Rh6DG384600

cysteine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000957
Physical Location & Seq
Forward (+)
57777032 .. 57780739
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG384600.1

Sequence Viewer

Length: 1206 bp
ATGGAGTATCTTCATTGCCTGCCTCCTTCTGTGGAACCGCGAGTGTCCGATCACATGCCACAGATCATTGATATGATTAAGCAGATTATTGAAAATGGACATGCCTATGGAGTTGATGGGGATGTCTATTTCAGTGTTGACAAGTTCCCAGATTATGGACGTCTATCTGGGCGAAAATTAGAAGATAATCGAGCCGGTGAGCGAGTCACTGTTGATTCAAGGAAGAAAAATCCTGCTGATTTTGCTTTATGGAAGTCTGCAAAGGAAGGTGAACCCTTCTGGGACAGTCCATGGGGCCCTGGAAGACCTGGTTGGCATATCGAGTGCAGTGCCATGAGTGCTGCTTATTTGGGTTACTCTTTTGACATACATGGTGGAGGGATGGACCTTGTGTTTCCCCACCATGAGAATGAAATTGCTCAGAGTTGTGCTGCATGTAAACAAAGTGATATAAGCTACTGGATACACAATGGTTTTGTCACTATAGACTCCGAGAAAATGTCGAAATCCCTAGGGAACTTCTTTACAATTCGGCAGGTTATAGACCTATACCATCCATTGGCTTTGCGACTTTTCTTATTAGGGACCCATTATCAGTCTCCAATCAACTACTCTGATGTGCAGCTTGAAAGCACTTCAGAGCGGATCTTCTACATATTTCAGACTTTATATGATTGTGAAAGTGTCGTAAGCCAGCATGATGTGTCAACCCTGAAGGATACCATTCCTCCTGAAATAGTGGAAAGCATCAATAAGTTCTACAATGTTTTTGTAACATCAATGTCGGATGATCTTCACAGTTCTGTTGTTTTTGGTGCATTATCAGACCCATTAAAAACTATGAATGATCTGCTACACACTCGCAAGGGCAAGAAGCAAGCATTGAGGATAGAGTCTCTTGCAGCTTTGGAAAAGATTACCAGGAATGTTTTAAGCATTTTGGGATTACTGCCTGCAAGTTACGCCGAGATTCTGCAGCAGCTGAGAGAAAAAGCCTTGAAGCGTGCAAAATTAACAGAAGATCAAGTATTGCAGAAAATCGAGGACAGAACTGCAGCAAGGAAGAACAAAGAGTACGAAAAATCAGATACAATTCGAAAAAATTTAGCTGCTGTGGGAATCACTCTTATGGACAACCCAGATGGGACAACATGGAGACCAGCTGTACCTCTTGCAATTCAAGAGCAGCAGGTGGCATCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

401

Amino Acids

45.28

Weight (kDa)

5.83

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 1 - 212 3e-92 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1180
AatII GACGTC 1 cut(s) 163
Acc36I ACCTGC 2 cut(s) 526, 1180
AccB7I CCANNNNNTGG 2 cut(s) 155, 559
AccBSI CCGCTC 1 cut(s) 643
AccII CGCG 1 cut(s) 40
AciI CCGC 2 cut(s) 38, 643
AclWI GGATC 1 cut(s) 653
AcsI RAATTY 1 cut(s) 1102
AcuI CTGAAG 2 cut(s) 621, 734
AcyI GRCGYC 1 cut(s) 160
AfaI GTAC 2 cut(s) 1076, 1167
AfiI CCNNNNNNNGG 2 cut(s) 155, 559
AgsI TTSAA 5 cut(s) 92, 219, 629, 1000, 1181
AjnI CCWGG 3 cut(s) 298, 307, 920
AjuI GAANNNNNNNTTGG 2 cut(s) 295, 327
AluBI AGCT 6 cut(s) 456, 625, 905, 982, 1109, 1163
AluI AGCT 6 cut(s) 456, 625, 905, 982, 1109, 1163
Alw26I GTCTC 3 cut(s) 603, 900, 1150
AlwI GGATC 1 cut(s) 653
AlwNI CAGNNNCTG 1 cut(s) 982
AoxI GGCC 1 cut(s) 295
ApaI GGGCCC 1 cut(s) 299
ApeKI GCWGC 9 cut(s) 341, 431, 622, 902, 976, 979, 1055, 1109, 1186
ApoI RAATTY 1 cut(s) 1102
AspA2I CCTAGG 1 cut(s) 511
AspS9I GGNCC 4 cut(s) 295, 296, 385, 585
AsuHPI GGTGA 2 cut(s) 209, 281
AsuII TTCGAA 1 cut(s) 1096
AvaII GGWCC 2 cut(s) 385, 585
AvrII CCTAGG 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 310
BbvI GCAGC 9 cut(s) 328, 418, 634, 914, 988, 991, 1067, 1096, 1198
BccI CCATC 4 cut(s) 110, 376, 561, 1136
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 3 cut(s) 300, 309, 922
BciVI GTATCC 2 cut(s) 456, 712
BcoDI GTCTC 3 cut(s) 603, 900, 1150
BfaI CTAG 1 cut(s) 512
BfmI CTRYAG 3 cut(s) 483, 974, 1053
BfuAI ACCTGC 2 cut(s) 526, 1180
BfuI GTATCC 2 cut(s) 456, 712
BisI GCNGC 9 cut(s) 342, 432, 623, 903, 977, 980, 1056, 1110, 1187
BlnI CCTAGG 1 cut(s) 511
BlsI GCNGC 9 cut(s) 343, 433, 624, 904, 978, 981, 1057, 1111, 1188
Bme1390I CCNGG 3 cut(s) 300, 309, 922
Bme18I GGWCC 2 cut(s) 385, 585
BmgT120I GGNCC 4 cut(s) 295, 296, 385, 585
BmiI GGNNCC 5 cut(s) 36, 296, 297, 586, 587
BmrFI CCNGG 3 cut(s) 300, 309, 922
BmsI GCATC 1 cut(s) 756
BpiI GAAGAC 1 cut(s) 310
Bpu14I TTCGAA 1 cut(s) 1096
BsaHI GRCGYC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 3 cut(s) 290, 298, 511
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 559
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 3 cut(s) 300, 309, 922
BseDI CCNNGG 3 cut(s) 290, 298, 511
BseGI GGATG 4 cut(s) 127, 387, 553, 793
BseLI CCNNNNNNNGG 2 cut(s) 155, 559
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 434, 974
BseNI ACTGG 1 cut(s) 464
BseSI GKGCMC 1 cut(s) 299
BseXI GCAGC 9 cut(s) 328, 418, 634, 914, 988, 991, 1067, 1096, 1198
BsgI GTGCAG 2 cut(s) 346, 641
Bsh1236I CGCG 1 cut(s) 40
BshFI GGCC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 3 cut(s) 296, 598, 1159
BslI CCNNNNNNNGG 2 cut(s) 155, 559
BsmAI GTCTC 3 cut(s) 603, 900, 1150
BsmFI GGGAC 3 cut(s) 296, 598, 1159
BsnI GGCC 1 cut(s) 297
Bso31I GGTCTC 1 cut(s) 1150
Bsp119I TTCGAA 1 cut(s) 1096
Bsp120I GGGCCC 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
Bsp19I CCATGG 1 cut(s) 290
BspACI CCGC 2 cut(s) 38, 643
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 2 cut(s) 433, 975
BspFNI CGCG 1 cut(s) 40
BspLI GGNNCC 5 cut(s) 36, 296, 297, 586, 587
BspMAI CTGCAG 2 cut(s) 978, 1057
BspMI ACCTGC 2 cut(s) 526, 1180
BspPI GGATC 1 cut(s) 653
BspT104I TTCGAA 1 cut(s) 1096
BspTNI GGTCTC 1 cut(s) 1150
BsrBI CCGCTC 1 cut(s) 643
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 3 cut(s) 290, 298, 511
BssMI GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
BssNI GRCGYC 1 cut(s) 160
BssT1I CCWWGG 2 cut(s) 290, 511
Bst2UI CCWGG 3 cut(s) 300, 309, 922
Bst4CI ACNGT 3 cut(s) 211, 287, 800
BstACI GRCGYC 1 cut(s) 160
BstBI TTCGAA 1 cut(s) 1096
BstC8I GCNNGC 5 cut(s) 20, 695, 879, 954, 1005
BstDEI CTNAG 2 cut(s) 420, 983
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 4 cut(s) 127, 387, 553, 793
BstFNI CGCG 1 cut(s) 40
BstKTI GATC 6 cut(s) 52, 66, 648, 793, 850, 1024
BstMAI GTCTC 3 cut(s) 603, 900, 1150
BstMBI GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
BstMWI GCNNNNNNNGC 3 cut(s) 242, 338, 962
BstNI CCWGG 3 cut(s) 300, 309, 922
BstNSI RCATGY 3 cut(s) 58, 104, 438
BstSCI CCNGG 3 cut(s) 298, 307, 920
BstSFI CTRYAG 3 cut(s) 483, 974, 1053
BstSLI GKGCMC 1 cut(s) 299
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 9 cut(s) 328, 418, 634, 914, 988, 991, 1067, 1096, 1198
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 645
BstYI RGATCY 1 cut(s) 645
BsuI GTATCC 2 cut(s) 456, 712
BsuRI GGCC 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 4 cut(s) 127, 387, 553, 793
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 3 cut(s) 139, 207, 334
BveI ACCTGC 2 cut(s) 526, 1180
Cac8I GCNNGC 5 cut(s) 20, 695, 879, 954, 1005
CaiI CAGNNNCTG 1 cut(s) 982
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 4 cut(s) 295, 296, 385, 585
CsiI ACCWGGT 1 cut(s) 307
Csp6I GTAC 2 cut(s) 1075, 1166
CviAII CATG 9 cut(s) 55, 101, 291, 334, 371, 404, 435, 698, 1152
CviQI GTAC 2 cut(s) 1075, 1166
DdeI CTNAG 2 cut(s) 420, 983
DpnI GATC 6 cut(s) 51, 65, 647, 792, 849, 1023
DpnII GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
Eco130I CCWWGG 2 cut(s) 290, 511
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 1150
Eco47I GGWCC 2 cut(s) 385, 585
Eco57I CTGAAG 2 cut(s) 621, 734
EcoO109I RGGNCCY 3 cut(s) 295, 296, 585
EcoRII CCWGG 3 cut(s) 298, 307, 920
EcoT14I CCWWGG 2 cut(s) 290, 511
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 2 cut(s) 290, 511
FaeI CATG 9 cut(s) 58, 104, 294, 337, 374, 407, 438, 701, 1155
FaqI GGGAC 3 cut(s) 296, 598, 1159
FatI CATG 9 cut(s) 54, 100, 290, 333, 370, 403, 434, 697, 1151
Fnu4HI GCNGC 9 cut(s) 342, 432, 623, 903, 977, 980, 1056, 1110, 1187
FokI GGATG 4 cut(s) 134, 394, 540, 800
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 9 cut(s) 342, 432, 623, 903, 977, 980, 1056, 1110, 1187
FspBI CTAG 1 cut(s) 512
GluI GCNGC 9 cut(s) 342, 432, 623, 903, 977, 980, 1056, 1110, 1187
HaeIII GGCC 1 cut(s) 297
HapII CCGG 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 9 cut(s) 58, 104, 294, 337, 374, 407, 438, 701, 1155
HincII GTYRAC 2 cut(s) 139, 708
HindII GTYRAC 2 cut(s) 139, 708
HinfI GANTC 6 cut(s) 204, 215, 488, 893, 970, 1119
HpaII CCGG 1 cut(s) 195
HphI GGTGA 2 cut(s) 209, 281
Hpy166II GTNNAC 4 cut(s) 139, 272, 440, 708
Hpy188I TCNGA 9 cut(s) 49, 423, 493, 616, 640, 663, 787, 826, 1087
Hpy188III TCNNGA 2 cut(s) 731, 1181
Hpy8I GTNNAC 4 cut(s) 139, 272, 440, 708
HpyAV CCTTC 4 cut(s) 36, 260, 286, 709
HpyCH4III ACNGT 3 cut(s) 211, 287, 800
HpyCH4IV ACGT 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 338, 962
HpyF3I CTNAG 2 cut(s) 420, 983
HpySE526I ACGT 1 cut(s) 160
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 9 cut(s) 58, 104, 294, 337, 374, 407, 438, 701, 1155
KflI GGGWCCC 1 cut(s) 585
Kzo9I GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
Lsp1109I GCAGC 9 cut(s) 328, 418, 634, 914, 988, 991, 1067, 1096, 1198
LweI GCATC 1 cut(s) 756
MabI ACCWGGT 1 cut(s) 307
MaeI CTAG 1 cut(s) 512
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 5 cut(s) 205, 353, 478, 772, 959
MalI GATC 6 cut(s) 51, 65, 647, 792, 849, 1023
MbiI CCGCTC 1 cut(s) 643
MboI GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
MboII GAAGA 7 cut(s) 194, 235, 315, 640, 785, 1031, 1075
MflI RGATCY 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 7 cut(s) 176, 414, 528, 1010, 1092, 1102, 1176
MlyI GAGTC 3 cut(s) 213, 482, 902
MmeI TCCRAC 1 cut(s) 765
MnlI CCTC 6 cut(s) 33, 371, 738, 879, 1036, 1179
MseI TTAA 4 cut(s) 78, 833, 932, 1013
MslI CAYNNNNRTG 4 cut(s) 71, 105, 408, 1127
MspA1I CMGCKG 2 cut(s) 982, 1163
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 3 cut(s) 300, 309, 922
MvaI CCWGG 3 cut(s) 300, 309, 922
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 3 cut(s) 242, 338, 962
NcoI CCATGG 1 cut(s) 290
NdeII GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
NlaIII CATG 9 cut(s) 58, 104, 294, 337, 374, 407, 438, 701, 1155
NlaIV GGNNCC 5 cut(s) 36, 296, 297, 586, 587
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 2 cut(s) 205, 478
NspI RCATGY 3 cut(s) 58, 104, 438
NspV TTCGAA 1 cut(s) 1096
PaqCI CACCTGC 1 cut(s) 1180
PfeI GAWTC 3 cut(s) 215, 970, 1119
PflMI CCANNNNNTGG 2 cut(s) 155, 559
PkrI GCNGC 9 cut(s) 343, 433, 624, 904, 978, 981, 1057, 1111, 1188
PleI GAGTC 3 cut(s) 212, 482, 901
PpsI GAGTC 3 cut(s) 212, 482, 901
PpuMI RGGWCCY 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 585
Psp6I CCWGG 3 cut(s) 298, 307, 920
PspGI CCWGG 3 cut(s) 298, 307, 920
PspN4I GGNNCC 5 cut(s) 36, 296, 297, 586, 587
PspOMI GGGCCC 1 cut(s) 295
PspPI GGNCC 4 cut(s) 295, 296, 385, 585
PspPPI RGGWCCY 1 cut(s) 585
PsrI GAACNNNNNNTAC 2 cut(s) 1058, 1090
PstI CTGCAG 2 cut(s) 978, 1057
PstNI CAGNNNCTG 1 cut(s) 982
PsuI RGATCY 1 cut(s) 645
PvuII CAGCTG 2 cut(s) 982, 1163
RsaI GTAC 2 cut(s) 1076, 1167
RsaNI GTAC 2 cut(s) 1075, 1166
RseI CAYNNNNRTG 4 cut(s) 71, 105, 408, 1127
SaqAI TTAA 4 cut(s) 78, 833, 932, 1013
SatI GCNGC 9 cut(s) 342, 432, 623, 903, 977, 980, 1056, 1110, 1187
Sau3AI GATC 6 cut(s) 49, 63, 645, 790, 847, 1021
Sau96I GGNCC 4 cut(s) 295, 296, 385, 585
SchI GAGTC 3 cut(s) 213, 482, 902
ScrFI CCNGG 3 cut(s) 300, 309, 922
SduI GDGCHC 1 cut(s) 299
SexAI ACCWGGT 1 cut(s) 307
SfaNI GCATC 1 cut(s) 756
SfcI CTRYAG 3 cut(s) 483, 974, 1053
SfuI TTCGAA 1 cut(s) 1096
SinI GGWCC 2 cut(s) 385, 585
SmiMI CAYNNNNRTG 4 cut(s) 71, 105, 408, 1127
Sse9I AATT 7 cut(s) 176, 414, 528, 1010, 1092, 1102, 1176
SsiI CCGC 2 cut(s) 38, 643
SspMI CTAG 1 cut(s) 512
StyD4I CCNGG 3 cut(s) 298, 307, 920
StyI CCWWGG 2 cut(s) 290, 511
TaaI ACNGT 3 cut(s) 211, 287, 800
TaiI ACGT 1 cut(s) 163
TaqI TCGA 5 cut(s) 190, 321, 503, 1041, 1096
TasI AATT 7 cut(s) 176, 414, 528, 1010, 1092, 1102, 1176
TfiI GAWTC 3 cut(s) 215, 970, 1119
Tru1I TTAA 4 cut(s) 78, 833, 932, 1013
Tru9I TTAA 4 cut(s) 78, 833, 932, 1013
TscAI CASTG 3 cut(s) 139, 214, 334
TseFI GTSAC 2 cut(s) 205, 478
TseI GCWGC 9 cut(s) 341, 431, 622, 902, 976, 979, 1055, 1109, 1186
Tsp45I GTSAC 2 cut(s) 205, 478
TspDTI ATGAA 2 cut(s) 426, 857
TspRI CASTG 3 cut(s) 139, 214, 334
Van91I CCANNNNNTGG 2 cut(s) 155, 559
VpaK11BI GGWCC 2 cut(s) 385, 585
XapI RAATTY 1 cut(s) 1102
XceI RCATGY 3 cut(s) 58, 104, 438
XmaJI CCTAGG 1 cut(s) 511
XspI CTAG 1 cut(s) 512
ZraI GACGTC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.