FvH4_2g28750

cysteine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000957
Physical Location & Seq
Forward (+)
22459968 .. 22460412
445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28750.t1

Sequence Viewer

Length: 342 bp
ATGCACCGTTTCACCAACCTCAAACGGAGAATCCGTTGCCTCAGCGCCACGACTAATTCAACCAATTCTGTTGCCGGCGAATCGGAGATGCCGGCGTCGTCGTCGGAGCTCTGGCTGCACAACACAATGAGCAGGAAGAAGGAGCTGTTTAAGCCCAAAGTTGAAGGCAGAGTCGGCATGTATGTGTGTGGCGTTACGGCTTACGATCTCAGGCATATTGGCCACGCTCGCGTCTACGTCACTTTCGACGTTCTCTACAGATATCTTAGGCATTTGGGATATGAGGTCACCTATGTTCGAAATTTCACAGACGTTGATGACAAAGTGAGTTCTTATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

114

Amino Acids

13.12

Weight (kDa)

9.51

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 50 - 109 9e-25 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 234
AccII CGCG 1 cut(s) 231
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 1 cut(s) 301
AcyI GRCGYC 1 cut(s) 95
AgsI TTSAA 2 cut(s) 60, 164
AluBI AGCT 2 cut(s) 109, 145
AluI AGCT 2 cut(s) 109, 145
Alw21I GWGCWC 1 cut(s) 111
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 1 cut(s) 301
AspLEI GCGC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 4, 280
AsuII TTCGAA 1 cut(s) 298
BalI TGGCCA 1 cut(s) 222
BanII GRGCYC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 111
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 102
BceAI ACGGC 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 256
BfoI RGCGCY 1 cut(s) 48
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
BmsI GCATC 1 cut(s) 78
Bpu10I CCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bse118I RCCGGY 2 cut(s) 74, 91
BseMII CTCAG 2 cut(s) 55, 223
BseXI GCAGC 1 cut(s) 102
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 2 cut(s) 75, 92
BsnI GGCC 1 cut(s) 222
Bsp119I TTCGAA 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 205
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 2 cut(s) 54, 222
BspFNI CGCG 1 cut(s) 231
BspT104I TTCGAA 1 cut(s) 298
BsrFI RCCGGY 2 cut(s) 74, 91
BssAI RCCGGY 2 cut(s) 74, 91
BssMI GATC 1 cut(s) 205
BssNI GRCGYC 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 8
BstACI GRCGYC 1 cut(s) 95
BstBI TTCGAA 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 76, 93, 229
BstDEI CTNAG 3 cut(s) 41, 209, 266
BstEII GGTNACC 1 cut(s) 286
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 1 cut(s) 208
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 4 cut(s) 115, 151, 174, 228
BstNSI RCATGY 1 cut(s) 181
BstPI GGTNACC 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 256
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 102
BsuRI GGCC 1 cut(s) 222
Cac8I GCNNGC 3 cut(s) 76, 93, 229
CfoI GCGC 1 cut(s) 47
Cfr10I RCCGGY 2 cut(s) 74, 91
CseI GACGC 2 cut(s) 84, 220
CviAII CATG 1 cut(s) 178
CviJI RGCY 6 cut(s) 109, 115, 145, 154, 200, 222
CviKI_1 RGCY 6 cut(s) 109, 115, 145, 154, 200, 222
DdeI CTNAG 3 cut(s) 41, 209, 266
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 111
Eco32I GATATC 1 cut(s) 263
Eco53kI GAGCTC 1 cut(s) 109
Eco91I GGTNACC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 109
EcoO65I GGTNACC 1 cut(s) 286
EcoRV GATATC 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 1 cut(s) 181
FaiI YATR 6 cut(s) 179, 183, 216, 282, 294, 336
FatI CATG 1 cut(s) 177
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 116
GlaI GCGC 1 cut(s) 46
GluI GCNGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 1 cut(s) 222
HapII CCGG 2 cut(s) 75, 92
HgaI GACGC 2 cut(s) 84, 220
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HinfI GANTC 3 cut(s) 30, 80, 171
HpaII CCGG 2 cut(s) 75, 92
HphI GGTGA 2 cut(s) 4, 280
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 2 cut(s) 85, 106
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 4 cut(s) 100, 103, 106, 251
HpyAV CCTTC 2 cut(s) 133, 158
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4IV ACGT 3 cut(s) 237, 249, 312
HpyCH4V TGCA 2 cut(s) 4, 118
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 151, 174, 228
HpyF3I CTNAG 3 cut(s) 41, 209, 266
HpySE526I ACGT 3 cut(s) 237, 249, 312
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 1 cut(s) 45
KroI GCCGGC 2 cut(s) 74, 91
KroNI GCCGGC 2 cut(s) 76, 93
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 106, 142
LpnPI CCDG 5 cut(s) 88, 97, 105, 118, 196
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 78
MaeII ACGT 3 cut(s) 237, 249, 312
MaeIII GTNAC 3 cut(s) 193, 238, 286
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 3 cut(s) 55, 64, 301
MluNI TGGCCA 1 cut(s) 222
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 3 cut(s) 29, 50, 277
Mox20I TGGCCA 1 cut(s) 222
MroNI GCCGGC 2 cut(s) 74, 91
MscI TGGCCA 1 cut(s) 222
MseI TTAA 2 cut(s) 150, 340
MslI CAYNNNNRTG 1 cut(s) 182
Msp20I TGGCCA 1 cut(s) 222
MspI CCGG 2 cut(s) 75, 92
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 4 cut(s) 115, 151, 174, 228
NaeI GCCGGC 2 cut(s) 76, 93
NdeII GATC 1 cut(s) 205
NgoMIV GCCGGC 2 cut(s) 74, 91
NlaIII CATG 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 238, 286
NspI RCATGY 1 cut(s) 181
NspV TTCGAA 1 cut(s) 298
PcsI WCGNNNNNNNCGW 2 cut(s) 31, 243
PdiI GCCGGC 2 cut(s) 76, 93
PfeI GAWTC 2 cut(s) 30, 80
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 182
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 2 cut(s) 150, 340
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 1 cut(s) 205
SchI GAGTC 1 cut(s) 180
SduI GDGCHC 1 cut(s) 111
SetI ASST 8 cut(s) 21, 111, 147, 240, 252, 288, 293, 315
SfaNI GCATC 1 cut(s) 78
SfcI CTRYAG 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 182
Sse9I AATT 3 cut(s) 55, 64, 301
SstI GAGCTC 1 cut(s) 111
TaaI ACNGT 1 cut(s) 8
TaiI ACGT 3 cut(s) 240, 252, 315
TaqI TCGA 2 cut(s) 246, 298
TasI AATT 3 cut(s) 55, 64, 301
TfiI GAWTC 2 cut(s) 30, 80
Tru1I TTAA 2 cut(s) 150, 340
Tru9I TTAA 2 cut(s) 150, 340
TseFI GTSAC 2 cut(s) 238, 286
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 2 cut(s) 238, 286
TspGWI ACGGA 2 cut(s) 23, 40
XapI RAATTY 1 cut(s) 301
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.