Rh4DG323500

cysteine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000957
Physical Location & Seq
Forward (+)
54814788 .. 54816724
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG323500.1

Sequence Viewer

Length: 342 bp
ATGGGCACTGGAAGACCTGGTTGGCATATCGAGTGCAGTGCCATGAGTACTGCTTATCTGGGTTACTCTTTTGACATACATGGTGGAGGGATGGACCTTCTGTTTCCCCCACATGAGAATGAAATTGCTCAGAGTTGTGCTGCTTGTAAACAAAGTTATATAAGCTACTGGATACACAATGGTTTTGTCACTATAGACTCTGAAAAAATGTTCAAATCACTATGGAACTTTTTTACAATTCGACAGGTACAAGTGGTCACAATTTTTTCTTCTAAATTGTTGGGAACCATCATTCTAATGGTTGAGTATCTAGCTTTAGAGTCAAATCCTTGTCTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

113

Amino Acids

12.72

Weight (kDa)

5.69

Isoelectric Point (pI)

64.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 2 - 103 2.3e-41 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 2 cut(s) 49, 249
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 165, 314
AluI AGCT 2 cut(s) 165, 314
ApeKI GCWGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 94
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 85, 296
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 165
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 165
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 286
BmrFI CCNGG 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 13, 173
BseBI CCWGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 13, 173
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 55
Bsp1286I GDGCHC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 286
BsrI ACTGG 2 cut(s) 13, 173
Bst2UI CCWGG 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 96
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 19
BsuI GTATCC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 6, 43
Cfr13I GGNCC 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 16
Csp6I GTAC 2 cut(s) 48, 248
CviAII CATG 3 cut(s) 43, 80, 113
CviJI RGCY 2 cut(s) 165, 314
CviKI_1 RGCY 2 cut(s) 165, 314
CviQI GTAC 2 cut(s) 48, 248
DdeI CTNAG 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 16
FaeI CATG 3 cut(s) 46, 83, 116
FaiI YATR 9 cut(s) 27, 44, 77, 81, 114, 159, 161, 194, 223
FatI CATG 3 cut(s) 42, 79, 112
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 311
GluI GCNGC 1 cut(s) 141
Hin1II CATG 3 cut(s) 46, 83, 116
HinfI GANTC 2 cut(s) 197, 320
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 3 cut(s) 132, 202, 336
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 1 cut(s) 107
HpyCH4V TGCA 1 cut(s) 36
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 3 cut(s) 46, 83, 116
LpnPI CCDG 5 cut(s) 3, 30, 44, 154, 230
Lsp1109I GCAGC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 1 cut(s) 311
MaeIII GTNAC 3 cut(s) 62, 187, 256
MboII GAAGA 2 cut(s) 24, 261
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 4 cut(s) 123, 237, 261, 275
MlyI GAGTC 2 cut(s) 191, 329
MnlI CCTC 1 cut(s) 80
MseI TTAA 1 cut(s) 340
MslI CAYNNNNRTG 2 cut(s) 117, 296
MspR9I CCNGG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 18
NlaIII CATG 3 cut(s) 46, 83, 116
NlaIV GGNNCC 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 187, 256
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 2 cut(s) 191, 328
PpsI GAGTC 2 cut(s) 191, 328
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 1 cut(s) 94
RsaI GTAC 2 cut(s) 49, 249
RsaNI GTAC 2 cut(s) 48, 248
RseI CAYNNNNRTG 2 cut(s) 117, 296
SaqAI TTAA 1 cut(s) 340
SatI GCNGC 1 cut(s) 141
Sau96I GGNCC 1 cut(s) 94
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 2 cut(s) 191, 329
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 1 cut(s) 8
SetI ASST 5 cut(s) 19, 99, 167, 249, 316
SexAI ACCWGGT 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 117, 296
Sse9I AATT 4 cut(s) 123, 237, 261, 275
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 16
TaqI TCGA 2 cut(s) 30, 241
TasI AATT 4 cut(s) 123, 237, 261, 275
TatI WGTACW 1 cut(s) 47
Tru1I TTAA 1 cut(s) 340
Tru9I TTAA 1 cut(s) 340
TscAI CASTG 2 cut(s) 13, 43
TseFI GTSAC 2 cut(s) 187, 256
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 187, 256
TspDTI ATGAA 1 cut(s) 135
TspRI CASTG 2 cut(s) 13, 43
VpaK11BI GGWCC 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XspI CTAG 1 cut(s) 311
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.