Rorug06G0273000

cysteine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000957
Physical Location & Seq
Reverse (-)
42043563 .. 42047202
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0273000.1

Sequence Viewer

Length: 1008 bp
ATGACGACGAGCAGTGACGTGGAGGCCGGATTCGCGAAGCTCCAAGGCGAAGACTTTGAGTACTACATGCAGACCTACTCCATAATCCTGGGCCGCAACTCCAAGAAATCGACGGTGGACGTCGACCTCTCCAGCCTCGGCGGCGGAATGAACATCTCCCGCCACCACGCGCGCATCTTCTACGACTTCACGCGCCGCCGCTTCGCTCTGGAGGTTCTCGGGAAGAACGGGTGTCTCGTCGAGGGGGTTCTCCACCTCCCCGGAAACCCTCCGGTCAAGCTCGATTCGCAGGATCTGCTTCAGATTGGGGATAAAGAGTTTTACTTTTTGTTGCCGGTGAGGAGTATTCTGGGTGGGCCTATTGGGCCCAGGCATTACGCCGCGGCGGTCGGGGTTTCGGGCTCGGCGGGCACGGCCCATTATGGATATGGGCATCCGGGCGCGTCGGCCGGGTCGGTGGCGAAGAAGAGTAGGGGGAGGGAGTTTTATGAGGACGAATATGAGGATGAGGATGATATTGGCGGCGGCGGCGGTGGTGGTAGTGGCAGTGGGAAGAAGAGGAGGGAAGGGTATGAGGGTTATGGGTACAGTGGTGGGCCGGGTTCTGGATCGGGCACCAAGTCGGGTTCTTTGGCTTTAGAAAAGAAGGGAGATGGAAGATCAAGGGTTGATCGAGACAGTGACAATCAGCAACTTCTGCATTTGGAAGAAAAGGATGTGGTGTCATCTGTTGCTACGGTGCTCTCTGATCTTTGTGGTCCTGGCGAATGGATGCCTATGGAGAAACTGCATGCTGAGTTGGTGGAACAGTATAGTGGTGTTTGGCATCACACTCGAGTGAGGAGATATCTTACTTCTGAGGACTGGCCTGGTCCCGAATCAAAGGGGAAACCATGGTACGGCTTGCTTATGCTGCTAAGGAAATACCCAGAACATTTTGTCATCAACACAAGGTCTAAGGGTCGTGTAACTCTGGAATTTGTATCTCTTGTTTCGTTGATTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

335

Amino Acids

36.52

Weight (kDa)

6.43

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 29 - 103 2.7e-10 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 123
AccII CGCG 6 cut(s) 35, 170, 172, 193, 383, 443
AclWI GGATC 2 cut(s) 300, 616
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 1 cut(s) 977
AcuI CTGAAG 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 3 cut(s) 62, 587, 899
AfiI CCNNNNNNNGG 2 cut(s) 605, 899
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 4 cut(s) 87, 368, 760, 868
AleI CACNNNNGTG 1 cut(s) 836
AluBI AGCT 2 cut(s) 40, 280
AluI AGCT 2 cut(s) 40, 280
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 2 cut(s) 239, 669
AlwI GGATC 2 cut(s) 300, 616
AlwNI CAGNNNCTG 1 cut(s) 295
Ama87I CYCGRG 2 cut(s) 218, 834
AoxI GGCC 8 cut(s) 24, 91, 356, 365, 414, 447, 596, 866
ApaI GGGCCC 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 1 cut(s) 977
AspLEI GCGC 4 cut(s) 172, 174, 195, 443
AspS9I GGNCC 8 cut(s) 91, 356, 365, 366, 415, 596, 758, 872
AsuC2I CCSGG 4 cut(s) 261, 438, 451, 600
AsuHPI GGTGA 1 cut(s) 349
AvaI CYCGRG 2 cut(s) 218, 834
AvaII GGWCC 2 cut(s) 758, 872
BaeGI GKGCMC 3 cut(s) 369, 413, 617
BaeI ACNNNNGTAYC 2 cut(s) 577, 610
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 2 cut(s) 369, 404
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 1 cut(s) 900
BccI CCATC 1 cut(s) 647
BceAI ACGGC 2 cut(s) 429, 916
BcgI CGANNNNNNTGC 2 cut(s) 815, 849
BciT130I CCWGG 4 cut(s) 89, 370, 762, 870
BcnI CCSGG 4 cut(s) 261, 438, 451, 600
BcoDI GTCTC 2 cut(s) 239, 669
BglI GCCNNNNNGGC 2 cut(s) 141, 364
BmcAI AGTACT 1 cut(s) 62
Bme1390I CCNGG 8 cut(s) 89, 261, 370, 438, 451, 600, 762, 870
Bme18I GGWCC 2 cut(s) 758, 872
BmeT110I CYCGRG 2 cut(s) 218, 834
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 8 cut(s) 91, 356, 365, 366, 415, 596, 758, 872
BmiI GGNNCC 3 cut(s) 367, 616, 874
BmrFI CCNGG 8 cut(s) 89, 261, 370, 438, 451, 600, 762, 870
BmsI GCATC 4 cut(s) 183, 442, 762, 835
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 2 cut(s) 115, 230
Bpu10I CCTNAGC 1 cut(s) 917
BpuMI CCSGG 4 cut(s) 261, 438, 451, 600
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 7 cut(s) 43, 88, 136, 259, 368, 381, 893
BsaWI WCCGGW 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 605, 899
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 869
BseBI CCWGG 4 cut(s) 89, 370, 762, 870
BseDI CCNNGG 7 cut(s) 43, 88, 136, 259, 368, 381, 893
BseGI GGATG 5 cut(s) 433, 511, 517, 721, 777
BseLI CCNNNNNNNGG 2 cut(s) 605, 899
BseMII CTCAG 2 cut(s) 786, 849
BseNI ACTGG 1 cut(s) 869
BsePI GCGCGC 1 cut(s) 170
BseRI GAGGAG 3 cut(s) 355, 574, 856
BseSI GKGCMC 3 cut(s) 369, 413, 617
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 1 cut(s) 900
Bsh1236I CGCG 6 cut(s) 35, 170, 172, 193, 383, 443
Bsh1285I CGRYCG 2 cut(s) 390, 450
BshFI GGCC 8 cut(s) 26, 93, 358, 367, 416, 449, 598, 868
BshNI GGYRCC 1 cut(s) 614
BsiEI CGRYCG 2 cut(s) 390, 450
BsiHKAI GWGCWC 1 cut(s) 744
BsiHKCI CYCGRG 2 cut(s) 218, 834
BsiSI CCGG 7 cut(s) 27, 261, 272, 335, 437, 450, 599
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 2 cut(s) 605, 899
BsmAI GTCTC 2 cut(s) 239, 669
BsmFI GGGAC 1 cut(s) 858
BsnI GGCC 8 cut(s) 26, 93, 358, 367, 416, 449, 598, 868
BsoBI CYCGRG 2 cut(s) 218, 834
Bsp120I GGGCCC 1 cut(s) 365
Bsp1286I GDGCHC 5 cut(s) 369, 404, 413, 617, 744
Bsp143I GATC 5 cut(s) 292, 608, 659, 670, 748
Bsp19I CCATGG 1 cut(s) 893
Bsp68I TCGCGA 1 cut(s) 35
BspANI GGCC 8 cut(s) 26, 93, 358, 367, 416, 449, 598, 868
BspCNI CTCAG 2 cut(s) 787, 850
BspFNI CGCG 6 cut(s) 35, 170, 172, 193, 383, 443
BspLI GGNNCC 3 cut(s) 367, 616, 874
BspPI GGATC 2 cut(s) 300, 616
BspT107I GGYRCC 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 1 cut(s) 869
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 7 cut(s) 43, 88, 136, 259, 368, 381, 893
BssHII GCGCGC 1 cut(s) 170
BssMI GATC 5 cut(s) 292, 608, 659, 670, 748
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 2 cut(s) 43, 893
Bst2UI CCWGG 4 cut(s) 89, 370, 762, 870
Bst4CI ACNGT 5 cut(s) 115, 590, 680, 739, 810
Bst6I CTCTTC 2 cut(s) 461, 551
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 2 cut(s) 295, 697
BstC8I GCNNGC 4 cut(s) 172, 409, 792, 905
BstDEI CTNAG 4 cut(s) 795, 858, 917, 957
BstDSI CCRYGG 2 cut(s) 381, 893
BstF5I GGATG 5 cut(s) 433, 511, 517, 721, 777
BstFNI CGCG 6 cut(s) 35, 170, 172, 193, 383, 443
BstHHI GCGC 4 cut(s) 172, 174, 195, 443
BstKTI GATC 5 cut(s) 295, 611, 662, 673, 751
BstMAI GTCTC 2 cut(s) 239, 669
BstMBI GATC 5 cut(s) 292, 608, 659, 670, 748
BstMCI CGRYCG 2 cut(s) 390, 450
BstNI CCWGG 4 cut(s) 89, 370, 762, 870
BstNSI RCATGY 2 cut(s) 70, 794
BstSCI CCNGG 8 cut(s) 87, 259, 368, 436, 449, 598, 760, 868
BstSLI GKGCMC 3 cut(s) 369, 413, 617
BstUI CGCG 6 cut(s) 35, 170, 172, 193, 383, 443
BstV1I GCAGC 1 cut(s) 900
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 292
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 292
BstZI CGGCCG 1 cut(s) 447
BsuRI GGCC 8 cut(s) 26, 93, 358, 367, 416, 449, 598, 868
BtgI CCRYGG 2 cut(s) 381, 893
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 5 cut(s) 433, 511, 517, 721, 777
BtsI GCAGTG 2 cut(s) 19, 553
BtsIMutI CAGTG 4 cut(s) 19, 553, 595, 685
BtuMI TCGCGA 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 172, 409, 792, 905
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 4 cut(s) 172, 174, 195, 443
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 8 cut(s) 91, 356, 365, 366, 415, 596, 758, 872
Cfr42I CCGCGG 1 cut(s) 384
CseI GACGC 1 cut(s) 432
Csp6I GTAC 3 cut(s) 61, 586, 898
CviAII CATG 3 cut(s) 67, 791, 894
CviQI GTAC 3 cut(s) 61, 586, 898
DdeI CTNAG 4 cut(s) 795, 858, 917, 957
DpnI GATC 5 cut(s) 294, 610, 661, 672, 750
DpnII GATC 5 cut(s) 292, 608, 659, 670, 748
EaeI YGGCCR 1 cut(s) 447
EagI CGGCCG 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 461, 551
EarI CTCTTC 2 cut(s) 461, 551
EciI GGCGGA 1 cut(s) 159
EclXI CGGCCG 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 43, 893
Eco24I GRGCYC 2 cut(s) 369, 404
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 758, 872
Eco52I CGGCCG 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 218, 834
EcoRII CCWGG 4 cut(s) 87, 368, 760, 868
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 2 cut(s) 43, 893
EcoT38I GRGCYC 2 cut(s) 369, 404
ErhI CCWWGG 2 cut(s) 43, 893
FaeI CATG 3 cut(s) 70, 794, 897
FaqI GGGAC 1 cut(s) 858
FatI CATG 3 cut(s) 66, 790, 893
FauI CCCGC 2 cut(s) 167, 400
FblI GTMKAC 1 cut(s) 123
FokI GGATG 5 cut(s) 420, 518, 524, 728, 784
FriOI GRGCYC 2 cut(s) 369, 404
GlaI GCGC 4 cut(s) 171, 173, 194, 442
GsuI CTGGAG 2 cut(s) 115, 230
HaeIII GGCC 8 cut(s) 26, 93, 358, 367, 416, 449, 598, 868
HapII CCGG 7 cut(s) 27, 261, 272, 335, 437, 450, 599
HgaI GACGC 1 cut(s) 432
HhaI GCGC 4 cut(s) 172, 174, 195, 443
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 3 cut(s) 70, 794, 897
Hin6I GCGC 4 cut(s) 170, 172, 193, 441
HinP1I GCGC 4 cut(s) 170, 172, 193, 441
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 3 cut(s) 30, 284, 878
HpaII CCGG 7 cut(s) 27, 261, 272, 335, 437, 450, 599
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 2 cut(s) 118, 124
Hpy188I TCNGA 3 cut(s) 303, 748, 859
Hpy188III TCNNGA 7 cut(s) 34, 209, 220, 606, 674, 875, 974
Hpy8I GTNNAC 2 cut(s) 118, 124
Hpy99I CGWCG 5 cut(s) 10, 115, 125, 242, 448
HpyAV CCTTC 2 cut(s) 560, 640
HpyCH4III ACNGT 5 cut(s) 115, 590, 680, 739, 810
HpyCH4IV ACGT 2 cut(s) 18, 120
HpyCH4V TGCA 3 cut(s) 70, 700, 790
HpyF3I CTNAG 4 cut(s) 795, 858, 917, 957
HpySE526I ACGT 2 cut(s) 18, 120
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 3 cut(s) 70, 794, 897
HspAI GCGC 4 cut(s) 170, 172, 193, 441
KspI CCGCGG 1 cut(s) 384
Kzo9I GATC 5 cut(s) 292, 608, 659, 670, 748
LmnI GCTCC 1 cut(s) 45
Lsp1109I GCAGC 1 cut(s) 900
LweI GCATC 4 cut(s) 183, 442, 762, 835
MaeII ACGT 2 cut(s) 18, 120
MaeIII GTNAC 3 cut(s) 14, 680, 967
MalI GATC 5 cut(s) 294, 610, 661, 672, 750
MboI GATC 5 cut(s) 292, 608, 659, 670, 748
MboII GAAGA 9 cut(s) 62, 169, 235, 475, 478, 565, 568, 669, 719
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 5 cut(s) 369, 404, 413, 617, 744
MluCI AATT 1 cut(s) 977
MslI CAYNNNNRTG 1 cut(s) 836
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 7 cut(s) 27, 261, 272, 335, 437, 450, 599
MspR9I CCNGG 8 cut(s) 89, 261, 370, 438, 451, 600, 762, 870
MvaI CCWGG 4 cut(s) 89, 370, 762, 870
MvnI CGCG 6 cut(s) 35, 170, 172, 193, 383, 443
NciI CCSGG 4 cut(s) 261, 438, 451, 600
NcoI CCATGG 1 cut(s) 893
NdeII GATC 5 cut(s) 292, 608, 659, 670, 748
NlaIII CATG 3 cut(s) 70, 794, 897
NlaIV GGNNCC 3 cut(s) 367, 616, 874
NmeAIII GCCGAG 2 cut(s) 117, 383
NmuCI GTSAC 2 cut(s) 14, 680
NruI TCGCGA 1 cut(s) 35
NspI RCATGY 2 cut(s) 70, 794
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 834
PauI GCGCGC 1 cut(s) 170
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 3 cut(s) 30, 284, 878
Psp6I CCWGG 4 cut(s) 87, 368, 760, 868
PspGI CCWGG 4 cut(s) 87, 368, 760, 868
PspN4I GGNNCC 3 cut(s) 367, 616, 874
PspOMI GGGCCC 1 cut(s) 365
PspPI GGNCC 8 cut(s) 91, 356, 365, 366, 415, 596, 758, 872
PspXI VCTCGAGB 1 cut(s) 834
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 292
PteI GCGCGC 1 cut(s) 170
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 3 cut(s) 62, 587, 899
RsaNI GTAC 3 cut(s) 61, 586, 898
RseI CAYNNNNRTG 1 cut(s) 836
SacII CCGCGG 1 cut(s) 384
SalI GTCGAC 1 cut(s) 122
Sau3AI GATC 5 cut(s) 292, 608, 659, 670, 748
Sau96I GGNCC 8 cut(s) 91, 356, 365, 366, 415, 596, 758, 872
ScaI AGTACT 1 cut(s) 62
ScrFI CCNGG 8 cut(s) 89, 261, 370, 438, 451, 600, 762, 870
SduI GDGCHC 5 cut(s) 369, 404, 413, 617, 744
SetI ASST 9 cut(s) 21, 42, 77, 123, 129, 216, 258, 282, 956
SfaNI GCATC 4 cut(s) 183, 442, 762, 835
SfiI GGCCNNNNNGGCC 1 cut(s) 364
Sfr274I CTCGAG 1 cut(s) 834
Sfr303I CCGCGG 1 cut(s) 384
SgrBI CCGCGG 1 cut(s) 384
SinI GGWCC 2 cut(s) 758, 872
SlaI CTCGAG 1 cut(s) 834
SmiMI CAYNNNNRTG 1 cut(s) 836
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
SphI GCATGC 1 cut(s) 794
Sse9I AATT 1 cut(s) 977
StyD4I CCNGG 8 cut(s) 87, 259, 368, 436, 449, 598, 760, 868
StyI CCWWGG 2 cut(s) 43, 893
TaaI ACNGT 5 cut(s) 115, 590, 680, 739, 810
TaiI ACGT 2 cut(s) 21, 123
TaqI TCGA 6 cut(s) 110, 123, 240, 282, 673, 835
TasI AATT 1 cut(s) 977
TatI WGTACW 1 cut(s) 60
TauI GCSGC 9 cut(s) 96, 144, 198, 201, 383, 386, 525, 528, 531
TfiI GAWTC 3 cut(s) 30, 284, 878
TscAI CASTG 4 cut(s) 19, 553, 595, 685
TseFI GTSAC 2 cut(s) 14, 680
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 2 cut(s) 14, 680
TspDTI ATGAA 2 cut(s) 164, 993
TspRI CASTG 4 cut(s) 19, 553, 595, 685
VpaK11BI GGWCC 2 cut(s) 758, 872
XapI RAATTY 1 cut(s) 977
XceI RCATGY 2 cut(s) 70, 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 834
XmiI GTMKAC 1 cut(s) 123
ZraI GACGTC 1 cut(s) 121
ZrmI AGTACT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.