Rh5BG237700

DALR_2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000957
Physical Location & Seq
Reverse (-)
28349251 .. 28352624
3374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG237700.1

Sequence Viewer

Length: 384 bp
ATGGGCACTGGAAGACCTGGTTGGCATATCGAGTGCAGTGCCATGAGTACTACTTATCTGGGTTACTCTTTTGACATACATGGTGGAGGGATGGACCTTCTGTTTCCCCCACATGAGAATGAAATTGCTCAGAGTTGTGCTGCTTGTAAACAAAGTTATATAAGCTACTGGATACACAATGGTTTTGTCACTATAGACTCTGAAAAAATGTTCAAATCTCTATGGAACTTCTTTACAATTCGACAGCAAGGAGAAAAGCTTAACCCACGCGGTATTGCAAACTTTTTAGAGTTTAGACCTCTCAAAAGCCAGCTAGATCATCGACCAGAATCTGTATCGATCACTAGCTTGATTAGGCAGATATTAGCAGGTACGATTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

127

Amino Acids

14.44

Weight (kDa)

7.78

Isoelectric Point (pI)

62.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 2 - 112 2e-42 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 359
AccII CGCG 1 cut(s) 270
AciI CCGC 1 cut(s) 270
AfaI GTAC 2 cut(s) 49, 373
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 4 cut(s) 165, 259, 313, 348
AluI AGCT 4 cut(s) 165, 259, 313, 348
AlwNI CAGNNNCTG 1 cut(s) 332
ApeKI GCWGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 94
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 127
BccI CCATC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 165
BfaI CTAG 2 cut(s) 314, 345
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 1 cut(s) 359
BfuI GTATCC 1 cut(s) 165
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmrFI CCNGG 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 19
Bsa29I ATCGAT 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 13, 173
BseBI CCWGG 1 cut(s) 18
BseCI ATCGAT 1 cut(s) 338
BseGI GGATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 13, 173
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 270
BshVI ATCGAT 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 316, 339
BspACI CCGC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 142
BspDI ATCGAT 1 cut(s) 338
BspFNI CGCG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 359
BsrI ACTGG 2 cut(s) 13, 173
BssMI GATC 2 cut(s) 316, 339
Bst2UI CCWGG 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 311
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 270
BstKTI GATC 2 cut(s) 319, 342
BstMBI GATC 2 cut(s) 316, 339
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 338
BsuI GTATCC 1 cut(s) 165
BsuTUI ATCGAT 1 cut(s) 338
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 6, 43
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 311
CaiI CAGNNNCTG 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 338
CsiI ACCWGGT 1 cut(s) 16
Csp6I GTAC 2 cut(s) 48, 372
CviAII CATG 3 cut(s) 43, 80, 113
CviJI RGCY 5 cut(s) 165, 259, 309, 313, 348
CviKI_1 RGCY 5 cut(s) 165, 259, 309, 313, 348
CviQI GTAC 2 cut(s) 48, 372
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 318, 341
DpnII GATC 2 cut(s) 316, 339
Eco47I GGWCC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 16
FaeI CATG 3 cut(s) 46, 83, 116
FaiI YATR 9 cut(s) 27, 44, 77, 81, 114, 159, 161, 194, 223
FatI CATG 3 cut(s) 42, 79, 112
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 2 cut(s) 314, 345
GluI GCNGC 1 cut(s) 141
Hin1II CATG 3 cut(s) 46, 83, 116
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 3 cut(s) 197, 329, 376
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 2 cut(s) 132, 202
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 1 cut(s) 107
HpyCH4V TGCA 2 cut(s) 36, 278
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 3 cut(s) 46, 83, 116
Kzo9I GATC 2 cut(s) 316, 339
LpnPI CCDG 7 cut(s) 3, 30, 44, 154, 323, 339, 354
Lsp1109I GCAGC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 2 cut(s) 314, 345
MaeIII GTNAC 2 cut(s) 62, 187
MalI GATC 2 cut(s) 318, 341
MboI GATC 2 cut(s) 316, 339
MboII GAAGA 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 123, 237
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 2 cut(s) 80, 309
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 270
NdeII GATC 2 cut(s) 316, 339
NlaIII CATG 3 cut(s) 46, 83, 116
NmuCI GTSAC 1 cut(s) 187
PfeI GAWTC 2 cut(s) 329, 376
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspPI GGNCC 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 49, 373
RsaNI GTAC 2 cut(s) 48, 372
RseI CAYNNNNRTG 1 cut(s) 117
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 2 cut(s) 316, 339
Sau96I GGNCC 1 cut(s) 94
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 1 cut(s) 8
SetI ASST 8 cut(s) 19, 99, 167, 261, 301, 315, 350, 373
SexAI ACCWGGT 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 117
Sse9I AATT 2 cut(s) 123, 237
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 314, 345
StyD4I CCNGG 1 cut(s) 16
TaqI TCGA 4 cut(s) 30, 241, 322, 338
TasI AATT 2 cut(s) 123, 237
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 2 cut(s) 329, 376
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 13, 43
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 135
TspRI CASTG 2 cut(s) 13, 43
VpaK11BI GGWCC 1 cut(s) 94
XspI CTAG 2 cut(s) 314, 345
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.