Rh3BG323600

cysteine--tRNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000957
Physical Location & Seq
Forward (+)
33495126 .. 33498684
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG323600.1

Sequence Viewer

Length: 504 bp
ATGGGCACTGGAAGACCTGGTTGGCATATCGAGTGCAGTGCCATGAGTACTGCTTATCTGGGTTACTCTTTTGACATACATGGTGGAGAAATGGACCTTCTGTTTCCCCCACATGAGAATGAAATTGCTCAGAGTTGTGCTGCTTGTAAACAAAGTTATATAAGCTACTGGATACACAATGGTTTTGTCACTATAGACTCTGAAAAAATGGTCAAATCACTATGGAACTTTTTTACAATTCGACAGATCAAGGAGACGAAGTTGTTGAAGCTGATGGAGGAGATGGTGGTGCTTCGGTTATCCACCAAAGTGAAACAAGAAAGTAGACCATACCCAGATCGGGGGGAGGTGAGCGAGGATTTGAAAGTAAGGTTTTCGAGGGCAATTGGGAGCCCCGCAGTAGCCTTGATTACCCACTTATTTGGGAGGAGCGCTCCTAGATTCGAGACAATTTTGGGTCAACCTCCGATCTGTCCAGAATGTTTCATCCCCCTACAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000741 GO:0003674 GO:0003824 GO:0004812 GO:0004817 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006417 GO:0006418 GO:0006423 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0006996 GO:0006997 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009559 GO:0009561 GO:0009570 GO:0009889 GO:0009987 GO:0010197 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0010941 GO:0016043 GO:0016070 GO:0016874 GO:0016875 GO:0017076 GO:0019222 GO:0019538 GO:0019752 GO:0030154 GO:0030554 GO:0031323 GO:0031326 GO:0032268 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0034248 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042407 GO:0043038 GO:0043039 GO:0043043 GO:0043067 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0060255 GO:0061024 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
DALR_2 tRNA-synt_1e tRNA-synt_1g

Protein Analysis

167

Amino Acids

18.96

Weight (kDa)

6.52

Isoelectric Point (pI)

70.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1e PF01406 2 - 85 7.7e-39 tRNA synthetases class I (C) catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 325
AciI CCGC 1 cut(s) 396
AfaI GTAC 1 cut(s) 49
AfeI AGCGCT 1 cut(s) 433
AfiI CCNNNNNNNGG 3 cut(s) 340, 341, 496
AgsI TTSAA 2 cut(s) 268, 364
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 2 cut(s) 165, 271
AluI AGCT 2 cut(s) 165, 271
Alw26I GTCTC 2 cut(s) 248, 440
Aor51HI AGCGCT 1 cut(s) 433
ApeKI GCWGC 1 cut(s) 140
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 1 cut(s) 361
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 268, 277
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 248, 440
BfaI CTAG 2 cut(s) 438, 502
BfmI CTRYAG 2 cut(s) 192, 494
BfoI RGCGCY 1 cut(s) 435
BfuI GTATCC 1 cut(s) 165
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 19
BplI GAGNNNNNCTC 2 cut(s) 418, 450
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 341, 496
Bse1I ACTGG 2 cut(s) 13, 173
BseBI CCWGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 340, 341, 496
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 13, 173
BseRI GAGGAG 2 cut(s) 293, 442
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 55
BslI CCNNNNNNNGG 3 cut(s) 340, 341, 496
BsmAI GTCTC 2 cut(s) 248, 440
BsmBI CGTCTC 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 8, 395
Bsp143I GATC 3 cut(s) 246, 337, 468
BspACI CCGC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 392
BsrI ACTGG 2 cut(s) 13, 173
BssMI GATC 3 cut(s) 246, 337, 468
Bst2UI CCWGG 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 486
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 3 cut(s) 249, 340, 471
BstMAI GTCTC 2 cut(s) 248, 440
BstMBI GATC 3 cut(s) 246, 337, 468
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 2 cut(s) 192, 494
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 19
BstXI CCANNNNNNTGG 1 cut(s) 422
BsuI GTATCC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 486
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 6, 43
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 94
CsiI ACCWGGT 1 cut(s) 16
Csp6I GTAC 1 cut(s) 48
CviAII CATG 3 cut(s) 43, 80, 113
CviJI RGCY 4 cut(s) 165, 271, 393, 404
CviKI_1 RGCY 4 cut(s) 165, 271, 393, 404
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 129
DpnI GATC 3 cut(s) 248, 339, 470
DpnII GATC 3 cut(s) 246, 337, 468
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 94
Eco47III AGCGCT 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 395
Esp3I CGTCTC 1 cut(s) 248
FaeI CATG 3 cut(s) 46, 83, 116
FatI CATG 3 cut(s) 42, 79, 112
FauI CCCGC 1 cut(s) 403
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 2 cut(s) 438, 502
GlaI GCGC 1 cut(s) 433
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 435
HhaI GCGC 1 cut(s) 434
Hin1II CATG 3 cut(s) 46, 83, 116
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 461
HindII GTYRAC 1 cut(s) 461
HinfI GANTC 2 cut(s) 197, 441
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 3 cut(s) 149, 326, 461
Hpy188I TCNGA 3 cut(s) 132, 202, 468
Hpy188III TCNNGA 2 cut(s) 445, 476
Hpy8I GTNNAC 3 cut(s) 149, 326, 461
HpyAV CCTTC 1 cut(s) 107
HpyCH4V TGCA 1 cut(s) 36
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 3 cut(s) 46, 83, 116
HspAI GCGC 1 cut(s) 432
Kzo9I GATC 3 cut(s) 246, 337, 468
LmnI GCTCC 3 cut(s) 390, 429, 439
LpnPI CCDG 7 cut(s) 3, 30, 44, 154, 348, 482, 489
Lsp1109I GCAGC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 2 cut(s) 438, 502
MaeIII GTNAC 2 cut(s) 62, 187
MalI GATC 3 cut(s) 248, 339, 470
MboI GATC 3 cut(s) 246, 337, 468
MboII GAAGA 1 cut(s) 24
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 2 cut(s) 8, 395
MluCI AATT 4 cut(s) 123, 237, 384, 450
MlyI GAGTC 1 cut(s) 191
MnlI CCTC 6 cut(s) 271, 340, 349, 372, 420, 474
MslI CAYNNNNRTG 2 cut(s) 117, 308
MspR9I CCNGG 1 cut(s) 18
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 18
NdeII GATC 3 cut(s) 246, 337, 468
NlaIII CATG 3 cut(s) 46, 83, 116
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 187
OliI CACNNNNGTG 1 cut(s) 308
PfeI GAWTC 1 cut(s) 441
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 1 cut(s) 94
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 2 cut(s) 117, 308
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 3 cut(s) 246, 337, 468
Sau96I GGNCC 1 cut(s) 94
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 2 cut(s) 8, 395
SetI ASST 7 cut(s) 19, 99, 167, 273, 351, 374, 466
SexAI ACCWGGT 1 cut(s) 16
SfcI CTRYAG 2 cut(s) 192, 494
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 117, 308
Sse9I AATT 4 cut(s) 123, 237, 384, 450
SsiI CCGC 1 cut(s) 396
SspMI CTAG 2 cut(s) 438, 502
StyD4I CCNGG 1 cut(s) 16
TaqI TCGA 4 cut(s) 30, 241, 377, 444
TasI AATT 4 cut(s) 123, 237, 384, 450
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 1 cut(s) 441
TscAI CASTG 2 cut(s) 13, 43
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 135, 475
TspRI CASTG 2 cut(s) 13, 43
VpaK11BI GGWCC 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 325
XspI CTAG 2 cut(s) 438, 502
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.