 771
AoxI GGCC 2 cut(s) 58, 314
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 34, 290
AsiGI ACCGGT 1 cut(s) 319
Asp700I GAANNNNTTC 2 cut(s) 344, 566
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 4 cut(s) 94, 436, 523, 653
BaeGI GKGCMC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 316
BamHI GGATCC 1 cut(s) 548
BanII GRGCYC 3 cut(s) 368, 498, 735
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 3 cut(s) 368, 448, 498
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 21, 277
BccI CCATC 4 cut(s) 200, 434, 583, 632
BceAI ACGGC 1 cut(s) 45
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 8
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 3 cut(s) 29, 135, 509
BisI GCNGC 2 cut(s) 35, 291
BlsI GCNGC 2 cut(s) 36, 292
BmcAI AGTACT 3 cut(s) 127, 139, 193
Bme1390I CCNGG 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 2 cut(s) 493, 639
BpiI GAAGAC 1 cut(s) 636
Bpu10I CCTNAGC 2 cut(s) 23, 492
BpuMI CCSGG 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 294, 553, 668, 747
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 460
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 210
BseDI CCNNGG 4 cut(s) 294, 553, 668, 747
BseGI GGATG 4 cut(s) 445, 598, 643, 654
BseLI CCNNNNNNNGG 2 cut(s) 44, 460
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 3 cut(s) 14, 102, 483
BseNI ACTGG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 2 cut(s) 21, 277
BshFI GGCC 2 cut(s) 60, 316
BshTI ACCGGT 1 cut(s) 319
BsiHKAI GWGCWC 3 cut(s) 368, 448, 498
BsiSI CCGG 3 cut(s) 38, 263, 320
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 2 cut(s) 44, 460
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 2 cut(s) 60, 316
Bsp1286I GDGCHC 4 cut(s) 368, 448, 498, 735
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 4 cut(s) 70, 256, 271, 548
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 1 cut(s) 54
BspANI GGCC 2 cut(s) 60, 316
BspCNI CTCAG 3 cut(s) 15, 103, 484
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 3 cut(s) 266, 543, 556
BspQI GCTCTTC 1 cut(s) 707
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 4 cut(s) 294, 553, 668, 747
BssMI GATC 4 cut(s) 70, 256, 271, 548
BssT1I CCWWGG 3 cut(s) 294, 553, 747
Bst4CI ACNGT 1 cut(s) 605
Bst6I CTCTTC 3 cut(s) 6, 86, 707
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 584, 735
BstDEI CTNAG 4 cut(s) 23, 111, 177, 492
BstDSI CCRYGG 2 cut(s) 668, 747
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 4 cut(s) 445, 598, 643, 654
BstKTI GATC 4 cut(s) 73, 259, 274, 551
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 4 cut(s) 70, 256, 271, 548
BstMWI GCNNNNNNNGC 4 cut(s) 34, 296, 322, 743
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 21, 277
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
BsuRI GGCC 2 cut(s) 60, 316
BtgI CCRYGG 2 cut(s) 668, 747
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 4 cut(s) 445, 598, 643, 654
Cac8I GCNNGC 2 cut(s) 584, 735
CaiI CAGNNNCTG 1 cut(s) 771
CciI TCATGA 1 cut(s) 570
Cfr10I RCCGGY 1 cut(s) 319
Csp6I GTAC 4 cut(s) 126, 138, 192, 478
CspAI ACCGGT 1 cut(s) 319
CviAII CATG 5 cut(s) 343, 436, 571, 690, 748
CviQI GTAC 4 cut(s) 126, 138, 192, 478
DdeI CTNAG 4 cut(s) 23, 111, 177, 492
DpnI GATC 4 cut(s) 72, 258, 273, 550
DpnII GATC 4 cut(s) 70, 256, 271, 548
EaeI YGGCCR 2 cut(s) 58, 314
Eam1104I CTCTTC 3 cut(s) 6, 86, 707
EarI CTCTTC 3 cut(s) 6, 86, 707
Ecl136II GAGCTC 2 cut(s) 366, 496
Eco130I CCWWGG 3 cut(s) 294, 553, 747
Eco24I GRGCYC 3 cut(s) 368, 498, 735
Eco53kI GAGCTC 2 cut(s) 366, 496
EcoICRI GAGCTC 2 cut(s) 366, 496
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoT14I CCWWGG 3 cut(s) 294, 553, 747
EcoT38I GRGCYC 3 cut(s) 368, 498, 735
ErhI CCWWGG 3 cut(s) 294, 553, 747
FaeI CATG 5 cut(s) 346, 439, 574, 693, 751
FaiI YATR 8 cut(s) 344, 437, 572, 625, 627, 691, 707, 749
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 1 cut(s) 650
FatI CATG 5 cut(s) 342, 435, 570, 689, 747
FauNDI CATATG 1 cut(s) 625
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 35, 291
FokI GGATG 4 cut(s) 452, 605, 650, 661
FriOI GRGCYC 3 cut(s) 368, 498, 735
Fsp4HI GCNGC 2 cut(s) 35, 291
FspBI CTAG 3 cut(s) 29, 135, 509
GluI GCNGC 2 cut(s) 35, 291
HaeIII GGCC 2 cut(s) 60, 316
HapII CCGG 3 cut(s) 38, 263, 320
Hin1II CATG 5 cut(s) 346, 439, 574, 693, 751
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 686
HpaII CCGG 3 cut(s) 38, 263, 320
HphI GGTGA 4 cut(s) 94, 436, 523, 653
Hpy166II GTNNAC 3 cut(s) 348, 446, 466
Hpy188I TCNGA 5 cut(s) 106, 271, 335, 502, 610
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 3 cut(s) 348, 446, 466
HpyAV CCTTC 4 cut(s) 421, 464, 538, 556
HpyCH4III ACNGT 1 cut(s) 605
HpyCH4V TGCA 5 cut(s) 358, 416, 446, 693, 759
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 296, 322, 743
HpyF3I CTNAG 4 cut(s) 23, 111, 177, 492
Hsp92II CATG 5 cut(s) 346, 439, 574, 693, 751
Kzo9I GATC 4 cut(s) 70, 256, 271, 548
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 1 cut(s) 530
Lsp1109I GCAGC 2 cut(s) 21, 277
LweI GCATC 2 cut(s) 493, 639
MaeI CTAG 3 cut(s) 29, 135, 509
MaeIII GTNAC 2 cut(s) 62, 485
MalI GATC 4 cut(s) 72, 258, 273, 550
MboI GATC 4 cut(s) 70, 256, 271, 548
MboII GAAGA 7 cut(s) 23, 103, 251, 253, 484, 641, 724
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 4 cut(s) 368, 448, 498, 735
MlsI TGGCCA 1 cut(s) 316
MluCI AATT 1 cut(s) 541
MluNI TGGCCA 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 6 cut(s) 18, 81, 89, 175, 294, 715
Mox20I TGGCCA 1 cut(s) 316
MroXI GAANNNNTTC 2 cut(s) 344, 566
MscI TGGCCA 1 cut(s) 316
MseI TTAA 2 cut(s) 159, 219
MslI CAYNNNNRTG 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 316
MspI CCGG 3 cut(s) 38, 263, 320
MspR9I CCNGG 1 cut(s) 39
MwoI GCNNNNNNNGC 4 cut(s) 34, 296, 322, 743
NciI CCSGG 1 cut(s) 39
NcoI CCATGG 1 cut(s) 747
NdeI CATATG 1 cut(s) 625
NdeII GATC 4 cut(s) 70, 256, 271, 548
NlaIII CATG 5 cut(s) 346, 439, 574, 693, 751
NlaIV GGNNCC 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 62
NspI RCATGY 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 353
PagI TCATGA 1 cut(s) 570
PciI ACATGT 1 cut(s) 342
PciSI GCTCTTC 1 cut(s) 707
PdmI GAANNNNTTC 2 cut(s) 344, 566
PfeI GAWTC 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 319
PkrI GCNGC 2 cut(s) 36, 292
PscI ACATGT 1 cut(s) 342
Psp124BI GAGCTC 2 cut(s) 368, 498
PspN4I GGNNCC 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 771
PsuI RGATCY 1 cut(s) 548
RsaI GTAC 4 cut(s) 127, 139, 193, 479
RsaNI GTAC 4 cut(s) 126, 138, 192, 478
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 2 cut(s) 368, 498
SalI GTCGAC 1 cut(s) 464
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 2 cut(s) 159, 219
SatI GCNGC 2 cut(s) 35, 291
Sau3AI GATC 4 cut(s) 70, 256, 271, 548
ScaI AGTACT 3 cut(s) 127, 139, 193
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 4 cut(s) 368, 448, 498, 735
SfaNI GCATC 2 cut(s) 493, 639
SgrAI CRCCGGYG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 353
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 1 cut(s) 541
SsiI CCGC 1 cut(s) 54
SspMI CTAG 3 cut(s) 29, 135, 509
SstI GAGCTC 2 cut(s) 368, 498
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 3 cut(s) 294, 553, 747
TaaI ACNGT 1 cut(s) 605
TaqI TCGA 1 cut(s) 465
TasI AATT 1 cut(s) 541
TatI WGTACW 4 cut(s) 125, 137, 191, 477
TfiI GAWTC 1 cut(s) 686
Tru1I TTAA 2 cut(s) 159, 219
Tru9I TTAA 2 cut(s) 159, 219
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 2 cut(s) 34, 290
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 6 cut(s) 407, 559, 575, 587, 642, 725
TspGWI ACGGA 2 cut(s) 81, 390
VneI GTGCAC 1 cut(s) 444
XagI CCTNNNNNAGG 1 cut(s) 458
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 2 cut(s) 344, 566
XspI CTAG 3 cut(s) 29, 135, 509
ZrmI AGTACT 3 cut(s) 127, 139, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.