Rh5DG245300

cysteine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000957
Physical Location & Seq
Reverse (-)
28022617 .. 28024704
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG245300.1

Sequence Viewer

Length: 399 bp
ATGGGCACTGGAAGACCTGGTTGGCATATCGAGTGCAGTGCCATGAGTACTACTTATCTGGGTTACTCTTTTGACATACATGGTGGAGGGATGGACCTTCTGTTTCCCCCACATGAGAATGAAATTGCTCAGAGTTGTGCTACTTGTAAACAAAGTTATATAAGCTACTGGATACACAATGGTAAAGTGACTCGGTCACCAGCTCGATTATCCTCTATGTATTCTGTGAAGTGCAGTTGTTGGTTTGATATCCACATGAACATTGATAAATTCCCTCTAGTGAAATATCCTGTGCTGGTAATACATGGTTATGCGAACAATTTGATGCAAGGGAGAAGTTACAGCAGTCAATGGAGGATTGTTCAGCATCATTGGGAGTATTCAATGTCTAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

132

Amino Acids

15.16

Weight (kDa)

8.29

Isoelectric Point (pI)

77.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 2 - 71 2.6e-30 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 269
AfaI GTAC 1 cut(s) 49
AgsI TTSAA 1 cut(s) 384
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 165, 203
AluI AGCT 2 cut(s) 165, 203
ApoI RAATTY 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 1 cut(s) 189
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 19
BccI CCATC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 165
BfaI CTAG 2 cut(s) 278, 390
BfuI GTATCC 1 cut(s) 165
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 2 cut(s) 315, 376
BpiI GAAGAC 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 13, 173
BseBI CCWGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 13, 173
BseSI GKGCMC 1 cut(s) 8
BsgI GTGCAG 2 cut(s) 55, 253
Bsp1286I GDGCHC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 142
BsrI ACTGG 2 cut(s) 13, 173
Bst2UI CCWGG 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 195
BstF5I GGATG 1 cut(s) 96
BstNI CCWGG 1 cut(s) 18
BstPI GGTNACC 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 19
BsuI GTATCC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 6, 43
Cfr13I GGNCC 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 16
Csp6I GTAC 1 cut(s) 48
CviAII CATG 5 cut(s) 43, 80, 113, 256, 305
CviJI RGCY 2 cut(s) 165, 203
CviKI_1 RGCY 2 cut(s) 165, 203
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 129
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 94
Eco91I GGTNACC 1 cut(s) 195
EcoO65I GGTNACC 1 cut(s) 195
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 250
FaeI CATG 5 cut(s) 46, 83, 116, 259, 308
FatI CATG 5 cut(s) 42, 79, 112, 255, 304
FokI GGATG 1 cut(s) 103
FspBI CTAG 2 cut(s) 278, 390
Hin1II CATG 5 cut(s) 46, 83, 116, 259, 308
HinfI GANTC 1 cut(s) 190
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 132
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 36, 234, 328
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 5 cut(s) 46, 83, 116, 259, 308
LpnPI CCDG 7 cut(s) 3, 30, 44, 154, 213, 281, 303
LweI GCATC 2 cut(s) 315, 376
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 2 cut(s) 278, 390
MaeIII GTNAC 4 cut(s) 62, 187, 195, 338
MboII GAAGA 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 4 cut(s) 123, 269, 319, 394
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 4 cut(s) 80, 223, 285, 348
MslI CAYNNNNRTG 2 cut(s) 117, 309
MspR9I CCNGG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 18
NlaIII CATG 5 cut(s) 46, 83, 116, 259, 308
NmuCI GTSAC 2 cut(s) 187, 195
PflFI GACNNNGTC 1 cut(s) 193
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 16
PspEI GGTNACC 1 cut(s) 195
PspGI CCWGG 1 cut(s) 16
PspPI GGNCC 1 cut(s) 94
PsyI GACNNNGTC 1 cut(s) 193
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 2 cut(s) 117, 309
Sau96I GGNCC 1 cut(s) 94
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 1 cut(s) 8
SetI ASST 4 cut(s) 19, 99, 167, 205
SexAI ACCWGGT 1 cut(s) 16
SfaNI GCATC 2 cut(s) 315, 376
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 117, 309
Sse9I AATT 4 cut(s) 123, 269, 319, 394
SspMI CTAG 2 cut(s) 278, 390
StyD4I CCNGG 1 cut(s) 16
TaqI TCGA 2 cut(s) 30, 205
TaqII GACCGA 1 cut(s) 183
TasI AATT 4 cut(s) 123, 269, 319, 394
TatI WGTACW 1 cut(s) 47
TscAI CASTG 2 cut(s) 13, 43
TseFI GTSAC 2 cut(s) 187, 195
Tsp45I GTSAC 2 cut(s) 187, 195
TspDTI ATGAA 2 cut(s) 135, 272
TspRI CASTG 2 cut(s) 13, 43
Tth111I GACNNNGTC 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 1 cut(s) 269
XspI CTAG 2 cut(s) 278, 390
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.